Profile and Drug Susceptibility Pattern of Gram Negative Bacterial Isolates - A Retrospective Institutional Study

This study was conducted in Ayder comprehensive specialized Hospital, Mekelle, Northern Ethiopia, to determine the bacterial profiles and drug susceptibility pattern from body fluids. A total of 218 patients were investigated, of which 146 (67%) were males. The age of the study subjects ranged from 2 days to 80 years with 96(44%) in the age group of 15 years and above. The overall bacterial infection was 44 (20.2 %) of which gram positive bacteria were prevalent, 23 (52.3%) than gram negative bacteria 21 (47.7%). The predominantly isolated bacteria wereS. pneumonia,followed byK.pneumoniae, S. aureus, andE coli.Multidrug resistance was observed in 12 (100%) of the isolated gram positive bacteria and in 6 (75%) of the isolated gram negative bacteria.

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Drug Susceptibility Pattern of Aerobic Bacterial isolates from Pulmonary Infection in HIV Seropositives and their Correlation with CD4 Count

IOSR Journal of Dental and Medical Sciences ◽

10.9790/0853-13363741 ◽

2014 ◽

Vol 13 (3) ◽

pp. 37-41

Author(s):

Shilpa A ◽

◽

Anuradha K ◽

Venkatesha D

Keyword(s):

Pulmonary Infection ◽

Drug Susceptibility ◽

Cd4 Count ◽

Bacterial Isolates ◽

Susceptibility Pattern ◽

Drug Susceptibility Pattern

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Drug susceptibility pattern in organisms isolated during acute exacerbation of Chronic Obstructive Pulmonary Disease

Medical Journal of Shree Birendra Hospital ◽

10.3126/mjsbh.v15i1.14125 ◽

2016 ◽

Vol 15 (1) ◽

Author(s):

Sanjeet Krishna Shrestha ◽

Raina Chaudhary ◽

Sabita Bhatta ◽

Manan Karki ◽

Brajendra Srivastava ◽

...

Keyword(s):

Acute Exacerbation ◽

Drug Susceptibility ◽

Chronic Obstructive ◽

Pseudomonas Sp ◽

Susceptibility Pattern ◽

Gram Positive ◽

Gram Negative ◽

Acute Exacerbations ◽

Drug Susceptibility Pattern ◽

Acinetobacter Sp

AbstractIntroductionDisease process in chronic obstructive lung disease is interrupted by acute exacerbations. Bacterial and viral infections accounts for majority of cases. Gram positive and Gram Negative organism are both associated.Materials and MethodsSputum samples were collected from COPD patients in acute exacerbation, on the day of emergency visit. Drug susceptibility pattern was evaluated for the study population to identify the prevalence of susceptible and resistant organisms.ResultsEighty-nine culture positive sputum samples were processed for drug susceptibility. Gram negative bacilli (88.76%) were isolated more than Gram positive cocci (11.24%). Pseudomonas sp., Acinetobacter sp., Klebseilla sp. and E. coli were the most common Gram negative bacilli. Multi-drug resistance status was identified in higher percents in Acinetobacter sp. (81.25%), Pseudomonas sp. (62.5%) and Klebseilla sp. (46.6%). Aminoglycosides and Quinolones showed good sensitivity to GNBs. However, Carbapenems were found to be the most effective agents against these organisms.ConclusionGram negative infection is common in COPD. Multi drug resistant pathogens are increasingly associated with acute exacerbations. Routinely used antibiotics are becoming less effective.

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Antibiotic Resistance Pattern of Bacterial Isolates from Post- Tracheostomised Patients Attending Tertiary Care Hospital in Nepal

Journal of Institute of Science and Technology ◽

10.3126/jist.v21i1.16064 ◽

2016 ◽

Vol 21 (1) ◽

pp. 129-132 ◽

Cited By ~ 1

Author(s):

Eliza Thapa ◽

Roshan Thapa ◽

Anjana Singh ◽

Bharat Mani Pokharel ◽

Upendra Devkota

Keyword(s):

Klebsiella Oxytoca ◽

Tertiary Care ◽

Drug Susceptibility ◽

Diffusion Method ◽

Resistance Pattern ◽

Resistant Bacteria ◽

Care Hospital ◽

Bacterial Isolates ◽

Susceptibility Pattern ◽

Drug Susceptibility Pattern

The bacterial profile and their drug susceptibility pattern was studied in post-tracheostomised patients admitted in National Institute of Neurological and Allied Sciences. Identification of organisms was done by standard microbiological techniques and antibiogram was performed by Kirby Bauer disc diffusion method according to Clinical and Laboratory standard (CLSI) guidelines. Significant growth was observed in 58.6% samples with polymicrobial growth in 19.5% samples among the total positive cultures. Pseudomonas aeruginosa was the predominant organism (34.2%) followed by Acinetobacter spp. (31.8%), Escherichia coli (8.9%), Klebsiella pneumoniae (6.0%), Citrobacter fruendii (5.6%), Klebsiella oxytoca (4.0%), Providencia spp. and Staphylococcus aureus (2.8% each), Proteus mirabilis (2.4%) and Proteus vulgaris (1.2%) . More than 90% of Gram negative bacterial isolates were found resistant to Ampicillin, Cefixime, Cephotaxime. The most effective drugs against S. aureus were Vancomycin, Rifampicin, Amoxycillin and Clavulanic acid showing 100% sensitivity. Highest resistance rate was observed for Ampicillin (85.71%) and low for Gentamicin (14.28%). Findings of this study show emerging threat of multidrug resistant bacteria. Thus, periodic monitoring and assessing drug susceptibility pattern of bacteria and rational use of antibiotics in post tracheostomised patients were recommended.Journal of Institute of Science and TechnologyVolume 21, Issue 1, August 2016, page: 129-132

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Drug Susceptibility pattern of Organisms Isolated During Acute Exacerbation of Chronic Obstructive Pulmonary Disease in a Tertiary Level Hospital of Nepal

Medical Journal of Shree Birendra Hospital ◽

10.3126/mjsbh.v15i1.14945 ◽

2016 ◽

Vol 15 (1) ◽

pp. 43-51

Author(s):

Sanjeet Krishna Shrestha ◽

Raina Chaudhary ◽

Sabita Bhatta ◽

Manan Karki ◽

Brajendra Srivastava ◽

...

Keyword(s):

Acute Exacerbation ◽

Drug Susceptibility ◽

Chronic Obstructive ◽

Pseudomonas Sp ◽

Susceptibility Pattern ◽

Gram Positive ◽

Gram Negative ◽

Acute Exacerbations ◽

Drug Susceptibility Pattern ◽

Acinetobacter Sp

Introduction: Disease process in chronic obstructive lung disease is interrupted by acute exacerbations. Bacterial and viral infections account for majority of cases. Gram positive and Gram negative organism both are associated with exacerbations. Methods: Sputum samples were collected from COPD patients in acute exacerbation, on the day of emergency visit. Drug susceptibility pattern was evaluated for the study population to identify the prevalence of susceptible and resistant organisms. Results: Eighty-nine culture positive sputum samples were processed for drug susceptibility. Gram negative bacilli (88.76%) were isolated more than Gram positive cocci (11.24%). Pseudomonas sp., Acinetobacter sp., Klebseilla sp. and E. coli were the most common Gram negative bacilli. Multi-drug resistance status was identified in higher percents in Acinetobacter sp. (81.25%), Pseudomonas sp. (62.5%) and Klebseilla sp. (46.6%). Aminoglycosides and Quinolones showed good sensitivity to GNBs. However, Carbapenems were found to be the most effective agents against these organisms. Conclusion: Gram negative infection is common in COPD. Multi drug resistant pathogens are increasingly associated with acute exacerbations. Routinely used antibiotics are becoming less effective.

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Bacterial Isolates and its Antibiotic Susceptibility Pattern in NICU

Kathmandu University Medical Journal ◽

10.3126/kumj.v11i1.11030 ◽

2014 ◽

Vol 11 (1) ◽

pp. 66-70 ◽

Cited By ~ 13

Author(s):

S Shrestha ◽

NC Shrestha ◽

S Dongol Singh ◽

RPB Shrestha ◽

S Kayestha ◽

...

Keyword(s):

Intensive Care Unit ◽

Intensive Care ◽

Neonatal Intensive Care Unit ◽

Neonatal Intensive Care ◽

Bacterial Isolates ◽

Susceptibility Pattern ◽

Gram Positive ◽

Antibiotic Susceptibility Pattern ◽

Gram Negative ◽

Nosocomial Sepsis

Background Neonatal sepsis is one of the major causes of morbidity and mortality among the newborns in the developing world. Objectives To determine the common bacterial isolates causing sepsis in neonatal intensive care unit and its antibiotic susceptibility pattern. Methods A one year discriptive prospective study was conducted in neonatal intensive care unit to analyse the results of blood culture and to look into the sensitivity of the commonly used antibiotics. Results The blood culture yield by conventional method was 44.13% with nosocomial sepsis accounting for 10.79%. 84.08% were culture proven early onset sepsis and 15.95% were late onset sepsis. Klebsiella infection was the commonest organism isolated in early, late and nosocomial sepsis but statistically not significant. Gram positive organisms were 39.36% in which Staphylococcus aureus was the leading microorganism followed by coagulase negative staphylococcus areus. Gram negative organisms were 60.64% amongst them Klebsiella was the most often encountered followed by Pseudomonas. The most common organism Klebsiella was 87.5% and 78.3% resistance to ampicillin and gentamycin respectively. Among gram negative isolates 87.5% and 77.2% were resistance to ampicillin and gentamycin respectively. Among gram positive isolates 58.5% and 31.5% resistance were noted to ampicillin and gentamycin respectively. Resistance to cefotaxim to gram negative and gram positive isolates were 87.34% and 59.35% respectively. Conclusion Klebsiella is most common organism which is almost resistance to first line antibiotics. Resistance to both gram negative and gram positive isolates among firstline antibiotics and even with cefotaxim is emerging and is a major concern in neonatal intensive care unit. DOI: http://dx.doi.org/10.3126/kumj.v11i1.11030 Kathmandu University Medical Journal Vol.11(1) 2013: 66-70

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Group B streptococci vaginal colonization and drug susceptibility pattern among pregnant women attending in selected public antenatal care centers in Addis Ababa, Ethiopia

BMC Pregnancy and Childbirth ◽

10.1186/s12884-018-1791-4 ◽

2018 ◽

Vol 18 (1) ◽

Cited By ~ 3

Author(s):

Solomon Assefa ◽

Kassu Desta ◽

Tsehaynesh Lema

Keyword(s):

Antenatal Care ◽

Pregnant Women ◽

Addis Ababa ◽

Drug Susceptibility ◽

Susceptibility Pattern ◽

Group B Streptococci ◽

Vaginal Colonization ◽

Drug Susceptibility Pattern ◽

Group B

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Bacterial Isolates and Their Antibiotics Susceptibility Pattern Among Patients Admitted With Chronic Lower Limb Ulcers at Kilimanjaro Christian Medical Centre Northern Zone Referral Hospital, Tanzania

The International Journal of Lower Extremity Wounds ◽

10.1177/1534734620923455 ◽

2020 ◽

pp. 153473462092345

Author(s):

Wilson Marco Hape ◽

Mujuni Josephat Magambo ◽

James Samwel Ngocho ◽

Victor Mosha ◽

Sia E. Msuya ◽

...

Keyword(s):

Lower Limb ◽

Culture Method ◽

Medical Centre ◽

Resistance Pattern ◽

Bacterial Isolates ◽

Susceptibility Pattern ◽

Cross Sectional ◽

Gram Negative ◽

Kilimanjaro Christian Medical Centre ◽

Antibiotics Susceptibility

Objective. To identify the bacterial isolates and their antibiotics susceptibility pattern among patients with lower limb ulcers admitted at a tertiary hospital in northern Tanzania. Methodology. A cross-sectional study was conducted between April and July 2018 at the Kilimanjaro Christian Medical Centre. Questionnaire was used to obtain the demographics and clinical information of participants. Wound samples were collected and culture method used to identify the bacteria and their susceptibility patterns. Results. Out of 65 participants, 55 (84.6%) had positive aerobic bacterial growth. Twelve (18 %) participants had more than one bacterium. Fifty-nine (88.1%) were gram-negative bacteria. Proteus vulgaris (13, 19.9%), Pseudomonas spp (10, 14.8%), and Staphylococcus aureus (8, 11.7%) were the common isolates. Out of 59 gram-negative bacterial, 47 (78%) were sensitive to amikacin. Staph were sensitive to oxacillin (75%; n = 8) and vancomycin (50%; n = 8). Gram-negative were common isolates and were resistant to amoxicillin/clavulanic acid but sensitive to amikacin. Conclusion. The observed resistance to antibiotics calls for continuous monitoring of the resistance pattern to guide the empirical management of patients with leg ulcer.

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