THE EFFECT OF MICROWAVES OF A DECIMETER RANGE ON THE FUNCTIONAL ACTIVITY OF MITOCHONDRIA IN DESTRUCTIVE APPENDICITIS IN CHILDREN

Introduction. The article assessed the effectiveness of decimeter wave therapy (DWT) in the postoperative period after laparoscopic appendectomy for destructive appendicitis in children. Authors relate positive clinical effects with the activation of mitochondrial energy metabolism. Material and methods. The study included 132 patients (46 destructive appendicitis cases, 86 h appendicular peritonitis patients). Among them, there were 75 (56.8 percent) boys and 57 (43.2 percent) girls aged of from 3 to 17 years (mean age of 10.7 ± 3.07 years). Patients of the main group received DWT procedures with the use of the apparatus DMV-02 “Solnyshko” starting from the 1st day after the surgery. Patients from the reference group received no physiotherapy treatment. The activity of dehydrogenases of lymphocytes (succinic dehydrogenase (SDH) and NADH-dehydrogenase) in patients from the main group and the comparison group were determined by means of cytomorphological method with the use a hardware-software visualization complex “Videotest” and the “Morphology 5.2” (Russia), at the 1st, 3rd and 5th day of the postoperative period. Results. Under the influence of DWT in the postoperative period the frequency of intestinal insufficiency syndrome, systemic inflammatory response syndrome, infiltrative adhesions was established to decline. There was noted a gain in the activity of mitochondrial enzymes, reflecting the first and second stages of the respiratory chain (SDH and NADH-dehydrogenase). In children with destructive appendicitis against the background of DWT, the normalization of the activity of LDH in moderate activation of NADH-dehydrogenase, and in appendicular peritonitis cases - the significant elevation in LDH activity of lymphocytes and activation of NADH-dehydrogenase occurs. In patients from the main group, the absolute number of peripheral blood lymphocytes normalized Conclusion. Under the influence of DWT there was noted the activation of enzymes of mitochondria that provides a mild course of the postoperative period.

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Application of Nasomer in surgical treatment of patients with polyposis rhinosinusitis

OTORHINOLARYNGOLOGY 2-3(2) 2019 - OTORHINOLARYNGOLOGY ◽

10.37219/2528-8253-2018-2-39 ◽

2018 ◽

pp. 39-42

Author(s):

Vladimir Bereznyuk ◽

Alexander Chernokur ◽

Oleg Gospod

Keyword(s):

Nasal Cavity ◽

Postoperative Period ◽

Paranasal Sinuses ◽

Standard Treatment ◽

Surgical Intervention ◽

Sodium Hyaluronate ◽

Main Group ◽

Control Group ◽

Nasal Breathing ◽

Minimal Invasiveness

Relevance: Modern endonasal surgery allows to remove polyps from all affected paranasal sinuses, following the principles of minimal invasiveness. Minimal traumatic of surgical intervention gives the best results, accompanied by less progression of the disease. Minimal invasiveness of surgical intervention and its obligatory combination with postoperative medical treatment are common practice in many countries. One of the drugs that actively effect the restoration of the mucous membrane of the nasal cavity and paranasal sinuses in the early postoperative period is Nazomer, which includes sodium hyaluronate and dexpanthenol in saline solution. The purpose of the study is to investigate the effectiveness of the drug Nazomer in patients with polyposis rhinosinusitis after endoscopic polyposynosotomy. Results and discussion: The main group consisted of 30 patients with polyposis rhinosinusitis, who were prescribed Nasomer in addition to standard treatment in the postoperative period. The control group included 30 patients who underwent standard treatment in the postoperative period. As criteria for clinical efficacy, data from endoscopic examination of the nasal cavity and indicators of anterior rhinomatometry, measured by the «Optimus» device, were selected. In the main group, the index of nasal breathing according to rhinomatometry was better than the results of patients in the control group on the 3rd and 5th day of the postoperative period by 26% and 24%, respectively. Conclusion: The use of the drug Nazomer in the postoperative period in patients with polyposis rhinosinusitis contributes to more active restoration of respiratory function of the nasal cavity compared with the control group, according to rhinomatometry, up to 26%. Based on the results obtained, the drug Nazomer is an effective anti-inflammatory and regenerative agent in the postoperative period in patients with polyposis rhinosinusitis.

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Cytoprotective Effects of N-Acetylcysteine on Streptozotocin- Induced Oxidative Stress and Apoptosis in RIN-5F Pancreatic β-Cells

Cellular Physiology and Biochemistry ◽

10.1159/000495200 ◽

2018 ◽

Vol 51 (1) ◽

pp. 201-216 ◽

Cited By ~ 8

Author(s):

Arwa M.T. Al-Nahdi ◽

Annie John ◽

Haider Raza

Keyword(s):

Oxidative Stress ◽

Insulin Secretion ◽

Molecular Mechanisms ◽

Protective Action ◽

Mitochondrial Enzymes ◽

Partial Recovery ◽

Β Cells ◽

Pancreatic Β Cells ◽

Mitochondrial Energy Metabolism ◽

Mitochondrial Functions

Background/Aims: Numerous studies have reported overproduction of reactive oxygen species (ROS) and alterations in mitochondrial energy metabolism in the development of diabetes and its complications. The potential protective effects of N-acetylcysteine (NAC) in diabetes have been reported in many therapeutic studies. NAC has been shown to reduce oxidative stress and enhance redox potential in tissues protecting them against oxidative stress associated complications in diabetes. In the current study, we aimed to investigate the molecular mechanisms of the protective action of NAC on STZ-induced toxicity in insulin secreting Rin-5F pancreatic β-cells. Methods: Rin-5F cells were grown to 80% confluence and then treated with 10mM STZ for 24h in the presence or absence of 10mM NAC. After sub-cellular fractionation, oxidative stress, GSH-dependent metabolism and mitochondrial respiratory functions were studied using spectrophotometric, flow cytometric and Western blotting techniques. Results: Our results showed that STZ-induced oxidative stress and apoptosis caused inhibition in insulin secretion while NAC treatment restored the redox homeostasis, enhanced insulin secretion in control cells and prevented apoptosis in STZ-treated cells. Moreover, NAC attenuated the inhibition of mitochondrial functions induced by STZ through partial recovery of the mitochondrial enzymes and restoration of membrane potential. STZ-induced DNA damage and expression of apoptotic proteins were significantly inhibited in NAC-treated cells. Conclusion: Our results suggest that the cytoprotective action of NAC is mediated via suppression of oxidative stress and apoptosis and restoration of GSH homeostasis and mitochondrial bioenergetics. This study may, thus, help in better understanding the cellular defense mechanisms of pancreatic β-cells against STZ-induced cytotoxicity.

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MODERN METHODS OF PREVENTION AND TREATMENT OF COMPLICATIONS OF CERVICAL ESOPHAGO-ORGAN ANASTOMOSIS AT ESOPHAGOPLASTY

Wiadomości Lekarskie ◽

10.36740/wlek202008120 ◽

2020 ◽

Vol 73 (8) ◽

pp. 1696-1699

Author(s):

Volodymyr O. Shaprynskyi ◽

Yevhen V. Shaprynskyi ◽

Mustafa Bassam Hussein ◽

Oleg O. Vorovskyi ◽

Yaroslav V. Karyi ◽

...

Keyword(s):

Operative Treatment ◽

Postoperative Period ◽

Comparison Group ◽

Postoperative Mortality ◽

Diagnostic Algorithm ◽

Main Group ◽

Instrumental Method ◽

Complication Risk ◽

Esophageal Strictures ◽

Bed Days

The aim: To improve the results of operative treatment of esophageal strictures by decreasing the rate of failure and stricture of cervical esophago-organ anastomoses. Materials and methods: There were 45 patients with post-burn corrosive gullet strictures, 17 patients with postoperative corrosive strictures, 10 patients with peptic strictures secondary to reflux-esophagitis, 42 patients with esophageal cancer strictures. The patients were divided into two groups: the comparison group – 55 persons and the main group – 59 persons. Patients of comparison group underwent surgical treatment of esophageal strictures according to classic protocols and standards. In the main group of patients we applied proposed diagnostic algorithm with prediction of complication risk and the designed method of esophago-organ anastomosis formation. Results: The results of operative treatment in patients with esophageal strictures showed the development of early postoperative complications in 59 individuals (51.75 %). In the postoperative period six patients died: four – in the comparison group and two – in the main group. Failure of cervical esophago-organ anastomosis and esophageal strictures occurred in 7 patients (11.86 %) of main group and 20 patients (36.36 %) of the comparison group (p<0.05). Conclusions: Application of method predicting the risk of complications of cervical anastomosis, treatment program and instrumental method of formation anastomosis resulted in reduced incidence of failure and strictures of esophago-organ anastomosis from 36.36 % to 11.86 % (p<0.05); decreased time of hospitalization - from 28.2 ± 1.1 to 21.5 ± 0.5 bed-days (p<0.001), postoperative period - from 20.5 ± 1.1 to 16.1 ± 0.7 bed-days (p<0.01); decreased postoperative mortality - from 7.27 % to 3.39 %.

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Impact of the Pre-operative Chemoradiation Therapy on Complications in Patients with Locally Advanced Esophageal Cancer

Malignant tumours ◽

10.18027/2224-5057-2018-8-4-30-36 ◽

2019 ◽

Vol 8 (4) ◽

pp. 30-36

Author(s):

M. S. Shogenov ◽

M. M. Davydov ◽

A. K. Allakhverdiyev ◽

A. A. Filatov ◽

A. K. Chekini ◽

...

Keyword(s):

Mortality Rate ◽

Postoperative Period ◽

Locally Advanced ◽

Postoperative Management ◽

Early Postoperative Period ◽

Main Group ◽

Chemoradiation Therapy ◽

Thoracic Esophagus ◽

Complex Treatment ◽

Locally Advanced Tumors

The aim of this study was to evaluate the effect of the chemoradiation therapy on the incidence of postoperative complications and mortality rate in patients with locally advanced tumors of the thoracic esophagus.The study included men and women over 18 with histologically verified squamous cell carcinoma of the thoracic esophagus with TNM stage cT3-4aN0-3M0. Patients were divided into two groups: patients in a main group (n=26) received a complex treatment, patients in a second group (n=30) undergoing only surgical treatment.The first stage of treatment was performed for all 26 patients and included 2 courses of preoperative chemotherapy, followed by a chemoradiation therapy with a 44–46 Gy with weekly injections of chemotherapy. Surgical procedure (R0) was performed for 24 patients (92.3 %) in the main group and for 26 (86.6 %) in a second group.Therapeutic complications were developed in 17 (65 %) patients in a first group, while in a second group this rate was in 18 (60 %) patients from 30. The incidence of surgical complications was slightly higher in a group of patients received a complex treatment –7 (26.92 %) patients. In the surgical group such complications was noted in 6 (20 %) patients. The only death in an early postoperative period was in 1 (3.84 %) patient from the main group. The cause was sepsis and the development of multiple organic failure. There were no lethal outcomes in a surgical group.Estimating the incidence of complications, it should be noted that the use of chemoradiation therapy in the neoadjuvant regimen does not significantly affect the course of the postoperative period. The mortality rate was slightly higher in in the group of patients received complex treatment. At the same time, improvement of the ostoperative period and a reduction of mortality rate in group ofpatients undergoing complex treatment is possible due to optimization of preoperative preparation, postoperative management and competent selection of patients.

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Recombinant alpha 2-interferon in the treatment of B chronic lymphocytic leukemia in early stages

Blood ◽

10.1182/blood.v71.5.1295.1295 ◽

1988 ◽

Vol 71 (5) ◽

pp. 1295-1298 ◽

Cited By ~ 55

Author(s):

C Rozman ◽

E Montserrat ◽

N Vinolas ◽

A Urbano-Ispizua ◽

JM Ribera ◽

...

Keyword(s):

Chronic Lymphocytic Leukemia ◽

Complete Response ◽

Absolute Number ◽

Peripheral Blood Lymphocytes ◽

Response To Therapy ◽

Lymphocytic Leukemia ◽

Hematologic Toxicity ◽

Low Doses ◽

The Absolute ◽

Cell Chronic Lymphocytic Leukemia

Abstract Ten previously untreated patients with early B cell chronic lymphocytic leukemia (B-CLL) (seven in Rai's stage 0, three in stage I) were given recombinant alpha 2-interferon (alpha 2IF) (2 X 10(6) U/m2 intramuscularly three times a week for a minimum of 14 weeks) to assess its effectiveness. All patients were evaluable for response to therapy and toxicity. No complete response was achieved. In all cases a definite, although transient reduction in the absolute number of peripheral blood lymphocytes was observed. In eight patients an increase in the absolute number of granulocytes was detected. None of the patients experienced severe hematologic toxicity. Fatigue, malaise, and fever were the more common side effects, but all patients were able to finish their treatment as planned. The results of this pilot study suggest that low doses of recombinant alpha 2-IF have some activity in early and previously untreated B-CLL and that further studies of IF effectiveness in B-CLL seem warranted.

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Lymphocyte subpopulations and responsiveness in rats bearing intracranial tumors induced by avian sarcoma virus

Journal of Neurosurgery ◽

10.3171/jns.1981.55.4.0554 ◽

1981 ◽

Vol 55 (4) ◽

pp. 554-559 ◽

Cited By ~ 3

Author(s):

Thomas L. Roszman ◽

William H. Brooks ◽

William R. Markesbery ◽

Gary J. Aziz ◽

Darell D. Bigner

Keyword(s):

Concanavalin A ◽

Peripheral Blood ◽

Absolute Number ◽

Peripheral Blood Lymphocytes ◽

Lymphocyte Subpopulations ◽

Avian Sarcoma Virus ◽

Content Type ◽

Blood Lymphocytes ◽

Sarcoma Virus ◽

Avian Sarcoma

✓ The mitogenic responsiveness of peripheral blood lymphocytes obtained from Fischer 344 rats inoculated with avian sarcoma virus was studied. In addition, quantitative alterations in lymphocyte subpopulations were determined in these animals. Only peripheral blood lymphocytes from rats bearing astrocytomas had significantly diminished responses to concanavalin A when compared to control responses. The percentage of lymphocyte subpopulations detected in either the peripheral blood or spleen of tumor-bearing rats did not differ from values obtained with control rats. However, rats bearing astrocytomas had a marked decrease in the absolute number of the various lymphocyte subpopulations as a result of lymphopenia. Neither the sarcoma-bearing rats nor the virus-inoculated rats that did not develop tumors exhibited this lymphopenia. In addition, sera from rats bearing astrocytomas diminished the concanavalin A reactivity of spleen cells obtained from normal rats. The results of this study establish the avian sarcoma virus-induced rat astrocytoma model as a useful immunological parallel for the human disease.

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Levels of Recent Thymic Emigrant Cells Decrease in Children Undergoing Partial Thymectomy during Cardiac Surgery

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.12.5.563-565.2005 ◽

2005 ◽

Vol 12 (5) ◽

pp. 563-565 ◽

Cited By ~ 24

Author(s):

Ashish B. Madhok ◽

A. Chandrasekran ◽

Vincent Parnell ◽

Mysore Gandhi ◽

Devyani Chowdhury ◽

...

Keyword(s):

Cardiac Surgery ◽

T Cell ◽

Postoperative Period ◽

Real Time Pcr ◽

Peripheral Blood Lymphocytes ◽

Fetal Life ◽

Significant Drop ◽

Recent Thymic Emigrant ◽

Cell Pool

ABSTRACT The human thymus is required for establishment of a T-cell pool in fetal life, but postnatal thymectomy is not known to cause immunodeficiency. T-cell emigration from thymus (thymic recent emigrants [TRECs]) is a continuous thymic-dependent process. We studied TREC levels pre- and post-partial thymectomy in children undergoing cardiac surgery. TRECs were quantitated by real-time PCR in peripheral blood lymphocytes of 24 children (0 to 12 years). TREC values were 47916 ± 9271 pre-partial thymectomy and 33157 ± 8479 post-partial thymectomy in 11 paired patients (P = 0.014). Interval between pre- and post-partial thymectomy was 8.8 days ± 5.8 days. Another group of 8 children had 30384 ± 9748 TRECs 16 days to 6 years post-partial thymectomy. There was a significant drop in TREC values post-partial thymectomy in the immediate postoperative period compared to prethymectomy TREC levels. While decreased thymic output may persist, the long-term implications were not evaluated in this patient population.

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IMPROVING THE RESULTS OF TREATMENT OF PATIENTS WITH URETERAL CONCREMENTS AFTER CONTACT URETEROLITHOTRIPSY

Research n Practical Medicine Journal ◽

10.17709/2409-2231-2017-4-2-1 ◽

2017 ◽

Vol 4 (2) ◽

pp. 8-12

Author(s):

R. M. Solh ◽

M. I. Andrukhin ◽

O. V. Makarov ◽

V. V. Fedchenkov

Keyword(s):

Laser Therapy ◽

Postoperative Period ◽

Acute Pyelonephritis ◽

Main Group ◽

Ureteral Stones ◽

Control Group ◽

Results Of Treatment ◽

Group 6 ◽

Beta 2 ◽

Beta 2 Microglobulin

Purpose. Im proving the results of treatment of patients with ureteral stones and reducing the dam aging effects of contact lithotripsy.Materials and methods. In this study, 48 patients were examined aged 20 to 63 years. All patients admitted to the urology department with diagnoses: urolithiasis, calculus of the ureter. In all cases contact ureterolithotripsy with stenting of the upper urinary tract were performed. The patients were divided into two groups: main and control. 25 patients (52 .1%) were included into the main group, which in the postoperative period, we used low-level laser therapy (L-therapy). 23 patients were included in a control group (47.9%) who did not receive low-laser therapy. Laboratory tests and ultrasound with Doppler renal blood vessels scan were performed on admission, on the first day after the operation and on the 5-th day of hospitalization. Low-intensity laser therapy was performed within 5 days after contact ureterolithotripsy on projection of placement of stone and kidney projection by series for 5 minutes.Results. All patients admitted to the hospital, were spared from ureteral stones. In the main group during the treatment with L-therapy a decrease in the level of beta-2 microglobulin to normal was observed. (4.8 ± 0.1 mg/l on the first day. On the 5th day 2 .3 + 0.1 mg/l). In the control group during the treatment without the use of L-therapy, the average level of beta-2 microglobulin decreased but did not reach normal levels. (5.5 ± 0.1 mg/l on the first day. On the 5th day 3,2 ±0, l mg/l).Resistance index in the study group decreased compared to the control. In the control group, cases of acute pyelonephritis were observed. The average hospital stay for patients of the main group (6 days) was less than in the control group (6 .5 + days).Conclusion. The use of laser therapy in the treatment of patients who did undergo ureterolithotripsy can reduce the length of stay of the patient in the hospital and reduce the risk of acute pyelonephritis in the postoperative period. Also it can reduce the damaging effect of lithotripsy on the renal tissues.

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Diagnostics and treatment of abdominal complications after gynecological endoscopic operations

Reproductive health of woman ◽

10.30841/2708-8731.1.2021.229720 ◽

2021 ◽

Vol 1 ◽

pp. 75-79

Author(s):

Yu.P. Vdovichenko ◽

V.I. Boyko ◽

V.A. Terehov

Keyword(s):

Postoperative Complications ◽

Postoperative Period ◽

Diagnostic Laparoscopy ◽

Abdominal Cavity ◽

Early Postoperative Period ◽

Main Group ◽

Laparoscopic Technique ◽

Control Group ◽

Diffuse Peritonitis ◽

Abdominal Complications

The objective: to analyze the results of repeated laparoscopy in gynecological patients with suspected intraperitoneal postoperative complications.Materials and methods. The study involved 42 patients (mean age 46±11,3 years), in whom postoperative abdominal complications after surgical treatment of various gynecological diseases required repeated operations. Depending on the method of re-intervention, the patients were divided into 2 groups: the main group (n=20) – women who only used laparoscopy to correct postoperative complications; control (n=22) – women who were given indications for relaparotomy to correct postoperative complications during diagnostic laparoscopy. Relaparotomy was performed in 8 patients of the control group. All patients underwent a comprehensive examination using clinical, instrumental and laboratory research methods. The groups were homogeneous in terms of age and structure of operations performed on the pelvic organs, and statistically comparable.Results. During the study, the results of repeated laparoscopy of 34 gynecological patients with suspected intraperitoneal postoperative complications were analyzed. In all patients of the main group (47,6%), laparoscopy was revealed as the only and final method of treatment of postoperative complications, while in 7 women repeated relaparoscopy was used from 2 to 4 times, and in 13 women laparoscopy was performed once. In the control group (53,3%), 10 patients during diagnostic laparoscopy were exposed to indications for relaparotomy, in 4 patients the capabilities of the operating team and laparoscopic technique allowed performing some elements of the operation and completing it with a minilaparotomy approach, and 8 patients underwent relaparotomy immediately.Peritonitis and intraperitoneal bleeding prevail in the structure of early abdominal complications. The use of the video laparoscopic technique made it possible in 28,6% of patients with diffuse peritonitis to successfully eliminate the developed complication, eliminate its source and sanitize the abdominal cavity. Laparoscopic hemostasis was successfully performed in 7 patients.Compared with the standard, the use of video endoscopic technique allowed improving the indicators of diagnostic value: to increase the sensitivity level by 1,07 times, specificity by 1,39 times and diagnostic accuracy by 1,11 times.Conclusion. In general, in the early postoperative period, 47 relaparoscopies were performed for suspected complications, which is explained by programmed abdominal sanitization and the use of dynamic laparoscopy to control the course of intraperitoneal complications. Based on the analysis of the material under study, we proposed general indications and contraindications for relaparoscopy in the early postoperative period in order to diagnose and treat postoperative complications. Justifying each of the points, we were guided by the real possibilities of the method in general clinical practice, which in some cases are inferior in their effectiveness to relaparotomy.

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Recombinant alpha 2-interferon in the treatment of B chronic lymphocytic leukemia in early stages

Blood ◽

10.1182/blood.v71.5.1295.bloodjournal7151295 ◽

1988 ◽

Vol 71 (5) ◽

pp. 1295-1298

Author(s):

C Rozman ◽

E Montserrat ◽

N Vinolas ◽

A Urbano-Ispizua ◽

JM Ribera ◽

...

Keyword(s):

Chronic Lymphocytic Leukemia ◽

Complete Response ◽

Absolute Number ◽

Peripheral Blood Lymphocytes ◽

Response To Therapy ◽

Lymphocytic Leukemia ◽

Hematologic Toxicity ◽

Low Doses ◽

The Absolute ◽

Cell Chronic Lymphocytic Leukemia

Ten previously untreated patients with early B cell chronic lymphocytic leukemia (B-CLL) (seven in Rai's stage 0, three in stage I) were given recombinant alpha 2-interferon (alpha 2IF) (2 X 10(6) U/m2 intramuscularly three times a week for a minimum of 14 weeks) to assess its effectiveness. All patients were evaluable for response to therapy and toxicity. No complete response was achieved. In all cases a definite, although transient reduction in the absolute number of peripheral blood lymphocytes was observed. In eight patients an increase in the absolute number of granulocytes was detected. None of the patients experienced severe hematologic toxicity. Fatigue, malaise, and fever were the more common side effects, but all patients were able to finish their treatment as planned. The results of this pilot study suggest that low doses of recombinant alpha 2-IF have some activity in early and previously untreated B-CLL and that further studies of IF effectiveness in B-CLL seem warranted.

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