scholarly journals Assessment of the biodegradation of doxycycline by biostimulation with addition of glucose, phenol or/and copper

Ecocycles ◽  
2020 ◽  
Vol 6 (2) ◽  
pp. 25-31
Author(s):  
Hayet Djelal ◽  
Paola Estrada Martinez ◽  
Djouza Haddouche ◽  
Malika Chabani
Keyword(s):  
Veterinary Medicine ◽  
Pharmaceutical Industry ◽  
Activated Sludge ◽  
Trametes Versicolor ◽  
Conventional Treatment ◽  
Microbial Population ◽  
White Rot Fungi ◽  
White Rot ◽  
Laccase Production ◽  
Biotic Control

Doxycycline, an antibiotic, is largely used in human and veterinary medicine. The conventional treatment with activated sludge is not very efficient. Laccase appeared to be the main enzyme secreted essentially by white rot fungi as Trametes versicolor and Phlebia fascicularia on the degradation of xenobiotic compounds from the pharmaceutical industry. The main purpose of this study was to enhance the biodegradation of doxycycline through activated sludge combined with addition of glucose as a carbon co-substrate to improve the growth of the microbial population present in the activated sludge, phenol as a laccase mediator, copper as a cofactor and inductor for laccase production. The enhancement of the biodegradation of doxycycline was 50, 90, 68 and 83% greater respectively with the addition of glucose, copper, phenol and with a mixture of the compounds after 14 days of treatment at 25°C. Compared with the biotic control (activated sludge alone), a 30% increase for the test with the addition of phenol was observed.

scholarly journals Mechanism of Laccase Induction via Emodin in Trametes versicolor

2021 ◽  
Vol 9 ◽  
Author(s):  
Lin Wang ◽  
Xuecai Luo ◽  
Yu Pan ◽  
Zai Zheng ◽  
Ruochun Yin ◽  
...  
Keyword(s):  
Trametes Versicolor ◽  
Large Scale ◽  
White Rot Fungi ◽  
Antioxidant Response ◽  
Chinese Herbs ◽  
White Rot ◽  
Laccase Production ◽  
Related Factor ◽  
Response Element ◽  
Laccase Genes

Secondary metabolites of traditional Chinese herbs can prominently stimulate the production of laccase from white rot fungi during submerged fermentation. However, the molecular mechanism through which these natural products induce the production of laccase remains unknown. In this study, the Chinese herbal medicine Polygonum cuspidatum was used to induce laccase production in Trametes versicolor, and the best inducer was identified in emodin, even under conditions of 1000-L, large-scale fermentation. Proteomics analysis identified a selection of proteins that were differentially expressed in the presence of emodin, indicating that emodin may affect the expression of laccase genes through three mechanisms: reducing bioenergy productivity, the aryl hydrocarbon receptor (AHR)/xenobiotic response element (XRE) pathway, and the nuclear erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) pathway. Combined with protoplast flow cytometry and fluorescence, it is revealed that emodin might reduce the synthesis of ATP by lowering the mitochondrial membrane potential, leading to the subsequent responses.

scholarly journals A Novel Approach in Crude Enzyme Laccase Production and Application in Emerging Contaminant Bioremediation

Processes ◽  
2020 ◽  
Vol 8 (6) ◽  
pp. 648
Author(s):  
Luong N. Nguyen ◽  
Minh T. Vu ◽  
Md Abu Hasan Johir ◽  
Nirenkumar Pathak ◽  
Jakub Zdarta ◽  
...  
Keyword(s):  
Steroid Hormones ◽  
Liquid Culture ◽  
Emerging Contaminants ◽  
White Rot Fungi ◽  
Enzyme Concentration ◽  
Crude Enzyme ◽  
White Rot ◽  
Laccase Production ◽  
Solid Culture ◽  
Novel Approach

Laccase enzyme from white-rot fungi is a potential biocatalyst for the oxidation of emerging contaminants (ECs), such as pesticides, pharmaceuticals and steroid hormones. This study aims to develop a three-step platform to treat ECs: (i) enzyme production, (ii) enzyme concentration and (iii) enzyme application. In the first step, solid culture and liquid culture were compared. The solid culture produced significantly more laccase than the liquid culture (447 vs. 74 µM/min after eight days), demonstrating that white rot fungi thrived on a solid medium. In the second step, the enzyme was concentrated 6.6 times using an ultrafiltration (UF) process, resulting in laccase activity of 2980 µM/min. No enzymatic loss due to filtration and membrane adsorption was observed, suggesting the feasibility of the UF membrane for enzyme concentration. In the third step, concentrated crude enzyme was applied in an enzymatic membrane reactor (EMR) to remove a diverse set of ECs (31 compounds in six groups). The EMR effectively removed of steroid hormones, phytoestrogen, ultraviolet (UV) filters and industrial chemical (above 90%). However, it had low removal of pesticides and pharmaceuticals.

scholarly journals Enhanced Production of Ligninolytic Enzymes by a Mushroom Stereum ostrea

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
K. Y. Usha ◽  
K. Praveen ◽  
B. Rajasekhar Reddy
Keyword(s):  
Solid State ◽  
Copper Sulphate ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Tween 80 ◽  
Veratryl Alcohol ◽  
White Rot ◽  
Tween 20 ◽  
Laccase Production ◽  
Enhanced Production

The white rot fungi Stereum ostrea displayed a wide diversity in their response to supplemented inducers, surfactants, and copper sulphate in solid state fermentation. Among the inducers tested, 0.02% veratryl alcohol increased the ligninolytic enzyme production to a significant extent. The addition of copper sulphate at 300 μM concentration has a positive effect on laccase production increasing its activity by 2 times compared to control. Among the surfactants, Tween 20, Tween 80, and Triton X 100, tested in the studies, Tween 80 stimulated the production of ligninolytic enzymes. Biosorption of dyes was carried out by using two lignocellulosic wastes, rice bran and wheat bran, in 50 ppm of remazol brilliant blue and remazol brilliant violet 5R dyes. These dye adsorbed lignocelluloses were then utilized for the production of ligninolytic enzymes in solid state mode. The two dye adsorbed lignocelluloses enhanced the production of laccase and manganese peroxidase but not lignin peroxidase.

Ability of white-rot fungi to remove selected pharmaceuticals and identification of degradation products of ibuprofen by Trametes versicolor

Chemosphere ◽  
2009 ◽  
Vol 74 (6) ◽  
pp. 765-772 ◽  
Author(s):  
Ernest Marco-Urrea ◽  
Miriam Pérez-Trujillo ◽  
Teresa Vicent ◽  
Gloria Caminal
Keyword(s):  
Trametes Versicolor ◽  
Degradation Products ◽  
White Rot Fungi ◽  
White Rot

scholarly journals Comparative study on laccase activity of white rot fungi under submerged fermentation with different lignocellulosic wastes

BioResources ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. 9166-9179
Author(s):  
Qi An ◽  
Jie Qiao ◽  
Lu-Sen Bian ◽  
Mei-Ling Han ◽  
Xun-You Yan ◽  
...  
Keyword(s):  
Submerged Fermentation ◽  
Laccase Activity ◽  
White Rot Fungi ◽  
Industrial Applications ◽  
Culture Conditions ◽  
White Rot ◽  
Laccase Production ◽  
Poplar Wood ◽  
Lignocellulosic Wastes ◽  
Cottonseed Hull

Different Pleurotus ostreatus and Flammulina velutipes species were compared relative to their ability to produce laccase in submerged fermentation of various lignocellulosic wastes. Fungi cultivation in identical culture conditions revealed wide differences among both species and strains of the same species. The laccase secretion ability of P. ostreatus strains was superior to F. velutipes strains. Maximum laccase production on cottonseed hull, corncob, and poplar wood was secreted by P. ostreatus CY 568, P. ostreatus CCEF 89, and P. ostreatus CY 568, respectively. The nature of lignocellulosic materials played an important role in determining the expression of laccase potential of fungi. The presence of cottonseed hull improved laccase activity and accelerated the rate of enzyme production. Maximum laccase production on cottonseed hull was nearly 1.29-fold and 1.53-fold higher than that on corncob and poplar wood, respectively. Laccase activity was detected in almost all tested strains on cottonseed hull on the first day, while only a few strains on poplar wood and corncob were detected on the first day. These findings will be helpful for selecting the appropriate strain in industrial applications and for optimization of integrated industrial laccase production.

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