scholarly journals Qualitative and Quantitative Analysis of Dexamethasone in Rheumatic Pain Herbal Medicine Using Thin-Layer Chromatography (TLC) – Densitometry

2021 ◽  
Vol 2 (1) ◽  
pp. 10-22
Author(s):  
Desy Ayu Irma Permatasari ◽  
Novita Kurniasri ◽  
Muladi Putra Mahardika
Keyword(s):  
Herbal Medicine ◽  
Functional Groups ◽  
Qualitative Method ◽  
Chemical Contamination ◽  
Rheumatic Pain ◽  
Organoleptic Test ◽  
Result Show ◽  
Color Purple ◽  
Layer Chromatography

Chemical medicine is chemical substances that is used as the main ingredient of chemical drugs. This compound is usually added to herbal medicine preparation to strengthen the indication of the traditional medicine.Chemical medicine was prohibited from being added to traditional medicinal preparations. But in reality, a lot of medicinal herbs could have contained medicinal chemicals on the market sale. This purpose of this research was to know the chemical contamination of the dexamethasone also the concentration contained in the rheumatic pain herbal medicine. This research was used three kind of rheumatic pain herbal medicine sample to identify dexamethasone, the sample code is S1, S2, and S3. The analysis of qualitative method are organoleptic test, FTIR characteristic test, TLC evaluation. The analysis of quantitative were purposed to know the dexamethasone concentration contained on the rheumatic pain herbal medicine using TLC-Densitometric method. The result show that the three sample of rheumathic pain herbal medicine were contaminated by dexamethasone chemical medicine. Based on organoleptic test, the results show color, smell, and taste. Characterization of the dexamethasone using FTIR were to know functional groups of dexamethasone contained in the rheumatic pain herbal medicine sample, the functional groups of the sample S1, S2, and S3 were compared to dexamethasone standard. To identify of TLC method, obtained Rf value of dexamethasone standard and the sample, visualizing a stain color purple-fluorescence on the UV 254 nm. The analysis of quantitative dexamethasone concentration using TLC-Densitometric showed the presence of dexamethasone in the sample for S1, S2 and S3. The concetration of dexamethasone obtained of sample S1, S2, S3 were 1014.64 µg/g ; 131.15 µg/g ; 135.54 µg/g respectively.

scholarly journals Analisis Natrium Diklofenak Dalam Sampel Jamu Pegal Linu Yang Dijual Di Kabupaten Semarang Secara Klt-Spektrofotometri Uv-Vis

2021 ◽  
Vol 3 (2) ◽  
pp. 163-175
Author(s):  
Moudi Ayuty Viony Padanun ◽  
Tri Minarsih
Keyword(s):  
Quantitative Analysis ◽  
Herbal Medicine ◽  
Qualitative Analysis ◽  
Silica Gel ◽  
Diclofenac Sodium ◽  
Traditional Medicines ◽  
Rheumatic Pain ◽  
Organoleptic Test ◽  
Rf Values ◽  
The Republic

Herbal medicine for rheumatic pain is one of the traditional medicinal products that are massively demanded by the public because it has many benefits. Medicinal Chemicals (MC) are often added to herbal medicine for rheumatic pain to strengthen their properties, one of which is Diclofenac Sodium. Based on the Decree of the Minister of Health of the Republic of Indonesia No. 246 of 2010, traditional medicines are prohibited from containing Medicinal Chemicals (MC). This study aims to analyze the content of Diclofenac Sodium Medicinal Chemicals (MC) in the herbal medicine for rheumatic pain which is sold in Semarang Regency. This type of research was conducted using a laboratory experimental method which descriptively describes the results of the study based on the data obtained. The research method consisted of organoleptic test, qualitative analysis and quantitative analysis of the samples of herbal medicine for aches and pains. Organoleptic test was carried out by tasting the taste, smelling the smell, seeing the color and feeling the dosage form of the Jamu Pegal Linu sample. Qualitative analysis was performed by Thin Layer Chromatography (TLC) and quantitative analysis was performed by UV-Vis Spectrophotometry with 3 samples considered positive. Samples B, D, and E which is sold in Semarang Regency were positive for Diclofenac Sodium based on the Rf values ​​obtained from the samples, namely 0.28, 0.3, and 0.3, which were almost the same as the standard Rf for Sodium Diclofenac, which was 0.26. The stationary phase used a Silica Gel 254 and the mobile phase used Ethyl Acetate and N-Hexane in a ratio of 25: 25. In quantitative analysis, a wavelength of 275 nm was obtained with a linear equation y = 0.0245x + 0.0989 and a value of r = 0.9994 with a concentration of obtained in samples B, D, E were 39.27%, 2.67% and 4.9%, respectively. ABSTRAK Jamu Pegal Linu merupakan salah satu produk obat tradisional yang banyak diminati oleh masyarakat karena memiliki banyak manfaat. Bahan Kimia Obat (BKO) sering ditambahkan pada Jamu Pegal Linu untuk menambah khasiatnya, salah satunya adalah Natrium Diklofenak. Berdasarkan Permenkes RI No. 246 tahun 2010, obat tradisional dilarang mengandung BKO. Penelitian ini bertujuan untuk menganalisis kandungan Natrium Diklofenak pada sediaan Jamu Pegal Linu yang dijual di Kabupaten Semarang. Jenis penelitian dilakukan menggunakan metode eksperimental laboratorium. Metode penelitian terdiri dari uji organoleptis, analisis kualitatif dan kuantitatif. Uji organoleptis dilakukan dengan dengan cara mengamati warna, bau, rasa dan bentuk sampel. Analisis kualitatif dilakukan dengan Kromatografi Lapis Tipis (KLT) dan analisis kuantitatif dilakukan dengan Spektrofotometri UV-Vis. Sampel B, D, dan E yang dijual di Kabupaten Semarang positif mengandung Natrium Diklofenak berdasarkan nilai Rf yang didapatkan dari sampel berturut-turut yaitu 0.28, 0.3, dan 0.3 mendekati nilai Rf baku Natrium Diklfenak yaitu 0.26. Fase diam menggunakan Silica Gel 254 dan fase gerak menggunakan Etil Asetat dan N-Heksan dengan perbandingan 25 : 25. Pada analisis kuantitatif diperoleh panjang gelombang 275 nm dengan persamaan garis linier y = 0,0245x + 0,0989 dan nilai r = 0.9994 dengan kadar pada sampel B, D, E berturut-turut adalah 39.27%, 2.67% dan 4.9%.

KARAKTERISTIK ABON IKAN GABUS (Channa striata) DENGAN SUBTITUSI KLUWIH (Artocarpus camansi)

2018 ◽  
Vol 16 (2) ◽  
pp. 219-226
Author(s):  
Sri Rizqi Annisa ◽  
Dewi Larasati ◽  
Endang Bekti K
Keyword(s):  
Protein Content ◽  
Water Content ◽  
Analysis Of Variance ◽  
Fiber Content ◽  
Substitution Treatment ◽  
Fish Fillet ◽  
Organoleptic Test ◽  
Average Water ◽  
Channa Striata

The aim of this study was to determine the characterization of shredded mureel fish with kluwih substitution on water content, protein content, fiber content and organoleptic (preference for crispness and taste). This study uses a simple Randomized Complete Design (RCD) with the substitution treatment of kluwih and mureel fish, with the following ratio: S1 (240g: 60g), S2 (210g: 90g), S3 (180g: 120g), S4 (150g: 150g), S5 (120g: 180g). Data were analyzed statistically by analysis of variance and if there was a significant effect, further testing was done with BNJ at the level of 5%. The results showed that kluwih substitution in the manufacture of mureel fish shredded had an average: water content of 8.33-10.62%, protein :16.83-22.00%, fiber : 6.79-6.99%, score crispness 2-6.12, taste score 2.6-6.6. Based on the results of the analysis of the variety of kluwih substitutes and mureel fish have a significant effect on water content, protein content and crisp organoleptic test, taste on mureel fish fillet, and no significant effect on fiber content. The best kluwih substitution in S3 treatment with 120 grams of substitute kluwih and 180 grams of mureel fish.

Application of FT Spectroscopy in the Study of Fungi

2008 ◽  
Vol 59 (2) ◽  
pp. 212-215 ◽  
Author(s):  
Catalin Tanase ◽  
Aurel Pui
Keyword(s):  
Functional Groups ◽  
Toxic Substances ◽  
Ft Spectroscopy

The present study is devoted to the IR�FT characterization of some fungi species, to the identification of their main functional groups, as well as to emphasize the presence of some toxic substances in the structure of certain sporiferous plants.

scholarly journals Chemical design and characterization of cellulosic derivatives containing high-nitrogen functional groups: Towards the next generation of energetic biopolymers

2021 ◽  
Author(s):  
Ahmed Fouzi Tarchoun ◽  
Djalal Trache ◽  
Thomas M. Klapötke ◽  
Amir Abdelaziz ◽  
Mehdi Derradji ◽  
...  
Keyword(s):  
Functional Groups ◽  
Next Generation ◽  
High Nitrogen ◽  
Chemical Design

scholarly journals Gene Cloning and Molecular Characterization of a Two-Enzyme System Catalyzing the Oxidative Detoxification of β-Endosulfan

2002 ◽  
Vol 68 (12) ◽  
pp. 6237-6245 ◽  
Author(s):  
Tara D. Sutherland ◽  
Irene Horne ◽  
Robyn J. Russell ◽  
John G. Oakeshott
Keyword(s):  
Shuttle Vector ◽  
Open Reading Frame ◽  
Cosmid Library ◽  
Flavin Mononucleotide ◽  
Flavin Reductase ◽  
Reading Frame ◽  
Reductase Gene ◽  
Degradation Activity ◽  
Layer Chromatography

ABSTRACT The gram-positive bacterium Mycobacterium sp. strain ESD is able to use the cyclodiene insecticide endosulfan as a source of sulfur for growth. This activity is dependent on the absence of sulfite or sulfate in the growth medium. A cosmid library of strain ESD DNA was constructed in a Mycobacterium-Escherichia coli shuttle vector and screened for endosulfan-degrading activity in Mycobacterium smegmatis, a species that does not degrade endosulfan. Using this method, we identified a single cosmid that conferred sulfur-dependent endosulfan-degrading activity on the host strain. An open reading frame (esd) was identified within this cosmid that, when expressed behind a constitutive promoter in a mycobacterial expression vector, conferred sulfite- and sulfate-independent β-endosulfan degradation activity on the recombinant strain. The translation product of this gene (Esd) had up to 50% sequence identity with an unusual family of monooxygenase enzymes that use reduced flavins, provided by a separate flavin reductase enzyme, as cosubstrates. An additional partial open reading frame was located upstream of the Esd gene that had sequence homology to the same monooxygenase family. A flavin reductase gene, identified in the M. smegmatis genome, was cloned, expressed, and used to provide reduced flavin mononucleotide for Esd in enzyme assays. Thin-layer chromatography and gas chromatography analyses of the enzyme assay mixtures revealed the disappearance of β-endosulfan and the appearance of the endosulfan metabolites, endosulfan monoaldehyde and endosulfan hydroxyether. This suggests that Esd catalyzes the oxygenation of β-endosulfan to endosulfan monoaldehyde and endosulfan hydroxyether. Esd did not degrade either α-endosulfan or the metabolite of endosulfan, endosulfan sulfate.

FTIR and Raman spectroscopy characterization of functional groups in various rank coals

Fuel ◽  
2017 ◽  
Vol 206 ◽  
pp. 555-563 ◽  
Author(s):  
Xueqiu He ◽  
Xianfeng Liu ◽  
Baisheng Nie ◽  
Dazhao Song
Keyword(s):  
Raman Spectroscopy ◽  
Functional Groups ◽  
Ftir And Raman Spectroscopy

scholarly journals Characterization of functional groups in estuarine dissolved organic matter by DNP‐enhanced 15N and 13C solid‐state NMR

ChemPhysChem ◽  
2021 ◽  
Author(s):  
Florian Venel ◽  
Hiroki Nagashima ◽  
Andrew G.M. Rankin ◽  
Christelle Anquetil ◽  
Vytautas Klimavicius ◽  
...  
Keyword(s):  
Organic Matter ◽  
Solid State ◽  
Dissolved Organic Matter ◽  
Functional Groups ◽  
Solid State Nmr

Synthesis and chromatographic characterization of [Tc-99m]technetium-humic acid species

2000 ◽  
Vol 88 (2) ◽  
Author(s):  
D. Rößler ◽  
K. Franke ◽  
R. Süß ◽  
E. Becker ◽  
H. Kupsch
Keyword(s):  
Humic Acid ◽  
Stannous Chloride ◽  
Gel Permeation Chromatography ◽  
Paper Electrophoresis ◽  
Gel Permeation ◽  
Soil Humic Acid ◽  
Chromatographic Characterization ◽  
Ph Dependent ◽  
Layer Chromatography

A natural moor soil humic acid (HA) was labeled with Tc-99m via reduction of pertechnetate with stannous chloride. The humic acid species obtained were characterized by thin layer chromatography (TLC), gel permeation chromatography (GPC), sequential chromatographic analysis (SCA), paper electrophoresis and micropore filtration. Labeling was found to take place in all ranges of molecular weight. Due to the complex humic acid composition and the formation of hydroxo species the labeling yields strongly depend on the separation conditions, ranging from 42% to 80%. The pH-dependent distribution of mobile and immobile species was determined by SCA for HTcO

Sign in / Sign up

Export Citation Format

Share Document