ORAL CAVITY MICROBIOCENOSIS ASSESSMENT ON THE BASIS OF BACTERIAL ENDOTOXIN AND PLASMALOGENS IN A SALIVA BY METHOD GAS-LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY

2019 ◽  
Vol 64 (3) ◽  
pp. 186-192
Author(s):  
M. V. Samoylova ◽  
T. F. Kosyreva ◽  
A. E. Anurova ◽  
R. A. Abramovich ◽  
A. Yu. Mironov ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Pathological Process ◽  
Bacterial Endotoxin ◽  
Gas Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Intermediate Type ◽  
Microbial Composition ◽  
Periodontal Tissues ◽  
Inflammatory Processes

The concentration of plasmalogen bacterial and endotoxin levels in the saliva of patients with different severity of periodontal disease, injury prosthetic bed and with various degrees of the oral cavity microbiocenosis violations was studied. Determination of the presence of the pathological process was carried out clinically, according to the condition of periodontal tissues. The degree of microbiological disorders was assessed by the quantitative ratio of the types of microorganisms isolated from the smear taken from the gingival groove. It was found that the concentration of plasmalogen for normal microbiocenosis is not less than 0.7 µg/g. For the intermediate type of microbiocenosis, the concentration of 1.82 µg/g was determined; for dysbiosis - 5.64 µg/g, and for the expressed violation of the microbial composition accompanied by inflammatory processes - 6.54 µg/g. An increase in the concentration of bacterial endotoxin (be) more than 6.25 nanomole/g indicates the pronounced inflammatory process, regardless of the determined intensity of contamination of opportunistic gram-negative microflora.

Determination of the prevalence of helicobacter pylori oral infection in smoking patients with chronic generalized periodontitis on the background of chronic hyperacidal gastritis during treatment

2020 ◽  
Vol 40 (4) ◽  
pp. 50-54
Author(s):  
O. L. Zolotukhina ◽  
◽  
Ju. G. Romanova ◽  
O. V. Maslov ◽  
◽  
...  
Keyword(s):  
Risk Factors ◽  
Helicobacter Pylori ◽  
Oral Cavity ◽  
Urease Activity ◽  
Rapid Test ◽  
Oral Infection ◽  
Helicobacter Pylori Infection ◽  
Natural Origin ◽  
Periodontal Tissues

Diseases of periodontal tissues occupy one of the leading positions among modern dental problems, namely the multifactorial nature of these diseases. In modern dental science, the issue of the development of periodontal pathology against the background of somatic pathology and risk factors remains relevant. Pathology of periodontal tissues in 68–90 % of cases is accompanied by chronic diseases of the gastrointestinal tract. Today, there is no doubt that Helicobacter pylori infection can be present in the biotopes of the oral cavity and can affect the course of periodontal pathology. As you know, smoking is one of the important risk factors for the development of inflammatory-dystrophic diseases of periodontal tissues, which can aggravate the course of the latter. The purpose of the work is to determine the prevalence of oral Helicobacter pylori infection in tobacco-dependent patients with chronic generalized periodontitis on the background of chronic hyperacid gastritis during treatment. Patients who received the proposed therapeutic and prophylactic complex (ultraphonophoresis procedures with the created gel «Apisan», and probiotic drug BioGaia ProDentis and angioprotective drug of natural origin — Detralex) showed a gradual decrease in the level of total urease activity and, as a consequence, a decrease the prevalence of Helicobacter pylori infection in the oral cavity according to the results of a urease rapid test with material from the oral cavity, both in the presence of a risk factor — smoking, and in its absence. The use of the proposed therapeutic and prophylactic complex proved to be effective in reducing the prevalence of oral Helicobacter pylori infection in smoking patients and patients who do not smoke, with chronic generalized periodontitis against the background of chronic hyperacidal gastritis associated with Helicobacter pylori.

scholarly journals Microbiological aspects in conservative treatment of generalized periodontitis using autotrombocytic mass

2020 ◽  
Vol 3 (11) ◽  
pp. 206-209
Author(s):  
Turaeva F.A.
Keyword(s):  
Conservative Treatment ◽  
Oral Cavity ◽  
Immune Status ◽  
Pathological Process ◽  
The Body ◽  
Primary Factor ◽  
Normal Microflora ◽  
Inflammatory Processes ◽  
Microbiological Aspects

The primary factor causing periodontal damage is plaque bacteria. The etiological structure of infectious processes in the last decade has changed significantly, due to the constant evolution of microbes and the involvement of opportunistic microbes in the pathological process, which can act as commensals in the normal microflora and manifest their pathogenicity with a decrease in the body's immune status. Inflammatory processes in the oral cavity are sometimes an endogenous infection caused by the resident flora of not only the oral cavity, but also other ecosystems of the body.

scholarly journals Herbicides from the group of nitrodiphenyl ethers: applications in Ukraine, control methods, methods for determination of oxifluorphene residues in soybean grain and oil

2021 ◽  
Vol 20 (66) ◽  
pp. 15-23
Author(s):  
Yu Baranov ◽  
◽  
V Demchenko ◽  
Ie Zajets ◽  
S Olszewski ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Selective Extraction ◽  
Agricultural Products ◽  
Gas Liquid Chromatography ◽  
Control Methods ◽  
Liquid Chromatography Mass Spectrometry ◽  
Analytical Validation ◽  
Regulatory Requirements ◽  
Food And Feed

A review of analytical methods for the determination of herbicide residues from the group of nitrodiphenyl ethers in the environment and agricultural products, developed and validated a method for oxyfluorfen determination in soybean grain and oil method GC/ECD, which meets regulatory requirements and solves the problem of toxicant control in the above matrices. The developed method uses modern methods of sample preparation, identification and quantification of oxyfluorfen (selective extraction, capillary gas-liquid chromatography (GC/ECD GC/MS). The method is validated according to EU requirements) (SANTE/12682/2019 quality control and analytical validation procedures for pesticide residues analysis in food and feed). Key words: gas-liquid chromatography, mass spectrometry, herbicides, nitrodiphenyl ethers, control, residual amounts.

scholarly journals CONDITIONS OF THE ORAL CAVITY STATUS IN YOUTH WITH ALIMENTARY-CONSTITUTIONAL FORM OF OBESITY

2019 ◽  
Vol 23 (1-2) ◽  
pp. 17-21
Author(s):  
M. Skrypnyk ◽  
T. Petrushanko ◽  
T. Kryvoruchko ◽  
K. Neporada
Keyword(s):  
Oral Cavity ◽  
Periodontal Disease ◽  
Periodontal Diseases ◽  
Young Patients ◽  
Overweight And Obesity ◽  
Obesity Prevalence ◽  
Periodontal Tissues ◽  
Mild Degree ◽  
Inflammatory Processes ◽  
Second Degree

Obesity prevalence has significantly increased especially in young adults, which is caused by a particular lifestyle, food quality and dietary behavior. Obesity leads to development of huge array of comorbid conditions such as arterial hypertonia, heart stroke, arthritis and other diseases. We conducted standard clinical examination of oral cavity of 154 young patients (18-21 years old) – all of them were students of Ukrainian Medical Stomatological Academy. It has been determined that the majority of patients with BMI >30 kg/cm2 have aggravated family heredity, in 66,2% cases one parent of obese students had obesity and 32,43% patients have both parents with obesity that is significantly higher compared with persons with normal BMI and overweight. There was a high prevalence of periodontal diseases about 74% and carious lesions of teeth - 97.4% among young people despite the age and contingent of examined patients - medical students. In patients with BMI >30 kg/cm2 prevalence of generalized forms of gingivitis and periodontitis was by three times higher compared with individuals with normal BMI. The values of oral hygienic indexes were poor in all examined groups, but their values were slightly lower in patients with normal BMI than in those with overweight and obesity. The prevalence of inflammatory changes in gums was higher in persons with obesity: all of them had a mild degree of lesions in periodontal tissues. Inflammatory processes in the gums were the most intense in patients with the second degree of obesity. According to the results of the study, the presence of the first and the second degree of obesity should be considered as a risk factor triggering periodontal tissues diseases. For persons with BMI >30 kg/cm2 with periodontal disease measures for the secondary prevention of inflammatory and inflammatory dystrophic periodontal diseases should be carried out and in persons without periodontal disease on the background of obesity measures primary prevention should be done.

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