scholarly journals Comparison of PCR with stool culture for diagnosis of Asymptomatic Typhoid Carriesin stool samples collected in a tertiary care hospital in East India

2018 ◽  
Vol 6 (9) ◽  
Author(s):  
Suneel Kumar Ahirwar ◽  
Keyword(s):  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Stool Culture ◽  
Care Hospital ◽  
Stool Samples

scholarly journals Rotavirus in diarrhoeic children under-five attending a tertiary care hospital in Mumbai, India

2018 ◽  
Vol 5 (5) ◽  
pp. 1863
Author(s):  
Nikhil Khude ◽  
Lona Dash ◽  
Sushma Save ◽  
Badhuli Samal ◽  
Jayanthi Shastri
Keyword(s):  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Acute Diarrhoea ◽  
Enzyme Linked Immunosorbent Assay ◽  
Care Hospital ◽  
Etiological Diagnosis ◽  
Under Five ◽  
Under Five Children ◽  
Stool Samples ◽  
Parasitic Pathogens

Background: Diarrhoeal diseases are a leading cause of morbidity and mortality among children requiring hospitalization in developing countries. Rotavirus is the most common cause of acute diarrhoea among under-five children. Etiological diagnosis of diarrhoea would enable appropriate management of patients while limiting the spread of drug resistant pathogens. This study was undertaken to determine presence of rotavirus and other diarrhoeal pathogens in under-five diarrhoeic children attending a tertiary care hospital, and the related clinical presentations.Methods: 120 stool samples of under-five children with acute diarrhoea, attending the OPD and indoor services of a tertiary care hospital, were studied over one year. Rotavirus antigen was detected using enzyme linked immunosorbent assay. Bacterial and parasitic pathogens were detected using standard microbiological techniques.Results: Out of 120 stool samples tested, 36 were positive for Rota virus antigen. Bacterial isolates included E. coli (25), Vibrio cholerae (12) and Aeromonas species (1). Parasites were observed in nine samples and multiple pathogens in nine.Conclusions: Rotavirus continues to be a major cause of childhood diarrhoea. As antibiotics have no role in the management of viral and parasitic diarrhoeas, etiological diagnosis is imperative for proper management of diarrhoea and prevention of indiscriminate use of antibiotics.

scholarly journals Prevalence of Intestinal Parasitic Infestations among Children in a Tertiary Care Centre

2021 ◽  
pp. 882-886
Author(s):  
J. S. Danish ◽  
P. Vasanth ◽  
V. Subramanian
Keyword(s):  
Entamoeba Histolytica ◽  
Giardia Lamblia ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Parasitic Infections ◽  
Care Hospital ◽  
Tertiary Care Centre ◽  
Pediatric Age Group ◽  
Stool Samples ◽  
One Year

Introduction: Intestinal parasitic infestations are more common in developing countries and an important cause of morbidity in pediatric age group. It causes abdominal pain, diarrhoea, anemia, impaired cognitive function, growth retardation and poor academic performance among school going children. Objectives: To analyze age, gender and most common parasitic infestations among children in a tertiary care hospital in south India. Materials and Methods: Stool samples of pediatric patients in a tertiary care hospital is analysed for a period of one year for detection of prevalence of parasitic infestations. Results: In our study, 115 stool samples were analysed and prevalence of intestinal parasitic infections was found to be 7.82% (9/115).There were five different parasites encountered. The most common parasite identified was Giardia lamblia(4/9)(44.44%) followed by Entamoeba histolytica(2/9)(22.22%), Ascarislumbricoides(1/9)(11.11%), Trichuris trichiura(1/9)(11.11%), and Hymenolepis nana(1/9)(11.11%). Conclusion: Giardia lamblia and Entamoeba histolytica were found to be the most common organism in our study. Awareness about proper hygiene, sanitation and prophylactic anti-protozoal medications is important to prevent the occurrence and complications of intestinal parasitic infestations.

scholarly journals Etiology of Infectious Diarrhea in Patients Tested for Clostridium difficile: If It Isn’t Clostridium difficile, What Is It?

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S2-S2
Author(s):  
Jennie H Kwon ◽  
Tiffany Hink ◽  
Kimberly Reske ◽  
Erik R Dubberke ◽  
Carey-Ann D Burnham
Keyword(s):  
Clostridium Difficile ◽  
Tertiary Care ◽  
Agar Plate ◽  
Stool Culture ◽  
Blood Agar Plate ◽  
Care Hospital ◽  
E Coli ◽  
Toxigenic Culture ◽  
Stool Samples ◽  
Research Grant

Abstract Background The objective of the study was to assay for alternative infectious causes of diarrhea in patients with negative EIA tests for Clostridium difficile. Methods A hard-stop alert was implemented at a tertiary care hospital to limit repeat testing for C. difficile within 96 hours of an initial negative EIA. Stool samples from patients with a negative (–) repeat EIA test for C. difficile within 96 hours in the 3 months pre- and postintervention underwent further evaluation: C. difficile toxigenic culture, GeneXpert C. difficile PCR, Biofire Gastrointestinal (GI) Panel, and culture on a blood agar plate. Results Of the 84 C. difficile EIA stool specimens evaluated, 8% were toxigenic culture positive (+), 8% tested + for C. difficile via the Biofire GI panel, and 5 (7%) + with the GenXpert C. difficile PCR (Table 1). Three of these patients were diagnosed with CDI within 30 days of a + test. Five patients were + for Norovirus via Biofire GI panel; none were tested for or diagnosed with Norovirus. Two patients were + for Enteropathogenic E. coli and one for Enteroaggregative E. coli via Biofire GI panel; none were tested for or diagnosed with E. coli infection. One patient was positive for Salmonella and Salmonella was isolated by stool culture. Conclusion Patients tested for C. difficile may have alternate causes of diarrhea. When evaluating hospitalized patients with diarrhea, C. difficile, along with alternate causes of diarrhea can be considered. Disclosures E. R. Dubberke, Merck: Consultant, Consulting fee; Biofire: one time talk, Speaker honorarium;; Alere: one-time talk, Speaker honorarium; Sanofi pasteur: Grant Investigator, Grant recipient; Pfizer: Consultant, Consulting fee; Rebiotix: Investigator, Research support; Rebiotix: Consultant, Consulting fee; valneva: Consultant, Consulting fee; C. A. D. Burnham, bioMerieux: Grant Investigator, Research grant; ThermoFisher: Consultant, Salary; Cepheid: Grant Investigator, Research grant

Confidence toward vaccination as reported by parents of children admitted to a tertiary care hospital in Riyadh, Saudi Arabia: A cross sectional study

Vacunas ◽  
2020 ◽  
Vol 21 (2) ◽  
pp. 95-104 ◽  
Author(s):  
Y.M. AlGoraini ◽  
N.N. AlDujayn ◽  
M.A. AlRasheed ◽  
Y.E. Bashawri ◽  
S.S. Alsubaie ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Cross Sectional Study ◽  
Care Hospital ◽  
Sectional Study ◽  
Cross Sectional

Abstract #1180: Trimester Specific Reference Interval for Thyroid Hormones During Pregnancy at a Tertiary Care Hospital in Haryana, India.

2016 ◽  
Vol 22 ◽  
pp. 293
Author(s):  
Rajesh Rajput ◽  
Bhagat Singh ◽  
Vasudha Goel ◽  
Amit Verma ◽  
Shashi Seth ◽  
...  
Keyword(s):  
Thyroid Hormones ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Reference Interval ◽  
Specific Reference ◽  
Care Hospital
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