Identification and sequence characterization of LOC427400: a novel gene highly expressed in the Magnum of White leghorn’s Oviduct

2018 ◽  
Author(s):  
Ying Zhang ◽  
Bolin Ning ◽  
Jingchun Li ◽  
Qianyu He ◽  
Chunbo Wei ◽  
...  
Keyword(s):  
Chicken Genome ◽  
Carbohydrate Binding ◽  
Biological Functions ◽  
White Leghorn ◽  
Quantitative Real Time Pcr ◽  
Sequence Characterization ◽  
Chicken Oviduct ◽  
Egg Albumen ◽  
Different Parts

LOC427400 is a novel bird gene located on chromosome Z of the chicken genome. It was deciphered in our previous study by RNA sequencing of different parts of the chicken oviduct. Here we confirmed that LOC427400 expressed significantly high in the magnum comparing with the ovary, isthmus, uterus, liver, jejunum and breast muscle (p Lass Than 0.05) by quantitative real-time PCR in 5 White Leghorn layers at the age of 34 wk. The full length of transcript sequence of LOC427400 was also obtained by 3’ and 5’ RACE. The new transcript was 2831 bp and the corresponding translated protein was 343 aa. The predicted protein show 2 conserved domains, RICIN and OST-beta, which indicated that LOC427400 protein might have functions like carbohydrate-binding and signal transduction. This study illustrated that LOC47400 should be an important candidate gene related with the process of egg albumen formation and albumen biological functions.

scholarly journals Characterization of a Red Jungle Fowl by White Leghorn Backcross Reference Population for Molecular Mapping of the Chicken Genome

1993 ◽  
Vol 72 (2) ◽  
pp. 334-348 ◽  
Author(s):  
LYMAN B. CRITTENDEN ◽  
LEONARD PROVENCHER ◽  
LISA SANTANGELO ◽  
ILAN LEVIN ◽  
HANS ABPLANALP ◽  
...  
Keyword(s):  
Chicken Genome ◽  
Molecular Mapping ◽  
Reference Population ◽  
White Leghorn ◽  
Red Jungle Fowl

Whole-Genome Sequence Characterization of Primary Auxin-Responsive Aux/IAA Gene Family in Sorghum (Sorghum bicolor L.)

2010 ◽  
Vol 36 (4) ◽  
pp. 688-694
Author(s):  
Yi-Jun WANG ◽  
Yan-Ping LÜ ◽  
Qin XIE ◽  
De-Xiang DENG ◽  
Yun-Long BIAN
Keyword(s):  
Sorghum Bicolor ◽  
Gene Family ◽  
Genome Sequence ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Sequence Characterization ◽  
I Gene

Molecular Characterization of Rotavirus Porcine OSU Carbohydrate- Binding Domain VP8*

2015 ◽  
Vol 12 (1) ◽  
pp. 69-72
Author(s):  
Hao Li ◽  
Wei Zhang ◽  
Hong-hao Zhou ◽  
Xiao-li Li
Keyword(s):  
Molecular Characterization ◽  
Binding Domain ◽  
Carbohydrate Binding

Highly precise characterization of the hydration state upon thermal denaturation of human serum albumin using a 65 GHz dielectric sensor

2020 ◽  
Vol 22 (35) ◽  
pp. 19468-19479 ◽  
Author(s):  
Keiichiro Shiraga ◽  
Mako Urabe ◽  
Takeshi Matsui ◽  
Shojiro Kikuchi ◽  
Yuichi Ogawa
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Thermal Denaturation ◽  
Hydration Water ◽  
Biological Functions ◽  
Dielectric Sensor ◽  
Hydration State ◽  
Precise Characterization

The biological functions of proteins depend on harmonization with hydration water surrounding them.

scholarly journals LncRNA NEAT1 Enhances Glioma Progression via Regulating the miR-128-3p/ITGA5 Axis

2021 ◽  
Author(s):  
Jiakai Chen ◽  
Handong Wang ◽  
Junjun Wang ◽  
Wenhao Niu ◽  
Chulei Deng ◽  
...  
Keyword(s):  
Flow Cytometric Analysis ◽  
Biological Functions ◽  
Quantitative Real Time Pcr ◽  
Flow Cytometric ◽  
Non Coding Rna ◽  
Rna Immunoprecipitation ◽  
Human Gbm ◽  
Novel Strategy ◽  
Lncrna Neat1 ◽  
Long Non Coding Rna

AbstractAccumulating evidences indicate that long non-coding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) promotes the progression of glioma. In this study, we postulated that NEAT1 may act as a miR-128-3p sponge. Relative levels of NEAT1 and miR-128-3p expression in human glioma samples and GBM cells were detected using quantitative real-time PCR. By means of CCK-8 assays, transwell assays, and flow cytometric analysis, the biological functions of miR-128-3p and NEAT1 were investigated in U87MG and U251MG human GBM cell lines with stable miR-128-3p and NEAT1 knockdown or overexpression. The luciferase reports, RNA pull-down assay, and RNA immunoprecipitation assay were conducted to determine the relevance of NEAT1 and miR-128-3p in glioma. As a result, high expression of NEAT1 and lack of miR-128-3p were observed in glioma specimens and cells. By binding to anti-oncogene miR-128-3p in the nucleus, NEAT1 enhanced tumorigenesis and glioma development. Further experiments suggested that ITGA5 expression was increased in glioma tissues and was found to be connected with miR-128-3p. Additionally, NEAT1 facilitated ITGA5 expression via competitively binding to miR-128-3p. For this reason, ITGA5 would not be decomposed by miR-128-3p and could activate FAK signaling pathway, thereby promoting cell growth. Collectively, these results indicated that the NEAT1/miR-128-3p/ITGA5 axis was involved in glioma initiation and progression, and might offer a potential novel strategy for treatment of glioma.

scholarly journals Transcriptome-based identification and characterization of genes responding to imidacloprid in Myzus persicae

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jianyu Meng ◽  
Xingjiang Chen ◽  
Changyu Zhang
Keyword(s):  
Genetic Basis ◽  
Expression Profiles ◽  
Control Measure ◽  
Effective Control ◽  
Agricultural Pest ◽  
Functional Classification ◽  
Quantitative Real Time Pcr ◽  
Underlying Mechanisms ◽  
Identification And Characterization

Abstract Myzus persicae is a serious and widespread agricultural pest, against which, imidacloprid remains an effective control measure. However, recent reports indicate that this aphid has evolved and developed resistance to imidacloprid. This study aimed to elucidate the underlying mechanisms and genetic basis of this resistance by conducting comparative transcriptomics studies on both imidacloprid-resistant (IR) and imidacloprid-susceptible (IS) M. persicae. The comparative analysis identified 252 differentially expressed genes (DEGs) among the IR and IS M. persicae transcriptomes. These candidate genes included 160 and 92 genes that were down- and up-regulated, respectively, in the imidacloprid-resistant strain. Using functional classification in the GO and KEGG databases, 187 DEGs were assigned to 303 functional subcategories and 100 DEGs were classified into 45 pathway groups. Moreover, several genes were associated with known insecticide targets, cuticle, metabolic processes, and oxidative phosphorylation. Quantitative real-time PCR of 10 DEGs confirmed the trends observed in the RNA sequencing expression profiles. These findings provide a valuable basis for further investigation into the complicated mechanisms of imidacloprid resistance in M. persicae.

Sequence Characterization of Heat Shock Protein Gene ofCyclospora cayetanensisIsolates from Nepal, Mexico, and Peru

2013 ◽  
Vol 99 (2) ◽  
pp. 379-382 ◽  
Author(s):  
Irshad M. Sulaiman ◽  
Patricia Torres ◽  
Steven Simpson ◽  
Khalil Kerdahi ◽  
Ynes Ortega
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Gene ◽  
Heat Shock Protein Gene ◽  
Sequence Characterization

scholarly journals Carbohydrate binding activities of Bradyrhizobium japonicum. II. Isolation and characterization of a galactose-specific lectin.

1990 ◽  
Vol 111 (4) ◽  
pp. 1639-1643 ◽  
Author(s):  
S C Ho ◽  
M Schindler ◽  
J L Wang
Keyword(s):  
Isoelectric Focusing ◽  
Bradyrhizobium Japonicum ◽  
Affinity Column ◽  
Carbohydrate Binding ◽  
Binding Affinities ◽  
Isolation And Characterization ◽  
Relative Binding ◽  
Mannose Derivatives ◽  
Derivatives Of

Extracts of Bradyrhizobium japonicum were fractionated on Sepharose columns covalently derivatized with lactose. Elution of the material that was specifically bound to the affinity column with lactose yielded a protein of Mr approximately 38,000. Isoelectric focusing of this sample yielded two spots with pI values of 6.4 and 6.8. This protein specifically bound to galactose-containing glycoconjugates, but did not bind either to glucose or mannose. Derivatives of galactose at the C-2 position showed much weaker binding; there was an 18-fold difference in the relative binding affinities of galactose versus N-acetyl-D-galactosamine. These results indicate that we have purified a newly identified carbohydrate-binding protein from Bradyrhizobium japonicum, that can exquisitely distinguish galactose from its derivatives at the C-2 position.

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