scholarly journals Analisis Natrium Diklofenak Dalam Sampel Jamu Pegal Linu Yang Dijual Di Kabupaten Semarang Secara Klt-Spektrofotometri Uv-Vis

2021 ◽  
Vol 3 (2) ◽  
pp. 163-175
Author(s):  
Moudi Ayuty Viony Padanun ◽  
Tri Minarsih
Keyword(s):  
Quantitative Analysis ◽  
Herbal Medicine ◽  
Qualitative Analysis ◽  
Silica Gel ◽  
Diclofenac Sodium ◽  
Traditional Medicines ◽  
Rheumatic Pain ◽  
Organoleptic Test ◽  
Rf Values ◽  
The Republic

Herbal medicine for rheumatic pain is one of the traditional medicinal products that are massively demanded by the public because it has many benefits. Medicinal Chemicals (MC) are often added to herbal medicine for rheumatic pain to strengthen their properties, one of which is Diclofenac Sodium. Based on the Decree of the Minister of Health of the Republic of Indonesia No. 246 of 2010, traditional medicines are prohibited from containing Medicinal Chemicals (MC). This study aims to analyze the content of Diclofenac Sodium Medicinal Chemicals (MC) in the herbal medicine for rheumatic pain which is sold in Semarang Regency. This type of research was conducted using a laboratory experimental method which descriptively describes the results of the study based on the data obtained. The research method consisted of organoleptic test, qualitative analysis and quantitative analysis of the samples of herbal medicine for aches and pains. Organoleptic test was carried out by tasting the taste, smelling the smell, seeing the color and feeling the dosage form of the Jamu Pegal Linu sample. Qualitative analysis was performed by Thin Layer Chromatography (TLC) and quantitative analysis was performed by UV-Vis Spectrophotometry with 3 samples considered positive. Samples B, D, and E which is sold in Semarang Regency were positive for Diclofenac Sodium based on the Rf values ​​obtained from the samples, namely 0.28, 0.3, and 0.3, which were almost the same as the standard Rf for Sodium Diclofenac, which was 0.26. The stationary phase used a Silica Gel 254 and the mobile phase used Ethyl Acetate and N-Hexane in a ratio of 25: 25. In quantitative analysis, a wavelength of 275 nm was obtained with a linear equation y = 0.0245x + 0.0989 and a value of r = 0.9994 with a concentration of obtained in samples B, D, E were 39.27%, 2.67% and 4.9%, respectively. ABSTRAK Jamu Pegal Linu merupakan salah satu produk obat tradisional yang banyak diminati oleh masyarakat karena memiliki banyak manfaat. Bahan Kimia Obat (BKO) sering ditambahkan pada Jamu Pegal Linu untuk menambah khasiatnya, salah satunya adalah Natrium Diklofenak. Berdasarkan Permenkes RI No. 246 tahun 2010, obat tradisional dilarang mengandung BKO. Penelitian ini bertujuan untuk menganalisis kandungan Natrium Diklofenak pada sediaan Jamu Pegal Linu yang dijual di Kabupaten Semarang. Jenis penelitian dilakukan menggunakan metode eksperimental laboratorium. Metode penelitian terdiri dari uji organoleptis, analisis kualitatif dan kuantitatif. Uji organoleptis dilakukan dengan dengan cara mengamati warna, bau, rasa dan bentuk sampel. Analisis kualitatif dilakukan dengan Kromatografi Lapis Tipis (KLT) dan analisis kuantitatif dilakukan dengan Spektrofotometri UV-Vis. Sampel B, D, dan E yang dijual di Kabupaten Semarang positif mengandung Natrium Diklofenak berdasarkan nilai Rf yang didapatkan dari sampel berturut-turut yaitu 0.28, 0.3, dan 0.3 mendekati nilai Rf baku Natrium Diklfenak yaitu 0.26. Fase diam menggunakan Silica Gel 254 dan fase gerak menggunakan Etil Asetat dan N-Heksan dengan perbandingan 25 : 25. Pada analisis kuantitatif diperoleh panjang gelombang 275 nm dengan persamaan garis linier y = 0,0245x + 0,0989 dan nilai r = 0.9994 dengan kadar pada sampel B, D, E berturut-turut adalah 39.27%, 2.67% dan 4.9%.

Phytochemical evaluation of leaf extracts of Naringi crenulata (roxb.) Nicolson

2016 ◽  
Vol 5 (11) ◽  
pp. 5110
Author(s):  
Sartaj Ahmad Allayie ◽  
Mushtaq Ahmed Parray* ◽  
Bilal Ahmad Bhat ◽  
S. Hemalatha
Keyword(s):  
Quantitative Analysis ◽  
Solvent System ◽  
Great Promise ◽  
Phytochemical Analysis ◽  
Bioactive Constituents ◽  
Leaf Extracts ◽  
Traditional Medicines ◽  
The People ◽  
Wide Range ◽  
Rf Values

The use of traditional medicines holds a great promise as an easily available source as effective medicinal agents to cure a wide range of ailments among the people particularly in tropical developing countries like India. The present study investigates the qualitative and quantitative analysis of the major bioactive constituents of N. crenulata leaf extracts. The extractive values of aqueous, acetone and chloroform extracts were found to be 11.34, 4.24 and 6.06 respectively. Qualitative phytochemical analysis of these three solvent extracts confirm the presence of Alkaloids, Saponins, Flavonoids and Phenolic compounds in all the three extracts; however, these phytochemicals were more significant in aqueous extract. Quantitative analysis was carried out using TLC method by different solvent system. Amongst various solvent systems, Butanol: acetic acid: water (9: 0.9: 0.1 v/v/v) shows maximum resolution and number of spots produced at long UV (365 nm) and under iodine vapours. The TLC chromatograms constituted different coloured phytochemical compounds with different Rf values. It can be conveniently used to evaluate the quality of different area samples. This indicates that the leaves can be useful for treating different diseases because the therapeutic activity of a plant is due to the presence of particular class of compounds and thus can serve as potential sources of useful drugs in future.

scholarly journals The total plate count of beras kencur herbal medicine in sub-district market Tulungagung city 2018

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Eka Puspitasari ◽  
Chalies Diah Pratiwi ◽  
Tri Wahyuni
Keyword(s):  
Herbal Medicine ◽  
Disease Transmission ◽  
Microbial Contamination ◽  
Raw Materials ◽  
Sampling Technique ◽  
Plate Count ◽  
Traditional Herbal Medicine ◽  
Traditional Medicines ◽  
Hygiene Factors ◽  
The Republic

Herbal medicine is a traditional drink made from natural ingredients for human health and is made with methods and equipment that are easier in its simplicity which often does not pay attention to hygiene factors and can be a medium for disease transmission. Based on the Regulation of the Head of the Republic of Indonesia Drug and Food Control Agency No. 12 of 2014 concerning requirements for drug fluids in the form of microbial contamination / ALT ≤104 colonies/ml. The purpose of this study was to study the estimated numbers of traditional herbs sold in the Tulungagung District market. The research design used is descriptive. The sampling technique uses a total sampling technique of 6 samples from 4 different markets. Sample meeting with the ALT method in May 2018 in the microbiology laboratory of STIKes Hutama Abdi Husada Tulungagung. The results of the study found that three out of six samples of traditional herbal medicine of kencur rice exceeded the microbial contamination threshold with an average yield of 1 x 107 CFU / ml. The study concludes is that 50% of the sample is not suitable for consumption and 50% of the sample is suitable for consumption. This requires Regulation of BPOM RI Number 12 the year 2014 Regarding Quality Requirements for Traditional Medicines, namely ≤104 colonies/ml. Herbal traders need to pay attention to raw materials, processing, equipment, air used, and containers used.

scholarly journals AN ANALYSIS OF THE EFFECTIVENESS ATTENDANCE ASSESSMENTS ON THE PERFORMANCE OF EMPLOYEES AT KPRI ‘BETIK’ GAWI KOTA BANDAR LAMPUNG

Jurnal AKTUAL ◽  
2020 ◽  
Vol 17 (2) ◽  
pp. 82
Author(s):  
Yugestina Yugestina
Keyword(s):  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Employee Performance ◽  
Positive Influence ◽  
Sampling Techniques ◽  
Employee Productivity ◽  
Probability Sampling ◽  
The Republic ◽  
Relationship Of ◽  
The Relationship

This study aims to determine the effect of attendance assessments on the performance of employees and administrators at KPRI Betik Gawi Kota Bandar Lampung. This study uses a qualitative analysis approach (used to find out how the relationship of the influence of motivation on employee productivity of the Republic of Indonesia Employee Cooperative) and quantitative analysis (used to determine how much influence the absenteeism assessment on employee performance). Samples in this study used non-probability sampling techniques. Based on the results of the analysis it was concluded that there was a positive influence between the analysis of the effectiveness of attendance assessments on employee performance at KPRI Betik Gawi Kota  Bandar Lampung.

scholarly journals Qualitative and Quantitative Analysis of Dexamethasone in Rheumatic Pain Herbal Medicine Using Thin-Layer Chromatography (TLC) – Densitometry

2021 ◽  
Vol 2 (1) ◽  
pp. 10-22
Author(s):  
Desy Ayu Irma Permatasari ◽  
Novita Kurniasri ◽  
Muladi Putra Mahardika
Keyword(s):  
Herbal Medicine ◽  
Functional Groups ◽  
Qualitative Method ◽  
Chemical Contamination ◽  
Rheumatic Pain ◽  
Organoleptic Test ◽  
Result Show ◽  
Color Purple ◽  
Layer Chromatography

Chemical medicine is chemical substances that is used as the main ingredient of chemical drugs. This compound is usually added to herbal medicine preparation to strengthen the indication of the traditional medicine.Chemical medicine was prohibited from being added to traditional medicinal preparations. But in reality, a lot of medicinal herbs could have contained medicinal chemicals on the market sale. This purpose of this research was to know the chemical contamination of the dexamethasone also the concentration contained in the rheumatic pain herbal medicine. This research was used three kind of rheumatic pain herbal medicine sample to identify dexamethasone, the sample code is S1, S2, and S3. The analysis of qualitative method are organoleptic test, FTIR characteristic test, TLC evaluation. The analysis of quantitative were purposed to know the dexamethasone concentration contained on the rheumatic pain herbal medicine using TLC-Densitometric method. The result show that the three sample of rheumathic pain herbal medicine were contaminated by dexamethasone chemical medicine. Based on organoleptic test, the results show color, smell, and taste. Characterization of the dexamethasone using FTIR were to know functional groups of dexamethasone contained in the rheumatic pain herbal medicine sample, the functional groups of the sample S1, S2, and S3 were compared to dexamethasone standard. To identify of TLC method, obtained Rf value of dexamethasone standard and the sample, visualizing a stain color purple-fluorescence on the UV 254 nm. The analysis of quantitative dexamethasone concentration using TLC-Densitometric showed the presence of dexamethasone in the sample for S1, S2 and S3. The concetration of dexamethasone obtained of sample S1, S2, S3 were 1014.64 µg/g ; 131.15 µg/g ; 135.54 µg/g respectively.

scholarly journals Identifikasi Kandungan Merkuri pada Beberapa Krim Pemutih yang Beredar di Pasaran (Studi dilakukan di Pasar DTC Wonokromo Surabaya)

2017 ◽  
Vol 2 (2) ◽  
pp. 35-40
Author(s):  
Fatma Ariska Trisnawati ◽  
Cicik Herlina Yulianti ◽  
Tamara Gusti Ebtavanny
Keyword(s):  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Atomic Absorption ◽  
Active Ingredient ◽  
Atomic Absorption Spectrophotometry ◽  
Analysis Method ◽  
Absorption Spectrophotometry ◽  
Registration Number ◽  
The Republic ◽  
The One

ABSTRAKRadiasi sinar ultraviolet matahari dapat menyebabkan berbagai permasalahan pada kulit. Untuk mengatasinya perlu adanya perawatan menggunakan kosmetik, salah satunya yaitu krim pemutih wajah (Whitening Cream). Merkuri merupakan salah satu bahan aktif yang sering direkomendasikan karena ion merkuri dianggap dapat menghambat sintesis melamin pigmen kulit di sel melanosit. Menurut Peraturan Badan Pengawas Obat dan Makanan Republik Indonesia nomor HK.03.01.23.07.11.6662 tahun 2011 persyaratan logam berat jenis merkuri (Hg) adalah tidak lebih dari 1 mg/kg atau 1 mg/L (1ppm).Tujuan dari penelitian ini adalah untuk mengetahui perbedaan kadar merkuri pada produk kosmetik krim pemutih yang tidak memiliki nomor registrasi BPOM dan yang memiliki nomor registrasi BPOM yang beredar di pasaran. Serta untuk mengetahui bahwa sediaan kosmetik krim pemutih wajah yang beredar di pasaran telah memenuhi syarat yang ditetapkan BPOM. Sampel dalam penelitian ini adalah sebanyak 18 sampel dengan kriteria 9 krim pemutih yang tidak memiliki nomor registrasi BPOM dan 9 krim pemutih yang memiliki nomor registrasi BPOM. Metode yang digunakan dalam penelitian ini adalah analisa kualitatif menggunakan metode pereaksi warna dengan Kalium Iodida dan analisa kuantitatif dengan metode spektrofotometri serapan atom. Hasil penelitian menunjukkan 2 dari 18 sampel mengandung merkuri. Berdasarkan uji kuantitatif menunjukkan kadar merkuri pada produk krim pemutih yangmemiliki nomor registrasi BPOM dengan varian A1 sebesar 224,04 ± 0,35 mg/kg, dan untuk varian A2 adalah 188,20 ± 0,28 mg/kg. Sehingga tidak semua kosmetik krim pemutih wajah yang beredar dipasaran memenuhi syarat yang ditetapkan oleh BPOM.Kata Kunci: BPOM, Krim Pemutih, MerkuriABSTRACTRadiation of sun's ultraviolet can cause skin problems. To overcome this problem should has cosmetic treatments using one of these is whitening cream. Mercury is the one of active ingredient who has often add inthe whitening cream because mercury’s ion considered to inhibit the synthesis melanin pigment of the skin in the melanocyte cells. According to the Regulation Agency of Drug and Food of the Republic Indonesia with No. HK. 03.01.23.07.11.6662. 2011, requirements metal usage types of mercury (Hg) is not more than 1 mg/kg or 1 mg/L (1 ppm). The purpose of this study was to determine differences mercury levels in whitening creams cosmetic which products didn’t have a registration number and products which have a registration number BPOM among in the market. And to know that all whitening cream cosmetic among the market has fullfield requirements established by BPOM. Sample use in this study was 18 samples which 9 sample didn’t have a registration number from BPOM and 9 among them have a registration number from BPOM. Analysis method used in this study is qualitative analysis using reaction color with Potassium Iodide and quantitative analysis using atomic absorption spectrophotometry. The results showed that 2 of the 18 samples contained mercury more than what it should. Based of the quantitative analysis shows that there are has differences mercury levelsfrom whitening cream product which has the registration number of BPOM with sample A1 variant is 224.04 ± 0.35 mg / kg, and for the A2 variant is 188.20 ± 0.28 mg / kg. Not all whitening cream cosmetic among the market has fullfield requirements established by BPOM.Keywords: BPOM, mercury, whitening cream.

scholarly journals Determination of Flavonoids Compounds of Three Species and Different Harvesting Periods in Crataegi folium Based on LC-MS/MS

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1602
Author(s):  
Ya-Ping Guo ◽  
Hong Yang ◽  
Ya-Li Wang ◽  
Xiao-Xiang Chen ◽  
Ke Zhang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Quantitative Method ◽  
Total Content ◽  
Qualitative And Quantitative Analysis ◽  
Pharmacological Activities ◽  
Time Saving ◽  
Qualitative And Quantitative ◽  
First Time

Crataegi folium have been used as medicinal and food materials worldwide due to its pharmacological activities. Although the leaves of Crataegus songorica (CS), Crataegus altaica (CA) and Crataegus kansuensis (CK) have rich resources in Xinjiang, China, they can not provide insights into edible and medicinal aspects. Few reports are available on the qualitative and quantitative analysis of flavonoids compounds of their leaves. Therefore, it is necessary to develop efficient methods to determine qualitative and quantitative flavonoids compounds in leaves of CS, CA and CK. In the study, 28 unique compounds were identified in CS versus CK by qualitative analysis. The validated quantitative method was employed to determine the content of eight flavonoids of the leaves of CS, CA and CK within 6 min. The total content of eight flavonoids was 7.8–15.1 mg/g, 0.1–9.1 mg/g and 4.8–10.7 mg/g in the leaves of CS, CA and CK respectively. Besides, the best harvesting periods of the three species were from 17th to 26th September for CS, from 30th September to 15th October for CA and CK. The validated and time-saving method was successfully implemented for the analysis of the content of eight flavonoids compounds in CS, CA and CK for the first time.

scholarly journals The toxic effects of chloroquine and hydroxychloroquine on skeletal muscle: a systematic review and meta-analysis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Claudia Cristina Biguetti ◽  
Joel Ferreira Santiago Junior ◽  
Matthew William Fiedler ◽  
Mauro Toledo Marrelli ◽  
Marco Brotto
Keyword(s):  
Systematic Review ◽  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Observational Studies ◽  
Skeletal Muscles ◽  
Case Reports ◽  
Meta Analysis ◽  
Toxic Effects ◽  
Drug Induced ◽  
Qualitative And Quantitative

AbstractThe aim of this systematic review was to perform qualitative and quantitative analysis on the toxic effects of chloroquine (CQ) and hydroxychloroquine (HCQ) on skeletal muscles. We designed the study according to PRISMA guidelines. Studies for qualitative and quantitative analyses were selected according to the following inclusion criteria: English language; size of sample (> 5 patients), adult (> age of 18) patients, treated with CQ/HCQ for inflammatory diseases, and presenting and not presenting with toxic effects on skeletal muscles. We collected data published from 1990 to April 2020 using PubMed, Cochrane Library, EMBASE, and SciELO. Risk of bias for observational studies was assessed regarding the ROBIN-I scale. Studies with less than five patients (case reports) were selected for an additional qualitative analysis. We used the software Comprehensive Meta-Analysis at the confidence level of 0.05. We identified 23 studies for qualitative analysis (17 case-reports), and five studies were eligible for quantitative analysis. From case reports, 21 patients presented muscle weakness and confirmatory biopsy for CQ/HCQ induced myopathy. From observational studies, 37 patients out of 1,367 patients from five studies presented muscle weakness related to the use of CQ/HCQ, and 252 patients presented elevated levels of muscle enzymes (aldolase, creatine phosphokinase, and lactate dehydrogenase). Four studies presented data on 34 patients with confirmatory biopsy for drug-induced myopathy. No study presented randomized samples. The chronic use of CQ/HCQ may be a risk for drug-induced myopathy. There is substantiated need for proper randomized trials and controlled prospective studies needed to assess the clinical and subclinical stages of CQ/HCQ -induced muscle myopathy.

DEVELOPMENT OF HPTLC METHOD FOR THE ESTIMATION OF ONDANSETRON AND RANITIDINE IN COMBINED DOSAGE FORM

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (12) ◽  
pp. 42-48
Author(s):  
P. J. Patel ◽  
◽  
D. A Shah ◽  
F. A. Mehta ◽  
U. K. Chhalotiya
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
High Performance ◽  
Dosage Form ◽  
Solvent System ◽  
Rf Values ◽  
Layer Chromatography ◽  
Hptlc Method

A simple, sensitive and precise high performance thin layer chromatographic (HPTLC)method has been developed for the estimation of ondansetron (OND) and ranitidine (RAN) in combination. The method was employed on thin layer chromatography (TLC) and aluminium plates were precoated with silica gel 60 F254 as the stationary phase, while the solvent system was methanol. The Rf values were observed to be 0.5 ± 0.02, and 0.3 ± 0.02 for OND and RAN, respectively. The separated spots were densitometrically analyzed in absorbance mode at 299 nm. This method was linear in the range of 25-300 ng/band for OND and 50-600 ng/band for RAN. The limits of detection for OND and RAN were found to be 3.47 and 1.83 ng/band, respectively. The limits of quantification for OND and RAN were found to be 10.53 and 5.55 ng/band, respectively. The proposed method was validated with respect to linearity, accuracy, precision and robustness. The method was successfully applied to the estimation of OND and RAN in combined dosage form.

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