scholarly journals INCIDÊNCIA DE FUNGOS ASSOCIADOS A SEMENTES DE SOJA TRANSGÊNICA VARIEDADE BRS VALIOSA RR

2013 ◽  
Vol 3 (1) ◽  
pp. 52 ◽  
Author(s):  
Thatyane Pereira de Sousa ◽  
Ivaneide Oliveira Nascimento ◽  
Claudio Belmino Maia ◽  
Joacir Morais ◽  
Gustavo De Andrade Bezerra ◽  
...  

RESUMO: A utilização de sementes de boa qualidade sanitária no plantio é um fator de fundamental importância para o estabelecimento da lavoura, uma vez que a sementes pode servir como fonte de inoculo. Com o presente trabalho se objetivou identificar os principais fungos associados às sementes de soja transgênica variedade BRS Valiosa RR. A análise sanitária foi conduzida no Laboratório de Fitopatologia do Centro de Estudos Superiores de Imperatriz da Universidade Estadual do Maranhão, utilizando-se o “blotter test” de acordo com as Regras para analise de Sementes, do Ministério da Agricultura. As sementes foram incubadas em condições de fotoperíodo por sete dias, com incidência de luz branca fluorescente num intervalo de 12 horas de luz e 12 horas de escuro, à temperatura de aproximadamente 26±5 0C, durante sete dias. Após esse período as sementes foram examinadas individualmente, sob microscópio estereoscópico e óptico e os resultados foram expressos em percentagem de incidência de fungos. Foram identificados cinco gêneros de fungos: Aspergillus flavus, Aspergillus niger, Rizopus sp., Colletotrichum truncatum, Fusarium semitectum, Penicillium sp. Os quais podem afetar o desenvolvimento de plântulas no campo. PALAVRAS-CHAVE: patógenos, sanidade, qualidade.

2020 ◽  
Vol 3 (1) ◽  
pp. 1-15
Author(s):  
Sifa Sobianti ◽  
Loekas Soesanto ◽  
Suciati Hadi

Penelitian ini bertujuan untuk mengetahui (1) beragam spesies jamur patogen tular-benih pada lima varietas padi, dan (2) persentase daya kecambah lima varietas padi. Penelitian dilaksanakan di Laboratorium Karantina Pertanian Kelas I Semarang, dari bulan Maret sampai dengan Mei 2019. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 5 perlakuan dan 5 ulangan, perlakuan terdiri atas Varietas Inpago Unsoed 1, Inpago Unsoed Parimas, IR64, Ciherang, dan Situ Bagendit. Isolasi dan identifikasi jamur patogen tular-benih dilakukan pada blotter test dan medium PDA, dengan pengamatan di bawah mikroskop stereo dan kompon, kemudian dibandingkan dengan pustaka. Variabel yang diamati yaitu morfologi koloni patogen, morfologi patogen, dan daya kecambah. Hasil penelitian menunjukkan bahwa (1) pada semua varietas benih padi terdapat jamur patogen tular-benih, tetapi tidak semua jamur tersebut dijumpai pada setiap varietas. Jenis jamur patogen tular-benih yang dijumpai, yaitu Alternaria padwickii Ganguly, Aspergillus flavus Link, Aspergillus niger van Tieghem, Curvularia lunata (Wakker) Boedjin, Curvularia pallescens Boedjin, Drechslera oryzae Breda de Haan, Fusarium semitectum Berk. & Rav. [W&R, G,B,J], Rhizoctonia solani J. G. Kuhn, Rhizopus oryzae Went & Prins. Geerl., dan Tilletia barclayana Bref., dan (2) uji daya kecambah menunjukkan bahwa masing-masing varietas benih padi memilki persentase daya kecambah benih yang berbeda. Daya kecambah benih berturut-turut pada varietas IR64, Ciherang, Inpago Unsoed 1, Inpago Unsoed Parimas, dan Situ Bagendit sebesar 85,75, 81,75, 80,25, 76,5, dan 70%.


2020 ◽  
Vol 29 (2) ◽  
pp. 183-189
Author(s):  
Nusrat Jahan Shova ◽  
Shamim Shamsi ◽  
MA Bashar

Two major species of Indian spinach, namely Basella alba L. and B. rubra L. were selected to find out associated mycoflora and their pathogenic potentiality. Ten fungi viz., Alternaria alternata, Aspergillus flavus, A. fumigatus, A. niger, Colletotrichum dematium, C. lindemuthianum, Curvularia lunata, Drechslera sacchari, Fusarium semitectum and Penicillium sp. were found to be associated with the selected leaves of Basella spp. Among the isolated fungi C. lindemuthianum, D. sacchari and F. semitectum were found to be pathogenic to Basella spp. Association of C. lindemuthianum and D. sacchari with Basella spp. are recorded first time from Bangladesh. Dhaka Univ. J. Biol. Sci. 29(2): 183-189, 2020 (July)


Author(s):  
S. Apuri ◽  
C. Kwoseh ◽  
E. A. Seweh ◽  
I. O. Tutu

Aims: The study aims to identifying the microorganisms associated with post-harvest rot of frafra potatoes in Bongo-soe, Upper east region of Ghana. Place and Duration of Study: Department of Horticulture and the Pathology laboratory of the Faculty of Agriculture, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana. The Solenostemon rotundifolius tubers were stored at the Horticulture Department laboratory whiles rot identification was carried out at the Pathology laboratory of the Faculty of Agriculture. The Tubers were stored from 2nd November, 2012 to 22nd March 2013. Methodology: Four hundred (400) tubers of black cultivar and four hundred (400) tubers of a brown cultivar of Solenostemon rotundifolius tubers showing visible signs of rot during the storage were collected. Pieces of diseased tissues from the margin of the necrotic collected and immersed in 10% commercial bleach solution for sterilisation, for one minute. These were then blotted dry and plated on Potato Dextrose Agar PDA. The plates were sealed with a cellotape until growth occurred. Results: The microorganisms identified to be responsible for causing rot in Solenostemon rotundifolius tubers were six in number. Colletotrichum gloeosporioides was identified to be responsible for 30.76% of rots observed, followed by Aspergillus niger, 23.07%, Curvularia lunata, 19.23%, Aspergillus flavus, 11.54%, Trichoderma sp and Penicillium sp both recorded 7.70% of rots observed. The percentage incidence of Aspergillus niger (15.38%), Curvularia lunata (11.54%) and Aspergillus flavus (7.69%) was higher in the black cultivar as compared with the brown cultivar which had percentage incidence of 7.69%, 7.69% and 3.85% respectively. Also, the percentage incidence of Colletotrichum gloeosporioides (15.38%) and Penicillium sp (3.85%) was the same in both the black and brown cultivars of Solenostemon rotundifolius tubers used in this study. Conclusion: The activities of the damaging microorganisms can be reduced by controlling mechanical injury during harvesting, transportation and storage of Solenostemon rotundifolius tubers should be prevented or reduced because they pave the way for tuber infection by the rot causing microorganisms.


2018 ◽  
Vol 27 (1) ◽  
pp. 93-100
Author(s):  
Farhana Islam ◽  
Shamim Shamsi ◽  
Md Abul Bashar

A total of ten fungal species were isolated from the anthracnose symptoms of three varieties of Mangifera indica L. viz., Himsagar, Langra and Amrapali. The isolated fungi were Alternaria alternata (Fr.) Keissler, Aspergillus flavus Link, A. fumigatus Fresenius, A. niger van Tiegh., Colletotrichum gloeosporioides (Penz.) Sacc., Fusarium semitectum Berk. & Rav., Papulaspora sp. Preuss., Penicillium sp. Link., Pestalotiopsis guepinii (Desm.) Stey. and Rhizopus stolonifer (Ehrenb.) Vull. Colletotrichum gloeosporioides showed the highest per cent (26.82) frequency in Himsagar whereas R. stolonifer showed the lowest (2.22). Pestalotiopsis guepinii showed the highest per cent (46.83) frequency in Langra whereas R. stolonifer showed the lowest (1.17). Colletotrichum gloeosporioides showed the highest per cent (38.67) frequency in Amrapali whereas Papulaspora sp. showed the lowest (4.00). Among the isolated fungi C. gloeosporioides, F. semitectum and P. guepinii were found to be pathogenic to three mango varieties. Association of Fusarium semitectum, Papulaspora sp. and Pestalotiopsis guepinii with mango is new record in Bangladesh. Dhaka Univ. J. Biol. Sci. 27(1): 93-100, 2018 (January)


2015 ◽  
Vol 24 (2) ◽  
pp. 137-145
Author(s):  
Romana Akter ◽  
KS Hossain ◽  
MA Bashar

Antagonistic potentials of seven rhizoshere soil fungi viz., Aspergillus flavus Link., A. fumigatus Fresen., A. niger Tiegh., A. terreus Thom., Penicillium sp., Trichoderma harzianum Refat. and T. viride Pers. were tested against the Fusarium oxysporum Schlecht. and F. solani (Mart.) Sacc. – the two pathogens, isolated from wilted roots of brinjal plants. Out of seven soil fungi Trichoderma harzianum was found most effective to control the growth of both the test pathogens. More than 50% inhibition of the test pathogens were observed with T. viride, Aspergillus niger and A. fumigatus in colony interaction. The maximum inhibition of radial growth of the test pathogens was observed with volatile metabolites and cell free culture filtrates of Trichoderma harzianum. This fungus may be exploited commercially to control the disease. Dhaka Univ. J. Biol. Sci. 24(2): 137-145, 2015 (July)


2020 ◽  
Vol 3 (1) ◽  
pp. 1-15
Author(s):  
Sifa Sobianti ◽  
Loekas Soesanto ◽  
Suciati Hadi

Penelitian ini bertujuan untuk mengetahui (1) beragam spesies jamur patogen tular-benih pada lima varietas padi, dan (2) persentase daya kecambah lima varietas padi. Penelitian dilaksanakan di Laboratorium Karantina Pertanian Kelas I Semarang, dari bulan Maret sampai dengan Mei 2019. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 5 perlakuan dan 5 ulangan, perlakuan terdiri atas Varietas Inpago Unsoed 1, Inpago Unsoed Parimas, IR64, Ciherang, dan Situ Bagendit. Isolasi dan identifikasi jamur patogen tular-benih dilakukan pada blotter test dan medium PDA, dengan pengamatan di bawah mikroskop stereo dan kompon, kemudian dibandingkan dengan pustaka. Variabel yang diamati yaitu morfologi koloni patogen, morfologi patogen, dan daya kecambah. Hasil penelitian menunjukkan bahwa (1) pada semua varietas benih padi terdapat jamur patogen tular-benih, tetapi tidak semua jamur tersebut dijumpai pada setiap varietas. Jenis jamur patogen tular-benih yang dijumpai, yaitu Alternaria padwickii Ganguly, Aspergillus flavus Link, Aspergillus niger van Tieghem, Curvularia lunata (Wakker) Boedjin, Curvularia pallescens Boedjin, Drechslera oryzae Breda de Haan, Fusarium semitectum Berk. & Rav. [W&R, G,B,J], Rhizoctonia solani J. G. Kuhn, Rhizopus oryzae Went & Prins. Geerl., dan Tilletia barclayana Bref., dan (2) uji daya kecambah menunjukkan bahwa masing-masing varietas benih padi memilki persentase daya kecambah benih yang berbeda. Daya kecambah benih berturut-turut pada varietas IR64, Ciherang, Inpago Unsoed 1, Inpago Unsoed Parimas, dan Situ Bagendit sebesar 85,75, 81,75, 80,25, 76,5, dan 70%.


Author(s):  
Larissa Nobre Veras ◽  
Erivan de Olivindo Cavalcante ◽  
Jacqueline Moura Barbosa ◽  
Thyra Pimentel Alves ◽  
José Mauro da Silva Alves ◽  
...  

O amendoim é uma oleaginosa originária da América do Sul. A espécie utilizada na alimentação humana, Arachis hypogaea, é bastante comercializada na forma industrializada ou caseira. Este estudo visou avaliar a contaminação micológica de amostras de amendoim industrializados e caseiros, determinando as possíveis interferências e os malefícios para a saúde humana. Para a realização do presente estudo, obteve-se a coleta de três amostras industrializadas, comercializadas em supermercados, e três amostras caseiras, vendidas por ambulantes, ambas encontradas na cidade de Fortaleza, Ceará. Elas foram levadas ao Laboratório de Microbiologia na Universidade Estadual do Ceará e os procedimentos para análise micológica foram realizados pelo método de plaqueamento. Todas as amostras analisadas em triplicada, tanto caseiras quanto industrializadas, apresentaram crescimento fúngico, constatando-se, em geral, a presença dos seguintes gêneros e espécies: Aspergillus flavus, Aspergillus terreus, Aspergillus niger, Rhizopus sp. e Penicillium sp. Os achados fúngicos podem estar relacionados a micoses, além da produção de aflatoxinas prejudiciais à saúde humana. Concluiu-se que os amendoins analisados apresentaram uma ampla contaminação fúngica, podendo ser responsáveis pelo comprometimento da saúde dos consumidores. Consequentemente, aconselha-se um maior controle nos processos de manipulação e armazenamento de amendoins, objetivando a garantia da segurança alimentar da população.


2012 ◽  
Vol 36 (1) ◽  
pp. 143-146
Author(s):  
Shamim Shamsi ◽  
Razia Sultana ◽  
Yasmin Fatema

Nine species of fungi belonging to eight genera of Deuteromycetes were found associated with leaves and fruits of breadfruit, Artocarpus altilis. The fungi were Alternaria alternata (Fries) Keissler, Aspergillus niger Van Tiegh, Aspergillus flavus Link, Colletotrichum gloeosporioides (Penz.) Sacc, Curvularia lunata (Wakker) Boedijn., Penicillium sp. Pteroconium state of   Apiospora camptospora Penz. & Sacc. and Spicaria sp., and Zygosporium oscheoides Mont.. This is the first report of association of A. alternata, C. gloeosporioides., Curvularia lunata,  Penicillium sp. Pteroconium state of A. camptospora, Spicaria sp. and Z. oscheoides, with breadfruit. Colletotrichum gloeosporioides was the predominating fungus recorded on all infected matured leaves showing brown lesion and anthracnose symptom, and also pathogenic to breadfruit plant. DOI: http://dx.doi.org/10.3329/jbas.v36i1.10930 Journal of Bangladesh Academy of Sciences, Vol. 36, No. 1, 143-146, 2012


EPISTEMUS ◽  
2016 ◽  
Vol 10 (20) ◽  
pp. 14-20
Author(s):  
Gilberto García Navarrete ◽  
Griselda Adilene Sánchez Cubedo ◽  
Sylvia Lorenia López Mazón ◽  
María Elena Ochoa Landín ◽  
Virgilio Rubio Nieblas

En este trabajo se evaluó la presencia de hongos en el aire de aulas del Departamento de Ingeniería Química y Metalurgia, recolectándose 90 muestras utilizando el método pasivo en cajas Petri de 90 mm de diámetro, que contenían Agar Sabouraud Dextrosa. Para identificar el tipo de hongo se preparó un frotis, de acuerdo a la técnica de Impronta. Se determinó la forma y ordenamiento característico de las esporas. Los hongos encontrados fueron Alternaria sp., Aspergillus flavus, Aspergillus niger, Cladosporium sp., Drechslera sp., Penicillium sp., y Fusarium sp. De acuerdo al número de unidades formadoras de colonias (UFC) registradas durante los días de monitoreo, los mayores recuentos se encontraron en el turno matutino, antes del inicio de actividades. Además, se observó que el muestreo matutino del día Lunes presentó la mayor cantidad de UFC en las diferentes aulas, lo cual se atribuye principalmente a la inactividad en el fin de semana.


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