scholarly journals Optimasi Kondisi Fermentasi pada Produksi Metabolit Antibakteri dari Bacillus tequilensis BSMF Simbiotik Halichondria panicea

2021 ◽  
Vol 8 (2) ◽  
pp. 187
Author(s):  
Nindya Pramesti Wardani ◽  
Achmad Toto Poernomo ◽  
Isnaeni Isnaeni
Keyword(s):  
Staphylococcus Aureus ◽  
Potato Dextrose Agar ◽  
Halichondria Panicea ◽  
Bacillus Tequilensis

Pendahuluan: Resistensi antibakteri merupakan masalah kesehatan global yang dialami hampir di seluruh negara. Eksplorasi antibakteri dari sumber baru seperti tumbuhan, hewan, dan mikroorganisme baik yang hidup bebas maupun bersimbiosis menjadi solusi alternatif untuk mengatasi resistensi antibakteri. Mikroorganisme berupa bakteri ditargetkan sebagai sumber antibakteri yang berkelanjutan karena jumlahnya melimpah dan mudah dalam proses pembiakan. Bakteri yang hidup bersimbiosis diketahui dapat memproduksi metabolit antibakteri berspektrum lebih luas dibandingkan bakteri yang hidup bebas. Bakteri dapat bersimbiosis dengan berbagai makhluk hidup termasuk organisme multiseluler seperti spons. Isolat Bacillus tequilensis BSMF yang bersimbiosis dengan Halichondria panicea dari Perairan Cabbiya Madura menunjukkan adanya produksi metabolit yang memiliki aktivitas antibakteri. Tujuan: Menentukan pH dan suhu optimum untuk produksi metabolit antibakteri dari Bacillus tequilensis BSMF simbiotik Halichondria panicea. Metode: Produksi metabolit antibakteri dilakukan dengan metode fermentasi padat pada media Potato Dextrose Agar (PDA) yang telah diatur pH dan suhu inkubasinya, sedangkan uji aktivitas antibakteri terhadap Staphylococcus aureus ATCC 25923 dan Eschericia coli ATCC 25922 dilakukan menggunakan metode difusi agar. Penentuan aktivitas antibakteri dilakukan melalui pengukuran diameter zona hambat. Hasil: pH media yang menunjukkan aktivitas antibakteri optimum Bacillus tequilensis BSMF terhadap Staphylococcus aureus ATCC 25923 dan Eschericia coli ATCC 25922 adalah 8 ± 0,5 pada suhu inkubasi 32 ± 1oC dengan rata- rata indeks aktivitas antibakteri berturut- turut 2,74 ± 0,07 dan 3,39 ± 0,07. Kesimpulan: pH dan suhu optimum yang diperoleh adalah pH 8 ± 0,5 dan suhu 32 ± 1oC.

Concentration of respirable dust and bioaerosols and identification of certain microbial types in a hog-growing facility

1991 ◽  
Vol 71 (2) ◽  
pp. 271-277 ◽  
Author(s):  
M. Butera ◽  
J. H. Smith ◽  
W. D. Morrison ◽  
R. R. Hacker ◽  
F. A. Kains ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Particle Size ◽  
Light Scattering ◽  
Relative Humidity ◽  
Ventilation Rate ◽  
Potato Dextrose Agar ◽  
Respirable Dust ◽  
Bacterial Isolates ◽  
Gram Positive Bacteria ◽  
Andersen Sampler

In order to assess the effects of ventilation rate, temperature, relative humidity and source of air on bioaerosol levels and dust with particle size < 10 μm, a total of 120 pigs housed in 12 pens in two separate rooms were used. Pigs averaged 30 kg initially and the trials were discontinued when 20% of the pigs were marketed. A six-stage Andersen sampler and a light scattering particle counter were used to determine bioaerosols and respirable dust (0.1–10 μm), respectively. Total bioaerosols were assessed using Trypticase Soy Agar. Potato Dextrose Agar was used for fungal aeorsols and Baird-Parker Agar used for isolation of Staphylococcus aureus. Moulds amounted to less than 1% of total microorganisms. Gram positive bacteria made up 72% of the bacterial isolates. Respirable dust was not correlated with respirable bioaerosols. Ventilation rate (2, 5 or 8 changes h−1) did not affect bioaerosol level or respirable dust. Total bioaerosols were significantly reduced (P < 0.05) in higher temperatures only. Relative humidity did not influence total bioaerosols but in one series respirable bioaerosols were significantly (R = 0.53) (P < 0.05) correlated with RH. Total bioaerosols were not different in outside air or attic air. Key words: Dust, bioaerosols, pigs, ventilation

scholarly journals Uji Efek Antibakteri Jamur Endofit Akar Bakau Rhizophora stylosa Terhadap Bakteri Staphylococcus aureus dan Escherichia coli

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Michele Sumampouw
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Potato Dextrose Agar ◽  
Rhizophora Stylosa ◽  
E Coli

Abstract: The aim of this study is to investigate the antibacterial effect of endophytic fungi originated from the root of mangrove Rhizophora stylosa growing on Mangrove Plantation Area around DAS Simpang 5 Jl. Piere Tendean Manado. The method of this research was using combination of PDA and NA media. Two species of endophytic fungi were extracted from the root of R. stylosa, black mycelium fungi as isolate A and brown mycelium fungi as isolate B. The activities of both isolates were tested against pathogenic bacteria Staphylococcus aureus and Escherichia coli. The result showed the same inhibition zone of isolate A and B to S. aureus, while, isolate A showed bigger the inhibition zone in comparison to isolate B against E. coli. As a conclusion, the endophytic fungi taken from the root of R. stylosa have antibacterial activity toward S. aureus and E. coli. Keywords: endophytic fungi, Rhizophora stylosa, antibacterial, Staphylococcus aureus, Escherichia coli.   Abstrak: Penelitian ini bertujuan untuk melihat adanya efek antibakteri dari jamur endofit yang diambil dari akar bakau Rhizophora stylosa yang ditanam di sekitar Perairan Daerah Aliran Sungai Simpang Lima Jl. Piere Tendean Manado. Metode penelitian dilakukan dengan menggunakan kombinasi media Potato Dextrose Agar (PDA) dan Nutrient Agar (NA). Dari akar bakau R. stylosa diperoleh dua  jenis jamur endofit yaitu isolat A dengan karateristik miselium jamur berwarna hitam dan isolat B yang dengan karakteristik miselium berwarna coklat. Kedua isolat ini selanjutnya diujikan aktivitasnya terhadap bakteri Staphylococcus aureus dan Escherichia coli. Hasil penelitian menunjukkan isolat A dan B memberikan  zona hambat yang sama terhadap bakteri Staphylococcus aureus dan pada bakteri Escherichia coli isolat A menunjukkan diameter zona hambat yang lebih besar dibandingkan isolat B. Kesimpulan, Jamur endofit yang diisolasi dari akar bakau R. Stylosa memiliki efek antibakteri terhadap pertumbuhan bakteri S. aureus dan E. coli. Kata kunci: jamur endofit, Rhizophopra stylosa, antibakteri, Staphylococcus aureus, Escherichia coli.

scholarly journals UJI AKTIVITAS ANTIBAKTERI JAMUR ENDOFIT AKAR BAKAU Avicennia marina TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coli

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Firdy Liwang
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Endophytic Fungi ◽  
Inhibition Zone ◽  
Avicennia Marina ◽  
Positive Control ◽  
Potato Dextrose Agar ◽  
Fungal Mycelium ◽  
Tidal Zone ◽  
E Coli

Abstract: In this his study we used endophytic fungi isolated from the roots of mangrove Avicennia marina growing on tidal zone around Tasik Ria Minahasa, North Sulawesi. The fungi were isolated and then tested the antibacterial effect against Staphylococcus aureus and Escherichia coli. Potato Dextrose agar was used in order to isolate the target fungi. The fungi began to grow on the second day after inoculation. Differentiation and purification processes to isolate the fungus obtained by observing fungi mycelia. While, the antibacterial activity test was following agar diffusion assay by planting 10 mm diameter of fungal mycelium together with particular bacteria onto the combination media. Ciprofloxacin was used as a positive control. The results shows a visible bacterial inhibition zone around the growing fungi with a diameter between 18 mm to 20.4 mm. Based on the results, it can be concluded that endophytic fungi isolated from the roots of mangrove Avicennia marina is able to inhibit the growth of S.aureus and E. coli. Keyword: Avicennia marina, Antibacterial, endophytic fungi   Abstrak: Penelitian ini menggunakan jamur endofit yang diisolasi dari akar tumbuhan bakau Avicennia marina yang tumbuh di perairan sekitar Tasik Ria Minahasa, Sulawesi Utara. Jamur endofit yang diisolasi kemudian diuji efek antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli. Isolasi jamur endofit menggunakan media PDA (Potato Dextrose Agar). Jamur mulai tumbuh pada hari kedua setelah inokulasi. Proses pemurnian jamur diperoleh dengan mengisolasi jamur dengan membedakan miselia. Pengujian efek antibakteri mengikuti prosedur agar difusi dengan menanam miselium jamur sebesar 10 mm pada media kombinasi yang telah diolesi bakteri uji. Sebagai kontrol positif digunakan Ciprofloxacin. Hasil penelitian terlihat diameter zona hambat pertumbuhan bakteri yang terbentuk sebesar 18 mm hingga 20,4 mm. Berdasarkan hasil penelitian maka dapat disimpulkan jamur endofit yang diisolasi dari akar tumbuhan bakau Avicennia marina mampu menghambat pertumbuhan bakteri S. aureus dan bakteri E. coli. Kata kunci: Avicennia marina, antibakteri, jamur endofit

scholarly journals Isolation and Antibacterial Activity Test of The Extract Ethyl Acetate of Endophytic Fungi from Kencur (Kaempferia Galanga L.)

2020 ◽  
Vol 3 (2) ◽  
pp. 85-92
Author(s):  
M. Rifqi Efendi ◽  
Mesa Sukmadani Rusdi ◽  
Fitria Anisa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Potato Dextrose Agar ◽  
Vibrio Cholera ◽  
Kaempferia Galanga ◽  
Activity Test ◽  
Fusarium Sp

Penelitian ini bertujuan untuk mengisolasi jamur endofit yang terdapat pada daun dan rimpang kencur (Kaempferia galanga L.) serta menguji aktivitas antibakterinya. Isolasi jamur endofit dilakukan dengan kultivasi dan subkultivasi jamur yang tumbuh dari daun dan rimpang kencur (Kaempferia galanga L.) yang sebelumnya telah disterilkan pada media potato dextrose agar (PDA). Identifikasi jamur endofit dilakukan secara makroskopis dan mikroskopis terhadap morfologinya. Pengujian aktivitas antibakteri dilakukan menggunakan metode difusi agar. Dari daun dan rimpang kencur diperoleh lima isolat jamur endofit  yang diidentifikasi sebagai Torulla sp. (KG001), Mucor sp. (KG002), Fusarium sp. (KG003), Geotricum sp. (KG004), dan Drechslera sp. (KG005). Tiga ekstrak etil asetat jamur endofit Torulla sp. (KG001), Fusarium sp. (KG003), dan Drechcera sp. (KG005) memiliki aktivitas sebagai antibakteri terhadap bakteri Gram positif  (Staphylococcus aureus and Vibrio cholera) dan Gram negatif (Bacillus subtilis and Eschericia coli) pada konsentrasi 3.75 %. Jamur endofit yang memiliki aktivitas antibakteri kuat (diameter hambat 10-20 mm) adalah Torulla sp. (KG001) dan Drechslera sp. (KG005). Sedangkan, jamur endofit yang memiliki aktivitas antibakteri sedang (diameter hambat 5-10 mm) adalah ekstrak etil asetat jamur Fusarium sp. (KG003). Aktivitas antibakteri terbesar ditunjukkan oleh ekstrak etil asetat isolat jamur Drechslera Sp. dengan diameter hambat 16 mm terhadap bakteri uji Vibrio cholera.

scholarly journals UJI EFEK ANTIBAKTERI JAMUR ENDOFIT AKAR TUMBUHAN BAKAU (SONNERATIA ALBA) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIAE COLI

2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Titi L. Faraknimella ◽  
Robert Bara ◽  
Pemsi M. Wowor ◽  
Jimmy Posangi
Keyword(s):  
Staphylococcus Aureus ◽  
Endophytic Fungi ◽  
Antibacterial Effect ◽  
Potato Dextrose Agar ◽  
Nutrient Agar ◽  
Combination Method ◽  
Mangrove Species ◽  
Antimicrobial Effects ◽  
Black And White ◽  
Sonneratia Alba

Abstract: Mangrove has been known long to have many benefits. One of the mangrove species that believed to have antimicrobial effects is Sonneratia alba. The objective of the study was to evaluate any antibacterial effect of the endophytic fungi isolated from the roots of Sonneratia alba toward the growth of Staphylococcus aureus and Eschericiae coli, using the combination method of PDA (Potato Dextrose Agar) and NA (Nutrient Agar). From the result we found two types of endophytes. Black and white colored fungus obtained from Sonneratia alba roots. Both fungi have the effect to inhibit the growth of Staphylococcus aureus and Eschericiae coli bacterial tested.Keywords: endophyte, Sonneratia alba, antibacterial, staphylococcus aureus, escherichiae coli.Abstrak: Tumbuhan bakau sejak lama telah diketahui memiliki banyak khasiat. Salah satu jenis tumbuhan bakau yang diyakini memiliki efek antimikroba adalah Sonneratia alba. Penelitian ini dilakukan bertujuan untuk melihat adanya efek antibakteri dari jamur endofit yang diambil dari akar tumbuhan bakau Sonneratia alba terhadap pertumbuhan bakteri Staphylococcus aureus dan Eschericiae coli. Metode penilitian yang digunakan dengan cara kombinasi PDA (Potato Dextrose Agar) dan NA (Nutrient Agar). Hasil penelitian di dapatkan dua jenis miselia jamur endofit. Jamur endofit berwarna hitam dan berwarna putih diperoleh dari akar tumbuhan bakau Sonneratia alba. Kedua jenis jamur ini memiliki efek daya hambat pada bakteri yang diujikan yaitu Staphylococcus aureus dan Escherichiae Coli.Kata kunci: jamur endofit, Sonneratia alba, antibakteri, staphylococcus aureus, escherichiae coli.

scholarly journals An antibiotic agent pyrrolo[1,2-a]pyrazine-1,4-dione,hexahydro isolated from a marine bacteria Bacillus tequilensis MSI45 effectively controls multi-drug resistant Staphylococcus aureus

RSC Advances ◽  
2018 ◽  
Vol 8 (32) ◽  
pp. 17837-17846 ◽  
Author(s):  
George Seghal Kiran ◽  
Sethu Priyadharsini ◽  
Arya Sajayan ◽  
Amrudha Ravindran ◽  
Joseph Selvin
Keyword(s):  
Staphylococcus Aureus ◽  
Marine Bacteria ◽  
Drug Resistant ◽  
Antibiotic Agent ◽  
Bacillus Tequilensis

A new antibiotic agent from sponge associated marine bacteria.

Cytochemical Studies of Cell Wall Biosynthesis in Staphylococcus Aureus

1972 ◽  
Vol 30 ◽  
pp. 328-329
Author(s):  
Masaatsu Koike ◽  
Koichi Nakashima ◽  
Kyoko Iida
Keyword(s):  
Staphylococcus Aureus ◽  
Periodate Oxidation ◽  
Early Time ◽  
The Other ◽  
Penicillin G ◽  
Cell Wall Biosynthesis ◽  
Septal Wall ◽  
Peptidoglycan Biosynthesis ◽  
Gram Positive Bacteria ◽  
Cross Linkage

Penicillin exerts the activity to inhibit the peptide cross linkage between each polysaccharide backbone at the final stage of wall-peptidoglycan biosynthesis of bacteria. Morphologically, alterations of the septal wall and mesosome in gram-positive bacteria, which were occurred in early time after treatment with penicillin, have been observed. In this experiment, these alterations were cytochemically investigated by means of silver-methenamine staining after periodate oxidation, which is applied for detection of localization of wall mucopolysaccharide.Staphylococcus aureus strain 209P treated with 100 u/ml of penicillin G was divided into two aliquotes. One was fixed by Kellenberger-Ryter's OSO4 fixative at 30, 60 and 120 min after addition of the antibiotic, dehydrated through alcohol series, and embedded in Epon 812 (Specimen A). The other was fixed by 21 glutaraldehyde, dehydrated through glycolmethacrylate series and embedded in glycolmethacrylate mixture, according to Bernhard's method (Specimen B).

Gold labeling of teichoic acid on adjacent surfaces of staphylococcus aureus

1990 ◽  
Vol 48 (3) ◽  
pp. 578-579
Author(s):  
Margaret Hukee
Keyword(s):  
Staphylococcus Aureus ◽  
Teichoic Acid ◽  
Protein Labeling ◽  
Minimal Amount ◽  
Section Thickness ◽  
Double Labeling ◽  
Parallel Surfaces ◽  
Supporting Membrane

Gold labeling of two antigens (double labeling) is often done on two section surfaces separated by section thickness. Whether labeling is done on both sides of the same section or on two parallel surfaces separated by section thickness (PSSST), comparable results are dependent on an equal number of epitopes being exposed at each surface. We propose a method to study protein labeling within the same field of proteins, by examining two directly adjacent surfaces that were split during sectioning. The number of labeling sites on adjacent surfaces (AS) were compared to sites on PSSST surfaces in individual bacteria.Since each bacteria needed to be recognizable in all three section surfaces, one-hole grids were used for labeling. One-hole grids require a supporting membrane and excessive handling during labeling often ruptures the membrane. To minimize handling, a labeling chamber was designed that is inexpensive, disposable, minimizes contamination, and uses a minimal amount of solution.

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