scholarly journals In vitro study on antioxidant activity of methanolic leaf extract of Cassia fistula Linn.

2018 ◽  
Vol 7 (5) ◽  
pp. 849
Author(s):  
Bindhu R. Kamath ◽  
Sabeena Kizhedath
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Methanolic Extract ◽  
Heart Diseases ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
In Vitro Study ◽  
Cassia Fistula ◽  
Methanolic Extracts

Background: The use of medicinal plants for maintaining good health is getting attention worldwide. Antioxidants play an important role to protect damage caused by oxidative stress. In the present study methanolic extracts of Cassia fistula  was determined using DPPH for its antioxidant activity. Phytochemical investigation confirmed the presence of bioactive ingredients in the extract.Methods: The antioxidant activity of methanolic extract of Cassia fistula was evaluated using DPPH free radical assay. DPPH (1,1-diphenyl-2-picrylhydrazyine) free radical analysis is one of the accurate and frequently employed method for evaluating antioxidant activity.Results: The methanolic extracts showed increase in radical scavenging activity as concentration increases. The IC50 values were calculated for the methanolic extract. Ascorbic acid was used as control. Cassia fistula exhibited IC 50 of 79.42µg/ml.Conclusions: Scientific evidence suggests that antioxidants reduce the risk for chronic diseases including cancer and heart disease and infectious diseases. Further evaluation of pharmacological activities of Cassia fistula may prove useful in treatment of cancer and heart diseases.

scholarly journals In vitro study on the antioxidant activity of methanolic leaf extract of Carica papaya

2020 ◽  
Vol 9 (4) ◽  
pp. 652
Author(s):  
Haseena K. Aboobacker ◽  
Gopesh Valoth ◽  
Sabeena Kizhedath
Keyword(s):  
Immune Deficiency ◽  
Antioxidant Activity ◽  
Heart Disease ◽  
Free Radical ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging

Background: Newer drug research worldwide is now focusing on medicinal plants for ensuring health and vitality due to the seemingly safer side effect profile and abundance of plants in nature, compared to synthetic drugs. Antioxidants are vital in preventing free radical induced tissue damage. The purpose of the present study is to evaluate the antioxidant activity of the methanolic extract of the leaves of Carica papaya using 1,1-diphenyl-2-picrylhydrazine (DPPH). Phytochemical tests proved the presence of bioactive ingredients in the extract.Methods: DPPH free radical assay, one of the most accurate methods for evaluating antioxidant activity, was used to evaluate the antioxidant activity of methanolic extract of leaves of C. papaya.Results: The methanolic leaf extract of C. papaya showed antioxidant property with free radical scavenging activity increasing with increase in concentration. The IC50 value of methanolic extract was 213.68 µgm/ml. Ascorbic acid was used as control.Conclusions: Oxidative stress has been linked to heart disease, cancer, immune deficiency. Antioxidants as suggested from various studies may reduce the risk of such diseases. The utility of C. papaya in the treatment of heart disease, cancer and immune deficiency will have to be proved by detailed evaluation of its pharmacological properties.

scholarly journals Phytochemical screening and In-vitro antioxidant activity of Centella asiatica extracts

2017 ◽  
Vol 9 (4) ◽  
pp. 615
Author(s):  
Mukesh Kumar Yadav ◽  
Santosh Kumar Singh ◽  
JS Tripathi ◽  
YB Tripathi
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Centella Asiatica ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Phytochemical Screening

<p><em>Centella asiatica</em> also known as <em>mandukparni </em>or Indian pennywort or <em>jalbrahmi</em>, which has been used as a medicine in the Ayurveda from ancient times and mentioned in many classical texts of Ayurveda. <em>Centella asiatica</em> has long been used to improve memory and cognitive function.</p><p>The study aimed to identify the phytochemicals present in different solvent extracts of <em>Centella asiatica </em>(i.e. PECA- Petroleum ether extract of <em>C. asiatica, </em>CCA- Chloroform extract of <em>C. asiatica, </em>EACA- Ethyl acetate extract of <em>C. asiatica,</em> ECA- Ethanolic extract of <em>C. asiatica, </em>HACA- Hydro-alcoholic extract of <em>C. asiatica</em>)<em> </em>and evaluate the respective in-vitro antioxidant potentials. <em></em></p><p>The phytochemical screening of extracts was done with standardized procedures and the antioxidant potential of different solvent extracts of <em>Centella asiatica</em> was assessed by its free radical scavenging activity 2, 2-diphenyl -1- picrylhydrazyl (DPPH) as well as hydrogen peroxide scavenging assay respectively for reducing capability.</p><p>In all different solvent extracts of <em>C. asiatica</em> revealed excellent free radical scavenging activity as revealed by 2-2- diphenyl-1-picryl-hydrazyl (DPPH) assay with  EC<sub>50</sub> values for ECA=128.752±1.85 μg/ml, HACA=274.884±1.21 μg/ml and hydrogen peroxide assay against the standard (Butylated hydroxytoluene) BHT, with the EC<sub>50</sub> values ECA=429.69±0.92 μg/ml HACA=458.08±0.58 μg/ml while rest solvent extracts shown very less antioxidant activity.</p><p> The present study indicates that the <em>Centella asiatica</em> extracts have good antioxidant activity which can be used in stress and anxiety and also a good source to be used as natural drugs.</p>

scholarly journals Biopolymer from Adenanthera pavonina L. Seeds: Characterization, Photostability, Antioxidant Activity, and Biotoxicity Evaluation

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Roberta Cardoso Melo ◽  
Maurycyo Silva Geronço ◽  
Rayran Walter Ramos Sousa ◽  
Lara Polyana Silva Ramos ◽  
Francisca Pereira Araújo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Acute Toxicity ◽  
Uv Irradiation ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Adenanthera Pavonina ◽  
Pharmaceutical Industries

Plant polysaccharides have been increasingly employed in the pharmaceutical, industrial, and food environments due to their versatile functional properties. In the present investigation, a heteropolysaccharide galactomannan (GAP) was extracted from Adenanthera pavonina L. seeds and characterized by physicochemical analyses to determine its thermal properties, photostability, antioxidant activity, and acute toxicity. GAP was characterized by FTIR, DSC, and TG. The photostability of GAP submitted to artificial UV irradiation was analyzed. Antioxidant activity was evaluated by the DPPH (2,2-diphenyl-1-1-picrylhydrazyl) free radical-scavenging method, while a bioassay method was carried out to study acute toxicity in Artemia salina L. Physical-chemical and functional characteristics of GAP support its potential role in the food and pharmaceutical industries. GAP was photostable under UV irradiation. In vitro GAP antioxidant evaluation showed that it bears free radical-scavenging activity for DPPH radicals. The median lethal concentration (LC50) of GAP was 239.4 mg∙mL−1, indicating that this biopolymer is nontoxic. Such results indicate that this biopolymer presents characteristics of neutrality, photostability, and nontoxicity that are commercially attractive.

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF LEAVES OF CORCHORUS OLITORIUS

2017 ◽  
pp. 59-63
Author(s):  
Abdul Sadat ◽  
Mayukh Hore ◽  
Kaushik Chakraborty ◽  
Subhrajyoti Roy
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Objective: The present study was carried out to evaluate the in vitro preliminary phytochemical analysis and antioxidant activity of methanolic leaf extracts of Corchorus olitorius L. The in vitro antioxidant activity was evaluated by DPPH radical scavenging activity method.Methods: Fresh jute leaves (immature, mature and senescence) were collected, air dried and the crude powder was prepared for phytochemical and antioxidant analysis. The powder was mixed with 70% methanol and the supernatant was separated. The antioxidant activity of this methanolic extract was measured on the basis of the scavenging activity of the stable 1, 1-diphenyl 2-picrylhydrazyl (DPPH) free radical with slight modifications. Phytochemical analysis was performed according to standard laboratory protocol.Results: The results indicated the presence of different phytochemicals viz. glycosides, steroids, cholesterol, alkaloids, phenols, flavonoids, riboflavin, saponins and terpenoids. The sample also showed antioxidant activity by inhibiting DPPH radical. The significant antioxidant activity of methanolic leaf extract of might be due to the presence of saponins, phenols, flavonoids and alkaloids found in the preliminary phytochemical analysis.Conclusion: Present study reveals that the jute leaf possesses different phytonutrients and exhibited DPPH radical scavenging activity, and therefore, may be used for therapeutic purposes.

scholarly journals Synthesis and in vitro Cytotoxicity Study of Novel 4-Substituted Quinazolines Encompassed with Thiazolidinone and Azetidinone

2020 ◽  
Vol 32 (10) ◽  
pp. 2617-2623
Author(s):  
Akash Jori ◽  
Sheshagiri R. Dixit ◽  
Gurubasavraj V. Pujar
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Dna Binding ◽  
Anticancer Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
In Vitro Cytotoxicity ◽  
Free Radical Scavenging ◽  
Binding Studies

A series of quinazolines encompassed with thiazolidinone and azetidinone have been synthesized and evaluated for their antioxidant, anticancer and DNA binding studies. All the synthesized compounds were characterized by IR, 1H & 13C NMR and mass spectra. Antioxidant activity was carried out using % free radical scavenging by DPPH assay. Compounds 4b, 5b and 5d have shown better antioxidant activity (60, 67 and 66%, respectively) among the tested compounds. Compounds having % free radical scavenging activity more than 55% were evaluated for anticancer activity by MTT assay towards cell lines A-549 (lung carcinoma) and MDA-231 (human breast cancer). Results revealed that the tested compounds exhibited moderate to low anticancer activity. Further, DNA binding activity was studied by absorption titration method for all the synthesized compounds, and compound 5b showed a good binding constant of 70.05 and % hyperchromicity of 82.93%.

scholarly journals FREE-RADICAL SCAVENGING ACTIVITY LEAF EXTRACT OF LITSEA LAEVIGATA GAMBLE

2019 ◽  
pp. 96-103
Author(s):  
S. SUJATHA ◽  
T. SEKAR
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Leaf Extract ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Metal Chelating

Objective: In the present study, antioxidant activity in the leaf of the pet-ether, chloroform, acetone and methanolic extracts from Litsea laevigata Gamble. Leaf was investigated by employing established in vitro studies. L. laevigata belongs to the Lauraceae family. Methods: The capability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals were determined by in vitro antioxidant assays using 2,2-diphenyl-2-picrylhydrazyl free radical (DPPH.) scavenging, reducing power assay, superoxide radical (O2*-) scavenging activity, phosphomolybdenum assay, FRAP, ABT and metal chelating activity were performed to know the antioxidant potency of the plant extract of leaves of L. laevigata. Results: Results are evaluated higher in leaf extract of L. laevigata recorded total phenol, total flavonoid, and tannin. The present state of work was designed to evaluate the phytochemical, antioxidant in the plant leaf extracts of L. laevigata. The plant L. laevigata methanolic extract of leaf showed greater IC50 antioxidant activity of DPPH assay (5.264 µg/ml) and compare to other extract, higher phosphomolybdenum reduction (164.36 mg/g), better Reducing power activity leaf in methanol (0.711%), higher ferric reducing power (4060.66MmolFe(II)E/mg), and higher in superoxide radical scavenging activity in (78.12 mg/ml). However, the better metal chelating ability was shown by the water extracts of the leaf (5.145 EDTAE/100g) compared to other solvent extracts. Conclusion: The result indicates the total phenol and antioxidant activity potential of L. laevigata.

scholarly journals Evaluation of antioxidant activity of ormeloxifene: in vitro study

2017 ◽  
Vol 6 (10) ◽  
pp. 2507
Author(s):  
Vinay M. ◽  
Seethalakshmi S. ◽  
Vijay Kumar
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
No Synthase ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Dpph Assay

Background: Ormeloxifene (Centchroman) is a Selective Estrogen Receptor Modulator (SERM) which acts as estrogen antagonist and having anti progestogenic activity also. It is being used in the management of dysfunctional uterine bleeding and as nonhormonal oral contraceptive. It is also being investigated for the indications such as osteoporosis, breast and endometrial carcinoma. In this study, we have evaluated the Antioxidant potential of drug by using DPPH and NO synthase Assay. It was found that ormeloxifene has significant antioxidant activity which could be cause for its use in various gynaecological and other conditions.Methods: In this study, we have demonstrated in vitro antioxidant activity of ormeloxifene. DPPH and NO synthase assay tests were done for different concentrations of ormeloxifene.Results: In our study, it showed that the free radical scavenging activity of ormeloxifene was less in lower concentration and increased in the higher concentration in DPPH assay. The free radical scavenging activity of drug ormeloxifene was 22% at 100µg/ml and 27% for the concentrations of 1000µg/ml in DPPH assay. No scavenging activity was 3% at 100µg/ml and 11% at 1000µg/ml.Conclusions: The invitro antioxidant analysis of ormeloxifene, was proved to be a potent antioxidant.

scholarly journals Phytochemical analysis, mineral composition and in vitro antioxidant activities of Solanum macrocarpon leaves

2016 ◽  
Vol 4 (1) ◽  
pp. 62
Author(s):  
Usunomena Usunobun ◽  
Igwe V. Chinwe
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Phytochemical Screening ◽  
Mineral Analysis

Background: The aim of this study is to determine phytochemicals and mineral composition as well as in vitro antioxidant activities of Solanum macrocarpon leaves.Methods: Qualitative phytochemical screening was carried out using standard procedures while Mineral analysis was carried out using Atomic Absorption Spectrophotometer (AAS). Solanum macrocarpon leaves were also subjected for measurement of reducing power and antioxidant/radical scavenging activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity).Results: Phytochemical screening revealed the presence of flavonoids, saponins, alkaloids etc. Mineral analysis showed calcium (256.60mg/100g) to be higher in concentration and copper (0.62mg/100g) least in concentration while manganese was absent. Other minerals includes magnesium (81.69mg/100g), potassium (87.22mg/100g), sodium (32.51mg/100g), iron (31.41mg/100g), zinc (1.41mg/100g). Solanum macrocarpon leaves showed maximum antioxidant activity (DPPH free radical scavenging and reducing power capacity) as the higher the concentration, the higher the antioxidant activity, thus the better the free radical scavenging potentials.Conclusion: The data from this study revealed that Solanum macrocarpon has a rich content of phytochemicals, namely, saponins, alkaloids, flavonoids as well as minerals, bioactive components that are associated with health impacts. This study also revealed that Solanum macrocarpon leaves exhibit antioxidant activity. These findings thus suggest that Solanum macrocarpon leaves could act as potent source of antioxidants.

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