scholarly journals Effect of common foods as supplements for the mycelium growth of Ganoderma lucidum and Pleurotus ostreatus on solid substrates

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0260170
Author(s):  
Eugene Soh ◽  
Nazanin Saeidi ◽  
Alireza Javadian ◽  
Dirk E. Hebel ◽  
Hortense Le Ferrand
Keyword(s):  
Circular Economy ◽  
Pleurotus Ostreatus ◽  
Ganoderma Lucidum ◽  
Fungal Species ◽  
Food Supplements ◽  
Mycelium Growth ◽  
Substrate Composition ◽  
Solid Substrates ◽  
The Many ◽  
Growth Of Fungi

The transition from a linear to a circular economy is urgently needed to mitigate environmental impacts and loss of biodiversity. Among the many potential solutions, the development of entirely natural-based materials derived from waste is promising. One such material is mycelium-bound composites obtained from the growth of fungi onto solid lignocellulosic substrates, which find applications such as insulating foams, textiles, packaging, etc. During growth, the fungus degrades and digests the substrate to create a web-like stiff network called mycelium. The development of the mycelium is influenced by several factors, including the substrate composition. As food waste accounts for nearly 44% of total municipal solid waste, incorporating food in the substrate composition could be a means to increase the nutrients absorbed by the fungus. In this paper, we study the effects of the addition of food supplements on the growth of two fungal species, Ganoderma lucidum and Pleurotus ostreatus. The substrates, the food supplements, and the mycelia are characterized using Fourier-transform infrared spectroscopy, scanning electron microscopy, and optical microscopy. Our results show that addition of barley as a supplement significantly boosts the growth of G. lucidum and P. ostreatus. Using a common food as a nutritious enrichment for the development of mycelium is a simple and straightforward strategy to create waste-based mycelium-bound biocomposites for a large range of applications, on-site, therefore promoting a circular economy.

Resource Utilization Residue of Pleurotus ostreatus

2013 ◽  
Vol 807-809 ◽  
pp. 2048-2053
Author(s):  
Yong Xiong ◽  
Chun Yan Zhao ◽  
Yi Jian Chen ◽  
Wan Song Yue
Keyword(s):  
Pleurotus Ostreatus ◽  
Ganoderma Lucidum ◽  
Water Extract ◽  
Ethanol Extract ◽  
Flammulina Velutipes ◽  
Edible Fungi ◽  
Mycelium Growth ◽  
Extract Concentration ◽  
Ethanol Extracts ◽  
Better Than

The allelopathy of spent substrate extracts including water extract and ethanol extract ofPleurotus ostreatuson the mycelium growth of six edible fungi, i.e.Flammulina velutipes,Ganoderma lucidum Karst,Pleurotus ostreatusandCordyceps (three species), were investigated using Petri dishes approach. The results indicated that the spent substrate extracts have different effects on their mycelium growth. The mycelium ofFlammulina velutipesgrows better than control check with increasing water extract concentration. The mycelia ofGanoderma lucidum Karstgrow first promotion after inhibition with increasing water extract concentration. The mycelia ofFlammulina velutipes, Ganoderma lucidum Karst, Pleurotus ostreatusare promoted by ethanol extracts. The mycelia of Cordyceps (three species) grow first promotion after inhibition with ethanol extracts. The results can provide reference values for rational utilization of the spent mushroom substrate.

scholarly journals Screening of cellulose- and lignin-degrading fungi for improving nutritive quality of ruminant feed

2018 ◽  
Vol 197 ◽  
pp. 06001
Author(s):  
Muhammad Fauzi ◽  
Nisa Rachmania Mubarik ◽  
Anuraga Jayanegara
Keyword(s):  
Rice Straw ◽  
Phanerochaete Chrysosporium ◽  
Pleurotus Ostreatus ◽  
Manganese Peroxidase ◽  
Ganoderma Lucidum ◽  
Fungal Species ◽  
Potato Dextrose Broth ◽  
Nutritive Quality ◽  
Ruminant Feed

This experiment aimed to screen some cellulose- and lignin-degrading fungal species for improving nutritive quality of ruminant feed. Fungal species used were Ganoderma lucidum, Pleurotus ostreatus, Phanerochaete chrysosporium, and Trametes visicolor. These fungal species were grown on Potato Dextrose Broth (PDB) medium that added with 0.5 g substrate, i.e. either rice straw, rice bran or carboxymethyl cellulose for 12 days at room temperature. Harvesting was performed in order to separate fungal mycelium and supernatant that contained crude cellulase enzyme. Among the fungi observed, Ganoderma lucidum had the highest cellulase activity, i.e. 4.02 U/mg. Cellulase activities of Pleurotus ostreatus, Phanerochaete chrysosporium, and Trametes visicolor were 1.53, 1.08, and 0.13 U/mg, respectively. Ganoderma lucidum was further investigated for its ligninolytic enzyme activity, i.e. laccase and manganese peroxidase. It was observed that the fungi had laccase and manganese peroxidase activities of 368.95 and 33.89 U/mL, respectively. Crude enzymes of Ganoderma lucidum that contain cellulase, laccase and manganese peroxidase were further used to increase nutritive quality of rice straw and subjected to in vitro incubation with rumen fluid of cattle.

scholarly journals Making Resilient Decisions for Sustainable Circularity of Fashion

2021 ◽  
Author(s):  
Celinda Palm ◽  
Sarah E. Cornell ◽  
Tiina Häyhä
Keyword(s):  
Value Chain ◽  
Circular Economy ◽  
Ecological Impacts ◽  
Ecological System ◽  
Fashion Industry ◽  
State Response ◽  
Social Ecological ◽  
Industry Value Chain ◽  
The Many ◽  
Sustainability Challenges

AbstractThe fashion and textiles industry, and policymakers at all levels, are showing an increased interest in the concept of circular economy as a way to decrease business risks and negative environmental impacts. However, focus is placed mainly on the material ‘stuff’ of textile fashion and its biophysical harms. The current material focus has several shortcomings, because fashion is a social-ecological system and cannot be understood merely by addressing its environmental dimensions. In this paper, we rethink the fashion system from a critical social-ecological perspective. The driver-state-response framework shows social drivers and ecological impacts as an adaptive social-ecological system, exposing how these interacting aspects need to be addressed for sustainable and resilient implementation of circular economy. We show how current responses to global sustainability challenges have so far fallen short. Our overall aim is to expand possibilities for reframing responses that better reflect the complex links between the global fashion system, culture and creativity and the dynamics of the living planet. We argue that reducing planetary pressure from the global fashion and textiles industry requires greater recognition of the system’s social drivers with more emphasis on the many cross-scale links between social and ecological dimensions. Resilient decisions aiming for sustainable circularity of the fashion industry must therefore pay attention to social activities beyond the industry value chain, not just material flows within it.

scholarly journals Pengaruh penambahan serbuk jagung pada komposisi media tanam terhadap pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus)

2021 ◽  
Vol 10 (2) ◽  
pp. 158-170
Author(s):  
Muhaeming Muhaeming ◽  
Jamilah Jamilah ◽  
Zulkarnaim Zulkarnaim
Keyword(s):  
Experimental Study ◽  
Analysis Of Variance ◽  
Pleurotus Ostreatus ◽  
Medium Composition ◽  
Oyster Mushroom ◽  
Control Treatment ◽  
Mycelium Growth ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Penambahan nutrisi perlu dilakukan untuk meningkatkan produktivitas pertumbuhan jamur tiram putih (Pleurotus ostreatus). Salah satu bahan yang dapat digunakan adalah serbuk jagung. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan serbuk jagung pada komposisi media tanam terhadap pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus) dan mengetahui konsentrasi serbuk jagung yang paling baik digunakan pada pertumbuhan  miselium bibit F1 jamur tiram putih (Pleurotus ostreatus). Penelitian ini mengunakan Rancangan Acak Lengkap (RAL) 4 perlakuan dan 3 ulangan sehingga diperoleh 12 satuan percobaan; P0 (perlakuan kontrol), P1 (25%), P2 (50%), dan P3 (100%). Variabel yang diukur adalah persentase pertumbuhan miselium yang diamati pada hari ke-6, 12 dan 18 dan waktu penyebaran miselium Hari Setelah Inokulasi (HSI). Data hasil penelitian dianalisis menggunakan analysis of variance (ANOVA) dengan uji lanjut menggunakan uji Beda Nyata Terkecil (BNT). Hasil penelitian menunjukkan bahwa ada pengaruh pada pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus) dengan penambahan serbuk jagung pada komposisi media tanam. Selain Itu, konsentrasi serbuk jagung yang memberikan hasil yang paling baik terhadap pertumbuhan miselium adalah P2 dengan konsentrasi 50%. Kata kunci : miselium F1; Pleurotus ostreatus; serbuk jagung  ABSTRACTAdditional nutrition needs to be done to increase the productivity of oyster mushroom (Pleurotus ostreatus) growth. One of the materials that can be used is corn powder. This study aimed to determine the effect of corn powder on the planting medium composition towards mycelium growth of oyster mushroom F1 seeds (Pleurotus ostreatus) and to find the best concentration of corn powder to grow mycelium seeds F1 oyster mushroom (Pleurotus ostreatus). This research was an experimental study using a Completely Randomized Design (CRD) with 4 treatments namely; P0 (control treatment), P1 (25%), P2 (50%), and P3 (100%), and 3 repetitions so that obtained 12 experimental units. The variables measured were the mycelium growth percentage on days 6, 12, and 18, and the mycelium widespread time after the day of inoculation. The data were analyzed using ANOVA then continued on the Least Significant Difference (LSD) test. The results showed that the addition of corn powder on oyster mushroom growing media affected mycelium growth of the oyster mushroom F1 seeds (Pleurotus ostreatus). It also found that the P2 with a 50% concentration of corn powder gave the widest mycelium growth.  Keywords: corn powder; mycelium; Pleurotus ostreatus

scholarly journals Mycoflora of sawn timbers in Benin City

2014 ◽  
Vol 23 (2) ◽  
pp. 89-95
Author(s):  
J. A. Okhuoya ◽  
S. O. Itaman
Keyword(s):  
Moisture Content ◽  
Pleurotus Ostreatus ◽  
Fungal Species ◽  
High Moisture Content ◽  
High Moisture ◽  
Benin City ◽  
The Poor ◽  
High Incidence

In a fungal suryey of sawn timbers in scattared localities in Benin City, fungal species isolated were mainly members of <i>Hyphomycetes</i>, with few <i>Ascomycetes</i> and<i> Basidiomycetes</i>. Cellulolytic abilities of isolates were determined and found to be highest in a basidiomycete, <i>Pleurotus ostreatus</i>. The high incidence of these isolates was traced to the poor ventillation in the shades where the timbers arę sold and the high moisture content of timber before display for sale.

scholarly journals Microbial Degradation of Different Hydrocarbon Fuels with Mycoremediation of Volatiles

2020 ◽  
Vol 8 (2) ◽  
pp. 163 ◽  
Author(s):  
Agota Horel ◽  
Silke Schiewer
Keyword(s):  
Fungal Species ◽  
Bacterial Degradation ◽  
Contaminant Removal ◽  
Total Hydrocarbon ◽  
Diesel Fuels ◽  
Naturally Occurring ◽  
Surface Contaminants ◽  
Favorable Conditions ◽  
Hydrocarbon Substrates ◽  
Growth Of Fungi

Naturally occurring microorganisms in soil matrices play a significant role in overall hydrocarbon contaminant removal. Bacterial and fungal degradation processes are major contributors to aerobic remediation of surface contaminants. This study investigated degradation of conventional diesel, heating diesel fuel, synthetic diesel (Syntroleum), fish biodiesel and a 20% biodiesel/diesel blend by naturally present microbial communities in laboratory microcosms under favorable environmental conditions. Visible fungal remediation was observed with Syntroleum and fish biodiesel contaminated samples, which also showed the highest total hydrocarbon mineralization (>48%) during the first 28 days of the experiment. Heating diesel and conventional diesel fuels showed the lowest total hydrocarbon mineralization with 18–23% under favorable conditions. In concurrent experiments with growth of fungi suspended on a grid in the air space above a specific fuel with little or no soil, fungi were able to survive and grow solely on volatile hydrocarbon compounds as a carbon source. These setups involved negligible bacterial degradation for all five investigated fuel types. Fungal species able to grow on specific hydrocarbon substrates were identified as belonging to the genera of Giberella, Mortierella, Fusarium, Trichoderma, and Penicillium.

scholarly journals Effectiveness of environmentally safe food additives and food supplements in an in vitro growth inhibition of significant Fusarium, Aspergillus and Penicillium species

2018 ◽  
Vol 54 (No. 3) ◽  
pp. 163-173
Author(s):  
Žabka Martin ◽  
Pavela Roman
Keyword(s):  
Food Additives ◽  
Fungal Species ◽  
Food Supplements ◽  
Antifungal Compounds ◽  
Penicillium Species ◽  
The World ◽  
Environmentally Safe ◽  
Safe Food ◽  
The Eu

We tested 38 legislatively recognised substances such as food additives and supplements for antifungal efficacy, with the aim of providing an alternative to synthetic conventional fungicides. These compounds were tested against 9 significant pathogenic fungal species belonging to the significant genera Fusarium, Penicillium, and Aspergillus. Of these compounds, 6 are proposed as potential candidates to provide a complementary alternative to conventional fungicides. Natamycin provided extreme efficacy expressed as MIC50 (5–31 µg/ml), followed by BHA and then BHT, CaNa2EDTA, PABA, and chitosan expressed as MIC50 (0.7–1.9 mg/ml). Safety and antifungal activity were discussed in terms of the mode of action and molecular structure, as well as in terms of potential practical use and legislative requirements for the introduction into practice. We presume that food additives and food supplements are definitely a great source of antifungal compounds. In developed areas of the world (e.g. in the EU), they could represent legislatively recognised compounds, so-called basic substances.

scholarly journals Valorization of Olive By-Products as Substrates for the Cultivation of Ganoderma lucidum and Pleurotus ostreatus Mushrooms with Enhanced Functional and Prebiotic Properties

Catalysts ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 537 ◽  
Author(s):  
Georgios Koutrotsios ◽  
Marianna Patsou ◽  
Evdokia K. Mitsou ◽  
Georgios Bekiaris ◽  
Maria Kotsou ◽  
...  
Keyword(s):  
Pleurotus Ostreatus ◽  
Ganoderma Lucidum ◽  
Positive Impact ◽  
Intestinal Bacteria ◽  
Fold Increase ◽  
Organic Content ◽  
Bifidobacterium Bifidum ◽  
Specific Pattern ◽  
Total Phenolic ◽  
By Products

The successful management of olive by-products constitutes a major challenge due to their huge volume, high organic content, and toxicity. Olive-mill wastes (TPOMW) and olive pruning residues (OLPR) were evaluated as substrates for the cultivation of Ganoderma lucidum and Pleurotus ostreatus. Chemical composition, glucans, total phenolic content, and antioxidant activity were measured in mushrooms, and their prebiotic potential was assessed by examining their effect on the growth of four intestinal bacteria. Several substrates based on olive by-products had a positive impact on P. ostreatus mushroom production, whereas only one performed adequately for G. lucidum. Increased ratios of OLPR to wheat-straw resulted in an increase of crude protein content in P. ostreatus fruit-bodies by up to 42%, while G. lucidum mushrooms from OLPR-based substrates exhibited an up to three-fold increase in α-glucan, or a significant enhancement of β-glucan content, when compared to beech sawdust (control). The mushrooms’ FTIR spectra confirmed the qualitative/quantitative differentiation detected by standard assays. In regard to prebiotic properties, mushrooms powder supported or even enhanced growth of both Lactobacillus acidophilus and L. gasseri after 24/48 h of incubation. In contrast, a strain-specific pattern was observed in bifidobacteria; mushrooms hindered Bifidobacterium bifidum growth, whereas they supported a similar-to-glucose growth for B. longum.

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