scholarly journals Tingkat Keberhasilan Inseminasi Buatan Menggunakan Semen Beku Sexing pada Bangsa Sapi yang Berbeda

2020 ◽  
Vol 20 (1) ◽  
pp. 17-21
Author(s):  
Wiranto Wiranto ◽  
Kuswati Kuswati ◽  
Rizki Prafitri ◽  
Asri Nurul Huda ◽  
Aulia Puspita Anugra Yekti ◽  
...  

ABSTRAK. Penelitian ini bertujuan mengevaluasi tingkat keberhasilan IB semen beku sexing Y. Penelitian menggunakan 39 sapi Peranakan Ongole dan 77 sapi Persilangan Limousin yang di IB secara double dosis. IB menggunakan semen beku spermatozoa Y dengan metode pemisahan Sentrifugasi Gradien Densitas Percoll (SGDP) sapi Limousin yang diproduksi oleh Balai Besar Inseminasi Buatan (BBIB) Singosari. Teknik deposisi semen adalah 4+ yaitu pada posisi cornua uteri selanjutnya dilakukan IB pada jam ke 2 dan ke 8. Untuk akseptor dilakukan penyuntikan BioATP+ dan pemberian pakan konsentrat 3 kg per hari selama 3 hari setelah IB. Variabel penelitian meliputi Non Return Rate (NRR), Service Per Conception (S/C), dan Conception Rate (CR). Hasil penelitian menunjukkan bahwa IB sapi Peranakan Ongole dan sapi Persilangan Limousin yaitu NRR1 sebesar 82,05% dan 89,61%, nilai NRR2 sebesar 76,92% dan 84,42%, nilai CR sebesar 58,97% dan 74,03%, serta nilai S/C sebesar 1,78% dan 1,46%. Kesimpulan penelitian adalah IB menggunakan semen beku hasil sexing Y sapi Limousin pada sapi persilangan Limousin memiliki tingkat keberhasilan yang lebih tinggi dibandingkan sapi Peranakan Ongole.  (The success rate of artificial insemination using frozen sexing semen of different breed cow) ABSTRACT. This study aimed to evaluate the success rate of AI frozen semen sexing Y. The material in this study used 39 Filial Ongole and 77 Limousin crossbred cattle in AI in double dose. The spermatozoa used were Y frozen semen of Limousin bull with the Percoll Gradient Density Centrifugation (SGDP) separation method produced by the Singosari Center for Artificial Insemination. The semen deposition technique was 4+, which has the position of the cornua uteri (deep Insemination), then AI implemented with a double dose at the 2nd and 8th hours after estrous. The cow acceptor was injected with BioATP+ and feed by 3 kg concentrate per day for three days after AI. Research variables include Non-Return Rate (NRR), Service Per Conception (S/C), and Conception Rate (CR). The results showed that AI of Peranakan Ongol and Limousin crossbred cattle on NRR1, NRR2, CR values, and S/C values respectively was 82.05% and 89.61%; 76.92% and 84.42%; 58.97% and 74.03%; 1.78% and 1.46%. In conclusion, AI using frozen semen from sexing Y Limousin cattle in Limousin crossbred cows had a higher success rate than Filial Ongole cattle.

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Mohamad Nur Amin

This study aims to determine the role of artificial insemination (IB) on the marriage system in the group of caromah cattle farmers in Taluditi Sub-District, Pohuwato Regency. The livestock population of 60 cows consisting of A 32 cages and enclosure B 28 tails. The analysis used was descriptive analysis to get the percentage and average number of pregnancies. Parameters observed include: Service Per Conception (S / C) and Conception Rate (C / R). The results showed that (Service Per Conception) obtained results at enclosure A, the S / C value was 1.7 times, and at enclosure B the S / C value was 1.7 times. The percentage of pregnancy (Conception Rate) is obtained at A cage 79% and the result in cage B is C / R value of 67%. The results obtained are strongly influenced by 4 factors, namely; namely frozen semen, female cattle as IB acceptors, inseminator skills and zooteknis knowledge of farmers. The conclusion of this study is that the success rate of Artificial Insemination in Taluditi District, Pohuwato Regency can be categorized as good.


2018 ◽  
Vol 28 (3) ◽  
pp. 259 ◽  
Author(s):  
Dwi Susan Setiyani ◽  
Aulia Puspita Anugra Yekti ◽  
Kuswati Kuswati ◽  
Trinil Susilawati

<p>The Artificial Insemination (AI)  results in Beef cattle are expected to be male calf because they can increaseweight gain rapidly, so in this study AI was carried out by using sexing semen with Percoll density gradient centrifugation method containing Y sperm.This study was conducted to evaluate the success rate of AI using frozen sexing semen on Ongole Cross Breed. The study method used wasexperimental, comparing AI frozen sexing semen and frozen semen in 54 Ongole Cross Breed. The study variableswere Non Return Rate<sub>1</sub> (NRR<sub>1</sub>), Non Return Rate<sub>2</sub> (NRR<sub>2</sub>) and Conception Rate (CR).The study showed that the success rate of based on the value of NRR<sub>1</sub> and NRR<sub>2</sub>AI using frozen sexing semen on Ongole Cross Breed is lower than un sexed frozen semen with the NRR<sub>1</sub> value is 73,91%; 93,54% while the value of NRR<sub>2</sub> was 69,56%; 83,87%. Based on CR value of frozen sexing semen higher than un sexed frozen semen showed CR valuewas 43,47%; 35,48%.</p>


Author(s):  
T. Susilawati ◽  
W.O. Bustari ◽  
I.P.B. Crisara ◽  
Kuswati . ◽  
A.N. Huda ◽  
...  

Background: Productivity of existing cattles in Indonesia is necessarily to be increase to balance meat consumption in this country. Determination of offspring of certain sex can be obtained from Percoll gradient density centrifugation.The purpose of this research was to elucidate the proportion of male calves that can result from artificial insemination using single and double doses of sexed semen in Ongole crossbred cows. Methods: The sexed semen samples were obtained through Percoll gradient density centrifugation performed by the Artificial Insemination Center. The artificial insemination method adopted here was deep insemination. As much as 10 ml of BIO ATP® (Rheinvet) was injected in each cow before immediately insemination. Further, as much as 3 kg/day of additional feed was given over three days after insemination, with a protein level of about 12%. Result: Our results showed the proportion of Y-bearing sperm among non-sexed semen was 52.77% and among sexed semen, was 80.79%. Further, 54.17% of the non-sexed semen, 42.11% of the single-dose sexed semen and 78.95% of the double-dose sexed semen treatments yielded male calves.


2017 ◽  
Vol 18 (3) ◽  
pp. 353 ◽  
Author(s):  
Takdir Saili ◽  
La Ode Nafiu ◽  
La Ode Baa ◽  
Syam Rahadi ◽  
Astriana Napirah ◽  
...  

Estrus synchronization is one of the reproduction technology applied in the cows that aim to induce estrus of some cows to occur in the same time. In this research, all cows expressing estrus would be inseminated using sexed sperm that produced using column albumen method. Sexing sperm technology could be applied to produce the desired sex of calf. Effectivity of chilled sexed sperm to produce the desired sex of calf was evaluated in this research. Sixty three bali cows divided into 2 groups of ages (3-4 yo. and 5- 6 yo.) were used and performed synchronization using Capriglandin (PGF2a) hormone prior to application of artificial insemination with chilled sexed sperm. Variable measured were success rate of synchronization, estrus post synchronization, estrus quality, non return rate, conception rate and calving rate. The results showed that 62.90% of cows showed estrus following synchronization, estrus post synchronization occurred at 71.73 hours following synchronization, and estrus quality was 2.5%. There were 82.54% of inseminated cows was predicted to be pregnant after first insemination using chilled sexed sperm. However, only 73.02% could maintain the pregnancy up to calving. Whereas 78.26 % of newborn calf was male calf. Finally, it was concluded that PGF2a was effective to trigger estrus in bali cows, while sexed sperm still had good fertility and the sex of newborn calf was 78,26% confirmed the prediction. ABSTRAK Sinkronisasi estrus merupakan salah satu teknologi reproduksi yang diterapkan pada ternak sapi betina dengan tujuan untuk mendapatkan sejumlah ternak yang estrus secara bersamaan. Pada penelitian ini ternak yang mengalami estrus tersebut diinseminasi menggunakan spermatozoa yang telah melalui proses sexing menggunakan metode kolum albumen. Teknologi sexing spermatozoa memungkinkan untuk mengatur kelahiran anak ternak sesuai jenis kelamin yang diinginkan. Penelitian ini dilakukan untuk mengevaluasi efektivitas penggunaan semen cair hasil sexing dalam memproduksi anak sapi dengan jenis kelamin yang diinginkan. Sapi bali induk sebanyak 63 ekor yang dibagi ke dalam dua kelompok, umur 3-4 tahun dan 5-6 tahun digunakan sebagai akseptor pada penelitian ini. Sebelum inseminasi buatan (IB) dilakukan, semua sapi akseptor disinkronisasi menggunakan hormon Capriglandin (PGF2a). Variabel yang diamati adalah keberhasilan sinkronisasi, estrus pascapenyerentakan birahi, kualitas estrus, non return rate, conception rate dan calving rate. Hasil penelitian menunjukkan bahwa 62,90% sapi mengalami estrus setelah sinkronisasi dengan rataan waktu munculnya estrus 71,73 jam dan kualitas estrus 2,5. Sapi yang diprediksi bunting setelah inseminasi pertama dengan semen hasil sexing mencapai 82,54%. Jumlah sapi yang mampu mempertahankan kebuntingan hingga melahirkan hanya 73,02% dengan persentase jumlah anak sapi jantan yang dilahirkan mencapai 78,26%. Simpulan yang dapat diperoleh dari hasil penelitian ini adalah PGF2a cukup efektif merangsang munculnya estrus pada sapi bali induk dan spermatozoa hasil sexing masih mempunyai daya fertilitas yang cukup baik dengan tingkat kesesuaian jenis kelamin anak sapi yang dilahirkan mencapai 78,26%.


Author(s):  
Robi Agustinadi Tati Rohayati, dan Asep Permadi Gumelar2

Abstrak Penelitian mengenai Evaluasi Tingkat Keberhasilan Sapi Potong Betina di Kecamatan Selaawi Kabupaten Garut telah dilaksanakan pada tanggal 1 Juli sampai dengan tanggal 31 Juli 2015.Penelitian bertujuan untuk mengetahui tingkat keberhasilan inseminasi buatan yang meliputi service per conception (S/C), conception rate (CR) dan Calving Rate (C/R) pada sapi potong di Kecamatan Selaawi Kabupaten Garut. Metode penelitian yang digunakan adalah metode deskriptif dengan teknik studi kasus. Peubah yang diamati terdiri dari service per conception (S/C), conception rate (CR) dan Calving Rate (C/R). Hasil penelitian menunjukan bahwa rataan service per conception (S/C) adalah sebesar 1,4, Conception Rate (CR) sebesar 71%, Calving Rate (C/R) 83%. Dengan demikian tingkat keberhasilan inseminasi buatan pada sapi potong betina di Kecamatan Selaawi sudah baik. Kata kunci: Inseminasi Buatan, S/C, CR, C/R, sapi potong Abstract Research on Evaluation of Beef Cattle Females Success Rate in District Selaawi Garut was held on July 1 until the date of July 31, 2015. The study aims to determine the success rate of artificial insemination which includes service per conception (S/C), conception rate (CR) and Calving Rate (C/R) in beef cattle in the district of Garut Selaawi. The method used is descriptive method with a case study. Variables observed consisted of service per conception (S/C), conception rate (CR) and Calving Rate (C/R). The results showed that the average services per conception (S/C) is at 1.4, Conception Rate (CR) of 71 % , Calving Rate ( C / R ) 83 %. Thus the success rate of artificial insemination in beef cattle females in Sub Selaawi is good. Keywords: artificial insemination, service per conception, conception rate, Calving Rate, beef cattle


2018 ◽  
Vol 28 (3) ◽  
pp. 241 ◽  
Author(s):  
Aulia Puspita Anugra Yekti ◽  
Titah Ulya Kurniaesa ◽  
Nurul Isnaini ◽  
Kuswati Kuswati ◽  
Trinil Susilawati

<p>Artificial Insemination (AI) is a technique that is widely used by farmers to improve the genetic quality of livestock. This method can be increased in value by using sexing semen that can regulate and produce the sex of the offspring as expected. The purpose of this study was to determine the success of AI by using frozen sexing semen on Ongole Crossbred cows in Malang Regency. The materials used were 98 Ongole crossbred cows in traditional farm of Malang Regency especially in Pakis, Tumpang, Jabung and Singosari Districts. The method used was a field experiment with two treatments: 49 cows were inseminated by using frozen sexing semen (P1) and 49 cows were inseminated by using non sexing semen (P0). The variables observed were the Non-Return Rate (NRR) value observedin 3 estrous cycle after AI and CR value based on pregnancy observation at 8 months of pregnancy. Data were analyzed descriptively and continued with a one-proportion z-test. The datashowed that the CR value on P1; P0 were 55.10% and 65, 31% respectively.furthemore, the NRR value on P1 from NRR<sub>1</sub> to NRR<sub>3 </sub>were 87,76%, 73,47% and 67,35%, while on P0 95,92%, 83,67% and 75,51% respectively.It can be conclude thatCR and NRR value of sexing semenwas lower than non sexing semen, however sexing semen can be applied for certain purposes such as producing offspring as expected</p>


Author(s):  
Duta Setiawan

This study aims to Determine the success rate of the Artificial Insemination in North Kayong Regency of West Kalimantan Province roomates has been done by IB officers. Artificial insemination is the process of introduction or delivery of cement into the genitals of female cows by means of made devices. North Kayong districts Regency is one of the which is administratively established Republic of Indonesia based on Law no. 6 of 2007 is an area that is developing cattle. The location that Became the focus of the evaluation of the success of Artificial Insemination is in three districts of Maya Island district, Sukadana and Seponti. Data used in this research is secondary Data Obtained from inseminator. The parameters used in this evaluation are the Non Return Rate (NRR), Service per Conception (S / C) and Calving Rate (CvR). The results Showed that the average of the three sub-districts in North Kayong district was for the highest 90% NRR in Sukadana district and the Lowest score of 67% in the Mayan Island district. S / C Obtained the highest number of 3.4 in Maya Island district and the Lowest S / C number district of Seponti 1.8. The highest score was 92% CvR in Sukadana district and the cancel CvR score was 64% in the Mayan Island district. The Conclusions Obtained based on the value of NRR, S / C, and CvR on artificial insemination evaluation with the best artificial insemination implementation are Sukadana district, followed by district of Seponti and last is Maya Island district.


1990 ◽  
Vol 50 (1) ◽  
pp. 41-49 ◽  
Author(s):  
M. Garcia ◽  
W. Huanca ◽  
L. Echevarria

ABSTRACTBreeding data from 1543 artificial inseminations, performed on 763 purebred and crossbred zebu cattle reared on 73 small farms, in the Amazon basin of Peru from 1980 to 1986, were evaluated. Inseminations were performed during morning hours using Holstein or Brown Swiss frozen semen. The calving to first oestrus and calving to conception intervals, number of services per conception, intervals between services and conception rate were evaluated. A total of 64-4% of first services and 61-4% of conceptions occurred between the middle of the dry season and the middle of the rainy season. Farm effect proved to be the most important source of variation for all reproductive traits. Other important factors influencing the interval from parturition to first service were breed of cow, parity and season of service. Parity influenced the interval from parturition to conception while parity, time of oestrous detection and inseminator influenced conception rate. The long post-partum anoestrous interval was found to be the most important factor limiting reproductive efficiency on small farms involved in the artificial insemination programme. The reproductive performance of the crossbred cattle tended to be better than that of the purebred zebu cattle.


2019 ◽  
Vol 3 (2) ◽  
pp. 47
Author(s):  
Sadjadi Sadjadi ◽  
Ririn Novita ◽  
Eko Triawan

he purpose of this study was to see the success rate of Artificial Insemination on beef cattle  in Purwodadi district, Musi Rawas Regency. The parameters observed is Service per Conception (S/C), Conception Rate (CR), Non Raturn Rate (NRR) and Calving Interval (CI).  Survey method isused  in this study by taking all area of Purwodadi district as the observed area because of its livestock population then analysed by using Slovin Formula to obtain 93 samples. The results of this study concluded that the Conseption Rate (C/R) in Purwodadi District was very good because it was obtained 70.96%. Figures for   S/C in Purwodadi sub-district showed an average of 1.4. As for the NRR value in Purwodadi Subdistrict, NRR(0-30) obtained a percentage of 70.96%. NRR(31-60) obtained 93.54% and NRR(61-90) obtained 100%. The value of the Calving Interval (CI) is very good, reaching an average of 14.7 or 14 months 7 days.Keywords: Artificial insemination, Purwodadi district, Beef Cattle, Success Rates


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