scholarly journals Research of tomatoes Solanum lycopersicum L. originating from different climatic-geographical regions and identification of metabolites by tandem mass spectrometry

2021 ◽  
pp. 99-104
Author(s):  
O. A. Chunikhina ◽  
M. P. Razgonova ◽  
A. M. Zakharenko ◽  
K. S. Golokhvast
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Solanum Lycopersicum ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Tandem Mass ◽  
Active Compounds ◽  
Solanum Lycopersicum L ◽  
Geographical Regions ◽  
First Time

Relevance. A number of scientific studies confirm that consumption of fruits and vegetables can reduce the risk of certain chronic diseases, such as cancer and cardiovascular diseases, for example, consumption of fresh tomatoes and tomato products is inversely proportional to the development of certain types of cancer. Tomato Solanum lycopersicum L. contains a large number of polyphenolic complexes, which are biologically active compounds. In this article, the authors have attempted for the first time to present the complete metabolomic composition of Solanum lycopersicum extracts.Materials and methods: As an object of research, authors used the extracts of Solanum lycopersicum L., from the collection of the Federal Research Center N.I. Vavilov All-Russian Institute of Plant Genetic Resources, grown and collected at the Far Eastern Experiment Station Branch of the Federal State Budgetary Scientific Institution in September 2020 (varieties: k-5351 Ont77 13, Canada; k-3149 Rehovoth, Israel; 2698 Ukraine). High performance liquid chromatography (HPLC) in combination with a BRUKER DALTONIKS ion trap (tandem mass spectrometry) was used to identify target analytes in extracts obtained by the maceration method.Discussion: The results of initial studies revealed the presence of 36 biologically active compounds, of which 22 were identified for the first time in Solanum lycopersicum L. These are Apigenin, Luteolin, Kaempferol, Taxifolin, Myricetin, Coutaric acid, Caffeoylmalic acid, Caftaric acid, Dicaffeoylquinic acid, coumarins Fraxetin, and Fraxetin-7-O-beta-glucuronide, Pelargonidin, Salvianolic acid D, Rosmanol, Colnelenic acid, Ethyl rosemary, lignan Medioresinol-O-hexoside, Squalene, etc. The findings will help to intensify future research into the development and production of various functional food products containing targeted extracts of Solanum lycopersicum L.

scholarly journals Characterization by Tandem Mass Spectrometry of Biologically Active Compounds Produced by Bacillus Strains

2015 ◽  
Vol 1 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Armelle Tosco ◽  
Anne Chobelet ◽  
Katell Bathany ◽  
Jean-Marie Schmitter ◽  
Maria C. Urdaci ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Tandem Mass ◽  
Active Compounds ◽  
Bacillus Strains

Biomimetic nanofiber by electrospinning of snail mucus

2020 ◽  
Vol 12 ◽  
Author(s):  
Congcong Zhu ◽  
Tianyi Zhong ◽  
Baoqi Zuo
Keyword(s):  
Crucial Role ◽  
Natural Fiber ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Fibrous Structure ◽  
Dissolution Time ◽  
Active Compounds ◽  
Different Diameters ◽  
First Time ◽  
Biomimetic Nanofibers

Background: Snail mucus (SM) is secreted by the pedal gland of snails, and has a fibrous structure when it crawls upside down on the plane. It contains biologically active compounds that have medical functions, such as glycol acid, natural antibiotics, and glycoprotein. Methods: For this paper, we prepared fibers using electrospinning to simulate this natural fiber for the first time, and we can produce the nanofiber with mucus from a snail. The effects of dissolution time and the spun solution were also investigated. Result: The results show that biomimetic nanofibers with different diameters can be obtained using electrospinning. When the concentration of the spun liquid was increased from 6wt% to 8wt%, a fiber with about a 200 nm diameter can be obtained. Adjusting the concentration plays a crucial role in electrospinning.

Development of a method for multiple vitamin D metabolite measurements by liquid chromatography coupled with tandem mass spectrometry in dried blood spots

The Analyst ◽  
2019 ◽  
Vol 144 (1) ◽  
pp. 299-309 ◽  
Author(s):  
R. Rola ◽  
K. Kowalski ◽  
T. Bieńkowski ◽  
A. Kołodyńska-Goworek ◽  
S. Studzińska
Keyword(s):  
Mass Spectrometry ◽  
Vitamin D ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Dried Blood Spots ◽  
Tandem Mass ◽  
Vitamin D Metabolites ◽  
Blood Spots ◽  
First Time

Profiling of vitamin D metabolites in dried blood spots, including 24,25(OH)2D3, has been performed for the first time.

scholarly journals Antimicrobial Activities of Different Fractions from Mucus of the Garden Snail Cornu aspersum

Biomedicines ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 315 ◽  
Author(s):  
Aleksandar Dolashki ◽  
Lyudmila Velkova ◽  
Elmira Daskalova ◽  
N. Zheleva ◽  
Yana Topalova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Antibacterial Activity ◽  
Tandem Mass Spectrometry ◽  
De Novo ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
New Drugs ◽  
Tandem Mass ◽  
Bacterial Strains ◽  
Cornu Aspersum

Natural products have long played a major role in medicine and science. The garden snail Cornu aspersum is a rich source of biologically active natural substances that might be an important source for new drugs to treat human disease. Based on our previous studies, nine fractions containing compounds with Mw <3 kDa; <10 kDa; <20 kDa; >20 kDa; >30 kDa>50 kDa and between 3 and 5 kDa; 5 and 10 kDa; and 10 and 30 kDa were purified from the mucus of C. aspersum and analyzed by tandem mass spectrometry (MALDI-TOF/TOF). Seventeen novel peptides with potential antibacterial activity were identified by de novo MS/MS sequencing using tandem mass spectrometry. The different fractions were tested for antibacterial activity against Gram─ (Pseudomonas aureofaciens and Escherichia coli) and Gram+ (Brevibacillus laterosporus) bacterial strains as well the anaerobic bacterium Clostridium perfringens. These results revealed that the peptide fractions exhibit a predominant antibacterial activity against B. laterosporus; the fraction with Mw 10–30 kDa against E. coli; another peptide fraction <20 kDa against P. aureofaciens; and the protein fraction >20 kDa against the bacterial strain C. perfringens. The discovery of new antimicrobial peptides (AMPs) from natural sources is of great importance for public health due to the AMPs’ effective antimicrobial activities and low resistance rates.

scholarly journals First Detection of Tetrodotoxins in the Cotylean Flatworm Prosthiostomum trilineatum

Marine Drugs ◽  
2021 ◽  
Vol 19 (1) ◽  
pp. 40
Author(s):  
Rei Suo ◽  
Maho Kashitani ◽  
Hikaru Oyama ◽  
Masaatsu Adachi ◽  
Ryota Nakahigashi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
High Performance ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
High Concentrations ◽  
First Time

Several polyclad flatworm species are known to contain high levels of tetrodotoxin (TTX), but currently TTX-bearing flatworms seem to be restricted to specific Planocera lineages belonging to the suborder Acotylea. During our ongoing study of flatworm toxins, high concentrations of TTXs were detected for the first time in the flatworm Prosthiostomum trilineatum, suborder Cotylea, from the coastal area of Hayama, Kanagawa, Japan. Toxin levels were investigated by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS), revealing that this species contains comparable concentrations of toxins as seen in planocerid flatworms such as Planocera multitentaculata. This finding indicated that there may be other species with significant levels of TTXs. The distribution of TTXs among other flatworm species is thus of great interest.

Sign in / Sign up

Export Citation Format

Share Document