An Overview of the Application of Poly(lactic-co-glycolic) Acid (PLGA)-Based Scaffold for Drug Delivery in Cartilage Tissue Engineering

2021 ◽  
Author(s):  
Majid Pourentezari ◽  
Hengameh Dortaj ◽  
Batool Hashemibeni ◽  
Maryam Yadegari ◽  
Abbas Shahedi
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Glycolic Acid ◽  
Biomedical Application ◽  
Cartilage Tissue Engineering ◽  
Biological Properties ◽  
Cartilage Tissue ◽  
Plga Scaffold ◽  
Small Molecule Drugs ◽  
Synthetic Scaffold

Poly(lactic-co-glycolic) acid (PLGA) has attracted a considerable amount of interest for biomedical application due to its biocompatibility, tailored biodegradation rate (depending on the molecular weight and copolymer ratio), approval for clinical use in humans by the U.S. Food and Drug Administration (FDA), the potential to change surface properties to create better interaction with biological materials and being suitable for export to countries and cultures where planting products with animals is unusable. For commercial use and research, PLGA has been widely studied to control small molecule drugs, proteins, and other macromolecules. This study aims to review the studies that used PLGA scaffolding and its composites as a scaffold and drug delivery in cartilage tissue engineering. It is concluded from the results that the PLGA scaffold as a synthetic scaffold, when combined with natural scaffolds or hybrids, strengthens its biological properties and performs its function better.

Cartilage tissue engineering with controllable shape using a poly(lactic-co-glycolic acid)/collagen hybrid scaffold

2013 ◽  
Vol 28 (3) ◽  
pp. 247-257 ◽  
Author(s):  
Wenda Dai ◽  
Zhenjun Yao ◽  
Jian Dong ◽  
Naoki Kawazoe ◽  
Chi Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Glycolic Acid ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Hybrid Scaffold

scholarly journals Fabrication of Injectable Chitosan-Chondroitin Sulfate Hydrogel Embedding Kartogenin-Loaded Microspheres as an Ultrasound-Triggered Drug Delivery System for Cartilage Tissue Engineering

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1487
Author(s):  
Fu-Zhen Yuan ◽  
Hu-Fei Wang ◽  
Jian Guan ◽  
Jiang-Nan Fu ◽  
Meng Yang ◽  
...  
Keyword(s):  
Mechanical Property ◽  
Drug Delivery ◽  
Tissue Engineering ◽  
Chondroitin Sulfate ◽  
Drug Delivery System ◽  
Delivery System ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
In Vitro Degradation

Ultrasound-responsive microspheres (MPs) derived from natural polysaccharides and injectable hydrogels have been widely investigated as a biocompatible, biodegradable, and controllable drug delivery system and cell scaffolds for tissue engineering. In this study, kartogenin (KGN) loaded poly (lactide-co-glycolic acid) (PLGA) MPs (MPs@KGN) were fabricated by premix membrane emulsification (PME) method which were sonicated by an ultrasound transducer. Furthermore, carboxymethyl chitosan-oxidized chondroitin sulfate (CMC-OCS) hydrogel were prepared via the Schiff’ base reaction-embedded MPs to produce a CMC-OCS/MPs scaffold. In the current work, morphology, mechanical property, porosity determination, swelling property, in vitro degradation, KGN release from scaffolds, cytotoxicity, and cell bioactivity were investigated. The results showed that MPs presented an obvious collapse after ultrasound treatment. The embedded PLGA MPs could enhance the compressive elastic modulus of soft CMC-OCS hydrogel. The cumulative release KGN from MPs exhibited a slow rate which would display an appropriate collapse after ultrasound, allowing KGN to maintain a continuous concentration for at least 28 days. Moreover, the composite CMC-OCS@MPs scaffolds exhibited faster gelation, lower swelling ratio, and lower in vitro degradation. CCK-8 and LIVE/DEAD staining showed these scaffolds did not influence rabbit bone marrow mesenchymal stem cells (rBMMSCs) proliferation. Then these scaffolds were cultured with rBMMSCs for 2 weeks, and the immunofluorescent staining of collagen II (COL-2) showed that CMC-OCS hydrogel embedded with MPs@KGN (CMC-OCS@MPs@KGN) with ultrasound had the ability to increase the COL-2 synthesis. Overall, due to the improved mechanical property and the ability of sustained KGN release, this injectable hydrogel with ultrasound-responsive property is a promising system for cartilage tissue engineering.

scholarly journals Review of Synthetic and Hybrid Scaffolds in Cartilage Tissue Engineering

Membranes ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 348
Author(s):  
Monika Wasyłeczko ◽  
Wioleta Sikorska ◽  
Andrzej Chwojnowski
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Hybrid Materials ◽  
Cartilage Tissue Engineering ◽  
Biological Properties ◽  
General Information ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Natural Polymers ◽  
Cellular Environment

Cartilage tissue is under extensive investigation in tissue engineering and regenerative medicine studies because of its limited regenerative potential. Currently, many scaffolds are undergoing scientific and clinical research. A key for appropriate scaffolding is the assurance of a temporary cellular environment that allows the cells to function as in native tissue. These scaffolds should meet the relevant requirements, including appropriate architecture and physicochemical and biological properties. This is necessary for proper cell growth, which is associated with the adequate regeneration of cartilage. This paper presents a review of the development of scaffolds from synthetic polymers and hybrid materials employed for the engineering of cartilage tissue and regenerative medicine. Initially, general information on articular cartilage and an overview of the clinical strategies for the treatment of cartilage defects are presented. Then, the requirements for scaffolds in regenerative medicine, materials intended for membranes, and methods for obtaining them are briefly described. We also describe the hybrid materials that combine the advantages of both synthetic and natural polymers, which provide better properties for the scaffold. The last part of the article is focused on scaffolds in cartilage tissue engineering that have been confirmed by undergoing preclinical and clinical tests.

scholarly journals The Study on Biocompatibility of Porous nHA/PLGA Composite Scaffolds for Tissue Engineering with Rabbit ChondrocytesIn Vitro

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Lei Chen ◽  
Wei-Min Zhu ◽  
Zhi-Qiang Fei ◽  
Jie-Lin Chen ◽  
Jian-Yi Xiong ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Tissue Engineering ◽  
Flow Cytometry ◽  
Mtt Assay ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Control Group ◽  
Dependent Manner ◽  
Plga Scaffold ◽  
Significant Difference

Objective. To examine the biocompatibility of a novel nanohydroxyapatite/poly[lactic-co-glycolic acid] (nHA/PLGA) composite and evaluate its feasibility as a scaffold for cartilage tissue engineering.Methods. Chondrocytes of fetal rabbit were cultured with nHA/PLGA scaffoldin vitroand the cell viability was assessed by MTT assay first. Cells adhering to nHA/PLGA scaffold were then observed by inverted microscope and scanning electron microscope (SEM). The cell cycle profile was analyzed by flow cytometry.Results. The viability of the chondrocytes on the scaffold was not affected by nHA/PLGA comparing with the control group as it was shown by MTT assay. Cells on the surface and in the pores of the scaffold increased in a time-dependent manner. Results obtained from flow cytometry showed that there was no significant difference in cell cycle profiles between the coculture group and control (P>0.05).Conclusion. The porous nHA/PLGA composite scaffold is a biocompatible and good kind of scaffold for cartilage tissue engineering.

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