To Study the Clinical Profile of Neonatal Sepsis and the Sensitivity of Various Markers of Sepsis Screen

2014 ◽  
Vol 1 (1) ◽  
pp. 19
Author(s):  
Sagar Sonawane ◽  
Milind Suryawanshi ◽  
Priyanka Patil ◽  
Ravindra Sonawane ◽  
M. K. Tolani
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Neonatal Sepsis ◽  
Clinical Manifestations ◽  
Clinical Profile ◽  
Gastric Bleeding ◽  
Automated Method ◽  
Feed Intolerance ◽  
Sepsis Screen ◽  
A Prospective Study

<strong>Objective:</strong> To study the clinical profile of Neonatal Sepsis &amp; the sensitivity of various markers of sepsis screen. <strong>Material &amp; Methods:</strong> This was a prospective study of neonates admitted to our NICU from January 2010 to October 2011 with diagnosis of neonatal sepsis or those who developed sepsis later on during their stay in NICU. All newborns diagnosed as a case of neonatal sepsis, based on clinical features with positive sepsis screen and/or positive blood culture, were included in our study. Blood Culture &amp; Sensitivity was done with conventional non–automated method using Herley’s Broth. <strong>Result:</strong> Common clinical manifestations of Neonatal Sepsis among the study group were Lethargy (96.36%), Tachypnea (92.73%), Refusal to suck/feeding difficulty (76.36%), Delayed CRT, Poor Pulses (74.55%), Sclerema (61.82%), Gastric Bleeding (45.45%) &amp; Feed Intolerance (45.45%). 46 babies had positive sepsis screen (sensitivity 84%), while 27 babies had a positive blood culture (sensitivity 49.09%).

scholarly journals Comparison between sepsis markers and blood culture in diagnosis of neonatal sepsis: a prospective study

2017 ◽  
Vol 5 (4) ◽  
pp. 1662
Author(s):  
Vijay Baburao Sonawane ◽  
Nitin Mehkarkar ◽  
Sonali Gaikwad ◽  
Nitin Kadam
Keyword(s):  
Blood Culture ◽  
Neonatal Sepsis ◽  
Gold Standard ◽  
Predictive Accuracy ◽  
Statistical Correlation ◽  
Diagnostic Process ◽  
Mortality And Morbidity ◽  
Sepsis Screen ◽  
A Prospective Study ◽  
Sepsis Detection

Background: Neonatal septicaemia is one of the commonest causes of neonatal mortality and morbidity. Accurate and timely diagnosis of neonatal sepsis remains a major challenge to the pediatricians and neonatologists. In the present study, correlation between sepsis screening and blood culture in neonate presenting with features of sepsis is done to accelerate the diagnostic process and blood culture (considered gold standard) was evaluated as marker for sepsis detection and its effectiveness was compared with other septic markers.Methods: In present study, we emphasize to study early indicators of sepsis screen and their statistical correlation with blood culture (considered as gold standard).Results: As any sepsis screen parameters showed little correlation with blood culture, yet on combination it was found that specificity and positive predictive accuracy increased while sensitivity decreased them individual tests. Also combination of tests yield better results than single tests.Conclusions: The combination of sepsis makers yielded diagnostic results than single tests and proved to be an invaluable aid for early diagnosis of neonatal sepsis.

scholarly journals To Study Bacteriological Profile and Antibiotic Sensitivity Pattern in Cases of Neonatal Sepsis

2015 ◽  
Vol 2 (1) ◽  
pp. 20
Author(s):  
Sagar Sonawane ◽  
M. K. Tolani ◽  
Milind Suryawanshi ◽  
Priyanka Patil
Keyword(s):  
Blood Culture ◽  
Neonatal Sepsis ◽  
Neonatal Intensive Care ◽  
Late Onset ◽  
Antibiotic Sensitivity ◽  
Automated Method ◽  
Bacteriological Profile ◽  
Late Onset Sepsis ◽  
Sensitivity Pattern ◽  
Sepsis Screen

<strong>Objective:</strong> To study the bacteriological profile and sensitivity pattern in cases of Neonatal sepsis at 12 bedded Neonatal Intensive Care Unit (NICU). <strong>Methods:</strong> This was a prospective study of neonates admitted to our NICU from Jan 2010 to Oct 2011 with diagnosis of neonatal sepsis or those who developed sepsis later during their stay in NICU. All newborns diagnosed as a case of neonatal sepsis, based on clinical features with positive sepsis screen and/or positive blood culture, were included in our study. Blood Culture&amp;Sensitivity was done with conventional non-automated method using Herley's Broth. <strong>Results:</strong> Out of 55 cases blood culture was positive in 27 (49.09%) cases. Klebsiella Pneumoniae 15 (55.55%) was the most common organism isolated in both early and late onset sepsis showed sensitivity to collistin in 86.6% of cases followed by sensitivity to imipenam-cilastin in 46.67% cases. Only 20% Klebsiella isolates were sensitive to drugs like Ampicillin-sulbactum, Amikacin, Tazobactum, cefpime and for other antibiotics like Meropenam, Piperacillin-tazobactum, Vancomycin it was less than 20%. Other organisms isolated were Staph. aureus, E. Coli, Pseudomonas, Enterobactor, Acinetobactor and candida species. <strong>Conclusion:</strong> Gram negative organisms are most common cause of early as well as late onset sepsis and there is alarming degree of antibiotic resistance to commonly used antibiotics.

scholarly journals Predictors of positive blood culture and deaths among neonates with suspected neonatal sepsis in a tertiary hospital, Mwanza- Tanzania

2010 ◽  
Vol 10 (1) ◽  
Author(s):  
Neema Kayange ◽  
Erasmus Kamugisha ◽  
Damas L Mwizamholya ◽  
Seni Jeremiah ◽  
Stephen E Mshana
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Neonatal Sepsis ◽  
Tertiary Hospital

scholarly journals Utility of Red Cell Distribution Width (RDW) In Diagnosis and Prognosis of Early-Onset Neonatal Sepsis in Term Neonates

2021 ◽  
Vol 3 (3) ◽  
pp. 257-264
Author(s):  
Sandeep Golhar ◽  
Abhishek Madhura ◽  
Urmila Chauhan ◽  
Abinash Nayak
Keyword(s):  
Blood Culture ◽  
Hospital Mortality ◽  
Positive Blood Culture ◽  
Neonatal Sepsis ◽  
Early Onset ◽  
Red Cell ◽  
Cell Distribution ◽  
Distribution Width ◽  
Term Neonates ◽  
Diagnosis And Prognosis

Objective: To assess the increased Red Cell Distribution Width (RDW) in diagnosis and prognosis of early-onset neonatal sepsis in term neonates. Methods: In a prospective, observational study, we enrolled term neonates ( 37 weeks of gestation) clinically suspected for Early-Onset Neonatal Sepsis (EONS) (within 7 days of birth). A cut-off of 18% and above was taken to consider RDW as abnormal or increased. The primary outcome was to assess the relation of increased RDW with in-hospital mortality. The secondary outcome was to determine the diagnostic yield of increased RDW in culture-proven sepsis. Results: In 166 neonates, 60% were males. Increased RDW was seen in 42.42% of neonates and 15.75% of neonates had positive blood culture. Compared to normal RDW, in-hospital mortality was significantly higher in neonates with increased mortality (27.14% vs. 10.52%, respectively; p=0.006). Also, abnormal RDW was seen in 46.15% of neonates with positive blood culture compared to 35.25% of neonates with negative blood culture (p<0.0001). Thus, elevated RDW had a sensitivity of 44.4% and specificity of 57.97% in the diagnosis of EONS. Conclusion: Increased RDW can be a diagnostic as well as a prognostic marker in neonates with EONS. Such observation indicates it may serve as a simple and easily available marker for EONS in resource-limited settings. However, these findings need to be confirmed in a larger sample. Doi: 10.28991/SciMedJ-2021-0303-7 Full Text: PDF

scholarly journals Serum Procalcitonin and C Reactive Protein in as early markers neonatal sepsis- A prospective study

2020 ◽  
Vol 8 (2) ◽  
pp. 73-77
Keyword(s):  
Blood Culture ◽  
Prospective Study ◽  
Gestational Age ◽  
Neonatal Sepsis ◽  
Statistical Significance ◽  
Developed Countries ◽  
Early Onset Sepsis ◽  
The Developed Countries ◽  
One Year ◽  
A Prospective Study

Neonatal sepsis is one of the commonest causes of morbidity and mortality in neonates in India compared to the developed countries. Aim: To evaluate the Procalcitonin level this is an early marker in the diagnosis of neonatal sepsis and to assess the suitability of this test in the diagnosis of early-onset sepsis. Method: The prospective study was conducted in the Neonatal Division of Department of Pediatrics, Prathima Institute of Medical Sciences over a period of one year. The blood samples from 100 babies meeting the inclusion and exclusion criteria constituted the material for study. Result: Among the n=100 cases n=39 were procalcitonin positive, compared with gestational age 10 (43.5%) cases were positive with a gestation of <37 weeks and 24 (31.2%) cases positive of cases >37 weeks and there was no statistical significance concerning gestational age the association of material characteristics with procalcitonin positive and CRP positive levels. Blood culture was positive in n=9 (9%) of babies with (90% CI, 5.3-14.9) and negative in n=91 (91%) of babies with (90% CI, 85.2-94.7). Conclusion: A positive blood culture is the only definitive and gold standard for confirming a case of sepsis. Since the culture and sensitivity test requires a minimum period of 48 hours which is a precious time in deciding on the treatment of sepsis in the newborn. Rapid diagnosis by using Procalcitonin and CRP gives a reasonable degree of accuracy in diagnosing neonatal sepsis and will also guide antibiotic therapy. Procalcitonin in comparison with CRP has better sensitivity and hence can detect most cases of neonatal sepsis and better negative predictive value.

P954 Predictors of positive blood culture in an emergency service. A prospective study

2007 ◽  
Vol 29 ◽  
pp. S250-S251
Author(s):  
B. Ceballos ◽  
G. Peralta ◽  
L. Ansorena ◽  
M. Rodriguez-Lera ◽  
M. Roiz ◽  
...  
Keyword(s):  
Blood Culture ◽  
Prospective Study ◽  
Positive Blood Culture ◽  
Emergency Service ◽  
A Prospective Study

scholarly journals Haematological Parameters in Neonatal Sepsis in a Tertiary Care Centre Study

2021 ◽  
Vol 8 (13) ◽  
pp. 751-754
Author(s):  
Pruthvi D ◽  
Pavan Ramarao Kulkarni ◽  
Uma Raghavendra Jamkhandi ◽  
Shivakumar Sanganagouda Inamdar
Keyword(s):  
Blood Culture ◽  
Absolute Neutrophil Count ◽  
Neutrophil Count ◽  
Neonatal Sepsis ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Neonatal Septicaemia ◽  
Sepsis Screen ◽  
Sensitivity Specificity ◽  
Culture Positive

BACKGROUND Neonatal septicaemia is a bacterial infection with positive blood culture in first four weeks of life. The early sepsis screen is vital as it detects earlier and benefits the clinician to treat the infection reducing neonatal mortality and morbidity. We wanted to evaluate various haematological screening parameters and C-reactive protein (CRP) in blood culture positive neonates in sepsis. METHODS This prospective study was conducted for a period of one year. Blood samples from hundred clinically suspected neonatal septicaemia cases were subjected to aerobic culture and sepsis screen tests like C-reactive protein, erythrocyte sedimentation ratio (ESR), total WBC count, absolute neutrophil count, immature / total neutrophil count (I / T) ratio and platelet count. The culture results were correlated with the sepsis screen tests. RESULTS Of the hundred cases studied, 18 % were blood culture positive and 66 % were males. Early onset septicaemia was more common, seen in 64 % of cases than late onset septicaemia (26 %) cases. Staphylococcus aureus was the commonest organism isolated in 38.46 % of cases followed by Klebsiella pneumoniae & E. Coli. Among the haematological parameters, the positivity was best with Creactive protein (94.44 %) followed by immature & mature neutrophil ratio (I / M) (94.44 %), I / T ratio (88.88 %) and the least with absolute neutrophil count (66.0 %). Any two or more parameters were positive in 94.44 % of the subjects. CONCLUSIONS Sepsis screen has good sensitivity, specificity and is a valuable aid for early diagnosis of neonatal septicaemia. Sepsis screen is simple, cost effective, less time consuming and easy to perform. As an individual test C-reactive protein has shown highest sensitivity, specificity and is a sensitive and responsive indicator of neonatal sepsis. KEYWORDS Blood Culture, Neonatal Septicaemia, Sepsis Screen

scholarly journals Gastric aspirate cytology as a screening tool for neonatal sepsis: a prospective study from a tertiary care centre

2018 ◽  
Vol 5 (4) ◽  
pp. 1662
Author(s):  
Ranjith Kumar ◽  
Bhaskar Reddy ◽  
Chapay Soren ◽  
Venkataramana Reddy ◽  
Raheemunisa .
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Neonatal Sepsis ◽  
Screening Tool ◽  
Tertiary Care ◽  
Neonatal Infection ◽  
Gastric Aspirate ◽  
Tertiary Care Centre ◽  
High Power Field ◽  
Culture Negative

Background: Neonatal sepsis is a clinical syndrome of bacteremia characterized by systemic signs and symptoms of infection in the first 28 days of life. It is responsible for 30-50% of the total neonatal deaths each year in developing countries. Gastric aspirate cytology has been used for neonatal infection. The presence of more than five polymorphs per high power field co-relate with neonatal infection. The objective of the present study was to evaluate the utility of gastric aspirate cytology as a screening tool for neonatal sepsis and to determine the polymorphonuclear leukocytes count in smear of gastric aspirate and correlating it with culture proven sepsis.Methods: This prospective observational study was conducted from February 2017 to January 2018 at level III Neonatal intensive care Unit of Sri Venkata Sai Medical College and Hospital, Mahabubnagar, Telangana. A total of 108 neonates with risk factor and / or clinical features of sepsis were included in the study.Results: Out of 108 neonates, 40 were blood culture positive   and 68 were culture negative. Gastric aspirate smear showed ≥5 polymorphs in 30 and <5 polymorphs in 10 neonates with positive blood culture. Among blood culture negative cases, 20 had ≥5 polymorphs and 48 had <5 polymorphs in gastric aspirate smear. Gastric aspirate culture was positive in 48 neonates and negative in 60 neonates. Of the 48 gastric aspirate positive neonates, 45 had ≥5 polymorphs and 3 had <5 polymorphs in gastric aspirate smear. Similarly, among 60 gastric aspirate culture negative neonates, 55 had ≥5 polymorphs and 3 had <5 polymorphs in gastric aspirate smear. This was statistically significant (P<0.000001). Of 48 neonates with positive gastric aspirate culture, 30 had positive blood culture and 18 had negative blood culture.Conclusions: Gastric aspirate cytology is a good screening tool for neonatal sepsis added to a detailed perinatal history and clinical examination but does not completely substitute the present day available screening parameters.

scholarly journals Prevalence and Severity of Thrombocytopenia in Blood Culture Proven Neonatal Sepsis: A Prospective Study

2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Ramesh Bhat Y. ◽  
Phalguna Kousika ◽  
Leslie Lewis ◽  
Jayashree Purkayastha
Keyword(s):  
Blood Culture ◽  
Prospective Study ◽  
Neonatal Sepsis ◽  
A Prospective Study

scholarly journals Prospective study to evaluate the risk factors associated with mortality in neonatal septicemia

2017 ◽  
Vol 4 (5) ◽  
pp. 1687
Author(s):  
Mandeep Singh Khurana ◽  
Supriya Malik ◽  
Gursharan Singh Narang ◽  
Ritish Saini
Keyword(s):  
Risk Factors ◽  
Birth Weight ◽  
Blood Culture ◽  
Low Birth Weight ◽  
Positive Blood Culture ◽  
Neonatal Sepsis ◽  
Clinical Syndrome ◽  
Preterm Baby ◽  
Neonatal Septicemia ◽  
Factors Associated

Background: Neonatal sepsis is a clinical syndrome of bacteremia characterized by systemic signs and symptoms of infection in the first month of life. Neonatal sepsis refers to infection occuring within the neonatal period i.e. first 28 days of life for a term baby and up to 4 weeks beyond the expected date of delivery in a preterm baby. Neonatal sepsis is one of the major cause of neonatal morbidity and mortality.Methods: The present study is a prospective observational study conducted in the neonatal intensive care unit (NICU) of Sri Guru Ram Das Institute of Medical Sciences and Research (SGRDIMSAR), Sri Amritsar over a period of one year from January 2015 to January 2016. Aim of the study is to know the incidence of neonatal sepsis, to study bacteriology of neonatal septicemia and to evaluate the risk factors associated with mortality in neonatal sepsis in our hospital.Results: During the study period (January 2015 to January 2016), 727 neonates were admitted in NICU of SGRDIMSAR, Sri Amritsar. Out of them, 109 neonates were diagnosed as having septicemia. Incidence of neonatal sepsis in our hospital in this study is 149.9/1000 neonatal admissions. 59 (54.1%) neonates had positive blood culture. Out of 109 septic neonates, 50 died and 59 survived. Mortality observed in the present study is 45.9%.Conclusions: It is concluded from present study that the main factors associated with neonatal mortality are low birth weight, prematurity, positive blood culture, neutropenia and prolonged prothrombin time. Some of the risk factors like low birth weight and prematurity are preventable by proper antenatal check ups and measures can be taken for safe and hygienic delivery. Babies with risk factors should be monitored closely for early detection of sepsis and the neonates with sepsis having abnormal laboratory parameters should be subjected to appropriate therapeutic intervention in order to decrease the mortality. 

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