scholarly journals DIFFERENCES VALUE OF PT AND APTT IN EXAMINATION OF ELECTROMECHANICAL AND PHOTO-OPTICAL METHOD

2021 ◽  
Vol 5 (1) ◽  
pp. 17
Author(s):  
Beytri Ramadhani
Keyword(s):  
Optical Method ◽  
Clinical Pathology ◽  
Plasma Viscosity ◽  
Pathology Laboratory ◽  
Optical Techniques ◽  
Clinical Laboratories ◽  
The Difference ◽  
Detection Principle

Background: Examination of PT and APTT in hospitals and clinical laboratories by utilizing the use of different instruments and methods. Examination of PT and APTT can be implemented through electromechanical or photo-optical techniques to detect changes in plasma turbidity. This method principle isprincipled that addition can affect the increase in plasma viscosity. Purpose: To analyze the differences in the values of PT and APTT between the electromechanical method and the photo-optical method. Method: Analytical observation, 32 plasma citrate without interference and hemolysis were examined at the Clinical Pathology Laboratory of RSU Haji Surabaya and the Ultra Medica Main Clinics Laboratory in Surabaya. The study used SPSS 24.0 program to determine whether there were  PT and APTT values with an electromechanical and photo-optical method. Result: The result of PT and APTT with the electromechanical method was significantly higher than PT and APTT with the photo-optical method on samples without interference. In hemolytic samples, the result of PT with the electromechanical method was significantly higher than the PT result with the photo-optical method. Meanwhile, the result of APTT with the electromechanical method was significantly lower than the APTT result with the photo-optical method in hemolytic samples. Conclusion: There were significant differences in PT and APTT results between electromechanical and photo-optical in samples without interference and hemolytic. It is due to the difference in the detection principle between the two methods.

Clinical Laboratories Accreditation Program of the Brazilian Society of Clinical Pathology/Laboratory Medicine (PALC/SBPC-ML)

2015 ◽  
Vol 30 (3) ◽  
pp. 294-295
Author(s):  
Wilson Shcolnik ◽  
Carla Chaves ◽  
Carlos Eduardo dos Santos Ferreira ◽  
Cesar Sanches ◽  
Erlo Roth ◽  
...  
Keyword(s):  
Clinical Pathology ◽  
Laboratory Medicine ◽  
Pathology Laboratory ◽  
Brazilian Society ◽  
Clinical Laboratories ◽  
Accreditation Program

scholarly journals DIFFERENCES IN LED VALUES IN DENGUE FEVER PATIENTS WITH HEMOCONCENTRATION AND NOT HEMOCONCENTRATION

2019 ◽  
Vol 3 (2) ◽  
pp. 63
Author(s):  
Rismita Pritanti ◽  
Paulus Budiono Notopuro
Keyword(s):  
Blood Sample ◽  
Erythrocyte Sedimentation Rate ◽  
Dengue Fever ◽  
Sedimentation Rate ◽  
Plasma Proteins ◽  
Clinical Pathology ◽  
T Test ◽  
Pathology Laboratory ◽  
Erythrocyte Sedimentation ◽  
The Difference

Background: Patients with dengue fever with hemoconcentration will experience plasma tearing (loss of plasma protein) which will affect the value of the Erythrocyte Sedimentation Rate (LED). Plasma proteins that play a role in influencing the value of the Sedimentation Rate (LED) are fibrinogen and globulins. An erythrocyte sedimentation (LED) examination measures the degree of erythrocyte deposition in a blood sample over a period of time. LEDs are sensitive but not specific tests. Purpose: To determine the difference in LED values of DHF patients with hemoconcentration and not hemoconcentration. Methods: Observational analytic, 30 samples with criteria for dengue fever patients with hemoconcentration and not hemoconcentration were examined at the Clinical Pathology Laboratory of Haji Hospital Surabaya in March - April 2019. The study used an independent t test with SPSS 16.0 program to determine whether there were differences in LED values in dengue fever patients with hemoconcentration and not hemoconsetration. Results: The results of the Independent t test showed that there were significant differences between the results of the erythrocyte sedimentation rate in patients with dengue fever with hemoconcentration and not hemoconsetration with Sig. (2-failed) of 0.00 (p <0.005). Conclusion: There are significant differences in sediment rate results with the Westergreen blood method in patients with dengue fever who experience hemoconcentration and who do not experience hemoconcentration.

scholarly journals Indispensability of Clinical Bioinformatics for Effective Implementation of Genomic Medicine in Pathology Laboratories

ACI Open ◽  
2020 ◽  
Vol 04 (02) ◽  
pp. e167-e172
Author(s):  
Srikar Chamala ◽  
Siddardha Majety ◽  
Shesh Nath Mishra ◽  
Kimberly J. Newsom ◽  
Shaileshbhai Revabhai Gothi ◽  
...  
Keyword(s):  
Patient Care ◽  
Clinical Laboratory ◽  
Clinical Care ◽  
Genomic Medicine ◽  
Clinical Pathology ◽  
Genetic Mutation ◽  
Genomic Research ◽  
Pathology Laboratory ◽  
Clinical Bioinformatics ◽  
Effective Implementation

AbstractPatient care is rapidly evolving toward the inclusion of precision genomic medicine when genomic tests are used by clinicians to determine disease predisposition, prognosis, diagnosis, and improve therapeutic decision-making. However, unlike other clinical pathology laboratory tests, the development, deployment, and delivery of genomic tests and results are an intricate process. Genomic technologies are diverse, fast changing, and generate massive data. Implementation of these technologies in a Clinical Laboratory Improvement Amendments-certified and College of American Pathologists-accredited pathology laboratory often require custom clinical grade computational data analysis and management workflows. Additionally, accurate classification and reporting of clinically actionable genetic mutation requires well-curated disease/application-specific knowledgebases and expertise. Moreover, lack of “out of the box” technical features in electronic health record systems necessitates custom solutions for communicating genetic information to clinicians and patients. Genomic data generated as part of clinical care easily adds great value for translational research. In this article, we discuss current and future innovative clinical bioinformatics solutions and workflows developed at our institution for effective implementation of precision genomic medicine across molecular pathology, patient care, and translational genomic research.

Use of cost-effective technologies for a routine clinical pathology laboratory

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Mercedes Vazquez ◽  
L. Anfossi ◽  
Hader Ben-Yoav ◽  
Lorena Diéguez ◽  
Thomas Karopka ◽  
...  
Keyword(s):  
Developing Countries ◽  
Clinical Pathology ◽  
Cost Effective ◽  
Limiting Factor ◽  
Economic Costs ◽  
Pathology Laboratory

Classically, the need for highly sophisticated instruments with important economic costs has been a major limiting factor for clinical pathology laboratories, especially in developing countries. With the aim of making...

scholarly journals PENETUAN FORMULA UNTUK MENETAPKAN ESTIMASI KOLESTEROL-LDL

2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Gilang Nugraha ◽  
Soebagijoh Poegoeh Edijanto
Keyword(s):  
Correlation Coefficient ◽  
Ldl Cholesterol ◽  
Clinical Pathology ◽  
Statistical Test ◽  
Validation Methods ◽  
Clinical Laboratories ◽  
Friedewald Formula

Abstract: Measurement LDL-Cholesterol (LDL-C) can be performed by estimating LDL-C (cLDL-C) inthe blood. In addition to the first proposed Friedewald formula, several new formulas that promise betterexamination in determining LDL-C levels, proposed by Anandaraja, Puavilai, Chen, Vujovic, deCordova and Dansethakul. The subjects of the study were patients who performed routine lipid profileexamination in Institution of Clinical Pathology of Gedung Pusat Diagnostik Terpadu RSUD Dr.Soetomo Surabaya who performs 10 to 12 hours of fasting with TG less than 400 mg / dL. The sampleconsisted of 41 men and 48 women with an average age of 52 years. The statistical test on each cLDLC gave the Friedewald -1.32% bias value; Anandaraja -3,92%; Puavilai 4.26%, Chen -2.19%; Vujovic-23.65%; de Cordova -0.91% and Dansethakul 4.45%. The correlation coefficient on Friedewald0.9509; Anandaraja 0.9013; Puavilai 0.9576, Chen 0.9585; Vujovic 0.8745; de Cordova 0.9300 andDansethakul 0.9505. The proposed cLDL-C formula Chen et al promises in determining the LDL-Cestimate and the Vujovic formula gives a poor result in this study. Validation methods should be appliedto each of the cLDL formulas if they are to be applied to clinical laboratories.

scholarly journals ANALISIS BEBAN KERJA DI INSTALASI LABORATORIUM

2016 ◽  
Vol 20 (1) ◽  
pp. 64
Author(s):  
Amiroh Kurniati ◽  
Tahono Tahono
Keyword(s):  
Clinical Pathology ◽  
Work Load ◽  
Accurate Method ◽  
Development Planning ◽  
Smear Microscopy ◽  
Pathology Laboratory ◽  
Laboratory Installation ◽  
Laboratory Service ◽  
Laboratory Staff ◽  
Manpower Needs

Human resources (HR) planning is associated with an institution’s strategic plan, so the study of the required number of support personnel is in line with the direction of business development planning of the Laboratory Installation Unit. WISN (work load indicator staff need) method is a calculation based on the analysis of health manpower needs of the workload (work load analysis) and is considered as the most accurate method to calculate labour requirements in short-term. Based on the results of the analysis of manpower needs at the Laboratory Installation of Lung Health of the Community Hall Surakarta, it is known that there is still a lack of a number of five (5) persons of the health laboratory staff. The persons still lacking consist of: one (1) laboratory person related to clinical pathology laboratory, one (1) laboratory person for smear microscopy laboratory, and still three (3) microbiology laboratory persons. These persons are needed to be able to carry out the activities for a good laboratory service, and quality which is in accordance with the related applicable standards.

Selection of Hormonal Reference Values for Undescended Testicle Case in 8-Year-Old Boy with Abnormal Chromosome

2021 ◽  
Vol 28 (1) ◽  
pp. 91
Author(s):  
Vina Corry ◽  
Merci M. Pasaribu
Keyword(s):  
Reference Values ◽  
Clinical Pathology ◽  
Tanner Stage ◽  
Chromosome Analysis ◽  
Reference Intervals ◽  
Undescended Testicle ◽  
Pediatric Endocrinology ◽  
Pathology Laboratory ◽  
Testosterone Levels ◽  
And Gender

Establishing the diagnosis of undescended testicles requires appropriate hormonal laboratory reference values basedon age and gender. An 8-year-old boy with an undescended testicle, mental retardation, and stunting had a blood test thatwas carried out at the Clinical Pathology Laboratory, dr. Cipto Mangunkusumo (RSCM) Hospital on February 6, 2020, withtestosterone levels of 0.69 nmol/L (N male: 4.94-32.01 nmol/L) indicating decreased testosterone levels. The patient wasconsulted from urological surgery to pediatric endocrinology to determine the presence or rudiment of the patient'stesticles. Using the reference range of testosterone values assists clinicians in determining the diagnosis, monitoringtherapy, and prognosis of a disease. There are some testosterone reference values, which are currently available, includingCanadian Laboratory Initiative on Pediatric Reference Intervals Database (CALIPER) and the Tanner stage reference value.Later is more applicable because it is based on chronological age and secondary sexual development in assessing pubertydevelopment. A case of an 8-year-old boy with a clinical diagnosis of an undescended testicle, the laboratory test resultsshowed normal-low testosterone levels using the CALIPER and Tanner stage ranges according to the patient's age. Noincrease of testosterone levels after the second HCG stimulation test might be due to differences in the HCG administrationprotocol; therefore, the diagnosis of anorchia had not been established, and chromosome abnormalities of 46 XY, +6 Mar,17 dmin on chromosome analysis suggested the suspected syndrome. These findings were consistent with the suspicion ofprimary hypogonadism in children with suspected syndrome caused by bilateral cryptorchidism with a suspectedseminiferous tubular defect.

scholarly journals EVALUATION OF CASPASE 3 AS AN INDICATOR FOR BREAST CANCER CHEMOTHERAPY RESPONSE

2018 ◽  
Vol 11 (8) ◽  
pp. 259
Author(s):  
Kamal Basri Siregar ◽  
Tjakra Wibawa Manuaba ◽  
Muhammad Najib Dahlan Lubis ◽  
Rosita Juwita Sembiring
Keyword(s):  
Breast Cancer ◽  
Clinical Response ◽  
Caspase 3 ◽  
Chemotherapy Response ◽  
Pathology Laboratory ◽  
Breast Cancer Chemotherapy ◽  
Breast Tumor Tissue ◽  
Significant Difference ◽  
The Difference ◽  
Tnbc Subtype

Objectives: This study aimed to assess the difference of postchemotherapy caspase 3 level between triple negative breast cancer (TNBC) subjects with and without clinical response.Methods: A total of 48 subjects with intraductal and 12 subjects with intralobular TNBC who were undergoing surgery at Adam Malik General Hospital were analyzed the response of neoadjuvant chemotherapy. Postsurgical breast tumor tissue was treated in pathology laboratory for caspase 3 analysis. The data were processed in SPSS 22 with significance limitation of 0.05.Results: Median levels of caspase 3 postchemotherapy were higher both in intraductal and intralobular TNBC subtype (6 vs. 4.5 and 5 vs. 3, respectively) responsive group, while no changes detected in the group without clinical response. In statistical analysis, there was a significant difference of caspase 3 level postchemotherapy only in group with clinical response (p=0.005 in intraductal carcinoma and p=0.0031 in intralobular carcinoma).Conclusion: Postchemotherapy caspase 3 level increased significantly in TNBC, either intraductal or intralobular subtype, subjects with clinical response, but not in subjects without clinical response. 

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