Resource Utilization Residue of Pleurotus ostreatus

2013 ◽  
Vol 807-809 ◽  
pp. 2048-2053
Author(s):  
Yong Xiong ◽  
Chun Yan Zhao ◽  
Yi Jian Chen ◽  
Wan Song Yue
Keyword(s):  
Pleurotus Ostreatus ◽  
Ganoderma Lucidum ◽  
Water Extract ◽  
Ethanol Extract ◽  
Flammulina Velutipes ◽  
Edible Fungi ◽  
Mycelium Growth ◽  
Extract Concentration ◽  
Ethanol Extracts ◽  
Better Than

The allelopathy of spent substrate extracts including water extract and ethanol extract ofPleurotus ostreatuson the mycelium growth of six edible fungi, i.e.Flammulina velutipes,Ganoderma lucidum Karst,Pleurotus ostreatusandCordyceps (three species), were investigated using Petri dishes approach. The results indicated that the spent substrate extracts have different effects on their mycelium growth. The mycelium ofFlammulina velutipesgrows better than control check with increasing water extract concentration. The mycelia ofGanoderma lucidum Karstgrow first promotion after inhibition with increasing water extract concentration. The mycelia ofFlammulina velutipes, Ganoderma lucidum Karst, Pleurotus ostreatusare promoted by ethanol extracts. The mycelia of Cordyceps (three species) grow first promotion after inhibition with ethanol extracts. The results can provide reference values for rational utilization of the spent mushroom substrate.

scholarly journals Antibacterial Potency of Pleurotus ostreatus Extract From Fruiting Body and its Solid Substrate on Staphyllococcus aureus

2020 ◽  
Vol 15 (2) ◽  
pp. 142-147
Author(s):  
I. Badarina ◽  
D. Evvyernie ◽  
E. N. Herliyana ◽  
L. K. Darusman ◽  
T. Toharmat
Keyword(s):  
Fruiting Body ◽  
Pleurotus Ostreatus ◽  
Ganoderma Lucidum ◽  
Ethanol Extract ◽  
Solid Substrate ◽  
Ethanol Solution ◽  
Coffee Husk ◽  
Tetracycline Antibiotic ◽  
Significant Difference ◽  
Ethanol Extracts

The objective of this research was to evaluate the antibacterial potency of ethanol extract from fruiting body of  Pleurotus ostreatus and Its solid substrate made from coffee husk and sawdust on the growth of Staphylococcus aureus ATCC 25922.  The extract of Ganoderma lucidum fruiting body and Tetracycline antibiotic paper disk 30µg/disk were used as control.  The samples were extracted by using maceration method in  30% ethanol solution.  The extracts were diluted with sterile distilled water to concentration 500, 1000, and 5000ppm.  The result showed that the ethanol extracts from fruiting body  of  Pleurotus ostreatus and Ganoderma lucidum and the extracts of coffee husk and sawdust substrate fermented by Pleurotus ostreatus could inhibit the growth of bacteria for all the concentration. There was a significant difference in diameter of cleared zones between  Tetracycline antibiotic disc 30?g  and the ethanol extracts of the samples (p<0.01).  The diameter of cleared zones among the sample extracts and each dilution concentrations were not a significant difference (p>0.05).  Tetracycline was sensitive to S.aureus ATCC25922, while all the extracts were resistant.   This study confirmed that there were the antibacterial potency of mushroom extracts from the fruiting body and also its solid substrates.

Inhibition of Aflatoxin-Producing Fungi by Welsh Onion Extracts

1999 ◽  
Vol 62 (4) ◽  
pp. 414-417 ◽  
Author(s):  
J. J. FAN ◽  
J. H. CHEN
Keyword(s):  
Aspergillus Flavus ◽  
Mycelial Growth ◽  
Ethanol Extract ◽  
Aflatoxin Production ◽  
Inhibitory Effects ◽  
Welsh Onion ◽  
Extract Concentration ◽  
Ph Values ◽  
Ethanol Extracts ◽  
Yeast Extract Sucrose

Welsh onion ethanol extracts were tested for their inhibitory activity against the growth and aflatoxin production of Aspergillus flavus and A. parasiticus. The survival of spores of A. flavus and A. parasiticus depended on both the extract concentration and the exposure time of the spores to the Welsh onion extracts. The mycelial growth of two tested fungi cultured on yeast extract–sucrose broth was completely inhibited in the presence of the Welsh onion ethanol extract at a concentration of 10 mg/ml during 30 days of incubation at 25°C. The extracts added to the cultures also inhibited aflatoxin production at a concentration of 10 mg/ml or permitted only a small amount of aflatoxin production with extract concentration of 5 mg/ml after 2 weeks of incubation. Welsh onion ethanol extracts showed more pronounced inhibitory effects against the two tested aflatoxin-producing fungi than did the same added levels of the preservatives sorbate and propionate at pH values near 6.5.

scholarly journals Uji Aktivitas Antioksidan Ekstrak Air dan Ekstrak Etanol Daun Kelor (Moringa Oleifera LAM)

2017 ◽  
Vol 6 (2) ◽  
pp. 125
Author(s):  
Rizkayanti Rizkayanti ◽  
Anang Wahid M. Diah ◽  
Minarni Rama Jura
Keyword(s):  
Vitamin C ◽  
Moringa Oleifera ◽  
Water Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Ic50 Value ◽  
Antioxidant Potency ◽  
Ethanol Extracts ◽  
Moringa Oleifera Lam

Moringa (moringa oleifera Lam) leaves contains many molecules as inhibitors for free radicals such as phenolic compounds (phenolic acids, flavonoids, quinones, coumarins, lignans, stilbenes, tannins), nitrogen compounds (alkaloids, amines, betalain), vitamins, terpenoids (including carotenoids), and several other endogenous metabolites as antioxidants. This study aimed to determine the antioxidant potency of water and ethanol extracts of moringa (moringa oleifera Lam) leave obtained by maceration and dekok. The concentration of free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) was analyzed using UV-Vis spectrophotometer after addition of various concentrations of Moringa leaves extracts. Various concentrations of moringa leave extracts used were 20 ppm, 40 ppm, 60 ppm and 80 ppm. Vitamin C solutions as the positive control were prepared on similar various concentrations. The negative control was prepared using DPPH solutions dissolved in absolute ethanol. The results indicated that the ethanol extract of moringa leaves prepared by maceration method showed the antioxidant potency with an IC50 value of 22.1818 ppm, but the IC50 value of water extract of moringa leaves prepared by dekok was 57.5439 ppm. While, the IC50 value of Vitamin C was 8.8084 ppm. Based on the IC50 data it can be concluded that Vitamin C is a stronger antioxidant than moringa leaves extracts.

scholarly journals PHYTOCHEMICAL SCREENING AND ANTI-INFLAMMATORY EFFECT TEST OF ETHANOL EXTRACT OF TURMERIC RHIZOME (Curcuma domestica Val.)TOWARD MALE WHITE MOUSE (Rattus norvegicus)

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Rulia Meilina
Keyword(s):  
Ethanol Extract ◽  
White Mouse ◽  
Phytochemical Screening ◽  
Suspension Control ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Group B ◽  
Group D ◽  
Better Than ◽  
Inflammatory Effect

Anti-inflammatory effect of ethanol rhizome of turmeric was conducted toward white mouse which is induced the carrageenan solution (lambda carrageenan) 1 %, research was conducted by dividing the animals into 5 groups.Group A was given CMC 0,5%, group B was given Indometacin with dose 100 mg/kgBB, group C was given  ethanol rhizome extract of turmeric with dose 400 mg/kgBB, group D was given ethanol rhizome extract of turmeric with dose 500 mg/kgBB, group E was given ethanol rhizome extract of turmeric with dose 600 mg/kgBB. Result of phytochemical screening toward powder and ethanol extract of rhizome of turmeric shows that chemical compounds which are involved are alkaloids, flavonoids, and aetheric oil. In the other hand, result of phytochemical screening toward ethanol extract of turmeric’s rhizome are alkaloids,flavonoids,anthraquinones, glycosides. Result of anti-inflammatory test shows that there is the real difference among groups of mouse which were given CMC suspension (control), suspension of ethanol extracts of turmeric’s rhizome with dose 400 mg/kg BB, 500 mg/kg BB, 600 mg/kg BB (material test) and suspension of indomethacin with dose 100 mg/kg BB (positive comparison). Suspension of ethanol extract of turmeric’s rhizome with dose 600 mg/kg BB shows that anti-inflammatory capability is better than dose 400 mg/kg BB and 500 mg/kg BB, and suspension of ethanol extract of turmeric’s rhizome dose 600 mg/kg BB shows the anti-inflammatory effect which is not really different with suspension of indomethacin with dose 100 mg/kg BB, it is showed by  ANOVA statistic analysis among those comparisons toward indomethacin suspension as positive comparison.Keywords:Anti-inflammatory, Indomethacin, Turmeric Rhizome

scholarly journals Effect of common foods as supplements for the mycelium growth of Ganoderma lucidum and Pleurotus ostreatus on solid substrates

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0260170
Author(s):  
Eugene Soh ◽  
Nazanin Saeidi ◽  
Alireza Javadian ◽  
Dirk E. Hebel ◽  
Hortense Le Ferrand
Keyword(s):  
Circular Economy ◽  
Pleurotus Ostreatus ◽  
Ganoderma Lucidum ◽  
Fungal Species ◽  
Food Supplements ◽  
Mycelium Growth ◽  
Substrate Composition ◽  
Solid Substrates ◽  
The Many ◽  
Growth Of Fungi

The transition from a linear to a circular economy is urgently needed to mitigate environmental impacts and loss of biodiversity. Among the many potential solutions, the development of entirely natural-based materials derived from waste is promising. One such material is mycelium-bound composites obtained from the growth of fungi onto solid lignocellulosic substrates, which find applications such as insulating foams, textiles, packaging, etc. During growth, the fungus degrades and digests the substrate to create a web-like stiff network called mycelium. The development of the mycelium is influenced by several factors, including the substrate composition. As food waste accounts for nearly 44% of total municipal solid waste, incorporating food in the substrate composition could be a means to increase the nutrients absorbed by the fungus. In this paper, we study the effects of the addition of food supplements on the growth of two fungal species, Ganoderma lucidum and Pleurotus ostreatus. The substrates, the food supplements, and the mycelia are characterized using Fourier-transform infrared spectroscopy, scanning electron microscopy, and optical microscopy. Our results show that addition of barley as a supplement significantly boosts the growth of G. lucidum and P. ostreatus. Using a common food as a nutritious enrichment for the development of mycelium is a simple and straightforward strategy to create waste-based mycelium-bound biocomposites for a large range of applications, on-site, therefore promoting a circular economy.

scholarly journals AKTIVITAS ANTIOKSIDAN DAN IDENTIFIKASI SENYAWA EKSTRAK JAMUR LINGZHI (Ganoderma lucidum) DENGAN LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY (LC-MS)

EKOLOGIA ◽  
2019 ◽  
Vol 19 (2) ◽  
pp. 65-72
Author(s):  
Farida Nuraeni ◽  
Septi Bernadetha Br Sembiring
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Time Variation ◽  
Ganoderma Lucidum ◽  
Water Extract ◽  
Ethanol Extract ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Dpph Method

Lingzhi mushroom is widely used as an alternative treatment to reduce blood pressure and blood sugar levels, to maximize the potential of the Lingzhi fungus antioxidant tests are carried out. This study aims to determine the potential antioxidant activity of water extract and 70% ethanol extract of Lingzhi mushroom with maceration extraction time variation and identification of compounds with Liquid Chromatography Mass Spectrometry (LC-MS). This research begins with the determination of fresh lingzhi mushrooms then made simplicia and extracted by maceration with time variation with soaking time of 24 hours, 48 hours and 72 hours each with 2 solvents namely water and 70% ethanol. The extract was tested by phytochemistry followed by testing the antioxidant activity of Lingzhi mushroom extract (ganoderma lucidum) by DPPH method. Then the compounds were identified by Liquid Chromatography-Mass Spectrometry (LC-MS). Based on the results of research that maceration for 1 hour (1 day) Lingzhi mushroom with 70% ethanol extraction has the potential as an antioxidant with an IC50 value of 94.83 ppm. The results of identification with LC-MS in 70% ethanol extract as a compound that has potential as an antioxidant are Bisphenol M compounds, and 1- - [[2- (3,4-Dimethoxyphenyl) amino]} -3-methyl-2-octylpyrido [1 , 2-a] benzimidazole-4-carbonitrile.

scholarly journals Chromatographic Analysis (LC-MS and GC-MS), Antioxidant Activity, Total Phenol and Total Flavonoid Determination of Ononis natrix L. Grown in Jordan

2021 ◽  
Vol 16 (1) ◽  
pp. 31-39
Keyword(s):  
Solid Phase ◽  
Water Extract ◽  
Ethanol Extract ◽  
Total Phenol ◽  
Aroma Profile ◽  
Sesquiterpene Hydrocarbons ◽  
Leaf Oil ◽  
Terpenoid Aldehydes ◽  
Ethanol Extracts

The purpose of the present study was to identify, by gas chromatography (GC) and GC-mass spectrometry (GC-MS), the components of the spontaneously emitted volatile organic compounds (VOCs), obtained by Solid-Phase-Micro-Extraction (SPME) and that of the hydrodistilled oil of the fresh flowers and leaves of Ononis natrix, as well as to compare them. The hydrodistilled leaf oil was rich in non-terpenoid aldehydes, whereas its aroma profile contained mainly sesquiterpene hydrocarbons with α-copaene and germacrene D as their major components. The hydrodistilled oil of the fresh flowers, however, revealed nearly equal amounts of terpenoid and non-terpenoid compounds; 51.00% and 46.54%, respectively. The aroma profile of the fresh flowers was dominated by monoterpene hydrocarbons with α-pinene (42.96%) and α-thujene (20.17%) as the predomi¬nant two monoterpenes. Based on the high total phenol and flavonoid contents of the water and ethanol extracts, LC-MS analysis was carried out to identify the major compounds from each sample. From the water extract, eleven compounds were identified, whereas the ethanol extract contained eight, whereby luteolin (from the water extract) and apigenin (from the ethanol one) were named as the major flavonoids, respectively.

scholarly journals USE OF ZPT AND PLANTING MEDIA ON THE GROWTH OF MYCELIUM OYSTER MUSHROOM (Pleurotus ostreatus)

2020 ◽  
Vol 7 (1) ◽  
pp. 2125-2131
Author(s):  
Joshua H.L. Tobing ◽  
Donn Ricky ◽  
Meyria K Situmeang
Keyword(s):  
Acetic Acid ◽  
Pleurotus Ostreatus ◽  
Water Extract ◽  
Boiling Water ◽  
Oyster Mushroom ◽  
Naphthalene Acetic Acid ◽  
Duncan Multiple Range Test ◽  
Green Beans ◽  
Mycelium Growth ◽  
Range Test

White Oyster mushroom (Pleurotus ostreatus) is an alternative food for the society because of its high nutrients content. This study uses of ZPT and media to see the grow of the mycelium white oyster mushroom. Media used are the boiling water extract of nuts (green beans, soy bean, peanuts, beans of string bean, and beans of nut snaps) and ZPT (Naphthalene Acetic Acid and Kinetin). Anova was used to analyze the data at a significant level of α = 0.05. The results shows that: (1) There’s a significant effect of ZPT and Non-ZPT on the mycelium growth with p=0.000, Duncan Multiple Range Test (DMRT) shows that Kinetin is highest contribution to the significancy of Anova; (2) planting media used in the study shows a significant differences on the mycelium growth with p=0039, Duncan Multiple Range Test shows that beans of long bean and soy bean are the highest contribution to the significancy of Anova; (3) time/days of observation  done in the study shows a significant differences on the mycelium growth with p=0000, Duncan Multiple Range Test shows that T6 or day 14 of observation shows the highest contribution to the significancy of Anova; (4) the interaction of ZPT, Non-ZPT and planting media show a significant differences on the mycelium growth p=0000; (5) the interaction of ZPT, Non-ZPT and time show a significant differences on the mycelium growth with p=0000; (6) the interaction of media and planting time show a significant differences on the mycelium growth with p=0000; and (7) the interaction of ZPT, Non-ZPT and planting media and time do not affect significantly the mycelium growth with  p=0053.

scholarly journals The effect of hot water and ethanol extract of Nigella sativa in immune system of Albino Mice

2009 ◽  
Vol 6 (2) ◽  
pp. 235-243
Author(s):  
Baghdad Science Journal
Keyword(s):  
Nigella Sativa ◽  
Water Extract ◽  
Ethanol Extract ◽  
Hot Water ◽  
Delayed Type Hypersensitivity ◽  
Differential Count ◽  
Alcoholic Extract ◽  
Albino Mice ◽  
Ethanol Extracts ◽  
High Level

The result showed that hot water and ethanol extracts of Nigella sativa contain alkaloids ,saponins, flavonoids,tannins,glycosides,terpins and steroids. Albino mice were administered orally with 0.1 of the extract at dose of 100 mg/kg, body weight the results showed high level of white blood cell ,total and differential count of WBC,phagocytosis index, mitotic index, Arthus and delayed type hypersensitivity. The result, also showed high level of hemoglobin (Hb) and the packed cell volume (PCV) the alcoholic extract has been found more efficient than hot water extract on mice.

scholarly journals Study on Cardiotoxicity and Mechanism of “Fuzi” Extracts Based on Metabonomics

2018 ◽  
Vol 19 (11) ◽  
pp. 3506 ◽  
Author(s):  
Guangyao Huang ◽  
Liang Yang ◽  
Wei Zhou ◽  
Xianglin Tang ◽  
Yuguang Wang ◽  
...  
Keyword(s):  
Transforming Growth Factor ◽  
Mammalian Target Of Rapamycin ◽  
Water Extract ◽  
Ethanol Extract ◽  
Transforming Growth Factor Β1 ◽  
Heart Tissue ◽  
Serum Lactate ◽  
Serum Lactate Dehydrogenase ◽  
Ethanol Extracts ◽  
Tgf Β1

To investigate the toxicity of water and ethanol “Fuzi” (FZ) extracts and to explore the toxicity mechanism in rats. Water and ethanol extracts were prepared. Three groups of rats received the water extract, ethanol extract, or water by oral gavage for seven days. Pathological section staining of heart tissue. Colorimetric analysis was used to determine serum lactate dehydrogenase. The metabolic expression of small molecules in rats was measured by a metabolomics method. Western blotting was used to detect the expression of phosphoinositide 3-kinase (PI3K), protein kinase B (Akt), mammalian target of rapamycin (mTOR), transforming growth factor-β1 (TGF-β1), and caspase-3. Immunohistochemistry was used to detect the expression of CTnI, mTOR, and TGF-β1. The water and ethanol FZ extracts exert cardiotoxic effects via activating the PI3K/Akt/mTOR signaling pathway to induce cardiomyocyte apoptosis.

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