Monitoring the pathogens (Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumannii, Stenotrophomonas maltophilia) of healthcare-associated infections in a hospital

We evaluated isolates of Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii that were reported to the National Healthcare Safety Network from January 2006 through December 2008 to determine the proportion that represented multidrug-resistant phenotypes. The pooled mean percentage of resistance varied by the definition used; however, multidrug resistance was relatively common and widespread.

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Avaliação da contaminação microbiana de cateteres venosos usados em pacientes hospitalizados.

Revista de Ciências Médicas e Biológicas ◽

10.9771/cmbio.v16i2.22152 ◽

2017 ◽

Vol 16 (2) ◽

pp. 167

Author(s):

Cristiane Coimbra De Paula ◽

Lisiane Vieira Paludetti ◽

Walkiria Shimoya-Bittencourt

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Klebsiella Pneumoniae ◽

Acinetobacter Baumannii ◽

Stenotrophomonas Maltophilia ◽

Terapia Intensiva

Introdução: as unidades de terapia intensiva frequentemente utilizam dispositivos invasivos, como os cateteres, os quais podem desencadear complicações como infecção e outros efeitos colaterais que são de grande importância na terapia clínica. Além disso, os cateteres venosos utilizados principalmente em unidades de terapia intensiva contribuem para disseminação de infecção hospitalar. Objetivo: avaliar os microrganismos causadores de infecções em ponta de cateter venoso usado nos pacientes hospitalizados na Santa Casa de Misericórdia de Cuiabá-MT. Metodologia: foi realizado um estudo transversal de natureza clínica, incluindo pacientes que tinham cateter venoso e excluídos os pacientes com sonda vesical. Foi utilizada a Técnica Semi quantitativa de Maki para cultivo e após o período de incubação, as placas com crescimento igual ou superior a 15 UFC, foram submetidas à identificação dos microrganismos através de provas bioquímicas. Resultados: foram analisadas 1.577 pontas de cateteres no ano de 2008, destas, 297 (18,8%) estavam infectadas, cujos microrganismos de maior prevalência foram em 46 (15,5%) pontas a presença de Escherichia coli, 59 (19,9%) da Pseudomonas aeruginosa, 43(14,5%) da Klebsiella pneumoniae, 42 (14,1%) de Staphylococcus sp coagulase negativa e 20 (6,7%) amostras apresentavam Staphylococcus aureus, dentre outros. Das 177 amostras de ponta de cateter analisadas em 2015, 45 (25,4%) estavam infectadas. Foram encontrados em 13 pontas (28,9%) a presença da bactéria Staphylococcus sp coagulase negativa e 8 (17,8%) da Pseudomonas aeruginosa, 5 (11,1%) da Klebsiella pneumoniae, 5 (11,1%) de Stenotrophomonas maltophilia, 4 (8,9%) de Acinetobacter baumannii Conclusão: pacientes internados podem ser expostos a cateteres venosos com significativo grau de contaminação microbiana

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Rapid genotyping of Achromobacter xylosoxidans, Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa and Stenotrophomonas maltophilia isolates using melting curve analysis of RAPD-generated DNA fragments (McRAPD)

Research in Microbiology ◽

10.1016/j.resmic.2011.02.002 ◽

2011 ◽

Vol 162 (4) ◽

pp. 386-392 ◽

Cited By ~ 17

Author(s):

Pieter Deschaght ◽

Leen Van Simaey ◽

Ellen Decat ◽

Els Van Mechelen ◽

Sylvain Brisse ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Klebsiella Pneumoniae ◽

Acinetobacter Baumannii ◽

Stenotrophomonas Maltophilia ◽

Melting Curve ◽

Curve Analysis ◽

Achromobacter Xylosoxidans ◽

Melting Curve Analysis ◽

Dna Fragments

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ХАРАКТЕРИСТИКА И УРОВНИ ВЫЯВЛЕНИЯ БАКТЕРИАЛЬНЫХ ПАТОГЕНОВ У БОЛЬНЫХ ВНЕБОЛЬНИЧНОЙ ПНЕВМОНИЕЙ В ЗАВИСИМОСТИ ОТ СРОКОВ ИХ ПРЕБЫВАНИЯ СТАЦИОНАРЕ

10.21055/preprints-3111946 ◽

2021 ◽

Author(s):

Альбина Бондаренко ◽

Влада Шмыленко ◽

Ольга Троценко ◽

Наталья Пшеничная ◽

Елена Базыкина ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Candida Albicans ◽

Klebsiella Pneumoniae ◽

Acinetobacter Baumannii ◽

Stenotrophomonas Maltophilia ◽

E Coli ◽

Acinetobacter Baumannii Complex

Представлены состав, характеристика лекарственной устойчивости и уровни выявления бактериальных патогенов в пробах мокроты 82 больных с внебольничной пневмонией в зависимости от сроков их пребывания в стационаре: 44 человека обследованы в 1-3 день, 17 человек –на 4-10 день, 21 пациент на 11-22 день. В целом, бактериальная флора выявлена у 64 из 82 наблюдаемых больных (78,0±4,6%). Значимые грамотрицательные патогены (Klebsiella pneumoniae, Escherichiae coli, Acinetobacter baumannii complex, Pseudomonas aeruginosa, Stenotrophomonas maltophilia) выделяли в анализируемый период среди больных со средней степенью тяжести болезни с незначительной частотой (7,3±2,9%; 2,4±1,7%; 3,7±2,1%; 2,4±1,7%; 1,2±1,2%, соответственно возбудителям). Лекарственноустойчивые формы K. pneumoniae, E. coli – продуценты БЛРС, выделяли исключительно на поздних сроках пребывания больных в стационаре. Возбудители из группы неферментирующих грамотрицательных бактерий (A. baumannii complex, P. aeruginosa, S. maltophilia) были выявлены как на ранних, так и на поздних сроках госпитализации. Однако выделители штаммов-продуцентов карбапенемаз A. baumannii complex прошли длительный курс (до 1 месяца) амбулаторного лечения. Установлен высокий уровень выявления грибов рода Candida во все периоды стационарного наблюдения (65,9±7,1%; 70,6±11,4%; 42,8±11,1%). Все 4 вида грибов рода Candida (albicans, glabrata, tropicalis, krusei) выделяли как на ранних, так и на поздних сроках стационарного лечения.

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Effectiveness of Antimicrobial Filter Placement in ICU Taps to Prevent the Occurrence of HAIs by Pseudomonas aeruginosa (12-Months)

Infection Control and Hospital Epidemiology ◽

10.1017/ice.2020.749 ◽

2020 ◽

Vol 41 (S1) ◽

pp. s206-s207

Author(s):

Pablo Chico-Sánchez ◽

Sandra Canovas-Javega ◽

Natali J. Jimenez-Sepulveda ◽

Edith Leutscher-Vasen ◽

Cesar O. Villanueva-Ruiz ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Critical Care ◽

Etiologic Agent ◽

Incidence Density ◽

Healthcare Associated Infections ◽

Open Label ◽

Filter Placement ◽

Critical Care Units ◽

Definition Of ◽

Healthcare Associated

Background:Pseudomonas aeruginosa, is the third etiologic agent of healthcare associated infections, and the most frequent pathogen in ventilator-associated pneumonia (VAP). In critical care units is associated with high mortality, long hospital stay, and high healthcare-associated costs. We evaluated the effectiveness of filter placement in the water taps in critical care units to prevent the occurrence of healthcare-associated infections (HAIa) by Pseudomonas aeruginosa. Methods: This experimental study was both cross-over and open-label in nature. We included patients admitted for >24 hours in critical care units over 24 months. The study was divided into 4 periods of 6 months each. We divided the study into 2 groups: patients in units with filters and patients in units without filters. We compared the incidence density of P. aeruginosa HAIs (number of cases divided by the number of person days) according the ECDC definition of case criteria between the groups. The 2 test was used, and the magnitude of the association was calculated as a rate ratio with a 95% confidence interval, adjusted using a Poisson regression model. Results: Overall, 1,132 patients were included in the study: 595 in units with water tap filters and 537 in units without water tap filters. HAI incidence among patients in units with water tap filters was 5.3 per 1,000 person days stay; without water tap filters, HAI incidence was 4.7 per 1,000 person days stay (HR, 0.94; 95% CI, 0.47–1.90). Conclusions: The preliminary results of this study indicate a a lower incidence of P. aeruginosa HAIs in units with filters placed in water taps than in units without filters.Disclosures: NoneFunding: None

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679. Predominance of Acinetobacter baumannii Sequence Type 2 (ST2) among Clinical Isolates from Two Detroit Hospitals using Whole Genome Sequencing

Open Forum Infectious Diseases ◽

10.1093/ofid/ofaa439.871 ◽

2020 ◽

Vol 7 (Supplement_1) ◽

pp. S392-S393

Author(s):

Piyali Chatterjee ◽

Chetan Jinadatha ◽

Keith S Kaye ◽

Hosoon Choi ◽

Yonhui Allton ◽

...

Keyword(s):

Acinetobacter Baumannii ◽

Minimum Spanning Tree ◽

Veterans Affairs ◽

Sequence Type ◽

Whole Genome ◽

Healthcare Associated Infections ◽

Department Of Veterans Affairs ◽

Healthcare Associated ◽

Sequence Types ◽

Research Grant

Abstract Background Healthcare-associated infections (HAIs) caused by Acinetobacter baumannii, an opportunistic gram-negative pathogen, often contribute to morbidity and mortality among hospitalized patients. To determine molecular epidemiology of circulating hospital Acinetobacter strains in Detroit area in a non-outbreak situation, we performed whole genome sequencing (WGS) and whole genome multilocus sequence typing (wgMLST) analysis to characterize genomic diversity. Methods We performed WGS on patient isolates from two disparate, geographically distinct tertiary care Detroit hospitals admitted to 16 intensive care units (ICU) and non-ICU wards between 2017-2019. The samples were obtained 48 hours following admission and WGS was performed using the Illumina NextSeq instrument. The contigs were de novo assembled using SPAdes and WgMLST analysis was performed using BioNumerics software v7.6. Minimum spanning tree (MST) was constructed to demonstrate the clusters in each hospital and their wards. Results WgMLST analysis was performed on a total of 62 Acinetobacter baumannii patient isolates, 33 of which were from hospital # 1 (H1) and 29 were from hospital # 2 (H2). ST2 (52%) was the predominant sequence type for both hospitals. In addition to ST2, ST93 (8%) and ST406 (8%) clusters were also found in H1. Most of the isolates were predominantly clustered in the intensive care unit (ICU) setting. Several identical sequence types of ST93 (2 identical in H1), ST36 (2 identical in H1) and ST2 (5 identical for H1 and 2 identical for H2) were also observed from different patients in both hospitals. Unlike H1, H2 only has ST2 as a predominant cluster. Other sporadic sequence types such as ST119, ST36, ST49, ST212, ST667 for H1 and ST395, ST214, ST427 and ST268 for H2 were also observed. Figure 1. MInimum Spanning Tree of Acinetobacter strains in hospitals (H1 and H2) and different wards (U1-U12). For identical strains circles are marked with dividing lines. Different sequence types (STs) are marked om the side bar. Conclusion Our data suggests that ST2 is the predominant sequence type circulating in both the geographically distinct hospitals and is endemic to the ICU. There was in hospital spread of this distinct Acinetobacter sequence type from one patient to the other, raising concerns about infection control practices and the role of environment in the spread of these infections. Early detection of these endemic strains within the hospital may be critical in preventing healthcare-associated infections in the future. Disclosures Chetan Jinadatha, MD, MPH, AHRQ (Research Grant or Support)Department of Veterans Affairs (Other Financial or Material Support, Owner: Department of Veterans Affairs. Licensed to: Xenex Disinfection System, San Antonio, TX)Inventor (Other Financial or Material Support, Methods for organizing the disinfection of one or more items contaminated with biological agents)NiH/NINR (Research Grant or Support)NSF (Research Grant or Support)Xenex Healthcare Services (Research Grant or Support) Mark Stibich, PhD MHS, Xenex Disinfection Services, Inc (Board Member, Employee)

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Diagnostic Value of Multiplex Polymerase Chain Reaction in Detection of Acinetobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia from Sepsis in Pediatrics

Recent Patents on Biotechnology ◽

10.2174/1872208315666210719104623 ◽

2021 ◽

Vol 15 ◽

Author(s):

Sara Galeb ◽

Maysaa El Sayed Zaki ◽

Raghdaa Shrief ◽

Rasha Hassan ◽

Mohamed Anies

Keyword(s):

Polymerase Chain Reaction ◽

Pseudomonas Aeruginosa ◽

Acinetobacter Baumannii ◽

Multiplex Pcr ◽

Stenotrophomonas Maltophilia ◽

Multiplex Polymerase Chain Reaction ◽

Diagnostic Value ◽

Blood Cultures ◽

Chain Reaction ◽

Polymerase Chain

Background: Proper identification of the causative organism in pediatric sepsis is crucial for early diagnosis and prevention of septic shock and organ failure. Objectives: The present study aimed to evaluate the multiplex Polymerase Chain Reaction (PCR) to detect Acinetobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia from positive blood cultures for these pathogens isolated from children, with hospital-acquired sepsis compared to the conventional biochemical reactions for identification of these organisms. Methods: This study was a cross-sectional study performed on 100 isolates from pediatric blood cultures, including Acinetobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia. The study also included 100 isolates of Escherichia coli as a negative control. All isolates were identified by API 20NE and the multiplex PCR, with primers specific to the 3 tested bacteria. Results: Multiplex PCR was positive in 96% of isolates, and 4 isolates had negative results. False positive results were reported with three E. coli strains. Multiplex PCR identified all the isolates of Acinetobacter baumannii, 29 isolates of Pseudomonas aeruginosa, and 27 isolates of Stenotrophomonas maltophilia. Compared to the biochemical identification, the diagnostic value of the multiplex PCR revealed 96.04% sensitivity, 96.9% specificity, 97.00%, positive predictive value, 96.00% negative predictive value, and 96.50% accuracy. Conclusion: The present study highlights the diagnostic value of multiplex PCR to identify Acinetobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia from positive blood cultures. Multiplex PCR was sensitive, specific, and accurate. The accuracy differs according to the organisms, with 100% accuracy for Acinetobacter baumannii.

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Prevalência de infecção da corrente sanguínea em idosos internados em um Hospital Geral

Revista Prevenção de Infecção e Saúde ◽

10.26694/repis.v1i3.4252 ◽

2015 ◽

Vol 1 (3) ◽

pp. 11

Author(s):

Marcos André Siqueira de Sousa ◽

Thays Rezende Lima ◽

Alvaro Francisco Lopes de Sousa ◽

Marcelo de Moura Carvalho ◽

Giselle Mary Ibiapina Brito ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Klebsiella Pneumoniae ◽

Acinetobacter Baumannii ◽

De Se

Objetivo: identificar a prevalência de infecção da corrente sanguínea em idosos internados em um clinica cirúrgica de um Hospital Geral. Metodologia: Trata-se de um estudo epidemiológico, retrospectivo, descritivo com abordagem quantitativa. A amostra constou de 68 pacientes internados no ano de 2013 em um Hospital de referência e ensino de Teresina-PI. Os dados foram analisados pelo SPSS Versão 10.0 e o projeto de pesquisa foi aprovado pelo Comitê de Ética em Pesquisa da Universidade Federal do Piauí (CAAE:18110614.10000.5214). Resultados: Dos 68 pacientes diagnosticados com cultura positiva pra ICS a prevalência de idosos(60 anos ou mais) foi de 49%. Houve predominância do sexo feminino (58%) e estado civil casado (43%). Os microrganismos mais prevalentes foram Acinetobacter baumannii (23,5%), Klebsiella pneumoniae (19,65), Pseudomonas aeruginosa (19,05%) e Staphylococcus coagulase-negativo(17,3%). Conclusão: A prevalência de ICS elevada revela a necessidade de se avaliar medidas de prevenção para esta faixa etária.

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СИНТЕЗ ТА ДОСЛІДЖЕННЯ АНТИМІКРОБНОЇ АКТИВНОСТІ ДЕЯКИХ ПІРАЗОЛЗАМІЩЕНИХ 7H-[1,2,4]ТРИАЗОЛО[3,4-B][1,3,4]ТІАДІАЗИНІВ

Фармацевтичний часопис ◽

10.11603/2312-0967.2021.1.11986 ◽

2021 ◽

pp. 5-13

Author(s):

I. I. Myrko ◽

T. I. Chaban ◽

V. V. Ogurtsov ◽

V. S. Matiychuk

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Candida Albicans ◽

Drug Discovery ◽

Klebsiella Pneumoniae ◽

Acinetobacter Baumannii ◽

Cryptococcus Neoformans ◽

Antimicrobial Drug

Мета роботи. Здійснити синтез деяких нових піразолзаміщених 7H-[1,2,4]триазоло[3,4-b][1,3,4]тіадіазинів та провести дослідження антимікробних властивостей синтезованих сполук. Матеріали і методи. Органічний синтез, ЯМР-спектроскопія, елементний аналіз, фармакологічний скринінг. Результати й обговорення. У результаті взаємодії eтил (2Z)-хлоро(фенілгідразоно)ацетатів з ацетилацетоном було отримано етил 4-ацетил-5-метил-1-феніл-1H-піразол-3-карбоксилати. Зазначені сполуки піддали бромуванню, що дозволило одержати цільові бромкетони. Синтезовані на даній стадії етил 1-арил-4-(бромацетил)-5-метил-1Н-піразол-3-карбоксилати було введено у взаємодію з 4-аміно-5-арил(гетарил)-2,4-дигідро-3Н-1,2,4-триазол-3-тіонами з подальшим формуванням 1,3,4-тіадіазольного циклу та отриманням відповідних етил 1-арил-4-{3-арил(гетарил)-7H-[1,2,4]триазоло[3,4-b][1,3,4]тіадіазин-6-іл)}-5-метил-1H-піразол-3-карбоксилатів. Структура синтезованих сполук підтверджена даними елементного аналізу та ЯМР спектроскопією. В рамках міжнародного проекту "The Community for Antimicrobial Drug Discovery" (CO-ADD) за підтримки Wellcome Trust (Великобританія) і університету Квінсленда (Австралія) для синтезованих сполук здійснено скринінг антимікробної активності. Як тестові мікроорганізми використовували п'ять штамів бактерій: Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603, Acinetobacter baumannii ATCC 19606, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 43300 та двох штамів грибків: Candida albicans ATCC 90028 і Cryptococcus neoformans ATCC 208821. Встановлено, що досліджувані сполуки виявляють різноманітну дію, від практично повної її відсутності до виразного антимікробного ефекту. Висновки. Здійснено синтез 12 нових етил 1-арил-4-{3-арил(гетарил)-7H-[1,2,4]триазоло[3,4-b][1,3,4]тіадіазин-6-іл)}-5-метил-1H-піразол-3-карбоксилатів. Зазначені речовини отримані шляхом взаємодії відповідних етил 1-арил-4-(бромацетил)-5-метил-1Н-піразол-3-карбоксилатів з 4-аміно-5-арил(гетарил)-2,4-дигідро-3Н-1,2,4-триазол-3-тіонами. Дослідження антимікробної активності синтезованих сполук демонструють потенціал пошуку антимікробних агентів серед зазначеного класу сполук.

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