Determination of histamine in canned tuna fish available in Tehran market by ELISA method

2020 ◽  
Author(s):  
Naficeh Sadeghi ◽  
Masoomeh Behzad ◽  
Behrooz Jannat ◽  
Mohammad Reza Oveisi ◽  
Mannan Hajimahmoodi ◽  
...  
Keyword(s):  
Biogenic Amines ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Method ◽  
Average Amount ◽  
Histamine Concentration ◽  
Tuna Fish ◽  
Canned Tuna ◽  
Canned Fish ◽  
Standard Limit

Histamine is one of the well-known biogenic amines and responsible for causing allergic reactions. The presence of biogenic amines in the foodstuff is harmful, if it enters in a large amount to blood. In sea-food products, due to lack of proper storage at appropriate temperatures (freezing), histamine may be formed and will remain in the product, since it is already dry and heat resistance. Hazard of histamine consumption and average amount of canned fish consumed worldwide makes histamine measurement in canned fish very important. In this study 56 samples from 22 different brands were assessed and Enzyme-Linked Immunosorbent Assay (ELISA) was used by spectrophotometry for histamine detection. Our study showed that histamine levels in canned fish available in Tehran market, though it is high (5.75±5.98 mg/100 g tuna), but is not in a hazardous state (p<0.01). Our research showed that lowest and highest histamine concentration were 2.14 ±0.17 and 21.69±0.11 mg/100 g of fish respectively. It also indicates that medium does not affect the histamine content. There were no significant differences in the samples of fish and tuna fish for histamine. The amount of histamine in the tuna was below the standard limit (<50 mg histamine/100 g). Further studies should be carried out to investigate the presence of histamine in various fish and other sea-food.

Determination of ATP related compounds in fresh and canned tuna fish by HPLC

1997 ◽  
Vol 59 (3) ◽  
pp. 467-472 ◽  
Author(s):  
M.T. Veciana-Nogues ◽  
M. Izquierdo-Pulido ◽  
M.C. Vidal-Carou
Keyword(s):  
Tuna Fish ◽  
Canned Tuna ◽  
Related Compounds

scholarly journals Monitoring the Levels of Biogenic Amines in Canned Fish Products Marketed in Ghana

2020 ◽  
Vol 2020 ◽  
pp. 1-6 ◽  
Author(s):  
Alexander Weremfo ◽  
Meinster Kodjo Eduafo ◽  
Hakim Agyei Gyimah ◽  
Samuel Abassah-Oppong
Keyword(s):  
Biogenic Amines ◽  
Hplc Method ◽  
Ion Pair ◽  
Human Consumption ◽  
Fish Products ◽  
Canned Fish ◽  
Relative Standard ◽  
Excellent Selectivity ◽  
Detection And Quantification

An ion-pair HPLC method with postcolumn o-phthalaldehyde (OPA) derivatization and fluorescence detection was validated for quantitative determination of five biogenic amines (histamine, tyramine, cadaverine, putrescine, and agmatine) in canned fish products (mackerel, sardine, and tuna) marketed in Ghana. The validated method exhibited excellent selectivity and good linearity (R2 > 0.9990) for all the amines. The limits of detection and quantification for studied biogenic amines were in the range of 0.32–0.78 mg·kg−1 and 1.10–2.57 mg·kg−1, respectively. Also, a satisfactory recovery was obtained for all the amines (82.1–101.4%), and the relative standard deviations were lower than 9.3% under repeatability conditions for the studied amines. Subsequently, the method was applied to the analysis of biogenic amines in canned fish products to estimate the safety of Ghanaian consumers. The maximum levels of histamine, tyramine, cadaverine, putrescine, and agmatine detected in the analysed canned fish products were 64.05 mg·kg−1, 27.44 mg·kg−1, 27.23 mg·kg−1, 18.74 mg·kg−1, and 52.72 mg·kg−1, respectively. Thus, the levels of biogenic amines detected in the canned fish products were lower than the acceptable levels and, therefore, can be considered relatively safe for human consumption.

Validation Study of Histamine Test for the Determination of Histamine in Selected Fish Products

2019 ◽  
Vol 102 (1) ◽  
pp. 164-180 ◽  
Author(s):  
Kazuhiko Shimoji ◽  
Mikio Bakke ◽  
James M Hungerford ◽  
Christina A Mireles DeWitt ◽  
Sevim Köse
Keyword(s):  
Biogenic Amines ◽  
Validation Study ◽  
Optical Path Length ◽  
Cross Reactivity ◽  
Assay Method ◽  
Fish Sauce ◽  
Canned Tuna ◽  
Reactivity Test ◽  
Regulatory Bodies

Abstract Background: Since prescribed limits for histamine in fish have been set by various regulatory bodies around the world, the rapid, specific and easy determination of histamine is in high demand. Objective: The enzymatic histamine assay developed by Kikkoman Biochemifa Co. was validated. Methods: Fresh and frozen raw tuna, canned tuna in oil and water, and anchovy fish sauce were used for the validation study under the specific guidelines of the AOAC Research Institute Performance Tested MethodSM (PTM) program. Results: Good linearity (R2 &gt; 0.9997) for histamine standard solutions, recovery rates (90.3–125.2%), and repeatability precisions (RSDr &lt; 10%) were shown for all spiked tested matrixes. The claimed LOQ values, 20 and 10 mg/kg for solid fish and 160 and 80 mg/kg for fish sauce using a 1 and 2 cm optical path length spectrophotometer, respectively, were validated. The cross-reactivity test showed no positive interference of other biogenic amines, except for agmatine and putrescine. Moreover, no inhibition was confirmed among the 12 biogenic amines. Stability data supported the shelf life (42 months at 4°C), lot-to-lot consistency was demonstrated, and the method was shown to be robust. Independent laboratory testing using canned tuna in oil demonstrated that the recovery ranged from 93.5 to 124.7%, and the RSDr at all spike levels was &lt;20%. Conclusions: The simple and rapid enzymatic histamine assay method has been successfully validated. Highlights: This histamine quantitative assay kit was qualified for PTM certification No. 041802.

Occurrence of Histamine in Canned Fish Samples (Tuna, Sardine, Kilka, and Mackerel) from Markets in Tehran

2019 ◽  
Vol 83 (1) ◽  
pp. 136-141 ◽  
Author(s):  
LEILA PEIVASTEH-ROUDSARI ◽  
ANOSHEH RAHMANI ◽  
NABI SHARIATIFAR ◽  
BEHROUZ TAJDAR-ORANJ ◽  
MANSOOREH MAZAHERI ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Control Point ◽  
Uv Detector ◽  
Histamine Concentration ◽  
Critical Control Point ◽  
Canned Tuna ◽  
Canned Fish ◽  
Relative Standard ◽  
Significant Difference ◽  
Fish Samples

ABSTRACT Food poisoning is one of the most addressed health issues and has raised notable concerns. Histamine is the biogenic amine responsible for scombroid poisoning, which is due to the histidine decarboxylation by bacterial decarboxylases in various types of fish and fish products. The present investigation was conducted to measure the concentration of histamine in canned fish samples of tuna in oil (n = 18), tuna in oil with vegetables (n = 15), tuna in brine (n = 9), kilka in oil (n = 9), sardine in oil (n = 3), and mackerel in oil (n = 6) collected from markets in Tehran, Iran. Histamine concentrations were determined with a high-performance liquid chromatography device equipped with a UV detector. For method validation, the correlation coefficient (R2), recovery percentage, relative standard deviation for repeatability, limit of detection, and limit of quantification were 0.99, 82%, 1.3%, 1.5 mg/kg, and 5 mg/kg, respectively. Histamine was detected in 46.6% of the samples, and 18.3% of samples exceeded the histamine limit stipulated by the U.S. Food and Drug Administration (50 mg/kg). The overall mean histamine concentration was 17.36 ± 15.44 mg/kg, with a range of 0 to 88 mg/kg. A significant difference in histamine concentration was found between canned tuna in oil and canned tuna in brine (P &lt; 0.05). However, no significant difference in histamine concentration was found among samples of canned tuna in brine, canned sardine in oil, canned kilka in oil, and canned mackerel in oil. Because of the high histamine concentrations detected in some brands of Iranian canned tuna, precise control programs, hazard analysis critical control point systems, and good hygiene practices should be implemented. HIGHLIGHTS

scholarly journals Comparison of analysis and the nutritional value of fresh common carp, frozen and southern canned tuna

10.5219/1143 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 593-597 ◽  
Author(s):  
Ali Aberoumand ◽  
Afsaneh Fazeli
Keyword(s):  
Nutritional Value ◽  
Energy Content ◽  
Nutrient Composition ◽  
Fish Fillet ◽  
Tuna Fish ◽  
Fresh Fish ◽  
Canned Tuna ◽  
Canned Fish ◽  
Fresh Frozen ◽  
Frozen Fish

Freezing and canning are suitable methods to delay the spoilage of marine products and improve their physico-chemical and organoleptic properties. The fish were transported to the ice in proportion to 1 to 3 (w/w) inside the boxes, and then moved wastes. The purpose of this project is to analyse and to compare the nutritional value of fresh, frozen the fish and canned tuna fish. Nutrient composition and pH of the fresh fish fillet and moisture and ash contents and other nutrient composition were measured by the standard AOAC method. The results showed that the percentage of frozen fish protein was 17.41 and the highest moisture percentage for frozen fish with 72.23. The level of energy (kcal) of canned fish with 393.36 kcal was the highest level. The pH of the canned fish with 7.28 was the highest pH. The percentage of drip and WHC in frozen fillet found 6.7% and 6% respectively. From the obtained results, it can be concluded that despite the low amounts of protein and ash in canned fish, the fat and energy content was the highest. The protein content of the fish is frozen, and its pH indicates that it was better than fresh fish from point of quality.

Diarrhetic Shellfish Poisoning: Evaluation of Enzyme-Linked Immunosorbent Assay Methods for Determination of Dinophyslstoxin-2

1995 ◽  
Vol 78 (6) ◽  
pp. 1403-1407 ◽  
Author(s):  
Eoin P Carmody ◽  
Kevin J James ◽  
Seán S Kelly
Keyword(s):  
Immunosorbent Assay ◽  
Cross Reactivity ◽  
Regulatory Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Shellfish Poisoning ◽  
Diarrhetic Shellfish Poisoning ◽  
Elisa Method ◽  
Multiple Samples ◽  
Liquid Chromatographic

Abstract Dinophysistoxin-2 (DTX-2), an isomer of okadaic acid (OA), recently has been found in Irish waters. DTX-2 was the predominant toxin during prolonged infestations in cultivated mussels along the southwest coast of Ireland. Substantial variations in toxin levels may exist both horizontally and vertically in the water column. The need to take multiple samples and the ethical concern about the use of mammals for routine quality control of shellfish prompted examination of 2 commercially available enzyme-linked immunosorbent assay (ELISA) methods, designed to detect OA, for determination of both OA and DTX-2. One ELISA method (DSPCheck, Sceti Co. Ltd., (Tokyo, Japan) showed good cross-reactivity (40 ± 5%) with standard DTX-2. This study showed that both ELISA methods show good correlation with the liquid chromatographic analysis of 9-anthryldiazomethane derivatives when OA is the predominant toxin present. The sensitivity was also good for OA determination using both methods, which allowed toxin measurement at 10 ng/mL (0.5 ng/well). This level is equivalent to 0.03 μg/g mussel meat. Blank mussel samples spiked with DTX-2 standards gave a good linear correlation (r = 0.997) with this ELISA method when toxin levels were 0.03-0.3 μg/g mussel meat. This range is appropriate for regulatory control of diarrhetic shellfish poisoning.

Application of a novel electromembrane extraction and microextraction method followed by gas chromatography-mass spectrometry to determine biogenic amines in canned fish

2019 ◽  
Vol 11 (14) ◽  
pp. 1898-1907 ◽  
Author(s):  
Marzieh Kamankesh ◽  
Abdorreza Mohammadi ◽  
Afsaneh Mollahosseini ◽  
Shahram Seidi
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Biogenic Amines ◽  
Gas Chromatography Mass Spectrometry ◽  
Canned Fish ◽  
Fish Samples ◽  
Electromembrane Extraction ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

Electromembrane extraction (EME) and μ-dispersive liquid–liquid microextraction (μ-DLLME) have been applied in the simultaneous determination of biogenic amines (tyramine, histamine, putrescine and cadaverine) in canned fish samples.

Determination of ochratoxin A in serum and urine of pigs

2012 ◽  
Vol 5 (4) ◽  
pp. 351-356 ◽  
Author(s):  
N. Perši ◽  
J. Pleadin ◽  
A. Vulić ◽  
I. Kmetić ◽  
B. Šimić
Keyword(s):  
Ochratoxin A ◽  
Method Validation ◽  
Screening Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Method ◽  
Relative Standard ◽  
Experimental Group ◽  
Simple Screening ◽  
Serum And Urine

The objective of the study was to determine ochratoxin A (OTA) concentrations in serum and urine of pigs during 30-day OTA treatment. OTA was administered orally to the experimental group (n=5) at a dose of 0.78 mg per animal per day, whereas control animals (n=5) were left untreated. OTA concentrations were determined using a validated enzyme-linked immunosorbent assay (ELISA). Method validation resulted in mean recoveries of 93-101% for serum and 98-106% for urine, with acceptable mean inter- and intraday relative standard deviations (<8% for urine and <7% for serum). The ELISA method can be effectively used as a simple screening method to determine OTA exposure in pigs during fattening. The maximum mean OTA concentration in serum was recorded on day 22 (8.75±2.93 ng/ml) and in urine on day 20 (43.56±35.76 ng/ml), indicating significant differences in OTA concentrations between these two matrices.

Validation of a chemiluminescent assay for specific SARS-CoV-2 antibody

2020 ◽  
Vol 58 (8) ◽  
pp. 1357-1364 ◽  
Author(s):  
Marie Tré-Hardy ◽  
Alain Wilmet ◽  
Ingrid Beukinga ◽  
Jean-Michel Dogné ◽  
Jonathan Douxfils ◽  
...  
Keyword(s):  
Clinical Performance ◽  
Elisa Test ◽  
Diagnostic Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Elisa Method ◽  
Serological Tests ◽  
Economy Of Scale

AbstractObjectivesFaced with the COVID-19 pandemic and its impact on the availability and quality of both therapeutic and diagnostic methods, the Belgian authorities have decided to launch a procedure for additional evaluation of the performance of serological tests offered for sale on the national territory. This has been proposed with a double aim: (1) an in-depth verification of the analytical and clinical performances presented by the manufacturer and (2) an economy of scale in terms of centralized validation for all the laboratories using the tests subject to evaluation.MethodsA retrospective validation study was conducted including the serum of 125 patients in order to determine the analytical and clinical performances of the LIAISON®SARS-CoV-2 from DiaSorin® detecting anti-SARS-CoV-2 IgG and to compare its clinical performance with the enzyme-linked immunosorbent assay (ELISA) test from Euroimmun®, one of the first commercially available tests allowing the detection of anti-SARS-CoV-2 IgA and IgG.ResultsThe performances of the LIAISON®SARS-CoV-2 satisfied all the acceptance criteria and provided “real world” analytical and clinical performances very close to the ones reported by the manufacturer in its insert kit. Comparison between the LIAISON®SARS-CoV-2 and the ELISA method did not reveal any difference between the two techniques in terms of sensitivities and specificities regarding the determination of the IgG.ConclusionsThis study reports the validation of the LIAISON®SARS-CoV-2 allowing to detect IgG antibodies specifically directed against SARS-CoV-2. The analytical and clinical performances are excellent, and the automation of the test offers important rates, ideal for absorbing an extension of testing.

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