Major histocompatibility complex (DRB3) geneexpression pattern indicates differences in BrucellaabortusS19 vaccine induced immune responsein Karan Fries and Sahiwal cattle

2018 ◽  
Author(s):  
D. Ravi Kumar ◽  
S. K. Mishra ◽  
Anshuman Kumar ◽  
Vineeth M.R. S. Jayakumar ◽  
A. Sakthivel Selvan ◽  
...  
Keyword(s):  
Genetic Basis ◽  
Mononuclear Cells ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Strong Response ◽  
Peripheral Blood Mononuclear ◽  
Genetic Groups ◽  
Histocompatibility Complex ◽  
Antigenic Reactivity ◽  
Karan Fries

Brucella abortus S19 strain vaccination is most effectively used as a tool to control the brucellosis in cattle. To understand the genetic basis of differences in immune responsiveness after immunization in cattle of different genotypes, we assessedthe expression of MHC-DRB3 antigen receptor molecule in six each female calves of Karan Fries crossbreds (KF, Bos indicus x Bos taurus) and Sahiwal (Bos indicus) vaccinated with Brucella S19. Serum and peripheral blood mononuclear cells (PBMC) were isolated from blood collected on 0(before vaccination) and 7, 14 and 28 day of vaccination. Antigenic response was assessed for these days in both the groups using Rose Bengal Plate form Test (RBPT). At 0d, the calves of both groups showed no antigen agglutination, confirming the calves free from the infection.The serum of 7d onward started showing the agglutination with more strong response in later stages specifically in KF, indicating increased immune response against Brucella. Therefore, RBPT can be used as earliest screening (7d onward) for Brucella antigenic reactivity in both cattle groups. The expression of DRB3 gene started with slight upregulation after vaccination,in general, however without any significant differences between two different genetic groups upto14d.The significant (p Lass Than 0.01) higher expression (8 times) of DRB3was observed in KF than Sahiwal at 28d. The study indicated that antigenic reactivity and MHC-DRB3 expression elicited by Brucella S19 vaccination was more prominent in KFduring initial days, which may provide an extra advantage to the host for antigen binding, thereby better immune protection at later stage.

Molecular characterization and SNP identification in HSPB6 gene in Karan Fries (Bos taurus x Bos indicus) cattle

2017 ◽  
Vol 49 (5) ◽  
pp. 1059-1063
Author(s):  
Rakesh Kumar ◽  
I. D. Gupta ◽  
Archana Verma ◽  
Ragini Kumari ◽  
Nishant Verma
Keyword(s):  
Molecular Characterization ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Karan Fries

Identification of alloreactive T-cell epitopes on the Rhesus D protein

Blood ◽  
2000 ◽  
Vol 96 (13) ◽  
pp. 4011-4019 ◽  
Author(s):  
Lisa-Marie Stott ◽  
Robert N. Barker ◽  
Stanislaw J. Urbaniak
Keyword(s):  
T Cell ◽  
Mononuclear Cells ◽  
Complete Sequence ◽  
Negative Control ◽  
T Cell Response ◽  
T Cell Epitopes ◽  
Considerable Effort ◽  
Peripheral Blood Mononuclear ◽  
Histocompatibility Complex ◽  
Alloreactive T Cell

Although considerable effort has been devoted to characterizing alloantibodies specific for the Rhesus D (RhD) blood group antigen, virtually nothing is known about the helper response that drives their production. Therefore, the aim of this study was to map alloreactive T-cell epitopes on the RhD protein. Peripheral blood mononuclear cells (PBMCs) were obtained from 22 RhD-negative volunteers in whom anti-D alloantibodies had developed after deliberate immunization or RhD-incompatible pregnancy. The PBMCs were stimulated with a panel of up to 68 overlapping synthetic 15-mer peptides spanning the complete sequence of the RhD protein. One or more peptides elicited proliferative responses by PBMCs from all 22 of the alloimmune volunteers but from only 2 of 8 alloantibody-negative control donors. Proliferation of PBMCs from the alloimmune donors was mediated by major histocompatibility complex class II–restricted T cells expressing the CD45RO marker of previous activation or memory. The number of peptides that induced proliferative responses was unrelated to either the frequency of, or time since, exposure to RhD-positive red blood cells, but it correlated strongly (Rs = 0.75;P < .003) with the level of anti-D antibodies in deliberately immunized donors. The patterns of stimulatory peptides varied among alloimmune volunteers, but particular sequences were commonly recognized, with 4 peptides each eliciting a response in more than 50% of these donors. Identification of such peptides containing dominant alloreactive helper epitopes is the first step in the development of improved or new approaches to preventing hemolytic disease of the newborn that are based on modulating the T-cell response to the RhD protein.

Thermosensitivity of Bos indicus cattle and their F1, crosses with three breeds of Bos taurus

1991 ◽  
Vol 52 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Khub Singh ◽  
N. K. Bhattacharyya
Keyword(s):  
Ambient Temperature ◽  
Rectal Temperature ◽  
Heat Production ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Metabolizable Energy ◽  
Ambient Temperatures ◽  
Brown Swiss ◽  
Genetic Groups ◽  
Holstein Friesian

ABSTRACTResting heat production (H), respiratory rate (RR) and rectal temperature (Tr) were measured at different controlled temperatures (Tt) in Hariana (Bos indicus) and its F, crosses with Jersey (JH), Brown Swiss (BH) and Holstein Friesian (FH) (Bos taurus) breeds and the values obtained were used to assess their relative thermosensitivity.The lowest Tt at which H significantly decreased from that at 17°c was 32°c for Hariana, JH and BH and 27°c for FH after exposure for 8 days. The corresponding values after exposure for 18 days were 37°c for Hariana and 32°c for all the three crossbred groups. Differences between the genetic groups were also significant. The lowest Tt at which metabolizable energy (ME) decreased significantly in comparison with those at 17CC was 32°c in all the genetic groups. The differences in ME intake between genetic groups were significant only at 32°c Tt. The lowest Tt at which RR significantly increased from those at 17°c were 32°c in Hariana, 27°c in JH, BH and FH for both 5 to 7 and 15 to 17 days of exposure. The corresponding Tt for increase in Tr was 37°c in Hariana, 32°c in JH and 27°c in BH and FH at both 5 to 7 and 15 to 17 days of exposure.The ambient temperature at which H would have significantly decreased and RR and Tr increased from the respective values at 17CC Tt were calculated curvilinearly for different genetic groups. There were differences in these values of calculated ambient temperatures between genetic groups and between exposure durations in respect of H, RR, and Tr, indicating differences in thermosensitivity.

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