Heat Shock Protein70 (HSP70) Gene Expression Pattern in Peripheral Blood Mononuclear Cells (PBMCs) during different Seasons in Sahiwal Cows (Bos Indicus)

2015 ◽  
Vol 5 (1) ◽  
pp. 109
Author(s):  
Mehtab S. Parmar ◽  
A.K. Madan ◽  
Ruokuobeinuo Huozha ◽  
S.K. Rastogi ◽  
Bhabesh Mili
Keyword(s):  
Gene Expression ◽  
Heat Shock ◽  
Mononuclear Cells ◽  
Bos Indicus ◽  
Gene Expression Pattern ◽  
Hsp70 Gene ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Sahiwal Cows ◽  
Different Seasons

Selected Contribution: Effect of acute heat shock on gene expression by human peripheral blood mononuclear cells

2002 ◽  
Vol 92 (5) ◽  
pp. 2208-2220 ◽  
Author(s):  
Larry A. Sonna ◽  
Stephen L. Gaffin ◽  
Richard E. Pratt ◽  
Michael L. Cullivan ◽  
Karen C. Angel ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heat Shock ◽  
Peripheral Blood Mononuclear Cells ◽  
Heat Shock Response ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Shock Response ◽  
Blood Mononuclear Cells

We studied the effect of heat shock on gene expression by normal human cells. Peripheral blood mononuclear cells (PBMCs) were obtained from healthy adults. Paired samples from each subject were subjected to either 20 min of heat shock (43°C) or control (37°C) conditions and then returned to 37°C. RNA was isolated 160 min later, and five representative samples were analyzed on Affymetrix gene chip arrays containing ∼12,600 probes. A biologically meaningful effect was defined as a statistically significant, twofold or greater difference in expression of sequences that were detected in all five experiments under control (downregulated sequences) or heat shock (upregulated sequences) conditions. Changes occurred in 395 sequences (227 increased by heat shock, 168 decreased), representing 353 Unigene numbers, in every functional category previously implicated in the heat shock response. By RT-PCR, we confirmed the findings for one upregulated sequence (Rad, a G protein) and one downregulated sequence (osteopontin, a cytokine). We conclude that heat shock causes extensive gene expression changes in PBMCs, affecting all functional categories of the heat shock response.

Sign in / Sign up

Export Citation Format

Share Document