scholarly journals Hepatoprotective activity of aqueous extract from Caragana jubata (Pall.) Poir. shoots in the model of acute hepatitis induced by acetaminophen in rats

2018 ◽  
Vol 64 (3) ◽  
pp. 241-246 ◽  
Author(s):  
P.A. Kakorin ◽  
I.V. Babenkova ◽  
Y.O. Teselkin ◽  
G.V. Ramenskaya ◽  
T.A. Demura ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Alkaline Phosphatase ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Total Bilirubin ◽  
Histological Study ◽  
Blood Parameters ◽  
Hepatoprotective Activity ◽  
Reference Preparation ◽  
Liver Homogenates

The aim of the study was to investigate the hepatoprotective activity of an aqueous extract of Caragana jubata (Pall.) Poir. Acute experimental hepatitis was induced by acetaminophen administration of 1000 mg/kg. Studies were conducted in white Wistar rats. The aqueous extract of C. jubata demonstrated the hepatoprotective effect, comparable to that of the reference preparation “Carcil”. This was manifested by the normalization of biochemical blood parameters (ALT, AST, alkaline phosphatase, cholesterol, total bilirubin) and antioxidant activity of liver homogenates, determined by the method based on oxidation of luminol induced by 2,2¢-azo-bis-2-amidinopropane. Normalization of morphofunctional indices was also shown in a histological study of liver of rats that received aqueous extract from C. jubata.

Histopathological and biochemical assessments of Costus afer stem on alloxan-induced diabetic rats

2017 ◽  
Vol 28 (4) ◽  
Author(s):  
Anthoneth Ndidi Ezejiofor ◽  
Zelinjo Nkeiruka Igweze ◽  
Nnaemeka Arinze Udowelle ◽  
Orish Ebere Orisakwe
Keyword(s):  
Alkaline Phosphatase ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Total Bilirubin ◽  
Diabetic Rats ◽  
Control Group ◽  
Marker Enzymes ◽  
Hypoglycaemic Effect ◽  
Stem Extract ◽  
Costus Afer

AbstractBackground:In continuation of our study of the biological activity of the aqueous extract ofMethods:Thirty male albino Wistar rats (150−200 g) were weight-matched into six groups of five rats each. Groups 1 and 2 were the normal (non-induced) and toxic (alloxan-induced) controls, respectively. Groups 3−6 were induced and treated with 1, 2 and 3 g/kg of the aqueous extract ofResults:There was a significant reduction in alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin triglyceride, creatinine, urea and electrolyte when compared with toxic control group. The liver marker enzymes were restored in all the treated groups. The aqueous stem extract possessed hypoglycaemic effect and reversed the histopathological damage in alloxan-induced diabetic rats.Conclusions:This study suggests that aqueous stem extract of

scholarly journals PHYTOCHEMICAL ANALYSIS, ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITY OF ACTINIOPTERIS RADIATA

2020 ◽  
pp. 69-74
Author(s):  
KANDUKURI GOURI SANKAR ◽  
BENDI SRI VENKATESWARLU
Keyword(s):  
Antioxidant Activity ◽  
Alkaline Phosphatase ◽  
Free Radical ◽  
Medicinal Plants ◽  
Total Bilirubin ◽  
Liver Toxicity ◽  
Hepatoprotective Activity ◽  
Phytochemical Analysis ◽  
Alcoholic Extract ◽  
Phytochemical Constituents

Objective: The medicinal plants have been using to treat ailments since ancient times. The recent advances in science and technology impel humans to evaluate medicinal plants therapeutic efficiency and isolation of bioactive compounds in pure forms before their use in development of new drugs and their derivatives. But even now, abundant medicinal plants unevaluated scientifically. The current study was aimed to explore phytochemical constituents, antioxidant and hepatoprotective activities of Actiniopteris radiata root parts. Methods: Standard procedures have been used to perform phytochemical analysis. Antioxidant activity was carried using In vitro methods on superoxide, hydroxyl, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. Hepatoprotective activity was studied by paracetamol-induced liver toxicity on WISTAR albino rats. The parameters assessed were Aspartate aminotransferase (SGOT/AST), Alanine aminotransferase (SGPT/ALT), alkaline phosphatase (ALP) and total bilirubin levels. Results: The tested extracts (hexane, ethyl acetate, and hydro-alcoholic) possess biologically active compounds such as sterols, terpenoids, glycosides, phenolics, alkaloids, flavonoids. The hydro-alcoholic extract has more phenolic contents (24.28±0.3) and flavonoid contents (22.68±0.6). The extracts showed dose dependent activity on tested free radicals and extracts showed more percentage inhibition at 320µg. The hydro-alcoholic extract showed more percentage inhibition i.e. 71.00±2.08 on DPPH free radical, 79.67±1.20 on hydroxyl free radical and 80.33±1.20 on superoxide free radical. As antioxidant activity of hexane and ethyl acetate extracts was less and they also showed less percentage protection on liver toxicity, hydro-alcoholic extract showed more percentage protection on biomedical enzyme levels of liver toxicity at high concentration i.e., 400 mg/kg b.w. The percentage protection on the enhancement of AST (SGOT), ALT (SGPT), ALP, and total bilirubin levels were 82.24%, 82.14%, 84.18%, and 82.85% are significant (P<0.01) as Liv52 shown percentage protection on the enhancement of Aspartate aminotransferase (SGOT), alanine aminotransferase (SGPT), Alkaline phosphatase (ALP) and total bilirubin levels were 93.58%, 92.83%, 94.67% and 93.57%. Conclusion: The current study was aimed to explore phytochemical constituents, antioxidant and hepatoprotective activities of Actiniopteris radiata root parts extracts. The outcome of the current research results provides scientific evidence of the traditional usage of Actiniopteris radiata.

scholarly journals Effect of aqueous extract of Azadirachta indica on rifampicin- induced jaundice in rabbits

2020 ◽  
Vol 8 (2) ◽  
pp. 205-212
Author(s):  
Tunji Akande ◽  
Timothy Olugbenga Ogundeko ◽  
Solomon Oyewale Opajobi ◽  
Samaila Garba Yaura ◽  
Isiaiah Iorkyase Idyu ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Aqueous Extract ◽  
Azadirachta Indica ◽  
Total Bilirubin ◽  
Alanine Transaminase ◽  
The Body ◽  
High Rate ◽  
Care Needs ◽  
Conjugated Bilirubin

High rate of dependence on medicinal plants for maintenance of health care needs over the decades in communities all over the world especially in Africa and Asia needs adequate scientific verification and authentication. Liver related diseases have however become a global problem. Jaundice was established on 9 of a total population of 12 rabbits of mixed sex, weighing 1.50kg - 2.34kg using an overdose pretreatment of Rifampicin (300mg/ kg bw) P.O. After 3 days, the Animals were administered with various doses of aqueous extract of Azadirachta indica (600, 750, 900mg/kg bw) P.O for each group of rats – A, B, C and D (control ), after which the animals were bled via marginal ear vein at zero hour, 7th and 14th days and the blood used for the following assays: Alkaline Phosphatase, Acid Phosphatase, Total Bilirubin, Conjugated Bilirubin and Alanine Transaminase. Experimental animals exhibited lack of appetite, reduced activity, scratching of the body, emesis, yellow coloration of the sclera, light-colored stool and a little dark urine over time. There was also increase in the levels of the biochemical parameters as the day increased. Aqueous extract of Azadirachta indica (Neem) exhibited increasing elevated levels of Alkaline Phosphatase (ALP), Acid Phosphatase (AP), Total Bilirubin (T/Bil), Conjugated Bilirubin (Conj/Bil), and slightly in Alanine Transaminase (ALT), thus exerted elevated hepatotoxicity on Rifampicin-induced Rabbits. Therefore, the traditional use of Azadirachta indica in management of jaundice should be discontinued.

scholarly journals Effect of aqueous extract of Azadirachta indica on rifampicin- induced jaundice in rabbits

2020 ◽  
Vol 8 (2) ◽  
pp. 205-212
Author(s):  
Tunji Akande ◽  
Timothy Olugbenga Ogundeko ◽  
Solomon Oyewale Opajobi ◽  
Samaila Garba Yaura ◽  
Isiaiah Iorkyase Idyu ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Aqueous Extract ◽  
Azadirachta Indica ◽  
Total Bilirubin ◽  
Alanine Transaminase ◽  
The Body ◽  
High Rate ◽  
Care Needs ◽  
Conjugated Bilirubin

High rate of dependence on medicinal plants for maintenance of health care needs over the decades in communities all over the world especially in Africa and Asia needs adequate scientific verification and authentication. Liver related diseases have however become a global problem. Jaundice was established on 9 of a total population of 12 rabbits of mixed sex, weighing 1.50kg - 2.34kg using an overdose pretreatment of Rifampicin (300mg/ kg bw) P.O. After 3 days, the Animals were administered with various doses of aqueous extract of Azadirachta indica (600, 750, 900mg/kg bw) P.O for each group of rats – A, B, C and D (control ), after which the animals were bled via marginal ear vein at zero hour, 7th and 14th days and the blood used for the following assays: Alkaline Phosphatase, Acid Phosphatase, Total Bilirubin, Conjugated Bilirubin and Alanine Transaminase. Experimental animals exhibited lack of appetite, reduced activity, scratching of the body, emesis, yellow coloration of the sclera, light-colored stool and a little dark urine over time. There was also increase in the levels of the biochemical parameters as the day increased. Aqueous extract of Azadirachta indica (Neem) exhibited increasing elevated levels of Alkaline Phosphatase (ALP), Acid Phosphatase (AP), Total Bilirubin (T/Bil), Conjugated Bilirubin (Conj/Bil), and slightly in Alanine Transaminase (ALT), thus exerted elevated hepatotoxicity on Rifampicin-induced Rabbits. Therefore, the traditional use of Azadirachta indica in management of jaundice should be discontinued.

scholarly journals HEPATOPROTECTIVE ACTIVITY OF CORDIA OBLIQUA AGAINST PARACETAMOL-INDUCED LIVER TOXICITY ON WISTAR RATS

2019 ◽  
pp. 149-152
Author(s):  
SIVAKRISHNAN SIVAGNANAM ◽  
PRADEEPRAJ DEVARASU
Keyword(s):  
Wistar Rats ◽  
Liver Toxicity ◽  
Histological Study ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Plant Materials ◽  
Percolation Method ◽  
Soxhlet Apparatus ◽  
Serum Glutamate Pyruvate Transaminase

Objective: The ethanolic extract of Cordia obliqua (EECO) Willd. leaves was evaluated for hepatoprotective activity in Wistar rats by inducing hepatic damage with paracetamol. Methods: The leaves of C. obliqua were dried under shade, segregated, pulverized by a mechanical grinder and passed through a 40 mesh sieve. The powdered plant materials were stored in an air-tight container. The above-powdered materials were successively extracted with ethanol by hot continuous percolation method in Soxhlet apparatus for 24 h. The extract was concentrated using a rotary evaporator and subjected to freeze drying in a lyophilizer till dry powder was obtained. Results: Treatment with EECO at 200 and 400 mg/kg showed significant decrease in serum glutamate-pyruvate transaminase, serum glutamic oxaloacetic transaminase, alkaline phosphatase, and total bilirubin levels and a significant elevation in the total protein, albumin, and globulin levels in serum when compared with paracetamol treated rats. In histological study of liver, the hepatocytes show moderate cytoplasm and moderately enlarged pleomorphic and hyperchromatic nuclei. The portal triads show mild periportal inflammation composed of lymphocytes, and central veins are normal. Conclusion: On the basis of results, we can conclude that the EECO leaves showed potential hepatoprotective activity in rats.

scholarly journals Metabolic status of rabbits under physiological adaptation to cage husbandry technology

2021 ◽  
Vol 37 ◽  
pp. 00047
Author(s):  
Natalia Khokhlova ◽  
Vladimir Semenyutin ◽  
Viktor Eremenko
Keyword(s):  
Alkaline Phosphatase ◽  
Structural Changes ◽  
Total Bilirubin ◽  
Physiological State ◽  
Blood Parameters ◽  
Physiological Adaptation ◽  
Adaptation Process ◽  
Plantar Surface ◽  
Regulatory Processes ◽  
Hind Limbs

The article considers changes in biochemical blood parameters depending on the degree of adaptation process manifesting on the plantar surface of hind limbs of different sex rabbits and physiological state under cage husbandry technology. Groups of stud bucks (1st series), pregnant and lactating does (2nd and 3rd series of experiments) were formed according to the stages of structural changes depending on the stress of physiological regulatory processes of adaptation (the systematics proposed by R.M. Baevsky). The authors studied the following factors in serum: total protein, protein fractions (albumin, α-, β-, γ-globulin), creatinine, total bilirubin, urea, uric acid, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase, glucose triacylglycerol and cholesterol. At the stressed stage of regulatory systems the group of bucks showed a reduction of total bilirubin on the background of decrease of ALT, AST and alkaline phosphatase, which can testify to activation of liver function at this stage, and increase in bilirubin, ALT, AST with a simultaneous decrease in alkaline phosphatase, noted at the overstress stage of physiological adaptation regulatory processes, can testify to decrease in the functional activity of hepatocytes.Pregnant rabbits have the most pronounced compensatory reactions in the third stage of the adaptation process: the increase in absolute and relative γ-globulin values indicates the activation of immune factors. An increase in creatinine and urea levels during the gestation period, against a background of developing adaptive changes on the plantar surface of the feet, indicates a decrease in the filtration capacity of the renal tubules.Lactating animals experience an increased physiological load on the liver, which is expressed by an increase in triacylglycerols throughout the adaptation.

scholarly journals COMPARATIVE STUDY OF ALCOHOLIC AND AQUEOUS EXTRACTS OF SYZYGIUM CUMINI ON CARBON TETRACHLORIDE-INDUCED HEPATOTOXICITY IN WISTAR RATS

2016 ◽  
Vol 9 (9) ◽  
pp. 182
Author(s):  
Shweta Sharma ◽  
Mehta Bk
Keyword(s):  
Alkaline Phosphatase ◽  
Carbon Tetrachloride ◽  
Wistar Rats ◽  
Hepatic Injury ◽  
Total Bilirubin ◽  
Ethanolic Extract ◽  
Aqueous Extracts ◽  
Syzygium Cumini ◽  
Standard Drug ◽  
Ethanolic Extracts

ABSTRACTObjective: In this investigation, the comparative hepatoprotective effect of aqueous and ethanolic extracts of Syzygium cumini (AESC and EESC,respectively) was studied on carbon tetrachloride (CCl4)-induced hepatic injury in rats. These findings were also compared with the standardhepatoprotective drug silymarin.Methods: Hepatotoxicity was induced by a single dose of CCl4 to healthy Wistar rats. Standard drug (100 mg/kg) and test extracts (500 mg/kg forboth) were given orally for 10 days; the effects were observed using different biochemical and histological methods.Results: In most of the studied parameters test extracts exhibited significant hepatoprotection, these were comparative to standard. Histologicalanalysis also revealed the protective nature of both the extracts.Conclusion: These results suggest that the SC extracts can ameliorate CCl4 induced hepatic injury. However, its ethanolic extract was found to berelatively less effective than aqueous extract. Indicated, some hydrophilic active compound of SC might work here.Keywords: Hepatoprotective, Carbon tetrachloride, Silymarin, Syzygium cumini, Alkaline phosphatase, Total bilirubin.

scholarly journals Phytochemical Characterization, Hepatoprotective Activity on Alcohol-Induced Toxicity of the Aqueous Extract of Curcuma longa (Zingiberaceae) in Wistar Rats

2021 ◽  
pp. 134-149
Author(s):  
Ameaka Fatima Nkempu ◽  
Tembe Estella ◽  
Tchadji Mayoudom Vanessa Edwige ◽  
Bayaga Herve ◽  
Dobgima John Fonmboh ◽  
...  
Keyword(s):  
Liver Disease ◽  
Secondary Metabolites ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Liver Toxicity ◽  
Curcuma Longa ◽  
Hepatoprotective Activity ◽  
Bioactive Metabolites ◽  
Central Vein ◽  
Phytochemical Screening

Introduction: Liver or hepatic disease refers to different conditions that affect the liver. Chronic alcohol consumption is one of the most frequent causes of liver disease and accounts for about 55% of liver cirrhosis deaths recorded in Cameroon in 2020. Standard accessible treatments focus on end-stage liver disease with safety and efficacy obstacles. We have a research gap in Cameroon to understand the alternative use of natural products as treatment with a long traditional history of safe use. Curcuma longa has long been a source of traditional and modern medicine. It is commonly used in Cameroon as a spice and herbal product with some level of activity against various forms of liver disease. Objective: To phytochemically screen for bioactive metabolites and evaluate the hepatoprotective activity of the aqueous extract of Curcuma longaon alcohol-induced toxicity in Wistar rats. Methods: Phytochemical screening was carried out on the aqueous extract obtainedfrom maceration of plant rhizomes. Three doses (125, 250 and 500mg/Kg) of the plant extract and the reference (Silymarin 50mg/Kg) were administered daily (p.o) to rats 30 min before administration of 40% alcohol (2mL/100g p.o) for 21 days. Biochemical parameters such as ALAT, ASAT, GGT, Bilirubin and Lipid profile were quantified and histological studies of the liverwas carried out using standard procedures. Results: Phytochemical screening of the aqueous extract of C. longa revealed polyphenols such as flavonoids, tannins, quinones, saponins and phlobatanins. The plant showed hepatoprotective activity by decreasing liver toxicity markers such as ASAT, ALAT, GGT and Bilirubin. Histology revealed dose-dependent protection with 500 mg/Kg showing the most cellular integrity, no central vein occlusion and minimal fibrosis. Conclusion: This study indicated the presence of polyphenols like flavonoids and tannins in the aqueous extract of C. longa. The presence of these secondary metabolites in the studied extract justifies its antioxidant and anti-inflammatory properties confirmed by its hepatoprotective effects on alcohol-induced toxicity. This was clearly shown by biochemical and histological parameters. More sensitive and specific methods are required to test for these secondary metabolites in serum.

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