scholarly journals Pattern of extra pulmonary tuberculosis among urban population with special reference to CBNAAT as a diagnostic tool: a retrospective study at a tertiary care hospital of Odisha, India

2018 ◽  
Vol 6 (10) ◽  
pp. 3375
Author(s):  
C. Mohan Rao ◽  
Bhaskar Thakur ◽  
Saswat Subhankar ◽  
D. P. Dash
Keyword(s):  
Lymph Node ◽  
Tertiary Care Hospital ◽  
Diagnostic Yield ◽  
Point Of Care ◽  
Tertiary Care ◽  
Pulmonary Involvement ◽  
The Body ◽  
Nucleic Acid Amplification ◽  
Care Hospital ◽  
Extra Pulmonary

Background: Diagnosis of Extra-pulmonary TB (EPTB) is a challenge. Authors wanted to assess the sites of extra-pulmonary involvement during 2013-2017 in a tertiary care hospital cum medical college. Authors also wanted to evaluate the role of Cartridge Based Nucleic Acid Amplification Test (CBNAAT) in diagnosis of EPTB and compare its efficacy with AFB Culture.Methods: Total 470 EPTB cases diagnosed between 2013 and 2017 from 840 TB treatment records maintained in designated microscopy centre. Specific samples from appropriate sites were taken up for smear for AFB, CBNAAT and AFB culture.Results: There was incremental detection and registration in both TB and EPTB cases from 96 and 50 cases in 2013 to 246 and 150 cases in 2017 respectively. Among the total 470 EPTB cases in 2013-2017 (55.9%), lymph node followed by pleura and abdomen were the organs having maximum involvement. Bone involvement was more witnessed in adult male than children (p <0.05). There was male preponderance. CBNAAT results were 100 % sensitive and 87.5% specific. Lymph node samples and pus elsewhere in the body had much better diagnostic yield than serous effusions.Conclusions: Awareness and availability of diagnostic services in tertiary care institutions has lead to increased reporting of EPTB under RNTCP services. CBNAAT can be also be utilized as a point of care testing for lymph node aspirate and pus specimen.

Correlation of COVID-19 antigen test and CBNAAT results at a single tertiary care hospital in North India: an experience

2021 ◽  
Vol 8 (5) ◽  
pp. 2427
Author(s):  
Surbhi Gupta ◽  
Anju Shukla ◽  
Poonam Singh ◽  
Areena H. Siddiqui
Keyword(s):  
Tertiary Care Hospital ◽  
Point Of Care ◽  
Tertiary Care ◽  
Uttar Pradesh ◽  
Nucleic Acid Amplification ◽  
North India ◽  
Care Hospital ◽  
Antigen Test ◽  
Discordant Group ◽  
Antigen Tests

Background: Nucleic acid amplification test (NAAT) is considered gold standard in the molecular diagnosis of CoV-2 infection but since it is costly, labor intensive and needs technical expertise, rapid chromatographic immunoassay for the qualitative detection of specific antigens to SARS CoV-2 have been devised. Objectives of this study was to compare the results of Antigen test and NAAT for CoV-2 infection carried out during the months of July and August 2020 by single tertiary care hospital in Lucknow, Uttar Pradesh and to determine the utility of rapid antigen test in the SARS CoV-2 diagnosis.Methods: All the patients who came to our hospital seeking admission during July 2020 and August 2020 were included in the study. A total of 1000 patients were included in this study.Results: Out of a total 1000 cases which were included in the study, 769 cases (76.9%) were found to be SARS CoV-2 negative by both antigen and CBNAAT, 100 cases (10.0%) were SARS CoV-2 positive by both antigen and CBNAAT tests. But in 131 cases (13.1%), antigen was not able to pick up the disease. It was also found that the Cycle Threshold (Ct) value for the discordant group was higher (Mean E= 28, Mean N2=33) when compared to the group where antigen was positive.Conclusions: The present study establishes the role of rapid antigen tests in contributing to the quick, point of care diagnosis of SARS CoV-2. These assays are safe, simple, and fast and can be used in local clinics and hospitals. These tests are very important for real-time patient management and infection control decision.

A STUDY ON EXTRA PULMONARY TUBERCULOSIS: SITE DISTRIBUTION

2021 ◽  
pp. 25-26
Author(s):  
Chhotala Yagnik ◽  
Vishal Oza ◽  
Chetal Suva
Keyword(s):  
Lymph Node ◽  
Pulmonary Tuberculosis ◽  
Tertiary Care Hospital ◽  
Extrapulmonary Tuberculosis ◽  
Tertiary Care ◽  
Abdominal Tuberculosis ◽  
Care Hospital ◽  
Extra Pulmonary Tuberculosis ◽  
Site Distribution ◽  
Extra Pulmonary

Background:Extra-pulmonary tuberculosis can affect the lymph nodes, pleura, bones, joints, genito-urinary tract, nervous system (meningitis, tuberculoma), abdominal tuberculosis (intestines, mesentery, solid organs), skin. Materials and Methods: It is a record based, observational prospective study which assesses the presentation and evaluation of the site of distribution of extrapulmonary tuberculosis and management of it in a tertiary care hospital. Result:In present study, 26% of cases from lymph node TB, 20% cases from CNS and pleural TB, 18% cases from GIT, 8% cases from skeletal TB, 4% cases from GUT, 2% cases from ocular and 2% cases from pericardial TB Conclusion:The frequency of extrapulmonary TB in this study was highest in lymph node followed by pleural, CNS, GIT, skeletal and others respectively

scholarly journals DIAGNOSTIC PERFORMANCE OF ZIEHL-NEELSEN & CBNAAT TECHNIQUES IN PULMONARY TUBERCULOSIS, A RETROSPECTIVE ANALYSIS AT TERTIARY CARE HOSPITAL IN JHALAWAR, RAJASTHAN.

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Shilpi Hora ◽  
Neeraj Raman ◽  
Mukesh Kumar ◽  
Needa Saneef
Keyword(s):  
Pulmonary Tuberculosis ◽  
Tertiary Care Hospital ◽  
Point Of Care ◽  
Tertiary Care ◽  
Nucleic Acid Amplification ◽  
Smear Microscopy ◽  
Care Hospital ◽  
Age Group ◽  
Hands On ◽  
High Incidence

Introduction: Tuberculosis (TB) remains a leading cause of death among infectious diseases worldwide. The advent of the CBNAAT was a revolution in the diagnosis of tuberculosis, especially with high incidence and resource poor regions. It can be used close to the point of care by operators with minimal technical expertise, enabling diagnosis of TB and rifampicin Resistance (RIF) concurrently using unprocessed clinical specimens, in less than 2h. Aim: To evaluate the diagnostic performances of Ziehl-Neelsen & CBNAAT techniques in detection of Pulmonary Tuberculosis. Materials and Methods: We retrospectively reviewed the 2414 sputum samples of suspected pulmonary TB. Sputum samples were subjected for ZN staining and CBNAAT.RIF resistance was detected by CBNAAT. Results: Out of 2414 samples, 751 sputum samples were positive by smear microscopy. 1127 Samples were confirmed positive by CBNAAT examination. Majority of cases, 43.57% were in 21-40 yrs age group.24.40% were females and 75.59% were males. Sensitivity of CBNAAT was 100% for sputum positive cases and sensitivity was 22.6% for sputum negative cases. Overall RIF resistance was detected in 49 (4.4%) cases in present study. Conclusion: CBNAAT provide sensitive detection of tuberculosis and rifampicin resistance directly from untreated sputum in less than 2 hours with minimal hands-on time. Keywords: Cartridge-based nucleic acid amplification tests (CBNAAT), Rifampicin(RIF)

Cartridge-based nucleic acid amplification test: a novel rapid diagnostic tool to study the burden of tuberculosis from a tertiary care hospital

2019 ◽  
Vol 49 (4) ◽  
pp. 274-281 ◽  
Author(s):  
Bineeta Kashyap ◽  
Nisha Goyal ◽  
Puneeta Hyanki ◽  
NP Singh ◽  
Ashwani Khanna
Keyword(s):  
Nucleic Acid ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Polymerase Chain Reaction Test ◽  
Rifampicin Resistance ◽  
People Living With Hiv ◽  
Care Hospital ◽  
Extra Pulmonary

Despite efforts to limit the morbidity and mortality from tuberculosis (TB), it continues to be an important cause of death. There is an urgent need for a diagnostic test that accurately and quickly diagnoses TB, especially if it is also a near-point-of-care test. The GeneXpert polymerase chain reaction test (known in India as CBNAAT [cartridge-based nucleic acid amplification test] and is capable of diagnosing TB and rifampicin resistance within 2 h) is a promising tool. The duration of our study was two years and was carried out in the DOTS centre of a tertiary care hospital in India. A total of 5449 samples were processed using CBNAAT. Of the total samples tested, 2068 were extra-pulmonary. The following information was collected: number of extra-pulmonary samples processed; number of Mycobacterium tuberculosis ( M. tuberculosis)-positive samples; patterns of rifampicin sensitivity; number of people living with HIV (PLHIV); and number of children. Of the samples, 62.1% were from suspected pulmonary TB patients. Out of the total samples tested using CBNAAT, 21.8% were positive for M. tuberculosis. Rifampicin resistance was seen in 9.2%, 8.5% and 10.3% of the total, pulmonary and extra-pulmonary samples, respectively, in M. tuberculosis-positive samples. Overall, 36.9% samples were from the paediatric population and 5.7% belonged to PLHIV. Rifampicin resistance was seen in 8.8% and 8.3% of the M. tuberculosis-positive paediatric and PLHIV samples, respectively.

Diagnostic yield of Archer FusionPlex PanHeme and solid panels: two-year experience from a pediatric tertiary care hospital

2021 ◽  
Vol 132 ◽  
pp. S39-S40
Author(s):  
Kathleen Schieffer ◽  
Eileen Stonerock ◽  
Vijayakumar Jayaraman ◽  
Heather Jenkins ◽  
Tim Peterson ◽  
...  
Keyword(s):  
Tertiary Care Hospital ◽  
Diagnostic Yield ◽  
Tertiary Care ◽  
Care Hospital

A CLINICOEPIDEMIOLOGICAL STUDY ON PAEDERUS DERMATITIS IN A TERTIARY CARE CENTRE, CHENNAI

2021 ◽  
pp. 25-27
Author(s):  
P. Mohan ◽  
P. Revathi Guru ◽  
V. Sudha ◽  
L. Balamurugan ◽  
Pavithra Gunasekaran ◽  
...  
Keyword(s):  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Sudden Onset ◽  
The Body ◽  
Care Hospital ◽  
Age Group ◽  
Common Condition ◽  
Tertiary Care Centre ◽  
Linear Lesions ◽  
Paederus Dermatitis

Paederus dermatitis is a form of irritant contact dermatitis due to contact with certain insects of the genus Paederus presenting with mostly erythematous linear lesions of sudden onset on exposed parts of the body. To study the clinico epi Aim: demiological features in patients with Paederus dermatitis. A retrospective analysis of clinicoepidemiological Materials & Methods: features of patients affected with Paederus dermatitis who had attended the Dermatology OPD, in a tertiary care hospital between January 2018 to December 2019 was done. All the required data had been collected from the OPD records and details were tabulated and analysed. A total of 56 patients had been affected with Result: Paederus dermatitis during the study period, of which 32 were males and 24 were females. 41out of 56 (73.21%) of patients presented between the months of August and December. Most common age group affected were between 11 to 30 yrs, with front and back of neck being the most common affected sites. In 42 out of the 56 patients the lesions resolved with post inammatory hyperpigmentation. Conclusion: Paederus dermatitis is a common condition that is often misdiagnosed and hence awareness is required for the clinicians and the general public to prevent it.

Prevalence of Extra-pulmonary Granulomatous Lesions in a Tertiary Care Hospital

2021 ◽  
Vol 23 (23) ◽  
Author(s):  
Nihmath Nisha ◽  
Padmapriya B S ◽  
Volga H ◽  
Chitra S
Keyword(s):  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Care Hospital ◽  
Granulomatous Lesions ◽  
Extra Pulmonary

scholarly journals Utility of GeneXpert in Diagnosis of Multidrug-resistant Extrapulmonary Tuberculosis

2017 ◽  
Vol 03 (02) ◽  
pp. 085-087
Author(s):  
Ankush Chaudhary ◽  
Ketaki Utpat ◽  
Unnati Desai ◽  
Jyotsna Joshi
Keyword(s):  
Lymph Node ◽  
Psoas Abscess ◽  
Extrapulmonary Tuberculosis ◽  
Tertiary Care ◽  
False Negative ◽  
Multidrug Resistant ◽  
Nucleic Acid Amplification ◽  
Lower Sensitivity ◽  
Care Hospital ◽  
Gluteal Abscess

ABSTRACT Background: The Xpert Mycobacterium tuberculosis (MTB)/rifampicin (RIF) assay (GeneXpert) is a rapid semi-quantitative nucleic acid amplification test with established role in the diagnosis of pulmonary tuberculosis (PTB) and multidrug-resistant (MDR) PTB. We determined the performance of the GeneXpert assay for the diagnosis of extrapulmonary tuberculosis (EPTB) MDR cases. Aims and objectives: To study the role of GeneXpert in the diagnosis of EPTB MDR. Materials and methods: A retrospective study was conducted over a period of 2 years at a tertiary care hospital after Ethics Committee permission. Data of 44 consecutive patients of diagnosed EPTB MDR were retrieved for GeneXpert and culture drug susceptibility test (DST). Sensitivity of GeneXpert in the diagnosis of EPTB MDR was calculated comparing culture DST results. Results: Various EPTB MDR cases studied were lymph node TB (n = 23) 51%, pleural effusion (n = 14) 32%, central nervous system TB/spinal TB/psoas abscess/gluteal abscess (n = 7) 17%. Sensitivity for GeneXpert was found to be 91.30, 57, 100% respectively. True positive and false negative were 36 and 8 cases respectively. The overall sensitivity of GeneXpert in diagnosing EPTB MDR was 81.80%. The sensitivity among lymph node, pleural effusions, and spinal/psoas abscess/gluteal abscess was 91.30, 57.14, and 100% respectively. Conclusion: GeneXpert sensitivity for the diagnosis of EPTB MDR varied with site of extrapulmonary involvement, with lower sensitivity in pleural fluid as compared with higher sensitivity among lymph node and spine TB. Nevertheless, given the rapid turnaround time and simplicity, it is a useful tool in the diagnosis of EPTB MDR when used in correct clinical context. Subsequent confirmation with culture DST, however, is recommended to diagnose false negatives.

Sign in / Sign up

Export Citation Format

Share Document