PCR-RFLP Polymorphism of Growth Hormone Gene and Its Association with Growth and Morphometric Traits in Ganjam and Baigani Goats of Odisha

2020 ◽  
Author(s):  
D. K. Karna ◽  
A. Aradhana ◽  
G. D. Nayak ◽  
N. Sahoo ◽  
L. K. Sahoo ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Genetic Variation ◽  
Restriction Enzyme ◽  
Gene Segment ◽  
Growth Hormone Gene ◽  
Morphometric Traits ◽  
Exon 2 ◽  
Genotype Frequencies ◽  
Significant Difference ◽  
Pcr Rflp

Background: Growth Hormone (GH) gene codes for the growth hormone, an anabolic hormone synthesized and secreted by the somatotroph cells of pituitary. Growth hormone influences many processes such as growth, lactation, reproduction and metabolism. Genetic variation in the gene are utilized as markers for selecting animals that are superior in terms growth, production and reproduction traits. Information available on the polymorphism of growth hormone gene of Ganjam and Baigani goats of Odisha is scanty. The current investigation was done to explore the genetic variation of this gene and its association with morphometric traits. Methods: Genetic polymorphism in exon 2 and 3 of Growth hormone gene in Ganjam goat and Baigani goat was explored with sample size of 100 goats for each. The goats belonged to three locations: Khallikote, Rambha, Chattrapur of Ganjam district. The goats were recorded for their body weights, morphometric traits and morphological traits. Genomic DNA was isolated, the target segment comprising exons 2 and 3 was amplified and PCR-RFLP was carried out using Hae III restriction enzyme. Genotypes were scored. Result: PCR of the locus resulted in 422 bp PCR product. PCR-RFLP using Hae III restriction enzyme yielded only two variants in both the populations. The variant A had only one restriction recognition site on the target gene segment yielding two bands with size of 366bp and 56bp whereas the variant B did not have any restriction site with single band of 422bp. Three genotypes AA, AB and BB were found in both the population. In both the population, the gene and genotype frequency were significantly deviated from the Hardy Weinberg Equilibrium frequency. There was a significant difference in the genotype frequencies of growth hormone gene between Ganjam and Baigani goats. The AB genotype had higher mean value for all morphometric traits than AA and BB genotype though the differences were not found to be significant.

Polymorphism Of Exon 2-3 Of Growth Hormone Gene In Surti and Mehsani Goats by PCR-RFLP

2018 ◽  
Vol 8 (5) ◽  
pp. 1
Author(s):  
Jyotishree Bayan ◽  
Vishnu Kharadi ◽  
Umed Ramani ◽  
Mamta Janmeda ◽  
Pawar Vallabh ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Hormone Gene ◽  
Exon 2 ◽  
Pcr Rflp

scholarly journals IDENTIFIKASI POLIMORFISME GEN GH (GROWTH HORMONE) SAPI BALI DENGAN METODE PCR-RFLP

2006 ◽  
Vol 12 (1) ◽  
pp. 7-11 ◽  
Author(s):  
Sri Rahayu ◽  
Sutiman Bambang Sumitro ◽  
T Susilawati ◽  
Soemarno Soemarno
Keyword(s):  
Growth Hormone ◽  
Restriction Enzyme ◽  
Growth Hormone Gene ◽  
Salting Out ◽  
Pcr Product ◽  
Pcr Rflp ◽  
Reverse Primer ◽  
Total Dna ◽  
Polymerase Chain ◽  
Forward Primer

This study was conducted to identify polymorphism of growth hormone gene of Bali cattle. A PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) procedure was developed for determining polymorphism of growth hormone gene. The DNA was isolated from blood samples by salting out method. Total DNA were amplified with forward primer, 5’-TAGGGGAGGGTGGAAAATGGA-3’ and reverse primer, 5’-GACACCTACTCAGACAATGCG-3’. The PCR product was digested by HaeIII restriction enzyme. Result of the amplification was a specific single band with fragment 450 bp. Restriction with HaeIII restriction enzyme resulted four kinds of haplotype. Haplotype I was not cut by HaeIII restriction enzyme. Haplotype II were cut into two, 225 bp and 150 bp,. Haplotype III were cut into three size, 400 bp, 225 bp and 150 bp. Haplotype IV were cut into five fragments 450 bp, 400 bp, 275 bp, 225 bp and 150 bp.

scholarly journals Büyüme Hormonu Geni Polimorfizminin Buzağı Doğum Ağırlığı Üzerine Etkisi

2018 ◽  
Vol 5 (13) ◽  
pp. 1781
Author(s):  
Zeynep Sönmez ◽  
Memiş Özdemir ◽  
Vecihi Aksakal
Keyword(s):  
Growth Hormone ◽  
Genetic Variation ◽  
Birth Weight ◽  
Frequency Distribution ◽  
Significant Relationship ◽  
Growth Hormone Gene ◽  
Balance Test ◽  
Pcr Rflp ◽  
Holstein Calves

The aim of study were to present relationships between birth weight of 94 Holstein calves with genotype structures of each sample’s Growth Hormone (GH) determined through PCR-RFLP method and to detect the population’s genetic variation by determining the genotype and allel frequency distribution. In the study, according to the analysis result, it was determined that Growth Hormone gene LL genotype was 41.9 kg, LL was 40.8 kg, and VV was 42.2 kg for the birth weight. A significant relationship between birth weight with GH genotypes could not be found. It was seen that the calf population is not stable according to Hardy-Weinberg genetic balance test.

scholarly journals Growth Hormone Gene Polymorphism and its Relation to Quail Growth and Carcass

2021 ◽  
Vol 9 (8) ◽  
pp. 1395-1397
Author(s):  
Fatma İlhan
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Gene Polymorphism ◽  
Restriction Enzyme ◽  
Carcass Traits ◽  
Growth Hormone Gene ◽  
Japanese Quails ◽  
Intron 1 ◽  
Pcr Rflp ◽  
Gh Gene

In this study, it was aimed to determine the polymorphism of GH (growth hormone) gene in Japanese quails and the relationships between these genes and body weight and carcass traits. 3 genotypes (AA, AB and BB), 2 (A and B) alleles were detected by cutting the GH intron 1 region with restriction enzyme MspI. As a result of variance analysis, it was determined that the hatching weights of the animals with B allele and liver weights were higher. Thus, it is seen that GH gene and PCR-RFLP technique can be used in breeding studies.

scholarly journals Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia

2018 ◽  
Vol 61 (4) ◽  
pp. 481-489 ◽  
Author(s):  
Sihem Amiri ◽  
Bayrem Jemmali ◽  
Mohamed Amine Ferchichi ◽  
Hajer Jeljeli ◽  
Rekik Boulbaba ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Dairy Cattle ◽  
Reproductive Traits ◽  
Mixed Linear Model ◽  
Favorable Effect ◽  
Growth Hormone Gene ◽  
Nucleotide Polymorphisms ◽  
Pcr Rflp ◽  
The Difference ◽  
Bovine Species

Abstract. Research to assess the effect of single genes on reproductive traits in bovine species is imperative to elucidate genes' functions and acquire a better perspective of quantitative traits. The present study was undertaken to characterize genetic diversity in the bovine growth hormone (GH) gene in a population of 410 Holstein dairy cows in Tunisia. The analyses were based on single nucleotide polymorphisms, and GH-AluI and GH-MspI detections and genotyping were carried out using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Data were analyzed using a mixed linear model with the MIXED procedure to reveal the possible effect of GH genotypes on reproductive traits. The frequency data of AluI(L//V) and MspI(+//−) alleles were 87.04//12.96 and 70.06//29.94, respectively. The distribution of the frequency of GH genotypes for LL/LV/VV and (−/−)//(+/−)//(+/+) were 77.75//18.59//3.66 and 15.37//29.13//55.50, respectively. The results of the statistical analyses proved that GH-AluI showed a substantial favorable effect on exanimate traits except for the age at first calving; however, only a suggestive effect of GH-MspI on the calving interval (CI) and the days open (DI) was found. The homozygous LL genotype seemed to be advantageous with respect to the CI and the DI compared with LV and VV genotypes. Heterozygous MspI(+/−) cows tended to have a longer CI and DI than MspI(+/+) and MspI(−/−) cows, but the difference was not statistically significant. A significant effect of different GH-AluI–MspI combined genotypes was found on the number of inseminations per conception, the CI and the DI, and the LL/– combined genotype seemed to be associated with better reproductive performance. Based on these results, the LL genotype of the GH locus can be considered to be a favorable genotype for reproductive traits in Holstein dairy cattle, although these findings need to be confirmed by further research before polymorphisms can be used in a marker-assisted selection program.

scholarly journals Associations between the AluI polymorphism of growth hormone gene and production and reproduction traits in a Hungarian Holstein-Friesian bull dam population

2006 ◽  
Vol 49 (3) ◽  
pp. 236-249 ◽  
Author(s):  
K. Kovács ◽  
J. Völgyi-Csík ◽  
A. Zsolnai ◽  
I. Györkös ◽  
L. Fésüs
Keyword(s):  
Growth Hormone ◽  
Milk Fat ◽  
Milk Composition ◽  
Statistical Analyses ◽  
Bovine Growth Hormone ◽  
Growth Hormone Gene ◽  
Genotype Distribution ◽  
Holstein Friesian ◽  
Pcr Rflp ◽  
Reproduction Traits

Abstract. The aim of this paper was to study the polymorphisms of bovine growth hormone gene. The authors genotyped 363 Hungarian Holstein-Friesian bull dams from 6 farms all over the country. Two variants (L and V) of the bovine growth hormone gene digested with AluI enzyme were identified in the experiment. Genotyping was carried out by PCR-RFLP method. The frequency data of L and V allele was 0.93 and 0.07 respectively. Distribution of the three genotypes were 87.05% (LL), 12.40% (LV) and 0.55% (VV). The studied population was in H-W equilibrium considering the genotype distribution. SPSS 11.0 for Windows was used to reveal the possible correlations between GH genotypes and production and reproduction traits and further statistical analyses. On the basis of statistical analyses it can be found that VV genotype cows had the longest milking period and LL had the shortest dry period. Both differences were significant. Cows with LV genotype had significantly higher test milking data than LL cows. Furthermore, LV genotype seemed to be advantageous for 305 days lactation milk yield. While milk composition traits, as 305 days milk fat and protein percent showed the opposite tendency, since LL genotyped dams produced significantly higher values in these traits.

scholarly journals Mir-125a rs12976445 is Associated with Susceptibility to Thyroid Cancer: A Case-control Study

2020 ◽  
Author(s):  
Nima Nima Montazeri-Najafabady ◽  
Mohammad Hossein Dabbaghmanesh ◽  
Abbas Ghaderi ◽  
Nazanin Chatrabnous ◽  
Mohammad Reza Arabnezhad ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Case Control ◽  
Occurrence Rate ◽  
Cancer Type ◽  
Dominant Model ◽  
Allele Distribution ◽  
Genotype Frequencies ◽  
Significant Difference ◽  
Pcr Rflp ◽  
Cancer Types

Abstract Background: Thyroid cancer is the fifth communal cancer type in females and its occurrence rate continues to rise rapidly worldwide. Latest data demonstrated mir-125 is down-regulated in various cancer types. Methods: a case-control (179 cases, 165 controls) study in order to explore the association of mir-125 rs12976445 with the risk of thyroid cancer in the Iranian population was performed. In order to investigate rs12976445 C/T polymorphisms, polymerase chain reaction restriction–fragment length polymorphism (PCR–RFLP) was done. Logistic regression analyses were done to find the association of mir-125 rs12976445 C/T polymorphisms with thyroid cancer and its stages. Results: The genotype frequencies for patients was [(CC: 81(45.2%), CT: 75(41.9%), TT: 23 (12.9%)], and for controls was [(CC: 100 (60.1%), CT: 53(32.2%), TT: 12 (6.7%)]. The T allele distribution was significantly altered between patients and controls (P=0.002) with the odds ratio of 1.68. In the co-dominant model CC genotype was set as reference and compared with CT, and TT genotypes. In the dominant model, there was a significant difference between CC vs CT genotypes (adjusted OR = 1.69, 95% CI= 1-2.8, P = 0.026), and slightly significant differences between CC vs TT genotypes (adjusted OR = 2.18, 95% CI= 1-4.7, P = 0.047). we compared CT/TT genotype to the reference genotype (CC) and found a highly significant difference (adjusted OR = 1.78, 95% CI= 1.15-2.74, P = 0. 0.009).Conclusion: as the first study, our findings suggest that miR-125a rs12976445 is a possible prognostic biomarker for thyroid cancer patients.

Sign in / Sign up

Export Citation Format

Share Document