Isolation and Mycelium Growth of Ganoderma lucidum on Manihot Esculent Substrate with Mineral Supplement

2019 ◽  
Vol 6 (2) ◽  
pp. 123-127
Author(s):  
Nguyen Thi Tuyet Nhung ◽  
Keyword(s):  
Ganoderma Lucidum ◽  
Mycelium Growth ◽  
Mineral Supplement

scholarly journals Effect of common foods as supplements for the mycelium growth of Ganoderma lucidum and Pleurotus ostreatus on solid substrates

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0260170
Author(s):  
Eugene Soh ◽  
Nazanin Saeidi ◽  
Alireza Javadian ◽  
Dirk E. Hebel ◽  
Hortense Le Ferrand
Keyword(s):  
Circular Economy ◽  
Pleurotus Ostreatus ◽  
Ganoderma Lucidum ◽  
Fungal Species ◽  
Food Supplements ◽  
Mycelium Growth ◽  
Substrate Composition ◽  
Solid Substrates ◽  
The Many ◽  
Growth Of Fungi

The transition from a linear to a circular economy is urgently needed to mitigate environmental impacts and loss of biodiversity. Among the many potential solutions, the development of entirely natural-based materials derived from waste is promising. One such material is mycelium-bound composites obtained from the growth of fungi onto solid lignocellulosic substrates, which find applications such as insulating foams, textiles, packaging, etc. During growth, the fungus degrades and digests the substrate to create a web-like stiff network called mycelium. The development of the mycelium is influenced by several factors, including the substrate composition. As food waste accounts for nearly 44% of total municipal solid waste, incorporating food in the substrate composition could be a means to increase the nutrients absorbed by the fungus. In this paper, we study the effects of the addition of food supplements on the growth of two fungal species, Ganoderma lucidum and Pleurotus ostreatus. The substrates, the food supplements, and the mycelia are characterized using Fourier-transform infrared spectroscopy, scanning electron microscopy, and optical microscopy. Our results show that addition of barley as a supplement significantly boosts the growth of G. lucidum and P. ostreatus. Using a common food as a nutritious enrichment for the development of mycelium is a simple and straightforward strategy to create waste-based mycelium-bound biocomposites for a large range of applications, on-site, therefore promoting a circular economy.

Resource Utilization Residue of Pleurotus ostreatus

2013 ◽  
Vol 807-809 ◽  
pp. 2048-2053
Author(s):  
Yong Xiong ◽  
Chun Yan Zhao ◽  
Yi Jian Chen ◽  
Wan Song Yue
Keyword(s):  
Pleurotus Ostreatus ◽  
Ganoderma Lucidum ◽  
Water Extract ◽  
Ethanol Extract ◽  
Flammulina Velutipes ◽  
Edible Fungi ◽  
Mycelium Growth ◽  
Extract Concentration ◽  
Ethanol Extracts ◽  
Better Than

The allelopathy of spent substrate extracts including water extract and ethanol extract ofPleurotus ostreatuson the mycelium growth of six edible fungi, i.e.Flammulina velutipes,Ganoderma lucidum Karst,Pleurotus ostreatusandCordyceps (three species), were investigated using Petri dishes approach. The results indicated that the spent substrate extracts have different effects on their mycelium growth. The mycelium ofFlammulina velutipesgrows better than control check with increasing water extract concentration. The mycelia ofGanoderma lucidum Karstgrow first promotion after inhibition with increasing water extract concentration. The mycelia ofFlammulina velutipes, Ganoderma lucidum Karst, Pleurotus ostreatusare promoted by ethanol extracts. The mycelia of Cordyceps (three species) grow first promotion after inhibition with ethanol extracts. The results can provide reference values for rational utilization of the spent mushroom substrate.

scholarly journals Mycelium Growth and Biological Efficiency of Ganoderma lucidum on Substrate Supplemented with Different Organic Additives

2015 ◽  
Vol 60 (2) ◽  
pp. 303-308
Author(s):  
Jolanta Lisiecka ◽  
Jerzy Rogalski ◽  
Krzysztof Sobieralski ◽  
Marek Siwulski ◽  
Sławomir Sokół ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Biological Efficiency ◽  
Organic Additives ◽  
Mycelium Growth

scholarly journals Heat Stress Modulates Mycelium Growth, Heat Shock Protein Expression, Ganoderic Acid Biosynthesis, and Hyphal Branching of Ganoderma lucidum via Cytosolic Ca2+

2016 ◽  
Vol 82 (14) ◽  
pp. 4112-4125 ◽  
Author(s):  
Xue Zhang ◽  
Ang Ren ◽  
Meng-Jiao Li ◽  
Peng-Fei Cao ◽  
Tian-Xi Chen ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Heat Stress ◽  
Heat Shock ◽  
Ganoderma Lucidum ◽  
Potential Model ◽  
Acid Biosynthesis ◽  
Ganoderic Acid ◽  
Mycelium Growth ◽  
Hyphal Branching ◽  
Content Type

ABSTRACTHeat stress (HS) influences the growth and development of organisms. Thus, a comprehensive understanding of how organisms sense HS and respond to it is required.Ganoderma lucidum, a higher basidiomycete with bioactive secondary metabolites, has become a potential model system due to the complete sequencing of its genome, transgenic systems, and reliable reverse genetic tools. In this study, we found that HS inhibited mycelium growth, reduced hyphal branching, and induced the accumulation of ganoderic acid biosynthesis and heat shock proteins (HSPs) inG. lucidum. Our data showed that HS induced a significant increase in cytosolic Ca2+concentration. Further evidence showed that Ca2+might be a factor in the HS-mediated regulation of hyphal branching, ganoderic acid (GA) biosynthesis, and the accumulation of HSPs. Our results further showed that the calcium-permeable channel gene (cch)-silenced and phosphoinositide-specific phospholipase gene (plc)-silenced strains reduced the HS-induced increase in HSP expression compared with that observed for the wild type (WT). This study demonstrates that cytosolic Ca2+participates in heat shock signal transduction and regulates downstream events in filamentous fungi.IMPORTANCEGanoderma lucidum, a higher basidiomycete with bioactive secondary metabolites, has become a potential model system for evaluating how environmental factors regulate the development and secondary metabolism of basidiomycetes. Heat stress (HS) is an important environmental challenge. In this study, we found that HS inhibited mycelium growth, reduced hyphal branching, and induced HSP expression and ganoderic acid biosynthesis inG. lucidum. Further evidence showed that Ca2+might be a factor in the HS-mediated regulation of hyphal branching, GA biosynthesis, and the accumulation of HSPs. This study demonstrates that cytosolic Ca2+participates in heat shock signal transduction and regulates downstream events in filamentous fungi. Our research offers a new way to understand the mechanism underlying the physiological and metabolic responses to other environmental factors inG. lucidum. This research may also provide the basis for heat shock signal transduction studies of other fungi.

Efficacy and Safety of Regular Vitamin and Mineral Supplement Use in France: Results from the ECCA Study

2005 ◽  
Vol 75 (3) ◽  
pp. 201-209 ◽  
Author(s):  
Touvier ◽  
Boutron-Ruault ◽  
Volatier ◽  
Martin
Keyword(s):  
Pantothenic Acid ◽  
Assessment Tools ◽  
Mineral Supplement ◽  
Mineral Supplements ◽  
Supplement Use ◽  
Micronutrient Intake ◽  
Frequency Of Use ◽  
Retail Outlets ◽  
Annual Frequency ◽  
Excessive Intake

This study investigated the prevalence of inadequate micronutrient intake and the proportion of subjects who exceed Tolerable Upper Intake Levels a) with food only, and b) with food+supplements, in a population of French regular supplement users (n = 259). Assessment tools were seven-day records for supplements, three-day records for food intake, and a questionnaire about supplement use. Most subjects were recruited in retail outlets that sold supplements. They were recent users of vitamin/mineral supplements, aged over 15 years, and normo-energy reporters. The prevalence (%) of inadequate intake decreased with the inclusion of mean annual supplements, from 68.0 to 54.8 for magnesium, 55.9 to 40.7 for vitamin C, 53.4 to 43.9 for folic acid, 37.5 to 27.5 for iron, and 40.1 to 29.7 for pantothenic acid. Few subjects exceeded upper intake levels when mean annual intake of supplements was considered. When supplement consumption was considered during the studied week only, the proportion of subjects who were in excess of the upper intake levels was higher (maximum: 9.6% for magnesium). Supplement use brought a nutritional benefit for some targeted nutrients. It was not associated with excessive intake in this study, but could become hazardous if the annual frequency of use were to increase.

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