scholarly journals Antioxidant and Anticancer Activities of Anethum graveolens L., Citrus limon (L.) Osbeck and Zingiber officinale Roscoe Essential Oils

2022 ◽  
Author(s):  
Mahmoud Osanloo ◽  
Ali Ghanbariasad ◽  
Ali Taghinezhad
Keyword(s):  
Essential Oils ◽  
Antioxidant Activities ◽  
Zingiber Officinale ◽  
Gas Chromatography Mass Spectrometry ◽  
Breast Cancer Cell Lines ◽  
Citrus Limon ◽  
Anethum Graveolens ◽  
Anticancer Activities ◽  
Zingiber Officinale Roscoe ◽  
Mcf 7

Since synthetic chemotherapeutic drugs produce a certain degree of drug resistance and due to their common side effects, such as damage to hematopoietic cells and hair loss, it is necessary to use herbal medicine as a substrate to develop new anticancer drugs. The ingredients of three essential oils (EO) were identified using gas chromatography–mass spectrometry (GC-MS) analysis. Their anticancer activities have been investigated on four human breast cancer cell lines, including MCF-7, MDA-MB-175, MDA-MB-231, and MDA-MB-468. In addition, their antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The three plants were investigated for identifications of the ingredients of their EOs, and major ingredients were identified in each plant as alpha-phellandrene (26.75 %) in Anethum graveolens L., limonene (61.83 %) in Citrus limon (L.) Osbeck, and zingiberene (30.28 %) in Zingiber officinale Roscoe. Among the EOs, C. limon was significantly more effective than others; its half-maximal inhibitory concentration (IC50) on MCF-7 was obtained at 201 µg.mL-1. Furthermore, Z. officinale EO showed a higher antioxidant activities in comparison to the two other EOs. Considering the antioxidant and anticancer effects of the EOs, they could be further investigated as a possible complementary medicine in cancer.

scholarly journals Surveying the chemical composition and antibacterial activity of essential oils from selected medicinal plants against human pathogens

2020 ◽  
Author(s):  
Mahmoud Osanloo ◽  
Ghazal Ghaznavi ◽  
Abbas Abdollahi
Keyword(s):  
Essential Oils ◽  
Biological Activities ◽  
Antibacterial Properties ◽  
Zingiber Officinale ◽  
New Drugs ◽  
Citrus Limon ◽  
Human Pathogens ◽  
Anethum Graveolens ◽  
Cinnamomum Zeylanicum ◽  
Artemisia Dracunculus

Background and Objectives: Essential oils (EOs) with different biological activities, such as antibacterial properties, are a valuable resource for developing new drugs. Materials and Methods: Ingredients of six medicinally important EOs, including Artemisia dracunculus, Anethum graveolens, Citrus limon, Citrus sinensis, Cinnamomum zeylanicum and Zingiber officinale, were identified using GC-MS analysis. Moreover, their five major compounds were also listed. Furthermore, the half-maximal inhibitory concentration (IC50) against four important human bacteria was also investigated using the 96-well plate microdilution. Results: C. sinensis EO with IC50 of 1.0 and 4.7 mg.mL-1 have the most effect on the growth of S. aureus and P. aeruginosa. Moreover, EOs of Cinnamomum zeylanicum (IC50: 1.0 mg. mL-1) and Artemisia dracunculus (IC50: 1.3 mg.mL-1) significantly showed better inhibitory effect on E. coli and K. pneumoniae. Conclusion: These EOs could be used for developing inexpensive, potent, and green antibacterial agents.

scholarly journals BIOACTIVITIES AND CHEMOPROFILING COMPARISONS OF CHENOPODIUM AMBROSIOIDES L. AND CHENOPODIUM BOTRYS L. GROWING IN KASHMIR, INDIA

2019 ◽  
Vol 12 (1) ◽  
pp. 124 ◽  
Author(s):  
Shameem A Shameem ◽  
Khaliquz Z Khan ◽  
Ajaz A Waza ◽  
Abdul Haseeb Shah ◽  
Hafsa Qadri ◽  
...  
Keyword(s):  
Essential Oils ◽  
Cell Line ◽  
Chemical Constituents ◽  
Pathogenic Fungi ◽  
Gas Chromatography Mass Spectrometry ◽  
Chenopodium Ambrosioides ◽  
Anticancer Activities ◽  
Antifungal Activities ◽  
Microtiter Plates ◽  
Mcf 7

Objectives: The objectives of the study were the gas chromatography-mass spectrometry (GC-MS) identification and comparison of the chemical constituents, evaluation of the antifungal and anticancer activities of two species of genus Chenopodium, for example, Chenopodium ambrosioides (Ca) and Chenopodium botrys (Cb) growing in Kashmir, Himalayan region.Methods: The hydrodistilled essential oil of Ca and Cb was subjected to GC-MS analysis and antifungal activity for minimum inhibitory concentration (MIC) determination against different human pathogenic fungal strains using broth microdilution assay in 96-well microtiter plates as per the protocol of the clinical and laboratory standards institute (2008 M27-A3). The anti-proliferative ability of the essential oils was also evaluated against the two cell lines MCF-7 (human mammary carcinoma cells) and A549 (Human lung adenocarcinoma epithelial cells).Results: A total of 34 compounds identified in Ca with α-terpinene (37.17%), isoascaridole (20.48%), and ascaridole (14.83%) as the key compounds. The key compounds of Cb were shyobunol (18.91%), and hedycaryol (9.51%), germacrene-D-4-ol (8.57%), with 65 identified compounds. Both the species were found to have comparable antifungal activities against human pathogenic fungi with MIC80 values in the range of 0.031 mg/ml– 0.256 mg/ml for Ca and 0.031 mg/ml–0.126 mg/ml in case of Cb. Maximum anti-proliferative activity was observed at 125 μg/ml concentration in A549 cell line, while as the oils inhibited the growth of MCF-7 cell line at a lower concentration of 31.25 μg/ml.Conclusion: The essential oils of Ca and Cb were found to have potent anticancer and antifungal activities and can have potential use as a natural fungicide.

scholarly journals BIOACTIVITIES AND CHEMOPROFILING COMPARISONS OF CHENOPODIUM AMBROSIOIDES L. AND CHENOPODIUM BOTRYS L. GROWING IN KASHMIR, INDIA

2019 ◽  
Vol 12 (1) ◽  
pp. 124
Author(s):  
Shameem A Shameem ◽  
Khaliquz Z Khan ◽  
Ajaz A Waza ◽  
Abdul Haseeb Shah ◽  
Hafsa Qadri ◽  
...  
Keyword(s):  
Essential Oils ◽  
Cell Line ◽  
Chemical Constituents ◽  
Pathogenic Fungi ◽  
Gas Chromatography Mass Spectrometry ◽  
Chenopodium Ambrosioides ◽  
Anticancer Activities ◽  
Antifungal Activities ◽  
Microtiter Plates ◽  
Mcf 7

Objectives: The objectives of the study were the gas chromatography-mass spectrometry (GC-MS) identification and comparison of the chemical constituents, evaluation of the antifungal and anticancer activities of two species of genus Chenopodium, for example, Chenopodium ambrosioides (Ca) and Chenopodium botrys (Cb) growing in Kashmir, Himalayan region.Methods: The hydrodistilled essential oil of Ca and Cb was subjected to GC-MS analysis and antifungal activity for minimum inhibitory concentration (MIC) determination against different human pathogenic fungal strains using broth microdilution assay in 96-well microtiter plates as per the protocol of the clinical and laboratory standards institute (2008 M27-A3). The anti-proliferative ability of the essential oils was also evaluated against the two cell lines MCF-7 (human mammary carcinoma cells) and A549 (Human lung adenocarcinoma epithelial cells).Results: A total of 34 compounds identified in Ca with α-terpinene (37.17%), isoascaridole (20.48%), and ascaridole (14.83%) as the key compounds. The key compounds of Cb were shyobunol (18.91%), and hedycaryol (9.51%), germacrene-D-4-ol (8.57%), with 65 identified compounds. Both the species were found to have comparable antifungal activities against human pathogenic fungi with MIC80 values in the range of 0.031 mg/ml– 0.256 mg/ml for Ca and 0.031 mg/ml–0.126 mg/ml in case of Cb. Maximum anti-proliferative activity was observed at 125 μg/ml concentration in A549 cell line, while as the oils inhibited the growth of MCF-7 cell line at a lower concentration of 31.25 μg/ml.Conclusion: The essential oils of Ca and Cb were found to have potent anticancer and antifungal activities and can have potential use as a natural fungicide.

Composition of essential oil and antioxidant activity of Khat (Catha edulis Forsk), Ethiopia

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Bahir Dar ◽  
Dpph Radical ◽  
Catha Edulis

In this study, we determined the chemical composition and antioxidant activities of the essential oils from two different varieties of khat (Catha edulis Forsk) cultivated in Ethiopia. The essential oils were extracted by hydrodistillation using the Clevenger type apparatus, identifications of compounds were made by gas chromatography and gas chromatography-mass spectrometry (GC-MS). Seventy seven different compounds were identified from essential oils of the two different khat cultivars. The essential oils in the samples from Bahir Dar and Wendo were composed of 50 and 34 compounds, respectively. The major compound identified in khat essentials oils include: limonene, 1-phenyl-1,2-propanedione, 1-hydroxy,1-phenyl-2-propanone, camphor, (sulfurous acid)-2-propylundecyl ester, hexadecane, O-mentha-1(7), 8-dien-3-ol, heptadecane, 10-methylnonadecane, (phthalic acid)-isobutyl octadecyl ester, and tritetracontane. The antioxidant and free radical scavenging activity of the oils were assessed by means of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay. The scavenging activities of the oils were 23.5-23.6 μg AAE/kg of fresh khat sample.

scholarly journals Ducrosia ismaelis Asch. essential oil: chemical composition profile and anticancer, antimicrobial and antioxidant potential assessment

2020 ◽  
Vol 18 (1) ◽  
pp. 175-184 ◽  
Author(s):  
Ramzi A. Mothana ◽  
Fahd A. Nasr ◽  
Jamal M. Khaled ◽  
Omar M. Noman ◽  
Nael Abutaha ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Moderate Activity ◽  
Antioxidant Agent ◽  
Total Oil ◽  
Β Carotene ◽  
Mcf 7

AbstractThe essential oil of Ducrosia ismaelis Asch. (Apiaceae) that grows wild in Saudi Arabia was investigated utilizing gas chromatography (GC), and gas chromatography-mass spectrometry. Fifty constituents were characterized, representing 96.1% of the total oil. The D. ismaelis essential oil (DIEO) was distinguished by a high composition of oxygenated monoterpenes (51.6%). Decanal (40.6%), α-pinene (15.1%) and dodecanal (13.7%) were the fundamental components. Additionally, DIEO was evaluated for its cytotoxic, antibacterial, antifungal and antioxidant activities. DIEO revealed a great cytotoxic effectiveness against the tested cancer cell lines with IC50 values between 66.2 and 137.3 μg/mL particularly against MCF-7 cancer cells. Furthermore, the induction of apoptosis against MCF-7 cells has been asserted using staining assay (annexin VFITC and/or propidium iodide (PI) dyes) and flow cytometry technique. The DIEO possessed a strong antimicrobial activity against Gram-positive bacterial and fungal strains with MIC-values between 0.07 and 0.31 mg/ml. The values of MBC or MFC were almost once higher than those of MIC’s. Moreover, the β-carotene-bleaching and DPPH free radical-scavenging tests showed that DIEO had a moderate activity (68%) as an antioxidant agent in decolouring of the β-carotene at 1.0 mg/mL and a moderate radical scavenging for DPPH (66 and 72%) at 0.50 and 1.0 mg/mL.

scholarly journals Levantamento etnobotânico na Comunidade Rural de Santa Marta, Município de Corrente, Estado do Piauí, Nordeste do Brasil

2020 ◽  
Vol 7 (17) ◽  
pp. 1483-1498
Author(s):  
Cecília de Souza Carvalho ◽  
Patrine Nunes Gomes ◽  
Luzirrany Soares Lopes ◽  
Miria Cassia Oliveira Aragão ◽  
Lizandro Pereira de Abreu ◽  
...  
Keyword(s):  
Zingiber Officinale ◽  
Rosmarinus Officinalis ◽  
Citrus Limon ◽  
Mata Atlântica ◽  
Pimpinella Anisum ◽  
Lippia Alba ◽  
Zingiber Officinale Roscoe ◽  
Mata Atlantica ◽  
Rosmarinus Officinalis L ◽  
Mentha Crispa

O Brasil é um país bastante rico em biodiversidade e com isso as comunidades tradicionais e agricultores fazem o cultivo dessa ampla diversidade de espécies e de variedades vegetais. Este fator agrega grande relevância aos estudos etnobotânicos, onde busca-se resgatar o conhecimento sobre as plantas nas comunidades rurais, visando manter a interrelação entre a utilização de plantas medicinais e o conhecimento das pessoas. O presente estudo teve como objetivo verificar o perfil socioeconômico e a percepção das pessoas sobre o conhecimento botânico do uso e apropriação das plantas medicinais utilizadas na comunidade rural de Santa Marta, Município de Corrente, Estado do Piauí. A pesquisa foi realizada no período de julho de 2018 a março de 2019, na Comunidade Rural de Santa Marta, sendo ela destinada às pessoas com idade igual ou superior a 40 anos, em virtude de possuírem mais conhecimento sobre o uso de plantas medicinais. Para isso, foi realizada uma caminhada transversal com alguns moradores e a aplicação de um questionário semiestruturado de caráter quali-quantitavo. Foi registrado um total de 90 espécies de plantas medicinais utilizadas pelos moradores da Comunidade Rural de Santa Marta. Após identificar as espécies juntamente com os moradores e ainda com a realização de levantamentos bibliográficos, verificou-se que muitas destas espécies são típicas do Cerrado, mas existem a ocorrência de algumas predominantes da Caatinga e Mata Atlântica. As espécies mais usadas pelos entrevistados são a erva-doce Pimpinella anisum L. e o hortelã Mentha crispa L., mencionadas por cerca de 36,36% dos entrevistados, a melancia-da-praia Solanum agrarium Sendtn. (27,27%), a erva-cidreira Lippia alba Mill. N.E.Br. (18,18%), seguido do alecrim Rosmarinus officinalis L., do gengibre Zingiber officinale Roscoe, da imburana Amburana cearensis A.C. Smith e do limão Citrus limon L. Burm. (13,64%) e do pequi Caryocar coriaceum Wittm (4,54%). Dentre as espécies identificadas muitas delas são utilizadas para o tratamento de enfermidades que acometem a vida das pessoas como, diarreia, hipertensão, infecção no sistema urinário, colesterol, entre outros. Quanto à parte vegetal utilizada nas preparações dos remédios caseiros, observou-se uma frequência maior de uso das folhas, seguido de caule e fruta, raízes, flores e óleo. Por meio das informações obtidas foi possível realizar o levantamento etnobotânico das plantas medicinais utilizadas na Comunidade Rural de Santa Marta e, além disso, do uso e do conhecimento que as pessoas possuem. Ao construir essa relação entre comunidade e o conhecimento sobre uso das plantas medicinais, torna-se de grande valia para a vida das pessoas, propiciando a contribuição para a conservação da cultura regional, contribuindo assim na sua valorização.

scholarly journals Chemical Compositions and Antimicrobial and Antioxidant Activities of the Essential Oils from Magnolia grandiflora, Chrysactinia mexicana, and Schinus molle Found in Northeast Mexico

2013 ◽  
Vol 8 (1) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Laura Guerra-Boone ◽  
Rocío Álvarez-Román ◽  
Ricardo Salazar-Aranda ◽  
Anabel Torres-Cirio ◽  
Verónica Mayela Rivas-Galindo ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Principal Component ◽  
Gas Chromatography Mass Spectrometry ◽  
Bornyl Acetate ◽  
Chemical Compositions ◽  
Magnolia Grandiflora ◽  
Schinus Molle ◽  
Main Component

The essential oils from Magnolia grandiflora and Chrysactinia mexicana leaves, and from Schinus molle leaves and fruit, were characterized by gas chromatography/flame-ionization detection and gas chromatography/mass spectrometry. Twenty-eight compounds from M. grandiflora leaves were identified (representing 93.6% of the total area of the gas chromatogram), with the major component being bornyl acetate (20.9%). Colorless and yellow oils were obtained from the C. mexicana leaves with 18 (86.7%) and 11 (100%) compounds identified, respectively. In both fractions, the principal component was sylvestrene (36.8% and 41.1%, respectively). The essential oils of S. molle leaves and fruit were each separated into colorless and yellow fractions, in which 14 (98.2) and 20 (99.8%) compounds were identified. The main component was α-phellandrene in all fractions (between 32.8% and 45.0%). The M. grandiflora oil displayed antifungal activity against five dermatophyte strains. The oils from S. molle and M. grandiflora leaves had antimicrobial activity against Staphylococcus aureus and Streptococcus pyogenes, which cause skin infections that potentially may lead to sepsis. However, the antioxidant activities of all oils were small (half maximal effective concentration values >250 μg/mL).

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