GROWTH, FOOD CONSUMPTION, AND NITROGEN AND LIPID COMPOSITIONS OF THE COLORADO POTATO BEETLE, LEPTINOTARSA DECEMLINEATA, (COLEOPTERA: CHRYSOMELIDAE), AS A FUNCTION OF THE NITROGEN SUPPLY OF ITS HOST PLANT

1989 ◽  
Vol 24 (1) ◽  
pp. 62-69 ◽  
Author(s):  
Albert Zitzman ◽  
Michael L. May
Keyword(s):  
Lipid Composition ◽  
Leptinotarsa Decemlineata ◽  
Mass Gain ◽  
Dry Mass ◽  
Food Utilization ◽  
Mass Consumption ◽  
Potato Plants ◽  
N Supply ◽  
Potato Beetle ◽  
Rearing Conditions

Experiments were designed to investigate effects of nitrogen (N) fertilizer supplied to potato plants on growth and food utilization of larval Colorado potato beetles (CPB). Dry mass gain, dry mass consumption, and efficiency of conversion of ingested food (ECI = dry mass gain/dry mass consumption) were determined for the entire larval stage and nitrogen and lipid compositions for larvae just prior to pupation. Dry mass gain and N composition were measured in both laboratory and greenhouse reared larvae, dry mass consumption and ECI in laboratory reared larvae, and lipid composition in greenhouse reared larvae. Significant linear increases with N supply were found for dry mass gain of greenhouse reared larvae, but not laboratory reared larvae, and for N composition of laboratory reared larvae, but not greenhouse reared larvae. No significant effects were demonstrable for consumption and ECI of laboratory reared larvae, or lipid composition of greenhouse reared larvae. These results suggest that N supply can affect performance of CPB larvae but that the effects vary with small differences in rearing conditions.

scholarly journals Efficacy of the Applied Natural Enemies on the Survival of Colorado Potato Beetle Adults

Insects ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1030
Author(s):  
Vladimír Půža ◽  
Jiří Nermuť ◽  
Jana Konopická ◽  
Oxana Skoková Habuštová
Keyword(s):  
Emerging Adults ◽  
Natural Enemies ◽  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Entomopathogenic Fungus ◽  
Entomopathogenic Nematodes ◽  
Field Application ◽  
Fungal Treatment ◽  
Potato Plants ◽  
Potato Beetle

Colorado potato beetle Leptinotarsa decemlineata is among the most destructive pests of potatoes quickly developing resistance to traditional insecticides. In the present study, we tested the effect of various species and strains of entomopathogenic nematodes on CPB adults, and subsequently, the most effective nematodes were applied alone and in combination with entomopathogenic fungus B. bassiana in pots with potato plants and in the field and their effect on the number of emerging adults was evaluated. In the experimental infections, both the nematode invasion and pathogenicity were variable, and, in several strains, the mortality reached 100%. In pot experiments, soil application of nematodes S. carpocapsae 1343 and S. feltiae Jakub and fungus significantly decreased numbers of emerging CPB adults, while, after the application on leaves, only fungal treatment was effective. The field application of fungus B. bassiana significantly decreased the number of emerging CPB adults in comparison to control sites by ca. 30% while the effect of nematodes and the nematodes–fungus combination was not significant. In conclusion, we demonstrate the necessity of thorough bioassays to select the most effective nematode strains. Entomopathogenic nematodes have the potential to effectively decrease the emergence of CPB adults, but further research is needed to improve the effectiveness in the field.

scholarly journals Transcriptomics-Based Approach Identifies Spinosad-Associated Targets in the Colorado Potato Beetle, Leptinotarsa decemlineata

Insects ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 820
Author(s):  
Pierre Bastarache ◽  
Gabriel Wajnberg ◽  
Pascal Dumas ◽  
Simi Chacko ◽  
Jacynthe Lacroix ◽  
...  
Keyword(s):  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Insect Pest ◽  
Molecular Targets ◽  
Primary Method ◽  
Juvenile Hormone Esterase ◽  
Potato Plants ◽  
Potato Beetle ◽  
Potato Crops ◽  
Generation Sequencing

The Colorado potato beetle Leptinotarsa decemlineata is an insect pest that threatens potato crops globally. The primary method to control its damage on potato plants is the use of insecticides, including imidacloprid, chlorantraniliprole and spinosad. However, insecticide resistance has been frequently observed in Colorado potato beetles. The molecular targets and the basis of resistance to imidacloprid and chlorantraniliprole have both been previously quantified. This work was undertaken with the overarching goal of better characterizing the molecular changes associated with spinosad exposure in this insect pest. Next-generation sequencing was conducted to identify transcripts that were differentially expressed between Colorado potato beetles exposed to spinosad versus control insects. Results showed several transcripts that exhibit different expression levels between the two conditions, including ones coding for venom carboxylesterase-6, chitinase 10, juvenile hormone esterase and multidrug resistance-associated protein 4. In addition, several microRNAs, such as miR-12-3p and miR-750-3p, were also modulated in the investigated conditions. Overall, this work reveals a molecular footprint underlying spinosad response in Colorado potato beetles and provides novel leads that could be targeted as part of RNAi-based approaches to control this insect pest.

Season-Long Abundance of Generalist Predators in Transgenic versus Nontransgenic Potato Fields2

2000 ◽  
Vol 35 (4) ◽  
pp. 349-359 ◽  
Author(s):  
E. W. Riddick ◽  
G. Dively ◽  
P. Barbosa
Keyword(s):  
Relative Abundance ◽  
Leptinotarsa Decemlineata ◽  
Generalist Predators ◽  
Potato Plants ◽  
Naturally Occurring ◽  
Potato Beetle ◽  
Plant Foraging ◽  
Lady Beetles ◽  
Leptinotarsa Decemlineata Say ◽  
Transgenic Potatoes

We estimated the effect of deploying Cry3A-transgenic potatoes resistant to the Colorado potato beetle, Leptinotarsa decemlineata (Say), on the season-long relative abundance of naturally-occurring generalist predators. Low inputs of foliar insecticides were used in the transgenic fields to suppress nontarget pests and in the nontransgenic fields to prevent total defoliation of potato plants by L. decemlineata. Dominant plant-foraging heteropteran predators and lady beetles were sampled by sweeping foliage, whereas, ground-foraging carnivorous carabids, ants, and spiders were sampled by trapping in pitfalls. Orius insidiosus (Say) was significantly (P ≤ 0.05) more abundant in transgenic treatment fields than in nontransgenic fields in 1994, but not in 1995. None of the coccinellids (3 taxa) were affected by the treatments in either season. The carnivorous carabids (3 taxa) and ants were not affected by either treatment, but spiders were significantly more abundant in the transgenic treatment fields in 1995. We conclude that the deployment of pure stands of Cry3A-transgenic potatoes, with a minimum input of insecticides to suppress non-target pests, will have no deleterious effects on the populations of generalist predators in the potato ecosystem.

scholarly journals Glyphosate-based herbicide affects the composition of microbes associated with Colorado potato beetle (Leptinotarsa decemlineata)

2020 ◽  
Vol 367 (6) ◽  
Author(s):  
Carlos Gómez-Gallego ◽  
Miia J Rainio ◽  
M Carmen Collado ◽  
Anastasia Mantziari ◽  
Seppo Salminen ◽  
...  
Keyword(s):  
Leptinotarsa Decemlineata ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Microbial Composition ◽  
Untreated Soil ◽  
Potato Plants ◽  
Potato Beetle ◽  
Rrna Gene Sequencing ◽  
Two Phases

ABSTRACT Here, we examined whether glyphosate affects the microbiota of herbivores feeding on non-target plants. Colorado potato beetles (Leptinotarsa decemlineata) were reared on potato plants grown in pots containing untreated soil or soil treated with glyphosate-based herbicide (GBH). As per the manufacturer's safety recommendations, the GBH soil treatments were done 2 weeks prior to planting the potatoes. Later, 2-day-old larvae were introduced to the potato plants and then collected in two phases: fourth instar larvae and adults. The larvae's internal microbiota and the adults’ intestinal microbiota were examined by 16S rRNA gene sequencing. The beetles’ microbial composition was affected by the GBH treatment and the differences in microbial composition between the control and insects exposed to GBH were more pronounced in the adults. The GBH treatment increased the relative abundance of Agrobacterium in the larvae and the adults. This effect may be related to the tolerance of some Agrobacterium species to glyphosate or to glyphosate-mediated changes in potato plants. On the other hand, the relative abundances of Enterobacteriaceae, Rhodobacter, Rhizobium and Acidovorax in the adult beetles and Ochrobactrum in the larvae were reduced in GBH treatment. These results demonstrate that glyphosate can impact microbial communities associated with herbivores feeding on non-target crop plants.

scholarly journals Influence of High Temperature and Reduced Irradiance on Glycoalkaloid Levels in Potato Leaves

2000 ◽  
Vol 125 (5) ◽  
pp. 563-566 ◽  
Author(s):  
Abbas M. Lafta ◽  
James H. Lorenzen
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Temperature ◽  
Leptinotarsa Decemlineata ◽  
High Performance ◽  
High Irradiance ◽  
Effect Of Temperature ◽  
Potato Plants ◽  
Potato Beetle ◽  
Total Glycoalkaloids

Growth chamber and greenhouse experiments were conducted to investigate the effect of temperature and irradiance on foliar glycoalkaloids of three potato genotypes (Solanum tuberosum L.) that differ in glycoalkaloid content. Two genotypes (ND4382-17 and ND4382-19) produced the acetylated glycoalkaloids, leptine I and II, that contribute resistance to the Colorado potato beetle (CPB, Leptinotarsa decemlineata Say). The glycoalkaloids were separated and quantified by high performance liquid chromatography. Exposure of plants to high temperature (32/27 °C, 14-hour day/10-hour night) for 3 weeks under a 14-hour photoperiod with an irradiance of 475 μmol·m-2·s-1 significantly increased the levels of leptines I and II, solanine, and chaconine compared to that at low temperature (22/17 °C). Increases in foliar leptines and total glycoalkaloids at high temperature were 90% and 169%, respectively. Growing potato plants at low irradiance (75% reduction) for 2 or 4 weeks resulted in a significant reduction in the levels of leptine I and II (46%), solanine (43%), and chaconine (38%) compared to nonshaded plants. Transferring plants from high to low irradiance or from low to high irradiance for 2 weeks caused a decrease and an increase in glycoalkaloid concentration, respectively. Therefore, both temperature and irradiance influenced foliar levels of glycoalkaloids in potato plants without changing the leptines and solanine to chaconine ratios. Thus, irradiance and temperature influenced glycoalkaloid compounds that can effect resistance to CPB, especially leptine I and II.

Growth and development of Colorado potato beetle larvae, Leptinotarsa decemlineata, on potato plants expressing the oryzacystatin II proteinase inhibitor

2015 ◽  
Vol 24 (4) ◽  
pp. 729-740 ◽  
Author(s):  
Aleksandar Cingel ◽  
Jelena Savić ◽  
Branka Vinterhalter ◽  
Dragan Vinterhalter ◽  
Miroslav Kostić ◽  
...  
Keyword(s):  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Proteinase Inhibitor ◽  
Growth And Development ◽  
Potato Plants ◽  
Potato Beetle

INTERACTIONS AT THREE TROPHIC LEVELS: EDOVUM PUTTLERI GRISSELL (HYMENOPTERA: EULOPHIDAE), THE COLORADO POTATO BEETLE, AND INSECT-RESISTANT POTATOES

1989 ◽  
Vol 121 (10) ◽  
pp. 841-851 ◽  
Author(s):  
John R. Ruberson ◽  
Maurice J. Tauber ◽  
Catherine A. Tauber ◽  
Ward M. Tingey
Keyword(s):  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Life History Traits ◽  
Glandular Trichomes ◽  
Trophic Levels ◽  
The Other ◽  
Edovum Puttleri ◽  
Potato Plants ◽  
Potato Beetle ◽  
Insect Resistant

AbstractResistant potato plants influenced the parasitoid Edovum puttleri Grissell directly, as well as indirectly through eggs of the parasitoid’s host, the Colorado potato beetle, Leptinotarsa decemlineata (Say). In the field, E. puttleri parasitized more egg masses on plants with no glandular trichomes or with glandular trichomes bearing only enclosed droplets of exudate than on plants with two types of glandular trichomes (one with exposed droplets and the other bearing enclosed droplets). Trichomes with exposed droplets entrapped numerous parasitoids. Although rearing L. decemlineata on resistant plants influenced many of the beetle’s life-history traits, it did not affect the suitability of their eggs for development and survival of preimaginal E. puttleri. However, eggs from L. decemlineata that were reared on resistant potato plants reduced the longevity of the parasitoids and also reduced the number of hosts (eggs) killed by parasitoids.

scholarly journals AFLP-Based Genetic Linkage Map of the Colorado Potato Beetle Leptinotarsa decemlineata: Sex Chromosomes and a Pyrethroid-Resistance Candidate Gene

Genetics ◽  
2001 ◽  
Vol 158 (2) ◽  
pp. 695-700 ◽  
Author(s):  
David J Hawthorne
Keyword(s):  
Candidate Gene ◽  
Linkage Map ◽  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Sex Chromosome ◽  
Pyrethroid Resistance ◽  
Codominant Markers ◽  
Potato Beetle

Abstract A genetic linkage map was constructed from an intraspecific cross of the Colorado potato beetle, Leptinotarsa decemlineata. This is an initial step toward mapping the loci that underlie important phenotypes associated with insect adaptation to an agroecosystem. The map was made with 172 AFLP and 10 anonymous codominant markers segregating among 74 backcross (BC1) individuals. Markers were mapped to 18 linkage groups and a subset of the markers with a mean intermarker distance of 11.1 cM is presented. A pyrethroid-resistance candidate gene, LdVssc1, was placed onto the map as well. The sex chromosome was identified by exploiting the XO nature of sex determination in this species using patterns of variation at LdVssc1 and the codominant markers.

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