An intronic polymorphism of the gene Plakophilin-3 is associated with IVF failure

Background: Implantation failure is determined when transferred embryos fail to implant following in vitro fertilization (IVF). In recent years, many studies suggest that implantation failure could be related to several genetic factors. In the current study, authors aimed to investigate the association of PKP3 rs10902158 (G>A) polymorphisms with the risk of implantation failure after ICSI treatment.Methods: 97 women, who underwent ICSI treatment owing to male factor infertility, were prospectively recruited in this cross-sectional study. Genomic DNA was prepared from peripheral blood samples in order to analyze the polymorphism (rs10902158) at the PKP3 gene by PCR-RFLP. The Results were presented as a genotype (GG, GA, and AA), and their relationship to IVF outcome was analyzed.Results: The patients were divided into two groups according to clinical pregnancy: the pregnant group included 51 patients (53%) and the non-pregnant group included 46 patients (47%). The clinical pregnancy outcome was significantly different between genotypes, which was 0%, 45.8% and 58.8% in the patients having the genotype AA, GA and GG respectively (p-value = 0.03).Conclusions: The presence of the allele A of the PKP3 SNP rs10902158 is associated with a reduced clinical pregnancy outcome in the patients undergoing ICSI treatment and may be helpful predictor for implantation failure.

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Impact of thawing-loading interval of frozen embryo on pregnancy outcome: retrospective analysis

Voprosy ginekologii akušerstva i perinatologii ◽

10.20953/1726-1678-2021-5-5-11 ◽

2021 ◽

Vol 20 (5) ◽

pp. 5-11

Author(s):

A.M. Abbas ◽

◽

A.H.A. Hakim ◽

H.A. Bayoumy ◽

W.M. Abuelghar ◽

...

Keyword(s):

In Vitro Fertilization ◽

Pregnancy Outcome ◽

Embryo Transfer ◽

Odds Ratio ◽

Adjusted Odds Ratio ◽

Clinical Pregnancy ◽

The Other ◽

P Value ◽

Vitro Fertilization

Embryo transfer is a critical final step in the in vitro fertilization (IVF) cycle. Much attention has been paid to standardizing the procedures of embryo transfer to improve pregnancy outcome. Objective. To identify the impact of embryo thawing-loading interval (the time interval passing from thawing to loading of frozen embryo into the catheter) on pregnancy outcome. Results. The study was divided into quartiles according to thawing-loading interval in minutes (G I: <60; G II: 60–119; G III: 120–239; G IV: 240 and above). Numerical variables are presented as median and interquartile range, and inter-group differences are compared using the Mann-Whitney U test. Categorical variables are presented as numbers and percentages, and differences are compared using the chi-square test for trend. After adjustment for the age of women, time of previous failed ICSI (Intracytoplasmic sperm injection) trials, number and maturity of transferred embryos, a thawing-loading interval between 60–119 minutes (adjusted odds ratio = 2.222, 95% CI = 1.370 to 3.603, p-value = 0.001) or between 120–239 minutes (adjusted odds ratio = 1.924, 95% CI = 1.172 to 3.161, p-value = 0.010) was independently associated with increased probability of clinical pregnancy compared with a thawing-loading interval of <60 minutes. On the other hand, a thawing-loading interval of ≥240 minutes significantly decreased the probability of clinical pregnancy (adjusted odds ratio = 0.378, 95% CI 0.215 to 0.664, p-value = 0.001). Conclusion. After adjusting for potential confounders, this analysis found that a thawing-loading interval between 60–239 minutes was independently associated with better pregnancy outcomes compared with a thawing-loading interval of <60 minutes. On the other hand, a thawing-loading interval of ≥240 minutes was significantly associated with a worse outcome. Key words: embryo transfer, IVF (in vitro fertilization), frozen embryo, thawing-loading interval

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The Association Between Serum Estradiol and Progesterone On the Same Day of FET and The Pregnancy Outcome; A Cross Sectional Study

International Journal of Women’s Health Care ◽

10.33140/ijwhc.06.02.03 ◽

2021 ◽

Vol 6 (2) ◽

Keyword(s):

Pregnancy Rate ◽

Pregnancy Outcome ◽

Endometrial Thickness ◽

Research Question ◽

Cross Sectional Study ◽

Clinical Pregnancy ◽

P Value ◽

Sectional Study ◽

Cross Sectional ◽

The Impact

Research Question Precise timed synchronization between endometrium and the embryo is essential for high implantation and pregnancy rate, it is worthy to mention that endometrial thickness is not the only factor, E2 and P levels are also regularly monitored for endometrial receptivity. So, we decided to go for this study, to investigate the impact of serum E2 and P levels on the same day of embryo transfer on pregnancy outcomes for FET cycles. Design This was a retrospective cross sectional study for 402 FET cycles which conducted between April 2018 and May 2019. All participants started endometrial preparation for FET with 6 mg/day oral estradiol for 13 days. When endometrium reached 8 mm or greater, patients were initiated on both micronized vaginal and oral P treatment. On FET day, serum level of E2 and P were assessed. Then, transfer of PGT euploid embryos was performed. 12 days’ later pregnancy test was assessed, and then 4 weeks after FET date ultrasound was scheduled to check the viability and the clinical pregnancy. Results The mean E2 value was 931.41 ± 438.65 pg/ml, while mean P value was 8.47 ± 9.4 ng/ml. 240 out of 402 cases got pregnant (59.7%) while the clinical pregnancy rate was 53.9% with no correlation between serum (E2, P & E/P ratio) and the outcome. Conclusion Our results revealed that the association between E2 and P on FET day and the pregnancy outcome is still not proven and those markers can’t serve as predictors for the outcome.

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An Evaluation of the Use of G-CSF as an Adjunct to IVF in Women Who Have Previously Failed Attempts at Pregnancy With IVF

International Journal of Women s Health and Reproduction Sciences ◽

10.15296/ijwhr.2019.45 ◽

2018 ◽

Vol 7 (3) ◽

pp. 269-275

Author(s):

Tasneem Mohamed ◽

Yasmin Adam ◽

Mohamed Iqbal Cassim

Keyword(s):

Pregnancy Outcome ◽

Endometrial Thickness ◽

Univariate Analysis ◽

Cross Sectional Study ◽

Age Difference ◽

Cross Sectional ◽

Vitro Fertilization ◽

Younger Women ◽

The Mean

Objectives: The present study aimed to evaluate the effects of granulocyte colony-stimulating factor (G-CSF) as an adjunct to in vitro fertilization (IVF). In other words, it delved into the influence of G-CSF on the endometrium and the achievement of pregnancy. Materials and Methods: This retrospective cross-sectional study was conducted on a subgroup of women with two or more previously failed IVFs, who attended a fertility clinic in Johannesburg. These women underwent a procedure of transvaginal infusion of G-CSF in addition to their IVF protocol although endometrial thickness was not a criterion for G-CSF use. Results: The group included 49 women with a mean age of 38.9 years (SD ± 6.11). The mean number of previous IVFs was 3.1 (SD ± 1.76). The mean endometrial thickness pre-G-CSF and post-G-CSF was 7.53 mm (SD ± 2.69) and 9.11 mm (SD ± 2.12), respectively. The clinical pregnancy rate was 34.69%. Univariate analysis between the groups of women who achieved or failed to achieve pregnancy showed that the age difference between the two groups was statistically significant (P=0.0005). Further, G-CSF use was associated with increased pregnancy rates in younger women. Finally, although the mean endometrial thickness pre and post-G-CSF were not statistically significant between the two groups (P>0.05), the mean change in endometrial thickness was statistically significant in all women regardless of pregnancy outcome (P=0.0029). Conclusions: In general, G-CSF is considered as a useful adjunct for the treatment of women with recurrent failed IVFs and aged less than 38 years. Based on the findings, a statistically significant overall expansion of endometrial thickness was reported by using G-CSF. However, it failed to show any association between endometrial expansion and pregnancy outcome.

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Sirtuin 1 Levels in Recurrent Implantation Failure

Revista Brasileira de Ginecologia e Obstetrícia / RBGO Gynecology and Obstetrics ◽

10.1055/s-0037-1606349 ◽

2017 ◽

Vol 39 (10) ◽

pp. 541-544 ◽

Cited By ~ 1

Author(s):

Yaprak Engin-Ustun ◽

A. Ozgu-Erdinc ◽

Emel Kiyak Caglayan ◽

Cavidan Gulerman ◽

Esma Sarikaya ◽

...

Keyword(s):

Pregnant Women ◽

Statistical Significance ◽

Cross Sectional Study ◽

Sirtuin 1 ◽

Implantation Failure ◽

Recurrent Implantation Failure ◽

Cross Sectional ◽

Vitro Fertilization ◽

Number Of Patients

AbstractSirtuin 1 has an important role in cellular processes, including apoptosis and cellular stress. The purpose of this study was to assess serum sirtuin 1 levels in women with recurrent implantation failure (RIF). In this cross-sectional study, we included 28 women with RIF, 29 healthy women who had conceived by in vitro fertilization (IVF), and 30 women with a 1-cycle failure of IVF as controls. Human serum nicotinamide adenine dinucleotide (NAD)-dependent deacetylase sirtuin-1 (SIRT1/SIRT2L1) levels were detected using a commercial colorimetric kit. Recurrent implantation failure patients have higher sirtuin 1 levels than non-pregnant women and healthy pregnant women, but this difference did not reach statistical significance due to the low number of patients in our study. These higher sirtuin 1 levels may result from the inflammation imbalance of RIF patients. The only statistically significant correlation found was between age and sirtuin (r = 0.277, p = 0.009).

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Analysis of Oocyte and Embryo Numbers, and Pregnancy Rate in Patients Undergone In Vitro Fertilization with Long and Short Protocols

Indonesian Journal of Obstetrics and Gynecology ◽

10.32771/inajog.v36i2.294 ◽

2012 ◽

pp. 95-99

Author(s):

Nadia Shafira

Keyword(s):

In Vitro Fertilization ◽

Pregnancy Rate ◽

Cross Sectional Study ◽

Current Data ◽

Male Factor ◽

Cross Sectional ◽

Vitro Fertilization ◽

Tubal Factor ◽

Long Protocol

Objectives: To compare the oocyte count, embryo count, pregnancy rate, and the presence of OHSS in patients undergoing In Vitro Fertilization (IVF) with long and short protocols at Yasmin Clinic, Dr. Cipto Mangunkusumo Hospital, Jakarta. Methods: A cross sectional study was done at Yasmin Reproductive Clinic, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia. Data was collected from the medical records of 200 infertile women undergoing IVF, 100 women with long protocols and the other 100 with short protocols. Variables compared in these 2 groups are the oocyte count, embryo count, the pregnancy rate, and presence OHSS using Mann-Whitney U Test with SPSS 11 Program. Result: From 200 women undergoing IVF procedure, 45.5% of them aged between 35-40 years old, with the youngest is 22 years old and the oldest is 48 years old (average age=35 years, SD=4.7), and almost half of them (45%) have BMI between 18.01-23.00 kg/m2. 185 women has primary infertility. These subjects came to the clinic mostly due to male factor (23.5%), idiopathic cause (19%), and ovulation disturbance (13%), whilst endometriosis, tubal factor, and other factors take just little percentage. When compared between long and short protocols, the number of oocyte retrieved is significantly different (p=0.007, CI=5.84-7.11), whereas embryo numbers (p=0.054, CI=1.80-2.39) and the pregnancy rate (p=0.525, CI=0.21-0.33) found to be not significantly different. There were 2 cases of moderate OHSS developed in the long protocol group. Conclusion: Although the number of embryo produced and the pregnancy rate are found to be not significantly different between the two groups, long protocol of IVF is found to produce more oocyte to be retrieved and develop more OHSS events compared to short protocol. Due to this result, GnRH antagonist can be considered when planning an IVF procedure. However, a longitudinal multicenter study with larger sample size is needed to validate the current data. [Indones J Obstet Gynecol 2012; 36-2: 95-9] Keywords: in vitro fertilization, long protocol, pregnancy rate, short protocol

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Results and experiences after 1000 IVF cycles at the Clinic for gynecology and obstetrics in Novi Sad

Medicinski pregled ◽

10.2298/mpns1112565k ◽

2011 ◽

Vol 64 (11-12) ◽

pp. 565-569

Author(s):

Vesna Kopitovic ◽

Stevan Milatovic ◽

Aleksandra Trninic-Pjevic ◽

Artur Bjelica ◽

Irena Bujas ◽

...

Keyword(s):

In Vitro Fertilization ◽

Intracytoplasmic Sperm Injection ◽

Clinical Pregnancy ◽

Reproductive Age ◽

Male Factor ◽

Fertilization Program ◽

Vitro Fertilization ◽

Gynecology And Obstetrics ◽

Novi Sad

Introduction. Infertility affects 15-17% of reproductive age couples in our country, and 10-15% of couples worldwide. The aim of this paper was to present results and experience gained after the first 1000 cycles of the national In Vitro Fertilization (IVF) program, to offer professional standard of work in our country and to compare it with results obtained in Europe and worldwide. Material and methods. The study prospectively included 1000 women who had undergone national In Vitro Fertilization program from October 2006 until November 2009 at the Department of Gynecology and Obstetrics, Clinical Center of Vojvodina, Novi Sad. Results. The analysis included 1000 in vitro fertilization cycles. Male factor infertility was the leading cause (56.9%) followed by tubal factor (45.9%). The classic method of in vitro fertilization constituted 72.3% of all cycles, while intracytoplasmic sperm injection (ICSI) method was used in 27.7% of all cycles. The average number of embryos transferred was 2.67. The cycle cancellation rate was 14.10% and the aspiration rate was 94.40%. The clinical pregnancy and live birth rate were 33.41% and 26.78% per embryo transfer, respectively. Discussion and conclusion. The results in our study showed that in our setting there are far less intracytoplasmic sperm injection cycles compared with the European average of 66.5% of all fresh cycles, and that we transferred more embryos on average. Our success rates are comparable with those in other European countries where the clinical pregnancy rates per aspiration and per transfer for in vitro fertilization were 29 and 32.4%, respectively in the period of observation. For intracytoplasmic sperm injection, the corresponding rates were 29.9 and 33%.

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Transferrin and antioxidants partly prevented mouse oocyte oxidative damage induced by exposure of cumulus-oocyte complexes to endometrioma fluid

Journal of Ovarian Research ◽

10.1186/s13048-020-00738-0 ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Zi Ren ◽

Jiana Huang ◽

Chuanchuan Zhou ◽

Lei Jia ◽

Manchao Li ◽

...

Keyword(s):

Oxidative Damage ◽

Oocyte Retrieval ◽

Cross Sectional Study ◽

Human Oocyte ◽

Cross Sectional ◽

Vitro Fertilization ◽

Good Quality Embryo ◽

Damage Process ◽

Human And Mouse

Abstract Background Exposure of oocytes to the endometrioma fluid has an adverse effect on embryonic quality. To determine whether adding transferrin and antioxidants to culture medium could counteract detrimental effects on mouse cumulus-oocyte complexes (COCs) induced by exposure to endometrioma fluid or not, we conducted an in vitro cross-sectional study using human and mouse COCs. Methods Eighteen women who had their oocytes exposed to endometrioma fluid during oocyte retrieval were enrolled. COCs from superovulated ICR female mice were collected. They were first exposed to human endometrioma fluid and then treated by transferrin and/or antioxidants (cysteamine + cystine). Subsequently, COCs function was assessed by molecular methods. Results This study observed that human COCs inadvertently exposed to endometrioma fluid in the in vitro fertilization (IVF) group led to a lower good quality embryo rate compared to intracytoplasmic sperm injection (ICSI) group. Exposure of mouse COCs to endometrioma fluid accelerated oocyte oxidative damage, evidenced by significantly reduced CCs viability, defective mitochondrial function, decreased GSH content and increased ROS level, associated with the significantly higher pro-portion of abnormal spindles and lower blastocyst formation (p < 0.05, respectively). This damage could be recovered partly by treating COCs with transferrin and antioxidants (cysteamine + cystine). Conclusions Transferrin and antioxidants could reduce the oxidative damage caused by COCs exposure to endometrioma fluid. This finding provides a promising new possibility for intervention in the human oocyte oxidative damage process induced by endometrioma fluid during oocyte pick-up.

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Effect of large follicle puncture on IVF-ET outcome in patients with unsynchronized follicle maturationcan

Journal of International Medical Research ◽

10.1177/0300060519831178 ◽

2019 ◽

Vol 47 (5) ◽

pp. 2056-2066

Author(s):

Xinrong Wang ◽

Wenjuan Wang ◽

Qinglan Qu ◽

Ning Zhang ◽

Cuifang Hao ◽

...

Keyword(s):

Pregnancy Outcome ◽

Pregnancy Outcomes ◽

Endometrial Thickness ◽

Multivariate Logistic Regression Analysis ◽

Clinical Pregnancy ◽

Control Group ◽

Large Follicle ◽

Vitro Fertilization ◽

Follicular Maturation

Objective This retrospective study was conducted to explore causes of unsynchronized follicular maturation (UFM) and analyze the effects of large follicle puncture on embryo quality and pregnancy outcome. Methods Clinical features and controlled ovulation hyperstimulation (COH) were compared between the puncture group (n = 48) and the control group (n = 2545). We analyzed the COH process with in vitro fertilization during fresh cycle embryo transfer with different clinical pregnancy outcomes. We compared clinical characteristics and COH process of patients in the clinical pregnancy (n = 774) and non-clinical pregnancy (n = 527) groups. Finally, factors related to pregnancy outcomes were analyzed using multivariate logistic regression analysis. Results Age, level of estradiol on down-regulation day, and initial gonadotropin dose were significantly higher in the puncture group than in the control group. We detected significant differences in age, infertility, and body mass index (BMI) between the clinical and non-clinical pregnancy groups. Age, BMI, and endometrial thickness on the day of human chorionic gonadotropin administration were the independent factors influencing pregnancy outcome. Conclusions Patient’s age and level of anti-Müllerian hormone were the main factors causing UFM in patients undergoing COH. Large follicle puncture had no significant effect on pregnancy outcome.

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