​Sex Ratio of Calves Resulted from Artificial Insemination Implementation using Sexed Semen with Percoll Gradient Density Centrifugation Method in Ongole Crossbred Cows

2021 ◽  
Author(s):  
T. Susilawati ◽  
W.O. Bustari ◽  
I.P.B. Crisara ◽  
Kuswati . ◽  
A.N. Huda ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Meat Consumption ◽  
Double Dose ◽  
Percoll Gradient ◽  
Crossbred Cows ◽  
Density Centrifugation ◽  
Gradient Density ◽  
Sexed Semen ◽  
Centrifugation Method

Background: Productivity of existing cattles in Indonesia is necessarily to be increase to balance meat consumption in this country. Determination of offspring of certain sex can be obtained from Percoll gradient density centrifugation.The purpose of this research was to elucidate the proportion of male calves that can result from artificial insemination using single and double doses of sexed semen in Ongole crossbred cows. Methods: The sexed semen samples were obtained through Percoll gradient density centrifugation performed by the Artificial Insemination Center. The artificial insemination method adopted here was deep insemination. As much as 10 ml of BIO ATP® (Rheinvet) was injected in each cow before immediately insemination. Further, as much as 3 kg/day of additional feed was given over three days after insemination, with a protein level of about 12%. Result: Our results showed the proportion of Y-bearing sperm among non-sexed semen was 52.77% and among sexed semen, was 80.79%. Further, 54.17% of the non-sexed semen, 42.11% of the single-dose sexed semen and 78.95% of the double-dose sexed semen treatments yielded male calves.

scholarly journals Tingkat Keberhasilan Inseminasi Buatan Menggunakan Semen Beku Sexing pada Bangsa Sapi yang Berbeda

2020 ◽  
Vol 20 (1) ◽  
pp. 17-21
Author(s):  
Wiranto Wiranto ◽  
Kuswati Kuswati ◽  
Rizki Prafitri ◽  
Asri Nurul Huda ◽  
Aulia Puspita Anugra Yekti ◽  
...  
Keyword(s):  
Success Rate ◽  
Artificial Insemination ◽  
Return Rate ◽  
Conception Rate ◽  
Double Dose ◽  
Percoll Gradient ◽  
Deposition Technique ◽  
Crossbred Cattle ◽  
Frozen Semen ◽  
Gradient Density

ABSTRAK. Penelitian ini bertujuan mengevaluasi tingkat keberhasilan IB semen beku sexing Y. Penelitian menggunakan 39 sapi Peranakan Ongole dan 77 sapi Persilangan Limousin yang di IB secara double dosis. IB menggunakan semen beku spermatozoa Y dengan metode pemisahan Sentrifugasi Gradien Densitas Percoll (SGDP) sapi Limousin yang diproduksi oleh Balai Besar Inseminasi Buatan (BBIB) Singosari. Teknik deposisi semen adalah 4+ yaitu pada posisi cornua uteri selanjutnya dilakukan IB pada jam ke 2 dan ke 8. Untuk akseptor dilakukan penyuntikan BioATP+ dan pemberian pakan konsentrat 3 kg per hari selama 3 hari setelah IB. Variabel penelitian meliputi Non Return Rate (NRR), Service Per Conception (S/C), dan Conception Rate (CR). Hasil penelitian menunjukkan bahwa IB sapi Peranakan Ongole dan sapi Persilangan Limousin yaitu NRR1 sebesar 82,05% dan 89,61%, nilai NRR2 sebesar 76,92% dan 84,42%, nilai CR sebesar 58,97% dan 74,03%, serta nilai S/C sebesar 1,78% dan 1,46%. Kesimpulan penelitian adalah IB menggunakan semen beku hasil sexing Y sapi Limousin pada sapi persilangan Limousin memiliki tingkat keberhasilan yang lebih tinggi dibandingkan sapi Peranakan Ongole.  (The success rate of artificial insemination using frozen sexing semen of different breed cow) ABSTRACT. This study aimed to evaluate the success rate of AI frozen semen sexing Y. The material in this study used 39 Filial Ongole and 77 Limousin crossbred cattle in AI in double dose. The spermatozoa used were Y frozen semen of Limousin bull with the Percoll Gradient Density Centrifugation (SGDP) separation method produced by the Singosari Center for Artificial Insemination. The semen deposition technique was 4+, which has the position of the cornua uteri (deep Insemination), then AI implemented with a double dose at the 2nd and 8th hours after estrous. The cow acceptor was injected with BioATP+ and feed by 3 kg concentrate per day for three days after AI. Research variables include Non-Return Rate (NRR), Service Per Conception (S/C), and Conception Rate (CR). The results showed that AI of Peranakan Ongol and Limousin crossbred cattle on NRR1, NRR2, CR values, and S/C values respectively was 82.05% and 89.61%; 76.92% and 84.42%; 58.97% and 74.03%; 1.78% and 1.46%. In conclusion, AI using frozen semen from sexing Y Limousin cattle in Limousin crossbred cows had a higher success rate than Filial Ongole cattle.

scholarly journals Pemisahan Spermatozoa Berkromosom X dan Y Kambing Boer dan Aplikasinya Melalui Inseminasi Buatan Untuk Mendapatkan Jenis Kelamin Anak Sesuai Harapan

2013 ◽  
Vol 13 (1) ◽  
pp. 6-15
Author(s):  
Dasrul Dasrul ◽  
M. Aman Yaman ◽  
Zulfan Zulfan
Keyword(s):  
Sex Ratio ◽  
Conception Rate ◽  
Separation Control ◽  
Percoll Gradient ◽  
Boer Goat ◽  
Density Centrifugation ◽  
Group Treatments ◽  
Gradient Density ◽  
And Control ◽  
Fresh Semen

Separation of spermatozoa with x and y chromosome at boer goat and its application by artificial insemination for kid sex purposeABSTRACT. The purposes of this experiment are to investigate the separation of X and Y spermatozoa by measuring the spermatozoa quality, sex ratio between X and Y, capacity of fertility indicated by conception rate and sex ratio of goat boar kids. Samples, used in this experiment, are fresh semen from Boer goat with high quality consists of 4 group treatments with 6 replications 1) group of spermatozoa without separation (control), 2) group of spermatozoa separated by percoll gradient density centrifugation 3 levels (P1), 5 levels (P2) and swine up (P3). The observed parameters are spermatozoa quality, X and Y spermatozoa ratio, fertility’s capacity and sex ratio on the birth. Quality examination of spermatozoa and identify X and Y spermatozoa is based on the standard method of WHO. The conception rate was based on the ratio of pregnant goat after the first insemination. Data of spermatozoa quality and spermatozoa ratio were analyzed by using analisis of variance (ANOVA) and further analysis by LSD if there were differences between treatments. The results of this experiment showed that spermatozoa quality Boer goat significantly reduced (p0,05) after separation with percoll gradient density centrifugation  and swim up. Percentage spermatozoa X after percoll gradient density centrifugation was significantly higher (P0,05) compared to control and swim up. Meanwhile, the Y spermatozoa population was significantly higher (P0,05) after swim up treatment compared to percoll gradient density centrifugation and control. The percentage of sex ratio (male: female) after insemination from percoll gradient density centrifugation produced more female than male. On the other hand, insemination from swim up produced more male than female. Sex ratio produced from separation of percoll gradient density centrifugation, swim up was difference from control semen (P0,05). From this experiment, it was concluded that spermatozoa separation by percoll gradient density centrifugation and swim up can be used as one of the methods to separate   X and Y spermatozoa and further can be applied to get preferred sex animals.

The use of sexed semen in dairy herds

Livestock ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 282-286
Author(s):  
Mark Burnell
Keyword(s):  
Flow Cytometry ◽  
Artificial Insemination ◽  
Genetic Improvement ◽  
Dairy Herd ◽  
Dairy Herds ◽  
Sexed Semen ◽  
Sorting Process ◽  
Sperm Sorting ◽  
The Uk

Artificial insemination of cattle became a commercial reality in 1936, and in 1952 the first calves were born as a result of freeze-thawed semen insemination. The long-held aspiration for pre determination of sex became a reality with the development of sperm sorting technology with the first female calves being born in the UK in 1999. While most breeding companies supply sexed semen sorted by the original flow cytometry method, Genus ABS have developed the method of sperm deactivation. Whatever the method sexing accuracy exceeds 90% and conception rates can be expected to be comparable to conventional semen but only when management is good and importantly there are considerable variations in the fertility of different bulls as a result of the sorting process. The use of sexed semen offers producers an opportunity to maximise the beef production from the dairy herd while accelerating genetic improvement.

scholarly journals Economic mechanism of the region breeding service activity

2021 ◽  
Vol 285 ◽  
pp. 01011
Author(s):  
Konstantin Titorenko ◽  
Kirill Zhichkin
Keyword(s):  
Artificial Insemination ◽  
Cattle Breeding ◽  
Economic Mechanism ◽  
Agricultural Enterprises ◽  
Genetic Potential ◽  
Sexed Semen ◽  
Service Activity ◽  
Main Factors ◽  
Cattle Reproduction ◽  
Donor Cows

The article examines the concept and elements of the economic mechanism of breeding in the region. The main goal of this work is to determine the features of the economic mechanism of the breeding service in the region for its further optimization. At the same time, the following tasks are being solved: - to reveal the features of the economic mechanism of the breeding service of the region; - to determine the main factors influencing the activities of the breeding service in the region; - to analyze the effectiveness of state support for the breeding service of the region. We note that the technologies of cattle reproduction using the method of artificial insemination with sexed semen and the method of embryo transfer in agricultural enterprises in practice have proven their economic efficiency and high profitability indicators. But to obtain the described results, the source material (sexed seed and embryo), as well as the qualities of the donor and recipient, as well as systematic work in the industry of dairy and beef cattle breeding in the Samara region, are extremely important. Raised cows with high genetic potential, obtained from artificial insemination with sexed semen and embryo transplantation, can then be used as donor cows to obtain embryos and their further transplantation in other agricultural enterprises.

scholarly journals ELECTRON MICROSCOPIC EVIDENCE FOR PURIFICATION OF COCONUT ROOT (WILT) DISEASE PHYTOPLASMA

CORD ◽  
2002 ◽  
Vol 18 (01) ◽  
pp. 34
Author(s):  
M. Mayilvaganan ◽  
J. J. Solomon
Keyword(s):  
Plant Material ◽  
Gradient Centrifugation ◽  
Spherical Shape ◽  
Electron Microscopic Examination ◽  
Wilt Disease ◽  
Diseased Plant ◽  
Percoll Gradient ◽  
Electron Microscopic ◽  
Centrifugation Method ◽  
Discontinuous Percoll Gradient

Root (wilt) disease phytoplasma was purified from diseased coconut tissues using discontinuous Percoll gradient centrifugation method. Crude sap prepared from coconut was layered on a discontinuous Percoll gradient of 15, 30, 50 and 60%(v/v). After centrifugation at 20,000 g for 30 min, the turbid fraction formed on the top of 30% gradient in the diseased plant material was recovered, processed and fixed for electron microscopy. Electron microscopic examination of sections prepared from purified preparation of diseased plant material showed typical cells of root (wilt) phytoplasma with heterogeneous sizes and more or less spherical shape that are similar to those found in sieve elements of diseased tissues and salivary glands of infective (viruliforms) insect vectors. These purified phytoplasma bodies showed trilaminar membrane with internal materials of ribosome granules and DNA fibrils. However, the yield in terms of number of cells was fewer and in addition to intact bodies, free membranes and empty bodies lacking internal contents also were observed.

Artificial insemination of heifers with cooled, unfrozen sexed semen

1998 ◽  
Vol 49 (1) ◽  
pp. 365 ◽  
Author(s):  
G.E. Seidel ◽  
L.A. Herickhoff ◽  
J.L. Schenk ◽  
S.P. Doyle ◽  
R.D. Green
Keyword(s):  
Artificial Insemination ◽  
Sexed Semen

A cerebral decarboxylase for 5-hydroxytryptophane in humans

1961 ◽  
Vol 16 (6) ◽  
pp. 1050-1054 ◽  
Author(s):  
William Sacks
Keyword(s):  
Intravenous Injection ◽  
Normal Control ◽  
Cerebral Metabolism ◽  
Mental Patient ◽  
Double Dose ◽  
Control Subjects ◽  
Arterial Blood ◽  
Body Tissues

Little or no cerebral decarboxylation of 5-hydroxytryptophane could be found using the in vivo technique developed in this laboratory for determination of human cerebral metabolism. Following intravenous injection of dl-5-hydroxytryptophanecarboxyl-C14 little or no significant venous-arterial C14O2 differences resulted in four normal control subjects and four chronic mental patients. No significant differences were found between the two groups. Levels of arterial blood C14O2 activities showed that 5-hydroxytryptophane was decarboxylated readily by other body tissues. Of four subjects pretreated with 1-benzyl-2-methyl-5-methoxytryptamine (BAS), slightly lowered results occurred with the mental patient pretreated with a double dose of BAS. Submitted on June 19, 1961

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