scholarly journals Putative periodontal bacteria in clinically healthy and diseased sites of periodontitis patients

2019 ◽  
Vol 18 ◽  
pp. e191417
Author(s):  
Carlos Eduardo Barros ◽  
Vivian Siqueira ◽  
Dennis Carvalho ◽  
Antonio Canabarro
Keyword(s):  
Dna Hybridization ◽  
Healthy Subjects ◽  
Bacterial Species ◽  
Treponema Denticola ◽  
Hybridization Technique ◽  
Pocket Depth ◽  
Microbial Profile ◽  
Probing Pocket Depth ◽  
Periodontal Bacteria ◽  
Microbial Analysis

Aim: The aim of this study was to compare the microbial profile of subgingival sites in Periodontitis (Pd) patients and healthy ones. Methods: Eighteen patients with Pd and 18 gender-matched healthy controls were selected. Subgingival samples were collected from three types of sites: 1) healthy site of healthy subjects (probing pocket depth (PPD) ≤ 3mm, CG), 2) healthy site of Pd patients (PPD ≤ 3mm, PG-C) and 3) diseased site (PPD > 3mm) of the same Pd patients (PG-T). All sites were subjected to microbial analysis for the detection of 40 bacterial species by the "Checkerboard DNA-DNA hybridization" technique. Results: It was observed a great diversity of bacteria in all patients evaluated. The sites from the Pd groups (PG-T and PG-C) showed a higher overall count of the studied bacteria than those of the CG group, especially from Green, Orange, and Red complexes. Also, PG-T showed a higher prevalence of Red complex bacteria than CG. Individual pathogens, such as Porphyromonas gingivalis, Treponema denticola and Treponema socranskii were detected in higher levels and/or prevalence in Pd than in control patients. However, it was not observed any difference between PG-T and PG-C. Conclusion: Pd patients showed higher prevalence and counts of some putative periodontal bacteria, especially from the red complex, than control ones, regardless of the severity of their sites.

scholarly journals Title IgA Nephropathy and Oral Bacterial Species Related to Dental Caries and Periodontitis

2022 ◽  
Vol 23 (2) ◽  
pp. 725
Author(s):  
Yasuyuki Nagasawa ◽  
Taro Misaki ◽  
Seigo Ito ◽  
Shuhei Naka ◽  
Kaoruko Wato ◽  
...  
Keyword(s):  
Dental Caries ◽  
Bacterial Infection ◽  
Iga Nephropathy ◽  
Bacterial Infections ◽  
Bacterial Species ◽  
Oral Bacteria ◽  
Treponema Denticola ◽  
Collagen Binding ◽  
Periodontal Bacteria ◽  
The Relationship

A relationship between IgA nephropathy (IgAN) and bacterial infection has been suspected. As IgAN is a chronic disease, bacteria that could cause chronic infection in oral areas might be pathogenetic bacteria candidates. Oral bacterial species related to dental caries and periodontitis should be candidates because these bacteria are well known to be pathogenic in chronic dental disease. Recently, several reports have indicated that collagen-binding protein (cnm)-(+) Streptococcs mutans is relate to the incidence of IgAN and the progression of IgAN. Among periodontal bacteria, Treponema denticola, Porphyromonas gingivalis and Campylobacte rectus were found to be related to the incidence of IgAN. These bacteria can cause IgAN-like histological findings in animal models. While the connection between oral bacterial infection, such as infection with S. mutans and periodontal bacteria, and the incidence of IgAN remains unclear, these bacterial infections might cause aberrantly glycosylated IgA1 in nasopharynx-associated lymphoid tissue, which has been reported to cause IgA deposition in mesangial areas in glomeruli, probably through the alteration of microRNAs related to the expression of glycosylation enzymes. The roles of other factors related to the incidence and progression of IgA, such as genes and cigarette smoking, can also be explained from the perspective of the relationship between these factors and oral bacteria. This review summarizes the relationship between IgAN and oral bacteria, such as cnm-(+) S. mutans and periodontal bacteria.

Periodontal Bacterial Load: A Proposed New Epidemiological Method for Periodontal Disease Assessment

2010 ◽  
Vol 11 (1) ◽  
pp. 49-56
Author(s):  
Magda Feres ◽  
Luciene Cristina de Figueiredo ◽  
Mario Vianna Vettore ◽  
Anna Thereza Thomé Leão ◽  
Maria do Carmo Leal ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Bacterial Species ◽  
Bacterial Load ◽  
Disease Assessment ◽  
Postpartum Women ◽  
Hybridization Technique ◽  
Pocket Depth ◽  
Periodontal Status ◽  
Epidemiological Method ◽  
Attachment Level

Abstract Aim The purpose of this study was to identify a periodontal clinical measure that correlates with red complex bacteria usually associated with periodontal disease. Methods and Materials Periodontal clinical parameters were recorded in 116 postpartum women at six sites per tooth for all teeth excluding third molars. Two subgingival plaque samples per subject were collected and analyzed for 39 bacterial species using the Checkerboard DNADNA hybridization technique. Periodontal Bacterial Load (PBL) was calculated as the sum of all pocket depth measurements of 4 mm at sites with a Clinical Attachment Level (CAL) of 4 mm. The association of clinical and bacterial scores was analyzed using the Spearman correlation coefficient and the Kruskal-Wallis test. Results The PBL was correlated with microorganisms from the red complex that included Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola, individually or grouped (p<0.05). The PBL was not associated with periodontally beneficial species from the yellow, green, purple, and blue complexes (p>0.05). The proportions and mean counts of the red complex were increased according to the quartile groups of distribution of the PBL. Conclusions PBL appears to be a reliable measure of periodontal status in postpartum women. Clinical Significance PBL avoids bias in the assessment of periodontal status in studies of periodontal disease. Citation Vettore MV, Leão ATT, Leal MC, Feres M, Figueiredo LC, Sheiham A. Periodontal Bacterial Load: A Proposed New Epidemiological Method for Periodontal Disease Assessment. J Contemp Dent Pract [Internet]. 2010 Jan; 11(1):049-056. Available from: http://www.thejcdp. com/journal/view/volume11-issue1-vettore.

Effect of sample storage time on detection of hybridization signals in Checkerboard DNA–DNA hybridization

2012 ◽  
Vol 58 (4) ◽  
pp. 502-506 ◽  
Author(s):  
Cássio do Nascimento ◽  
Katia Muller ◽  
Sandra Sato ◽  
Rubens Ferreira Albuquerque Junior
Keyword(s):  
Dna Hybridization ◽  
Healthy Subjects ◽  
Storage Time ◽  
Bacterial Species ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Hybridization Method ◽  
Sample Storage ◽  
Storage Times

Long-term sample storage can affect the intensity of the hybridization signals provided by molecular diagnostic methods that use chemiluminescent detection. The aim of this study was to evaluate the effect of different storage times on the hybridization signals of 13 bacterial species detected by the Checkerboard DNA–DNA hybridization method using whole-genomic DNA probes. Ninety-six subgingival biofilm samples were collected from 36 healthy subjects, and the intensity of hybridization signals was evaluated at 4 different time periods: (1) immediately after collecting (n = 24) and (2) after storage at –20 °C for 6 months (n = 24), (3) for 12 months (n = 24), and (4) for 24 months (n = 24). The intensity of hybridization signals obtained from groups 1 and 2 were significantly higher than in the other groups (p < 0.001). No differences were found between groups 1 and 2 (p > 0.05). The Checkerboard DNA–DNA hybridization method was suitable to detect hybridization signals from all groups evaluated, and the intensity of signals decreased significantly after long periods of sample storage.

scholarly journals Microbial Profile and Endotoxin Levels in Primary Periodontal Lesions with Secondary Endodontic Involvement

2019 ◽  
Vol 30 (4) ◽  
pp. 356-362
Author(s):  
Emanuel da Silva Rovai ◽  
Felipe de Souza Matos ◽  
Warley David Kerbauy ◽  
Flávia Goulart da Rosa Cardoso ◽  
Frederico Canato Martinho ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Root Canal ◽  
Bacterial Species ◽  
Wilcoxon Test ◽  
Periodontal Pocket ◽  
Strongly Correlated ◽  
Exact Test ◽  
Root Canals ◽  
Microbial Profile ◽  
Lal Assay

Abstract This study was carried out to investigate the microbial profile and endotoxin levels of endodontic-periodontal lesions of periodontal origin. Periodontal and endodontic samples were taken from periodontal pockets and necrotic root canals of 10 teeth with endodontic-periodontal lesions. Evidencing of 40 different bacterial species were determined in each endodontic and periodontal sample using the checkerboard DNA-DNA hybridization method and Kinetic chromogenic LAL assay was used for quantification of endotoxins. Fisher’s exact test correlated the bacterial species with the endodontic or periodontal microbiota. The endotoxin levels (EU/mL) found in samples of the root canal and periodontal pocket were compared by the Wilcoxon test (p<0.05). Bacteria and LPS units were found in 100% of the endodontic and periodontal samples. The species E. faecium, P. acnes, G. morbillorum, C. sputigena and L. buccalis were strongly correlated with the endodontic microbiota and P. nigrescens with the periodontal microbiota. P. intermedia, P. endodontalis and V. parvula were more prevalent in both endodontic and periodontal microbiots. The endotoxin levels in the periodontal pocket (89600 EU/mL) were significantly higher than in the root canal (2310 EU/mL). It was concluded that the microbiota present in the periodontal and endodontic tissues is similar, with a higher prevalence of species of the orange complex and a higher level of endotoxin in the periodontal pockets.

scholarly journals Origin of MMP-8 and Lactoferrin levels from gingival crevicular fluid, salivary glands and whole saliva

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Liza L. Ramenzoni ◽  
Deborah Hofer ◽  
Alex Solderer ◽  
Daniel Wiedemeier ◽  
Thomas Attin ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Salivary Glands ◽  
Gingival Crevicular Fluid ◽  
Clinical Parameters ◽  
Pocket Depth ◽  
Active Matrix ◽  
Probing Pocket Depth ◽  
Whole Saliva ◽  
Biomarker Testing ◽  
Crevicular Fluid

Abstract Background Pathologically elevated levels of matrix metalloproteinase-8 (MMP-8) and Lactoferrin in oral fluids have been associated with the presence of gingivitis/periodontitis. This study aimed to assess the origin of MMP-8 and Lactoferrin in periodontitis patients and to identify the degree to which conventional clinical parameters correlate with their presence. Methods A total of ten periodontitis and ten healthy patients were included in this study. Whole saliva (stimulated and unstimulated), parotid/sublingual glandular fluid and gingival crevicular fluid from pockets and sulci were tested for MMP-8 and Lactoferrin and protein concentrations were quantified using an ELISA assay. Clinical parameters were checked for potential associations with MMP-8 and Lactoferrin levels. Results Periodontal patients presented higher concentrations of MMP-8 and Lactoferrin in pockets than other sources (P = 0.03). Lactoferrin measurement was higher in the parotid compared to sublingual glandular fluid in periodontitis patients (P = 0.03). Increased probing pocket depth was positively correlated with high MMP-8 and Lactoferrin levels. Conclusions Periodontal pockets appear to be the major source of active matrix metalloproteinase and Lactoferrin, which also may also enter the oral cavity through the salivary glands. Since clinically healthy sites in periodontitis patients also had elevated biomarker levels, gingival crevicular fluid biomarker testing may be more predictive of future tissue breakdown than conventional clinical parameters.

Association of ALDH2 Genotypes with Periodontitis Progression

2009 ◽  
Vol 89 (2) ◽  
pp. 138-142 ◽  
Author(s):  
N. Nishida ◽  
M. Tanaka ◽  
S. Sekine ◽  
T. Takeshita ◽  
K. Nakayama ◽  
...  
Keyword(s):  
Alcohol Consumption ◽  
Periodontal Disease ◽  
Disease Progression ◽  
Odds Ratio ◽  
Alcohol Sensitivity ◽  
Pocket Depth ◽  
Probing Pocket Depth ◽  
Significant Odds Ratio ◽  
Progression Risk ◽  
Prospective Association

The progression of periodontitis may be affected by ALDH2 genotypes with respect to the oxidation of acetaldehyde to acetate, which leads to the accumulation of acetaldehyde in plasma and potential toxic effects. We examined the prospective association of ALDH2 genotypes in terms of alcohol sensitivity between alcohol consumption and periodontal disease progression. In 2003, 224 of 256 (87.5%) individuals examined at baseline (1999) completed probing pocket depth measurements for the evaluation of periodontitis progression. Missing data on self-reported questionnaires and blood samples were excluded; therefore, 183 samples were analyzed. Individuals who consumed ≥ 33.0 g/day of alcohol exhibited high periodontal disease progression risk (OR = 3.54). ALDH2 *1/*2 individuals who consumed ≥ 33 g/day of alcohol displayed a significant odds ratio (OR = 4.28) of periodontitis progression risk, in contrast to ALDH2 *1/*1 individuals. These results suggested that alcohol consumption as well as alcohol sensitivity may be a risk factor for periodontitis progression.

Peptoniphilus Colimassiliensis sp. nov. and Peptoniphilus Urinimassiliensis sp. nov., Two New Species Isolated From Humans

2021 ◽  
Author(s):  
Babacar Mbaye ◽  
Cheikh Ibrahima LO ◽  
Niokhor Dione ◽  
Sarah Benabdelkader ◽  
Maryam Tidjani Alou ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dna Hybridization ◽  
Kidney Transplant Recipient ◽  
Bacterial Species ◽  
Hexadecanoic Acid ◽  
Anaerobic Conditions ◽  
New Members ◽  
Two New Species ◽  
Type Strains ◽  
Gram Positive Cocci

Abstract Strains Marseille-P3761 and Marseille-P3195 are representatives of two bacterial species isolated from human specimens. Strain Marseille-P3761 was isolated from the stool of a healthy volunteer, while strain Marseille-P3915 was cultivated from the urine of a kidney transplant recipient. Both strains are anaerobic Gram-positive cocci bacteria. Both are catalase-negative and oxidase-negative and grow optimally at 37°C in anaerobic conditions. They also metabolize carbohydrates such as galactose, glucose, fructose, and glycerol. The major fatty acids were hexadecanoic acid for both strains, Marseille-P3761 (38%) and Marseille-P3195 (31%). The highest DNA-DNA hybridization values of Marseille-P3761 and Marseille-P3195 strains when compared to their closest phylogenetic relatives were 52.3% and 56.4%, respectively. The morphological, biochemical, phenotypic and genomic characteristics strongly support that these strains are new members of the Peptoniphilus genus. Thus, we suggest that strains Marseille-P3761 (CSUR P3761 = CCUG71569) and Marseille-P3195 (CSUR P3195 = DSM 103468) are the type strains of two new Peptoniphilus species, for which we propose the names Peptoniphilus colimassiliensis sp. nov. and Peptoniphilus urinimassiliensis sp. nov., respectively.

scholarly journals Phenytoin-Induced Gingival Overgrowth Management with Periodontal Treatment

2015 ◽  
Vol 26 (1) ◽  
pp. 39-43 ◽  
Author(s):  
Bruno César de Vasconcelos Gurgel ◽  
Carlos Roberto Batista de Morais ◽  
Pedro Carlos da Rocha-Neto ◽  
Euler Maciel Dantas ◽  
Leão Pereira Pinto ◽  
...  
Keyword(s):  
Oral Hygiene ◽  
Dental Treatment ◽  
Treatment Plan ◽  
Pocket Depth ◽  
Gingival Overgrowth ◽  
Probing Pocket Depth ◽  
Supportive Periodontal Therapy ◽  
Continuous Use ◽  
Oral Hygiene Instruction

Phenytoin-induced gingival overgrowth (PIGO) is a common complication of the continuous use of medications. This paper presents a case of PIGO hindering oral function and compromising oral hygiene and aesthetics, which was treated with a combination of nonsurgical and surgical periodontal therapies. A 39-year-old male patient was referred for dental treatment with several complaints, especially upper and lower gingival overgrowth that hindered speech and swallowing. Generalized deep probing pockets and bone loss were detected. Diagnosis of gingival overgrowth associated with phenytoin and chronic periodontitis was established. The treatment plan consisted of conservative therapy with education on oral health, motivation and meticulous oral hygiene instruction in combination with scaling and root planing. During the revaluation period, a marked reduction in the clinical parameters was noted, particularly probing pocket depth reduction. Surgical therapy for removal of gingival overgrowth was also performed to achieve pocket reduction. Supportive periodontal therapy was proposed and the patient is currently under follow-up for 4 years. Management of PIGO may be obtained by the use of periodontal procedures combined with good oral hygiene and periodontal supportive care.

Clinical and Patient-Related Outcomes of a Tapered Implant System With Switched Platform Conical Abutments: A Private Practice Field Trial

2018 ◽  
Vol 44 (5) ◽  
pp. 326-329
Author(s):  
Jacob Horwitz ◽  
Eli E. Machtei ◽  
Shai Frankental ◽  
Eran Gabay ◽  
Yaniv Mayer ◽  
...  
Keyword(s):  
Dental Implants ◽  
Field Trial ◽  
Subjective Evaluation ◽  
Success Rates ◽  
Pocket Depth ◽  
Probing Pocket Depth ◽  
Satisfaction Evaluation ◽  
Edentulous Patients ◽  
Standard Implant ◽  
Implant System

The aim of this prospective cohort observational field trial was to examine 1-year survival and success rates of a recently introduced tapered implant system with switched platform conical abutments and to evaluate patient related outcomes of therapy. Partially edentulous patients aged between 18 and 75 years, with available bone height for dental implants ≥10 mm desiring to restore the missing tooth/teeth with implant supported restoration, were recruited by 7 periodontists in their respective private practices. Dental implants were installed according to standard implant therapy protocol. Three to 6 months postoperatively, after evaluating interim implant success, implants were restored by the referring dentists. Patient, Ramfjord teeth, and implant data, including baseline and 1-year postoperative, were collected. A total of 60 patients were recruited and received 117 implants. Complete 1-year clinical and radiographic data were available for 83 and 65 implants, respectively. Two implants failed during the first year, resulting in a 1-year survival rate of 98.3%. Mean implant probing pocket depth was 2.29 ± 0.84 mm. Mean radiographic bone distance from implant's shoulder at the mesial and distal sites at 1 year was 0.66 ± 0.5 and 0.79 ± 0.64mm, respectively, resulting in a success rate of 95.4%. Patient subjective evaluation of therapy exhibited a median pain experience of 1 and median esthetics, function, and general satisfaction evaluation of 10 on a scale of 1 to 10. The tapered conical connection dental implant system, used in private dental practices, shows good 1-year survival and success rates that are similar to other implant systems on the market.

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