scholarly journals To evaluate the anticonvulsant activity of ethanolic extract of Moringa oleifera (drumstick leaves) in albino mice

2017 ◽  
Vol 6 (10) ◽  
pp. 2491
Author(s):  
Sushma V. Naidu ◽  
Harsha R. ◽  
Jyothsnya S.
Keyword(s):  
Anticonvulsant Activity ◽  
Hind Limb ◽  
Moringa Oleifera ◽  
Ethanolic Extract ◽  
P Value ◽  
Maximal Electroshock ◽  
Maximal Electroshock Seizure ◽  
Isolation And Identification ◽  
Standard Drug ◽  
Albino Mice

Background: To evaluate the anti-convulsant activity of ethanolic extract of Moringa oleifera (Drum stick leaves) in seizure induced albino mice and to compare it with standard drug Sodium valproate.Methods: Swiss albino mice of either sex weighing around 25-30g were randomly selected and divided into four groups of six mice each. Group 1: control- treated with gum acacia. Group 2: Standard - Valproic acid 40mg/kg body weight. Group 3: T1- ethanolic extract of Moringa oleifera (150mg/kg). Group 4: T2 - ethanolic extract of Moringa oleifera (300mg/kg). All drugs were administered orally one hour prior to induction of seizure. The anticonvulsant activity was screened using maximal electroshock seizure (MES) model and pentylenetetrazole (PTZ) model.Results: Results were analysed by ANOVA followed by Bonferroni’s post hoc test. Abolition of Tonic hind limb extension was taken as the protective end point against MES induced seizures and prolongation of seizure latency in PTZ model.At both the doses the ethanolic extract of Moringa oleifera significantly (p value <0.05) reduced the duration of hind limb extension in MES test and also significantly (p value <0.05) delayed the onset of clonic seizures in PTZ induced convulsion when compared with control group.Conclusions: On comparing the percentage protection offered by Moringa oleifera leaves against both MES and PTZ model, it possesses significant anticonvulsant activity at both doses, with more efficacy at 300mg/kg BW indicating that the test drug can prove a very promising drug for treatment of epilepsy. Further studies are required for isolation and identification of the active constituent.

scholarly journals Effect of ethanolic extract of Centella asiatica on maximal electroshock seizure in albino mice

2020 ◽  
Vol 8 (10) ◽  
pp. 3678
Author(s):  
Ahmed A. Kannoor ◽  
Ramani P. T.
Keyword(s):  
Hind Limb ◽  
Herbal Medicines ◽  
Memory Loss ◽  
Ethanolic Extract ◽  
Centella Asiatica ◽  
Maximal Electroshock ◽  
Maximal Electroshock Seizure ◽  
Standard Drug ◽  
Soxhlet Apparatus ◽  
Albino Mice

Background: Animal models had always served as integral part in introducing newer drugs for epilepsy. India has long tradition of using herbal medicines. Centella asiatica is an ingredient of various ayurvedic preparations to treat brain related disorders like insanity, memory loss and epilepsy. This research is done to find out the usefulness of Centella asiatica in epilepsy.  Methods: Ethanolic extract of the plant is prepared using Soxhlet apparatus. Experimental seizure is induced in albino mice by maximal electroshock method. Resulting seizure goes through different phases. Duration of the phase of hind limb extension was the measured data. Clinically used anti epileptic drugs can abolish hind limb extension. Effect is compared to that of standard drug phenytoin. Four different doses of the plant extract; 100mg, 200mg, 500mg and 1000mg per kilogram bodyweight was given orally. Statistical analysis of data was done by one way ANOVA and Dunnett test.Results: Ethanolic extract of Centella asiatica exhibited statistically significant protection from maximal electroshock seizures. All given doses of the extract had p<0.05 when compared to control.Conclusions: Centella asiatica is potential source of anti-epileptic drug. Detailed phytochemical studies and animal experiments are recommended.

scholarly journals ANTICONVULSANT ACTIVITY OF RAUWOLFIA TETRAPHYLLA LEAF EXTRACT IN SWISS ALBINO MICE

2019 ◽  
pp. 377-380
Author(s):  
Aaditya Singh ◽  
SHALINI TRIPATHI ◽  
SINGH PN
Keyword(s):  
Gallic Acid ◽  
Anticonvulsant Activity ◽  
High Performance ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Maximal Electroshock ◽  
Maximal Electroshock Seizure ◽  
Swiss Albino Mice ◽  
Albino Mice ◽  
Layer Chromatography

Objective: Rauvolfia tetraphylla is a plant potentially applicable in Ayurvedic and Unani System of Medicine for the treatment of various diseases. However, the anticonvulsant activity of this plant has not been reported and studied. Therefore, the ethanolic extract of leaf from the plant R. tetraphylla is used to evaluate anticonvulsant activity. Methods: Anticonvulsant activity was screened using maximal electroshock seizure (MES) model and pentylenetetrazole (PTZ)-induced seizure model in Swiss albino mice. The ethanolic extract was also evaluated for rutin and gallic acid content by high-performance thin-layer chromatography studies. Results: Rutin and gallic acid contents were found as 15.60% and 7.81%, respectively. Ethanolic leaf extract (100–800 mg/kg) significantly reduced the duration of seizures which was induced by MES. The same doses also protected animals from PTZ-induced tonic seizures. Conclusion: The study demonstrates that R. tetraphylla plant leaves have significant anticonvulsant activity.

scholarly journals EVALUATION OF ANTICONVULSANT ACTIVITY OF MIMOSA PUDICA ROOT LINN IN SWISS ALBINO MICE

2016 ◽  
Vol 8 (9) ◽  
pp. 49 ◽  
Author(s):  
Prathima C. ◽  
Shashikumara . ◽  
Thippeswamy T. ◽  
Jayanthi M. K.
Keyword(s):  
Anticonvulsant Activity ◽  
Hind Limb ◽  
Latency Period ◽  
Ethanolic Extract ◽  
Epileptic Activity ◽  
Extension Phase ◽  
Mimosa Pudica ◽  
Soxhlet Apparatus ◽  
Seizure Models ◽  
Albino Mice

<p><strong>Objective: </strong>To evaluate the anticonvulsant activity of ethanolic extract of <em>Mimosa pudica</em> root (EMPR) in experimental mice models.</p><p><strong>Methods</strong><strong>: </strong>Ethanolic extract of root parts of <em>Mimosa pudica</em> (EMPR) was prepared by a continuous method using soxhlet apparatus. EMPR in doses of 1000, 2000 mg/kg body wt along with valproate were administrated to albino mice by oral route and anti-epileptic activity was assessed by maximal electroshock (MES) and pentylenetetrazole (PTZ) induced seizure models. Abolition of tonic hind limb extension phase and an increase in seizure latency period, when compared to control group, were taken as a measure of protection in MES and PTZ induced convulsion models respectively.</p><p><strong>Results: </strong>EMPR in the dose of 1000 and 2000 mg/kg body wt of mice showed significant anti-epileptic property in both MES and PTZ induced seizure models. There was a significant abolition of tonic hind limb extension phase in MES model. There was also a significant increase in seizure latency period in PTZ induced seizure model.<strong></strong></p><p><strong>Conclusion: </strong>Results suggest that ethanolic extract of <em>Mimosa pudica</em> roots possess significant anti-epileptic activity. Further investigations are required to determine its active constituents and also its antiepileptic mechanism of action.</p>

scholarly journals Anticonvulsant Effects of Aerial Parts of Verbena officinalis Extract in Mice: Involvement of Benzodiazepine and Opioid Receptors

2017 ◽  
Vol 22 (4) ◽  
pp. 632-636 ◽  
Author(s):  
Amir Rashidian ◽  
Fatemeh Kazemi ◽  
Saeed Mehrzadi ◽  
Ahmad Reza Dehpour ◽  
Shahram Ejtemai Mehr ◽  
...  
Keyword(s):  
Anticonvulsant Activity ◽  
Hind Limb ◽  
Ethanolic Extract ◽  
Maximal Electroshock ◽  
Dependent Manner ◽  
Reference Drug ◽  
Mes Test ◽  
Aerial Parts ◽  
Tonic Extension ◽  
Dose Dependent

To evaluate the anticonvulsant activity of the aerial parts of Verbena officinalis used traditionally by local Iranians for the treatment of convulsion. The anticonvulsant activity of the extract was assessed in pentylenetetrazole (PTZ) and maximal electroshock (MES) induced seizures in mice. Diazepam was used as reference drug. In addition, for investigating the mechanism of V officinalis in PTZ model, flumazenil and naloxone were injected before V officinalis. The extract showed no toxicity and significantly increased the period taken before the onset and decreased the duration of the seizures induced by PTZ. In the MES test, V officinalis displayed significant reduction in hind limb tonic extension duration in a dose-dependent manner. The results propose that V officinalis ethanolic extract has anticonvulsant activity against seizure. It seems that these effects may be related to potentiating of GABAergic system. Moreover, this study supports the use of this plant by local Iranians in order to treat convulsion.

scholarly journals STUDY OF THE ANTICONVULSANT ACTIVITY OF ETHANOLIC EXTRACT OF ROOT OF ACORUS CALAMUS IN ALBINO RATS

2019 ◽  
Vol 12 (1) ◽  
pp. 185
Author(s):  
Shipra Kaushik ◽  
Kalpana Gohain
Keyword(s):  
Normal Saline ◽  
Anticonvulsant Activity ◽  
Ethanolic Extract ◽  
Albino Rats ◽  
Acorus Calamus ◽  
Maximal Electroshock ◽  
Maximal Electroshock Seizure ◽  
Onset Of Action ◽  
Electrical Shock ◽  
Seizure Models

Objective: Root of Acorus calamus has been traditionally used as an anticonvulsant. The aim of the study is to assess the anticonvulsant activity of ethanolic extract of A. calamus (EEAC) by maximal electroshock seizure (MES) and pentylenetetrazol (PTZ)-induced seizure models on albino (Wistar strain) rats.Methods: Albino rats were taken and divided into five groups, each consisting of five rats both for MES and PTZ model. One group was used as control (normal saline 10 ml/kg), one as standard (phenytoin in MES model/diazepam in PTZ model), and three groups for the test drug (EEAC in the doses of 100, 200, and 400 mg/kg). In MES model, maximal electrical shock of 150 mA was passed for 0.2 s through earlobe electrodes after 30 min of giving the drugs and normal saline. Different stages of convulsions were noted down along with time spent by the animal in each phase of convulsions. In PTZ model, PTZ was injected 30 min after giving the drugs and normal saline, and onset of action and severity of convulsions were noted. Data were statistically analyzed by one-way analysis of variance followed by multiple Dunnett’s test.Results: EEAC dose dependently reduced the duration of tonic hind limb extension in MES model, and there was increase in latency and occurrence of convulsions in PTZ model.Conclusion: EEAC has anticonvulsant activity.

scholarly journals STUDY OF THE ANTICONVULSANT ACTIVITY OF ETHANOLIC EXTRACT OF ROOT OF ACORUS CALAMUS IN ALBINO RATS

2019 ◽  
Vol 12 (1) ◽  
pp. 185
Author(s):  
Shipra Kaushik ◽  
Kalpana Gohain
Keyword(s):  
Normal Saline ◽  
Anticonvulsant Activity ◽  
Ethanolic Extract ◽  
Albino Rats ◽  
Acorus Calamus ◽  
Maximal Electroshock ◽  
Maximal Electroshock Seizure ◽  
Onset Of Action ◽  
Electrical Shock ◽  
Seizure Models

Objective: Root of Acorus calamus has been traditionally used as an anticonvulsant. The aim of the study is to assess the anticonvulsant activity of ethanolic extract of A. calamus (EEAC) by maximal electroshock seizure (MES) and pentylenetetrazol (PTZ)-induced seizure models on albino (Wistar strain) rats.Methods: Albino rats were taken and divided into five groups, each consisting of five rats both for MES and PTZ model. One group was used as control (normal saline 10 ml/kg), one as standard (phenytoin in MES model/diazepam in PTZ model), and three groups for the test drug (EEAC in the doses of 100, 200, and 400 mg/kg). In MES model, maximal electrical shock of 150 mA was passed for 0.2 s through earlobe electrodes after 30 min of giving the drugs and normal saline. Different stages of convulsions were noted down along with time spent by the animal in each phase of convulsions. In PTZ model, PTZ was injected 30 min after giving the drugs and normal saline, and onset of action and severity of convulsions were noted. Data were statistically analyzed by one-way analysis of variance followed by multiple Dunnett’s test.Results: EEAC dose dependently reduced the duration of tonic hind limb extension in MES model, and there was increase in latency and occurrence of convulsions in PTZ model.Conclusion: EEAC has anticonvulsant activity.

scholarly journals Drug Interaction analysis-Nigella sativa L.seed (Black Cumin) ethanolicextract on antiseizure activity of Phenobarbitone sodium

2020 ◽  
Vol 11 (2) ◽  
pp. 18-20
Author(s):  
Lovelyn Joseph ◽  
Rejeesh Edavan Puthillath ◽  
Sudarshanram Narayan Rao
Keyword(s):  
Interaction Analysis ◽  
Nigella Sativa ◽  
Ethanolic Extract ◽  
Maximal Electroshock ◽  
Maximal Electroshock Seizure ◽  
Multidisciplinary Therapy ◽  
Swiss Albino Mice ◽  
Isobolographic Analysis ◽  
Albino Mice ◽  
Nigella Sativa Seed

Background: Multidisciplinary therapy is gaining popularity and drug herb interactions is one of the biggest risk factor contributing to therapeutic failures or toxicities. Apart from analysis of one of such Drug-Herb combinations, this work also aims at emphasizing need for ethnopharmacovigilance practices. Aims and Objectives: Herb-Drug Interaction analysis between Nigella sativa seed ethanolic extract and Phenobarbitone sodium in maximal electroshock seizure in Swiss albino mice with a modified isobolographic analysis. Materials and Methods: Experiment model was Maximal electroshock seizure in Swiss albino mice. Dose ratios of the combination tested were NsEE: Phenobarbitone; 3:1(NsEE75%: Pbt25%), 1:1, 1:3. Analysis was done with modified isobolographic analysis. Results: Nigella sativa seed ethanolic extract exhibit synergistic interaction with Phenobarbitone sodium at 1:1 and 1:3 dose ratios and antagonistic interaction at 3:1 combination. The parameter measured was Hind limb tonic extensor phase in maximal electroshock seizure test in Swiss albino mice. Conclusion: The study showed that interaction profile of Nigella sativa extract-phenobarbitone combination is dose dependent and requires well designed posological studies in epileptic patients to formulate dose adjustment guidelines for multidisciplinary therapy with the herb and Phenobarbitone, also to avoid food drug interactions.

scholarly journals EVALUATION OF ANTIEPILEPTIC ACTIVITY OF ETHANOLIC EXTRACT OF AZIMA TETRACANTHA ROOT IN MICE

2016 ◽  
Vol 8 (4) ◽  
pp. 76
Author(s):  
Madhavi Eerike ◽  
Venu Gopala Rao Konda ◽  
Ruckmani Arunachalam ◽  
Umar Dawood
Keyword(s):  
Sodium Valproate ◽  
Anticonvulsant Activity ◽  
Hind Limb ◽  
Ethanolic Extract ◽  
Control Group ◽  
Standard Group ◽  
Maximal Electroshock ◽  
Antiepileptic Activity ◽  
Tonic Extension ◽  
Azima Tetracantha

Objective: To evaluate the antiepileptic activity of ethanolic extract of Azima tetracantha root (EEATR) against Maximal electroshock (MES) and Pentylene tetrazole (PTZ) induced seizures in mice.Methods: 48 adult male mice were used and 4 groups with six in each were allocated to each model. 4 Groups are divided into control, standard and two test groups. Control group received normal saline, standard group, Sodium valproate-200 mg/kg and the two test groups received ethanolic extract of roots of Azima tetracantha (EEATR) 250 and 500 mg/kg respectively. Antiepileptic activity was assessed based on hind limb tonic extension duration, onset of convulsions and mortality. The results were compared with control and standard.Results: In MES model EEATR reduced the duration of hind limb extension (HLE) and seizure protection was 50% and 66.6% with 250 and 500 mg/kg respectively. In PTZ model both the doses of EEATR delayed the onset of clonic phase and prevented death in 50% of animals in group treated with 500 mg/kg EEATR, similar to sodium valproate. Results were analyzed by ANOVA with p<0.05 considered as significant.Conclusion: EEATR has shown anticonvulsant activity in both MES and PTZ models. 500 mg/kg of EEATR has better protection than 250 mg/kg against seizure in MES model and equally efficacious as sodium valproate standard in PTZ model.

Evaluation of the Anticonvulsant Activity of Terpinen-4-ol

2009 ◽  
Vol 64 (1-2) ◽  
pp. 1-5 ◽  
Author(s):  
Damião P. de Sousa ◽  
Franklin F. F. Nóbrega ◽  
Liana C. S. L. de Morais ◽  
Reinaldo N. de Almeida
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Animal Models ◽  
Motor Activity ◽  
Anticonvulsant Activity ◽  
Maximal Electroshock ◽  
Aromatic Plants ◽  
Depressant Effect ◽  
Maximal Electroshock Seizure ◽  
The Central Nervous System

Terpinen-4-ol is a monoterpenoid alcohol and component of the essential oils of several aromatic plants. Similarly to terpinen-4-ol, other monoterpenoid alcohols have shown anticonvulsant activity in convulsion animal models. The present study aimed to investigate the anticonvulsant activity of terpinen-4-ol. Treatment of mice with terpinen-4-ol ( 200 mg/kg) caused a signifi cant decrease in the spontaneous motor activity at 30, 60 and 120 min after administration. Terpinen-4-ol (100 and 200 mg/kg) produced a significant dosedependent increase in the duration of sleeping in mice. Pretreatment of mice with terpinen-4- ol at doses of 100, 200 and 300 mg/kg significantly increased the latency of pentylenetetrazole -induced convulsions. Terpinen-4-ol (200 and 300 mg/kg) also inhibited the induced seizures of picrotoxin. In another model, maximal electroshock seizure, terpinen-4-ol decreased the tonic hind convulsions percentage at the dose of 300 mg/kg. From the overall results we can conclude that terpinen-4-ol showed a depressant effect on the central nervous system and significant anticonvulsant activity.

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