Association of Single Nucleotide Gene Polymorphism at Interleukin-1β +3954, −511, and −31 in Chronic Periodontitis and Aggressive Periodontitis in Dravidian Ethnicity

Abstract Objectives Periodontitis is a pathological condition of the oral cavity, originating from multiple factors, including microbial, environmental and genetic factors. The vulnerability to several pathologies has been studied with the relationship to genetic polymorphisms, and one of the most prominent is the single nucleotide polymorphisms throughout the genome. The study aimed to find out the association of single nucleotide polymorphism (SNP) of interleukin-1β +3954 gene with chronic periodontitis (CP) in Pakistan Materials and Methods This case–control study was conducted at Dow University of Health Sciences. DNA was extracted from the blood and amplified by using conventional polymerase chain reaction of respective genes followed by sequencing. Mann–Whitney test accessed the difference of clinical parameters between cases and controls, and Fisher’s exact test was applied to access the association of alleles between subjects. Data entered and analyzed using SPSS 21. Results Significant differences were observed in clinical parameters in cases and controls (p < 0.001). In the IL-1β +3954 gene, T alleles were significantly higher in cases as compared with controls (p < 0.001). Genotype CC was significantly dominant in the controls and genotype CT and TT in patients (Chi-square = 19.83, p < 0.001). Conclusion Within the study’s limits, IL-1β +3954 gene polymorphism is associated with periodontitis and is expected to be among the several causes of respective pathology in Pakistan’s population.

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Evaluation of PECAM-1 Gene Polymorphism in Patients with Periodontal Disease and Healthy Individuals

ISRN Dentistry ◽

10.5402/2012/751920 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5

Author(s):

Mahdi Kdkhodazadeh ◽

Mehrdad Hajilooi ◽

Behzad Houshmand ◽

Sara Khazaei ◽

Leila Gholami ◽

...

Keyword(s):

Chronic Periodontitis ◽

Aggressive Periodontitis ◽

Genotype Distribution ◽

Nucleotide Polymorphisms ◽

Healthy Individuals ◽

Single Nucleotide ◽

Genotypic Distribution ◽

Genotype Frequencies ◽

Significant Difference ◽

Polymerase Chain

Objective. Our aim in this paper was to investigate the possible genetic association between three Ser563Asn, Leu125Val and Arg670Gly polymorphisms of the PECAM-1 gene and periodontitis. Methods. Genomic DNA was isolated from whole blood of 105 periodontal patient (52 with chronic periodontitis and 53 with aggressive periodontitis) and 101 healthy individuals. Samples were genotyped and analyzed for the three single-nucleotide polymorphisms (SNPs) of PECAM-1 using polymerase chain reaction with sequence-specific primers (PCR-SSPs). Results. A statistically significant difference was found between the genotypic distribution of the Ser563Asn polymorphism in patients with periodontitis compared to controls (P=0.02). But there were no statistically significant difference between the allele frequencies in the different groups (P=0.05). The other two polymorphisms did not show a statistically significant difference in their allele and genotype frequencies between the groups. There was no statistically significant difference found for any of the polymorphisms allele and genotype distribution in aggressive and chronic periodontitis either. Conclusions. No significant association was found between the polymorphism tested and the subgroups of periodontitis, further research is still necessary to determine whether this polymorphism can be used as a genetic marker of periodontitis.

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Analysis of Interleukin-1β Gene Polymorphism and Its Association with Generalized Aggressive Periodontitis Disease

DNA and Cell Biology ◽

10.1089/dna.2012.1905 ◽

2013 ◽

Vol 32 (7) ◽

pp. 409-413 ◽

Cited By ~ 7

Author(s):

Golnaz Ayazi ◽

Maral Pirayesh ◽

Kheirollah Yari

Keyword(s):

Gene Polymorphism ◽

Aggressive Periodontitis ◽

Interleukin 1Β

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Evaluation of genetic polymorphism of interleukin-1β (rs16944) in chronic and aggressive periodontitis in a group of the Bengali population of West Bengal, India

Journal of Global Oral Health ◽

10.25259/jgoh_45_2020 ◽

2021 ◽

Vol 4 ◽

pp. 27-32

Author(s):

Avishek Das ◽

Ritam Kundu ◽

Ashit Kumar Pal ◽

Somen Bagchi

Keyword(s):

Genetic Polymorphism ◽

Dna Extraction ◽

Chronic Periodontitis ◽

Odds Ratio ◽

West Bengal ◽

Aggressive Periodontitis ◽

Minor Allele ◽

Study Cohort ◽

Single Nucleotide ◽

Chi Square Analysis

Objectives: The genetic basis of periodontitis was demonstrated by formal genetic studies which were focused on a range of various candidate genes selected for their roles in the immune system like genes of the interleukins (ILs) which regulate the intensity of host immunity-inflammatory response. This regulation of host response may be associated with the genetic polymorphisms, specific single-nucleotide polymorphisms of the genes of various ILs. Interleukin-1 (IL-1) is a principal mediator of inflammatory responses acting on many cell types and is itself produced by many different cells, including macrophages, endothelial cells, B cells, fibroblasts, epithelial cells, astrocytes, and osteoblasts in response to microorganisms, bacterial toxins, and complement components. In the present study, an attempt has been made to explore the role of IL-1β-511 (rs16944) genetic polymorphism in chronic as well as aggressive periodontitis in a group of the Bengali population of West Bengal, India. Materials and Methods: A total of 88 Bengali patients of both sexes were recruited in this study and they were divided into three groups: Group A (chronic periodontitis group), Group B (aggressive periodontitis group), and Group C (healthy control). The clinical parameters taken into consideration in the assessment of chronic and aggressive periodontitis were plaque index, calculus index, gingival index, probing pocket depth (PPD), and clinical attachment loss (CAL). A 3 ml of peripheral venous blood was collected from each selected participant and transferred to 3% EDTA containing serum vial and stored at −20°C for DNA extraction. DNA extraction was performed by the phenol chloroform method and ethanol precipitation. Genotyping of extracting DNA samples was carried out for locus IL-1β-511 (rs16944) by real-time polymerase chain reaction. Hardy–Weinberg equilibrium was tested for the gene polymorphism and association between genotypes and cases was examined by odds ratio with 95% confidence interval (CI) and Chi-square analysis using R programming software. Allelic frequencies were calculated according to the number of different alleles observed and the total number of alleles examined. Statistical significance was defined as P < 0.05. Results: On analysis of minor allele frequencies of total periodontitis cases and control, the results were found to be statistically insignificant with P = 0.9799. Minor allele frequency of overall periodontitis cases and controls was 0.405 and 0.403, respectively; the odds ratio was 1.008 and 95% CI ranges from 0.53 to 1.88. Conclusion: The present study suggested no association of single-nucleotide polymorphism of IL-1β-511 (rs16944) with total periodontitis cases (both chronic periodontitis and aggressive periodontitis) in the present study cohort.

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Comparison of CCL28, interleukin-8, interleukin-1β and tumor necrosis factor-alpha in subjects with gingivitis, chronic periodontitis and generalized aggressive periodontitis

Journal of Periodontal Research ◽

10.1111/j.1600-0765.2012.01500.x ◽

2012 ◽

Vol 48 (1) ◽

pp. 44-51 ◽

Cited By ~ 66

Author(s):

A. S. Ertugrul ◽

H. Sahin ◽

A. Dikilitas ◽

N. Alpaslan ◽

A. Bozoglan

Keyword(s):

Tumor Necrosis Factor ◽

Tumor Necrosis Factor Alpha ◽

Chronic Periodontitis ◽

Tumor Necrosis ◽

Aggressive Periodontitis ◽

Interleukin 8 ◽

Interleukin 1Β ◽

Necrosis Factor Alpha ◽

Factor Alpha ◽

Necrosis Factor

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Single nucleotide polymorphisms associated with aggressive periodontitis and severe chronic periodontitis in Japanese

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2004.03.126 ◽

2004 ◽

Vol 317 (3) ◽

pp. 887-892 ◽

Cited By ~ 37

Author(s):

Asami Suzuki ◽

Guijin Ji ◽

Yukihiro Numabe ◽

Masaaki Muramatsu ◽

Kazuhiro Gomi ◽

...

Keyword(s):

Single Nucleotide Polymorphisms ◽

Chronic Periodontitis ◽

Aggressive Periodontitis ◽

Nucleotide Polymorphisms ◽

Single Nucleotide

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Association between IFN-γ+874A/T and IFN-γR1 (-611A/G, +189T/G, and +95C/T) Gene Polymorphisms and Chronic Periodontitis in a Sample of Iranian Population

International Journal of Dentistry ◽

10.1155/2015/375359 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 12

Author(s):

Zahra Heidari ◽

Hamidreza Mahmoudzadeh-Sagheb ◽

Mohammad Hashemi ◽

Somayeh Ansarimoghaddam ◽

Bita Moudi ◽

...

Keyword(s):

Gene Polymorphism ◽

Genetic Polymorphisms ◽

Chronic Periodontitis ◽

Gene Polymorphisms ◽

Healthy Controls ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Significant Difference ◽

Pcr Rflp ◽

And Control

Background. Interferon gamma (IFN-γ) is an immune regulatory cytokine that acts through its receptor and plays important role in progression of inflammatory disease such as chronic periodontitis (CP). The purpose of this study was to determine the differences in the distribution of IFN-γ(+874A/T) and IFN-γR1 (-611A/G, +189T/G, and +95C/T) gene polymorphisms among CP and healthy individuals and to investigate relationships between these polymorphisms and susceptibility to CP.Materials and Methods. 310 individuals were enrolled in the study including 210 CP patients and 100 healthy controls. Single nucleotide polymorphisms at IFN-γ(+874A/T) and IFN-γR1 (-611A/G, +189T/G, and +95C/T) were analyzed by ARMS-PCR and PCR-RFLP methods.Results. The significant difference was found in genotype and allele frequency of IFN-γ(+874A/T) gene polymorphism in chronic periodontitis patients and healthy controls. The distribution of genotypes and allele frequencies for IFN-γR1 (-611A/G, +189T/G, and +95C/T) were similar among the groups and no differences in the frequencies of alleles or genotypes of IFN-γR1 genetic polymorphisms variants between case and control groups were detected.Conclusion.The finding of this study showed that IFN-γ+874A/T gene polymorphism may affect susceptibility to CP, whereas IFN-γR1 genetic polymorphisms at -611A/G, +189T/G, and +95C/T were not associated with this disease.

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Genetic Variation of Myeloperoxidase Gene Contributes to Aggressive Periodontitis: A Preliminary Association Study in Turkish Population

Disease Markers ◽

10.1155/2010/734619 ◽

2010 ◽

Vol 28 (2) ◽

pp. 95-99 ◽

Cited By ~ 4

Author(s):

Kamile Erciyas ◽

Sacide Pehlivan ◽

Tugce Sever ◽

Recep Orbak

Keyword(s):

Gene Polymorphism ◽

Allele Frequency ◽

Chronic Periodontitis ◽

Turkish Population ◽

Aggressive Periodontitis ◽

Genotype Frequency ◽

Tissue Destruction ◽

Clinical Attachment Loss ◽

Probing Depth ◽

Increased Risk

Myeloperoxidase (MPO) is a lysosomal enzyme found in the azurophilic granules of polymorphonuclear leukocytes. It is involved in the defense against periodontal bacteria, and is also able to mediate inflammatory tissue destruction in aggressive and chronic periodontitis. The aim of this study was to explore the association between MPO-463G/A gene polymorphism and aggressive periodontitis (AgP) and chronic periodontitis (CP). The study included 147 subjects. Probing depth (PD), clinical attachment loss (CAL), plaque index (PI), and gingival index (GI) were recorded as the clinical parameters. Genomic DNA was obtained from the peripheral blood of 32 subjects with AgP, 25 with CP, and 90 reference controls. We genotyped the MPO-463G/A polymorphism using the PCR-RFLP method. All data were analyzed using SPSS version 13.0 for windows. There were no significant differences between the CP patients and controls regarding MPO-463A/G gene polymorphism either in terms of allele frequency or genotype frequency of MPO-463A/G. However, either in terms of allele frequency or genotype frequency of MPO-463A/G, there were significant differences between the AgP patients and the controls. In conclusion, our data suggest that MPO-463G/A may be associated with increased risk of aggressive periodontitis in Turkish patients.

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