scholarly journals BIOACTIVITIES AND CHEMOPROFILING COMPARISONS OF CHENOPODIUM AMBROSIOIDES L. AND CHENOPODIUM BOTRYS L. GROWING IN KASHMIR, INDIA

2019 ◽  
Vol 12 (1) ◽  
pp. 124
Author(s):  
Shameem A Shameem ◽  
Khaliquz Z Khan ◽  
Ajaz A Waza ◽  
Abdul Haseeb Shah ◽  
Hafsa Qadri ◽  
...  
Keyword(s):  
Essential Oils ◽  
Cell Line ◽  
Chemical Constituents ◽  
Pathogenic Fungi ◽  
Gas Chromatography Mass Spectrometry ◽  
Chenopodium Ambrosioides ◽  
Anticancer Activities ◽  
Antifungal Activities ◽  
Microtiter Plates ◽  
Mcf 7

Objectives: The objectives of the study were the gas chromatography-mass spectrometry (GC-MS) identification and comparison of the chemical constituents, evaluation of the antifungal and anticancer activities of two species of genus Chenopodium, for example, Chenopodium ambrosioides (Ca) and Chenopodium botrys (Cb) growing in Kashmir, Himalayan region.Methods: The hydrodistilled essential oil of Ca and Cb was subjected to GC-MS analysis and antifungal activity for minimum inhibitory concentration (MIC) determination against different human pathogenic fungal strains using broth microdilution assay in 96-well microtiter plates as per the protocol of the clinical and laboratory standards institute (2008 M27-A3). The anti-proliferative ability of the essential oils was also evaluated against the two cell lines MCF-7 (human mammary carcinoma cells) and A549 (Human lung adenocarcinoma epithelial cells).Results: A total of 34 compounds identified in Ca with α-terpinene (37.17%), isoascaridole (20.48%), and ascaridole (14.83%) as the key compounds. The key compounds of Cb were shyobunol (18.91%), and hedycaryol (9.51%), germacrene-D-4-ol (8.57%), with 65 identified compounds. Both the species were found to have comparable antifungal activities against human pathogenic fungi with MIC80 values in the range of 0.031 mg/ml– 0.256 mg/ml for Ca and 0.031 mg/ml–0.126 mg/ml in case of Cb. Maximum anti-proliferative activity was observed at 125 μg/ml concentration in A549 cell line, while as the oils inhibited the growth of MCF-7 cell line at a lower concentration of 31.25 μg/ml.Conclusion: The essential oils of Ca and Cb were found to have potent anticancer and antifungal activities and can have potential use as a natural fungicide.

scholarly journals BIOACTIVITIES AND CHEMOPROFILING COMPARISONS OF CHENOPODIUM AMBROSIOIDES L. AND CHENOPODIUM BOTRYS L. GROWING IN KASHMIR, INDIA

2019 ◽  
Vol 12 (1) ◽  
pp. 124 ◽  
Author(s):  
Shameem A Shameem ◽  
Khaliquz Z Khan ◽  
Ajaz A Waza ◽  
Abdul Haseeb Shah ◽  
Hafsa Qadri ◽  
...  
Keyword(s):  
Essential Oils ◽  
Cell Line ◽  
Chemical Constituents ◽  
Pathogenic Fungi ◽  
Gas Chromatography Mass Spectrometry ◽  
Chenopodium Ambrosioides ◽  
Anticancer Activities ◽  
Antifungal Activities ◽  
Microtiter Plates ◽  
Mcf 7

Objectives: The objectives of the study were the gas chromatography-mass spectrometry (GC-MS) identification and comparison of the chemical constituents, evaluation of the antifungal and anticancer activities of two species of genus Chenopodium, for example, Chenopodium ambrosioides (Ca) and Chenopodium botrys (Cb) growing in Kashmir, Himalayan region.Methods: The hydrodistilled essential oil of Ca and Cb was subjected to GC-MS analysis and antifungal activity for minimum inhibitory concentration (MIC) determination against different human pathogenic fungal strains using broth microdilution assay in 96-well microtiter plates as per the protocol of the clinical and laboratory standards institute (2008 M27-A3). The anti-proliferative ability of the essential oils was also evaluated against the two cell lines MCF-7 (human mammary carcinoma cells) and A549 (Human lung adenocarcinoma epithelial cells).Results: A total of 34 compounds identified in Ca with α-terpinene (37.17%), isoascaridole (20.48%), and ascaridole (14.83%) as the key compounds. The key compounds of Cb were shyobunol (18.91%), and hedycaryol (9.51%), germacrene-D-4-ol (8.57%), with 65 identified compounds. Both the species were found to have comparable antifungal activities against human pathogenic fungi with MIC80 values in the range of 0.031 mg/ml– 0.256 mg/ml for Ca and 0.031 mg/ml–0.126 mg/ml in case of Cb. Maximum anti-proliferative activity was observed at 125 μg/ml concentration in A549 cell line, while as the oils inhibited the growth of MCF-7 cell line at a lower concentration of 31.25 μg/ml.Conclusion: The essential oils of Ca and Cb were found to have potent anticancer and antifungal activities and can have potential use as a natural fungicide.

scholarly journals Antioxidant and Anticancer Activities of Anethum graveolens L., Citrus limon (L.) Osbeck and Zingiber officinale Roscoe Essential Oils

2022 ◽  
Author(s):  
Mahmoud Osanloo ◽  
Ali Ghanbariasad ◽  
Ali Taghinezhad
Keyword(s):  
Essential Oils ◽  
Antioxidant Activities ◽  
Zingiber Officinale ◽  
Gas Chromatography Mass Spectrometry ◽  
Breast Cancer Cell Lines ◽  
Citrus Limon ◽  
Anethum Graveolens ◽  
Anticancer Activities ◽  
Zingiber Officinale Roscoe ◽  
Mcf 7

Since synthetic chemotherapeutic drugs produce a certain degree of drug resistance and due to their common side effects, such as damage to hematopoietic cells and hair loss, it is necessary to use herbal medicine as a substrate to develop new anticancer drugs. The ingredients of three essential oils (EO) were identified using gas chromatography–mass spectrometry (GC-MS) analysis. Their anticancer activities have been investigated on four human breast cancer cell lines, including MCF-7, MDA-MB-175, MDA-MB-231, and MDA-MB-468. In addition, their antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The three plants were investigated for identifications of the ingredients of their EOs, and major ingredients were identified in each plant as alpha-phellandrene (26.75 %) in Anethum graveolens L., limonene (61.83 %) in Citrus limon (L.) Osbeck, and zingiberene (30.28 %) in Zingiber officinale Roscoe. Among the EOs, C. limon was significantly more effective than others; its half-maximal inhibitory concentration (IC50) on MCF-7 was obtained at 201 µg.mL-1. Furthermore, Z. officinale EO showed a higher antioxidant activities in comparison to the two other EOs. Considering the antioxidant and anticancer effects of the EOs, they could be further investigated as a possible complementary medicine in cancer.

scholarly journals Evaluation of Anticancer Activities of Clerodendrum formicarum Gurke (Lamiaceae) and Syzygium Cordatum Hochst ex Krauss (Myrtaceae) Harvested in the City of Lubumbashi and its Surroundings in the Democratic Republic Of The Congo

2020 ◽  
pp. 1-5
Author(s):  
Topwe Milongwe Mwene-Mbeja ◽  
Claude Spino ◽  
Donald Poirier ◽  
Emery Kalonda Mutombo ◽  
Jenny Roy ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Chemical Composition ◽  
Essential Oils ◽  
Democratic Republic ◽  
Chemical Constituents ◽  
Anticancer Activities ◽  
Unknown Compound ◽  
Republic Of The Congo ◽  
The City ◽  
Mcf 7

The main objective of this study was to extract the essential oils from Clerodendrum formicarum and Syzygium cordatum in order to determine the anticancer activity and establish the chemical constituents of that kind of essential oils. Hence, we have used hydrodistillation method to obtain the desired extracts or samples of essential oils. Indeed, the assessment of the anticancer activities showed that, at 100 µg/mL, the essential oils from S. cordatum inhibited the proliferation (98.2%) of breast cancer (MCF-7) cells. In the same context, the essential oils from C. formicarum also inhibited the proliferation (98.9%) of breast cancer (MCF-7) cells. We have additionally determined the chemical composition of the essential oils by GC-MS and identified 17 compounds in C. formicarum including caryophyllene (18.6%), (E)-3,7-dimethylocta1,3,6-triene (7.6%), β-Myrcene (6.2%), and [1S-(1α, 7α, 8α)]-1,8a-dimethyl-7-(1-methylethenyl)- 1,2,3,5,6,7,8,8a-octahydronaphthalene (5.6%) as major compounds. We have observed an unknown compound (25%) in the essential oils from C. formicarum (H-12) The chemical analysis showed 28 compounds in S. cordatum (HE-9) essential oils such as caryophyllene (7.2%) and cedrene (6.7%) as major compounds

Synthesis of a Series of Novel 2-Amino-5-Substituted 1,3,4-oxadiazole and 1,3,4-thiadiazole Derivatives as Potential Anticancer, Antifungal and Antibacterial Agents

2021 ◽  
Vol 17 ◽  
Author(s):  
Em Canh Pham ◽  
Tuyen Ngoc Truong ◽  
Nguyen Hanh Dong ◽  
Duy Duc Vo ◽  
Tuoi Thi Hong Do
Keyword(s):  
Cell Line ◽  
Biological Activities ◽  
Chemical Properties ◽  
Diffusion Method ◽  
Anticancer Activities ◽  
Molecular Docking Study ◽  
Antifungal Activities ◽  
Antibacterial And Antifungal Activities ◽  
Thiadiazole Derivatives ◽  
Antibacterial And Antifungal

Background: Many compounds containing a five-membered heterocyclic ring display exceptional chemical properties and versatile biological activities. Objective: The objective of the present study was the desire to prepare the 5-substituted 2-amino-1,3,4-oxadiazole and 2-amino-1,3,4-thiadiazole derivatives and evaluate their potential anticancer, antibacterial and antifungal activities. Methods: Twenty-seven derivatives were synthesized by iodine-mediated cyclization of semicarbazones or thiosemicarbazones obtained from condensation of semicarbazide or thiosemicarbazide and aldehydes. The structures were confirmed by 1H-NMR, 13C-NMR and MS spectra. The antibacterial and antifungal activities were evaluated by diffusion method and the anticancer activities were evaluated by MTT assay. Results: Twenty-seven derivatives have been synthesized in moderate to good yields. A number of derivatives exhibited potential antibacterial, antifungal and anticancer activities. Conclusion: Compounds (1b, 1e and 1g) showed antibacterial activity against Streptococcus faecalis, MSSA and MRSA with MIC ranging between 4 to 64 µg/mL. Compound (2g) showed antifungal activity against Candida albicans (8 µg/mL) and Aspergillus niger (64 µg/mL). Compound (1o) exhibited high cytotoxic activity against HepG2 cell line (IC50 value 8.6 µM), which is comparable to the activity of paclitaxel, and is non-toxic on LLC-PK1 normal cell line. The structure activity relationship and molecular docking study of the synthesized compounds are also reported.

scholarly journals Antiproliferative activity of essential oils from three plants of the Brazilian Cerrado: Campomanesia adamantium (Myrtaceae), Protium ovatum (Burseraceae) and Cardiopetalum calophyllum (Annonaceae)

2020 ◽  
Vol 80 (2) ◽  
pp. 290-294 ◽  
Author(s):  
C. C. F. Alves ◽  
J. D. Oliveira ◽  
E. B. B. Estevam ◽  
M. N. Xavier ◽  
H. D. Nicolella ◽  
...  
Keyword(s):  
Essential Oils ◽  
Cell Line ◽  
Tumor Cells ◽  
Antiproliferative Activity ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Human Tumor ◽  
Lung Fibroblasts ◽  
Breast Adenocarcinoma ◽  
Brazilian Cerrado

Abstract Essential oils, which may be extracted from several parts of plants, have different biological activities. The Brazilian Cerrado has a large variety of plants that yield essential oils, even though many have not been studied yet. Taking into account the biodiversity of this biome, this study aimed at evaluating the antiproliferative activity of essential oils extracted from three species of plants of the Cerrado in Goiás state: Campomanesia adamantium (Cambess.) O. Berg, Protium ovatum (Engl. in Mart.) and Cardiopetalum calophyllum (Schltdl.). Essential oils were extracted from both C. adamantium and C. calophyllum leaves and from P. ovatum leaves and green fruits by hydrodistillation carried out by a Clevenger-type apparatus. The chemical composition of the essential oils was determined by Gas Chromatography coupled to Mass Spectrometry (GC-MS). The following major chemical constituents were identified in the essential oils under investigation: β-myrcene (62.00%), spathulenol (28.78%), germacrene-B (18.27%), β-caryophyllene oxide (16.40%), β-caryophyllene (14.00%), α-pinene (11.30%), viridiflorol (9.99%), limonene (7.30%) and (Z,E)-pharnesol (6.51%). The antiproliferative activity was evaluated in different human tumor cell lines: breast adenocarcinoma (MCF-7), cervical adenocarcinoma (HeLa) and glioblastoma (M059J). A normal human cell line was included (GM07492A, lung fibroblasts). Results showed that essential oils from C. adamantium leaves got the lowest values of IC50 in all strains of tumor cells under evaluation. They were significantly lower than the ones of the normal cell line, an evidence of selectivity. It is worth mentioning that this is the first report of the antiproliferative activity of essential oils from C. adamantium , P. ovatum and C. calophyllum against human tumor cells.

scholarly journals Chemical Composition and Biological Activities of Essential Oils of Pinus patula

2011 ◽  
Vol 6 (10) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Ismail Amri ◽  
Hamrouni Lamia ◽  
Samia Gargouri ◽  
Mohsen Hanana ◽  
Mariem Mahfoudhi ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Biological Activities ◽  
Pathogenic Fungi ◽  
Plant Diseases ◽  
Gas Chromatography Mass Spectrometry ◽  
Pinus Patula ◽  
Flame Ionization ◽  
Total Oil

Essential oils isolated from needles of Pinus patula by hydrodistillation were analyzed by gas chromatography-flame ionization detection (GC-FID) and gas chromatography mass spectrometry (GC-MS). Thirty-eight compounds were identified, representing 98.3% of the total oil. The oil was rich in monoterpene hydrocarbons (62.4%), particularly α-pinene (35.2%) and β-phellandrene (19.5%). The in vitro antifungal assay showed that P. patula oil significantly inhibited the growth of 9 plant pathogenic fungi. The oil, when tested on Sinapis arvensis, Lolium rigidum, Phalaris canariensis and Trifolium campestre, completely inhibited seed germination and seedling growth of all species. Our preliminary results showed that P. patula essential oil could be valorized for the control of weeds and fungal plant diseases.

scholarly journals Chemical constituents of essential oils of Tephrosia purpurea and Ipomoea carnea and their repellent activity against Odoiporus longicollis

2015 ◽  
Vol 80 (4) ◽  
pp. 465-473 ◽  
Author(s):  
Kitherian Sahayaraj ◽  
Poolpandi Kombiah ◽  
Anand Dikshit ◽  
Martin Rathi
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Essential Oils ◽  
Oil Content ◽  
Chemical Constituents ◽  
Repellent Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Hexadecanoic Acid ◽  
Ipomoea Carnea ◽  
Tephrosia Purpurea

Chemical constituents of essential oils (EOs) obtained from stem and root of Tephrosia purpurea (Linn.) Pers. and Ipomoea carnea Jacq. were investigated by Gas Chromatography-Mass Spectrometry (GC-MS). Total lipid and oil content was high in the stem than the root of T. purpurea and I. carnea. Essential oils extracted from the stem and root of T. purpurea and I. carnea showed 9 and 8 compounds respectively. Hexadecanoic acid was found to be the principal constituent of stem (69.61%) and root (46.97%) of T. purpurea while 70.61and 88.89% for stem and root, respectively in the case of I. carnea. The findings of the present study suggest that T. purpurea and I. carnea EOs can be used as a source of hexadecanoic acid which could be used for industrial purposes. The essential oils of T. purpurea and I. carnea showed strong repellent activity for males (-0.73 and -0.70 for T. purpurea and I. carnea stem EO respectively) than females (-0.63 and -0.59 for T. purpurea and I. carnea stem EO respectively) against banana pseudostem weevil Odoiporus longicollis. The results indicated that the active compounds of essential oils from stems of T. purpurea and I. carnea can be explored as natural repellents for control of Odoiporus longicollis.

scholarly journals Chemical composition of essential oils from Bambusa vulgaris Leaf (Fresh and Dried) Schrad. Ex J.C. Wendl. [Poaceae] obtained in Nigeria

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Odunayo Atewolara-odule ◽  
Oseyemi Olubomehin ◽  
Enitan Adesanya ◽  
Adejumoke Hashimi ◽  
Abdulrazaq Ogunmoye
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Essential Oils ◽  
Chemical Constituents ◽  
Gas Chromatography Mass Spectrometry ◽  
Fresh Sample ◽  
Bambusa Vulgaris ◽  
Volatile Aroma ◽  
First Time ◽  
Sulfur Pentafluoride

Essential oil is a hydrophobic concentrated liquid which contains volatile aroma compounds from plants. Bambusa vulgaris grows widely in the tropical and subtropical regions and has been reported to have different ethnomedicinal values such as antimalaria and antioxidant. It is also known to be an ornamental plant. The essential oil was extracted from both the fresh and dried leaves of Bambusa vulgaris by hydrodistillation method using a Clevenger apparatus. The chemical constituents of the essential oils were characterized using gas chromatography and gas chromatography-mass spectrometry. The essential oils obtained are colourless with an herbal odour giving a yield of 0.64 w/w for the fresh sample and 1.08 w/w for the dried sample. A total of thirty-nine (39) compounds were identified from the essential oils of both samples. The major constituents in the fresh leaves were 3-aminodibenzofuran (19.2%), ?-ocimene (11.1%), undecane (9.6%), tridecane (8.6%), [3,2-b] pyridin-6-octahydropyrano (7.1%), 2-mono laurin (6.9%) and vinyl decanoate (5.9%). Prominent compounds of the essential oil in the dried leaves were chlorophenoxymethylenimino sulfur pentafluoride (74.5%) and 2,2,6,6-tetramethyl-3,5-heptanedione (17.3%). Undecane, tridecane, hexadecane and octadecane were found both in the fresh and dried leaves. The chemical constituents of the essential oils from Nigeria Bambusa vulgaris were reported in this study for the first time to the best our knowledge and this could be useful in aromatherapy.

scholarly journals Anti-Oxidant, Anti-Bacterial and Anti-Cancer Activity of Mentha Piperita Against Mcf-7 Cells

2021 ◽  
Vol 14 (3) ◽  
pp. 1685-1693
Author(s):  
Renuka Saravanan ◽  
Ravichandran Natesan ◽  
Sumathi C Samiappan ◽  
Sivakumar Ramalingam
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Bacillus Cereus ◽  
Cell Line ◽  
Bacterial Species ◽  
Mentha Piperita ◽  
Anticancer Activities ◽  
Leaf Extracts ◽  
Apoptotic Effect ◽  
Mcf 7

The study evaluates the antioxidant, antibacterial and anticancer activities of different extracts of Menthapiperitaleaves against the MCF-7 cell line. The leaves of Menthapiperitawere extracted with aqueous, ethyl acetate, and chloroform. These extracts were subjected to qualitative phytochemical screening, antibacterial activity, cytotoxic activity, and AO/ErBr assay for cells' apoptotic effect against the MCF-7 cell line. Qualitative analysis of the leaves' different extracts revealed glycosides, alkaloids, flavonoids, terpenoids, tannin, and saponin. The antibacterial activity of the leaf extracts was examined against four different bacterial species (Bacillus cereus, Pseudomonasfluorescens, Aeromonas hydrophila, and Klebsiella pneumoniae). The aqueous extract exhibited a high level of antibacterial activity (18.66mm ±1.1 in Bacillus cereus).Free‑radicalscavenging activity of chloroform extractof M. piperita leaves was found to be more than aqueous and ethyl acetate extracts. Further, aqueous, ethyl acetate,and chloroform extracts exerted a cytotoxic effect with the IC50value of 45±1.5μg/ml, 29±1.2μg/ml, and 24±1.0μg/ml, respectively.From this study, we have observed that chloroform extract showed a concentration-dependent apoptotic effect against MCF-7 cellsdetermined by AO/EtBr assay.The resultsfurther depicted that the selected traditionalMentha piperita could be used as a potential anticancer, antibacterial, and good antioxidant agent against the MCF-7 cell line.

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