scholarly journals PYTHOCHEMICAL SCREENING OF Musa acuminata STEM WATER EXTRACT

2020 ◽  
Vol 5 (1) ◽  
pp. 77
Author(s):  
Amy Nindia Carabelly ◽  
Didit Aspriyanto
Keyword(s):  
Growth Factor ◽  
Culture Media ◽  
Condensed Tannin ◽  
Water Extract ◽  
Hydrolysable Tannin ◽  
Cell Culture Media ◽  
Water Solvent ◽  
Stem Water ◽  
Banana Stem

Background: Regenerative therapy has been progressing into the utilization of mesenchymal stem cell (MSC). Nevertheless, the limited number of MSC has put growth factor as an essential supplement for cell culture media yet relatively unaffordable because highly priced. Alternative compound which cost reasonably is required. Exogenous phytochemical material in herbal plant extract may increase the number of MSC, one of which is mauli banana stem. Purpose: To analyze secondary metabolites identified in mauli banana stem water extract. Method: Mauli banana stem was macerated using water solvent to be analyzed qualitatively for alkaloid, tannin, flavonoid, saponin, terpenoid, diterpen and steroid. Screening was followed by quantitative analysis to determine the total of alkaloid, flavonoid, condensed tannin and hydrolysable tannin. Result: Secondary metabolite compounds of mauli banana stem water extract were alkaloid (4.15%), hydrolysable tannin (1.055%), condensed tannin (0.42%) and flavonoid (0.31%). Conclusion: Mauli banana stem water extract has potential as alternative growth factor to increase the number of MSC in vitro.

scholarly journals THE TOXICITY OF MAULI BANANA (MUSA ACUMINATA) STEM WATER EXTRACT ON BONE MARROW MESENCHYMAL STEM CELL IN VITRO

2019 ◽  
pp. 184-186
Author(s):  
AMY NINDIA ◽  
DIDIT ASPRIYANTO ◽  
MAHARANI LAILLYZA APRIASARI ◽  
SELVIANA RIZKY
Keyword(s):  
Growth Factor ◽  
Water Extract ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Cell Culture Process ◽  
Control Group Design ◽  
Water Solvent ◽  
Stem Water ◽  
Banana Stem

Objective: Since mesenchymal stem cells (MSC) can differentiate into bone, cementum, and periodontal ligament, they can be used to treat aggressiveperiodontitis. The limited number of MSCs requires replenishment of growth factor in the cell culture process. Since growth factor is quite expensive,an alternative material is needed. Mauli banana stem has antioxidant and immunomodulatory properties. Methanol extract of Mauli banana stem isknown to be toxic toward MSCs; therefore, another solvent with a non-toxic effect is needed, such as a water solvent. We analyzed the toxicity of Maulibanana stem water extract on MSC in vitro.Methods: In this laboratory experimental (true experimental) study with a Post-test Only Control Group Design, MSC cultures were treated withMauli banana stem water extract at 10, 20, 40, 60, 80, and 100 mg/mL dosages. One group without any treatment served as a control group and onewas a media control group. Each group was incubated for 24 h and then was given 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidereagent and analyzed by an enzyme-linked immunosorbent assay (ELISA) reader.Results: One-way analysis of variance showed a significant difference.Conclusion: Mauli banana stem water extracts at 10, 20, 40, and 60 mg/mL were not toxic toward MSC in vitro, while dosages of 80 and 100 mg/mLdosage were toxic.

4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro and implantation rates

2020 ◽  
Author(s):  
Nuria Hernández ◽  
Marta López-Morató ◽  
Mario J Perianes ◽  
Soledad Sánchez-Mateos ◽  
Vanessa Casas-Rua ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Embryo Quality ◽  
Culture Media ◽  
Embryo Implantation ◽  
In Vivo Models ◽  
Endometrial Cells ◽  
Epidermal Growth ◽  
Binding Capability

Abstract Embryo implantation in the uterus is a critical step to achieve success following ART. Despite favorable uterine conditions, a great number of good quality embryos fail to implant, often for reasons that are unknown. Hence, improving the implantation potential of embryos is a subject of great interest. 4-Hydroxyestradiol (4-OH-E2), a metabolic product of estradiol produced by endometrial cells, plays a key role in endometrial–embryonic interactions that are necessary for implantation. Nonetheless, the effects of 4-OH-E2 on embryos obtained in vitro have not been yet described. This study was designed to determine whether culture media enriched in 4-OH-E2 could improve the quality and implantation rate of embryos obtained in vitro, using both in vitro and in vivo models. We also analyzed its effects on the epidermal growth factor (EGF)-binding capability of the embryos. Our results showed that the presence of 4-OH-E2 in the culture media of embryos during the morula to blastocyst transition increases embryo quality and attachment to endometrial cells in vitro. 4-OH-E2 can also improve viable pregnancy rates of mouse embryos produced in vitro, reaching success rates that are similar to those from embryos obtained directly from the uterus. 4-OH-E2 improved the embryos’ ability to bind EGF, which could be responsible for the increased embryo implantation potential observed. Therefore, our results strongly suggest that 4-OH-E2 is a strong candidate molecule to supplement human IVF culture media in order to improve embryo implantation. However, further research is required before these findings can be translated with efficacy and safety to fertility clinics.

scholarly journals Impact of serum in cell culture media on in vitro lactate dehydrogenase (LDH) release determination

2017 ◽  
Vol 3 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Bernhard Hiebl ◽  
Sinem Peters ◽  
Ole Gemeinhardt ◽  
Stefan M. Niehues ◽  
Friedrich Jung
Keyword(s):  
Cell Culture ◽  
Lactate Dehydrogenase ◽  
Culture Media ◽  
Cell Culture Media ◽  
Ldh Release

scholarly journals Inhibition of α-Amylases by Condensed and Hydrolysable Tannins: Focus on Kinetics and Hypoglycemic Actions

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Camila Gabriel Kato ◽  
Geferson de Almeida Gonçalves ◽  
Rosely Aparecida Peralta ◽  
Flavio Augusto Vicente Seixas ◽  
Anacharis Babeto de Sá-Nakanishi ◽  
...  
Keyword(s):  
Condensed Tannin ◽  
Free Enzyme ◽  
Inhibitory Effects ◽  
Acacia Mearnsii ◽  
Hydrolysable Tannin ◽  
Mixed Inhibition ◽  
Hydrolysable Tannins ◽  
Kinetics Of

The aim of the present study was to compare the in vitro inhibitory effects on the salivary and pancreatic α-amylases and the in vivo hypoglycemic actions of the hydrolysable tannin from Chinese natural gall and the condensed tannin from Acacia mearnsii. The human salivary α-amylase was more strongly inhibited by the hydrolysable than by the condensed tannin, with the concentrations for 50% inhibition (IC50) being 47.0 and 285.4 μM, respectively. The inhibitory capacities of both tannins on the pancreatic α-amylase were also different, with IC50 values being 141.1 μM for the hydrolysable tannin and 248.1 μM for the condensed tannin. The kinetics of the inhibition presented complex patterns in that for both inhibitors more than one molecule can bind simultaneously to either the free enzyme of the substrate-complexed enzyme (parabolic mixed inhibition). Both tannins were able to inhibit the intestinal starch absorption. Inhibition by the hydrolysable tannin was concentration-dependent, with 53% inhibition at the dose of 58.8 μmol/kg and 88% inhibition at the dose of 294 μmol/kg. For the condensed tannin, inhibition was not substantially different for doses between 124.4 μmol/kg (49%) and 620 μmol/kg (57%). It can be concluded that both tannins, but especially the hydrolysable one, could be useful in controlling the postprandial glycemic levels in diabetes.

Growth hormone and parathyroid hormone stimulate IGFBP-3 in rat osteoblasts

1994 ◽  
Vol 267 (2) ◽  
pp. E226-E233 ◽  
Author(s):  
C. Schmid ◽  
I. Schlapfer ◽  
M. Peter ◽  
M. Boni-Schnetzler ◽  
J. Schwander ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Parathyroid Hormone ◽  
Growth Factor ◽  
Culture Media ◽  
Insulin Like Growth Factor ◽  
Igf I ◽  
Conditioned Cell Culture Medium ◽  
Rat Osteoblasts ◽  
Igfbp 3

Osteoblast-like cells prepared from calvaria of newborn rats produce insulin-like growth factor (IGF) I and several insulin-like growth factor binding proteins (IGFBPs) in vitro. Among the IGFBPs found in conditioned cell culture medium, IGFBP-3 is the most abundant. Intact IGFBP-3, as assessed by 125I-labeled IGF-II ligand blot analysis, is more abundant in culture media of cells exposed to growth hormone (GH) or to parathyroid hormone (PTH), both at 5 x 10(-9) mol/l, for 24 h. At the same time, concentrations of IGF-I are increased in media of cells exposed to PTH but not to GH, compared with hormone-free control cultures. IGFBP-3 mRNA is increased in osteoblasts exposed to PTH or to GH but not in response to 5 x 10(-9) mol/l IGF-I. PTH exerts a rapid (within 2 h) stimulatory effect on IGF-I and IGFBP-3 production, both at the message and peptide levels, whereas GH increases only IGFBP-3, both at the message and peptide levels (after 24 h). We conclude that IGF-I does not mediate increased IGFBP-3 production by rat osteoblasts in response to GH and PTH.

Resorption Mechanism of Hydroxyapatite and β-Tricalcium Phosphate Coating Layer

2008 ◽  
Vol 396-398 ◽  
pp. 81-84 ◽  
Author(s):  
Kang Sik Lee ◽  
Jae Suk Chang ◽  
Jung Hwa Kim ◽  
Chang Kuk You ◽  
Hoon Kwon ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Coating Layer ◽  
Culture Media ◽  
In Vitro Study ◽  
Cell Culture Media ◽  
Beta Tricalcium Phosphate ◽  
Ha Coating ◽  
Coating Method ◽  
Coating Layers

Beta-tricalcium phosphate(β-TCP) coating layer is known to be resorbed much faster than hydroxyapatite(HA), however, there has been no report to explain the exact reason of these results. Eighty titanium discs, coated with HA(n=40) or β-TCP(n=40) by dip and spin coating method, were divided into 2 subgroups respectively; Dissolution(D, n=20) and osteoclast culture(C, n=20). The coated discs in D group were immersed in the cell culture media for 5 days, whereas, in C group, osteoclasts were seeded on the specimens and cultured for 5 days. After simple dissolution test, β-TCP coating layer showed much more cracks and denudation as compared to HA. In osteoclast culture group, mean area fraction of resorption pits in HA-C group was 11.62%, which was significantly higher than that of 0.73% in β-TCP-C group(p=0.001). In conclusion, the resorption mechanisms of HA and β-TCP coating layers were different each other in vitro study. The coated β-TCP was degraded mainly by dissolution and separation from implant, on the other hand, the HA coating layer was resorbed by osteoclastic activity.

scholarly journals Insulin and Insulin-Like Growth Factor Stimulation of Vascular Endothelial Growth Factor Production by Luteinized Granulosa Cells: Comparison between Polycystic Ovarian Syndrome (PCOS) and Non-PCOS Women

2007 ◽  
Vol 92 (7) ◽  
pp. 2726-2733 ◽  
Author(s):  
Meghan B. Stanek ◽  
Sherri M. Borman ◽  
Theodore A. Molskness ◽  
Janine M. Larson ◽  
Richard L. Stouffer ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Granulosa Cells ◽  
Polycystic Ovarian Syndrome ◽  
Culture Media ◽  
Vascular Endothelial ◽  
Vitro Fertilization ◽  
Endothelial Growth Factor ◽  
Ovarian Syndrome

Abstract Context: Vascular endothelial growth factor A (VEGF-A) is a potent cytokine that promotes angiogenesis and vascular permeability. After controlled ovarian stimulation (COS) for in vitro fertilization (IVF), excessive VEGF-A production can occur, particularly in women with polycystic ovarian syndrome (PCOS); however, it is unclear whether the regulation of VEGF-A production is different between PCOS and non-PCOS women. Objective: The aim of this study was to determine whether there were differences in the dose- and time-dependent effects of insulin and IGFs on VEGF-A production by luteinized granulosa cells (LGCs) from women with and without PCOS. Design and Setting: A prospective comparative experimental study was conducted at an institutional practice. Patients: Patients included six PCOS and six non-PCOS women undergoing COS and IVF. Interventions: Interventions included COS for IVF. Main Outcome Measures: VEGF-A levels in culture media were collected daily for 3 d from LGCs after incubation with variable doses of insulin, IGF-I, and IGF-II in the presence and absence of LH. Results: In both study groups, exposure to LH alone did not alter VEGF-A levels. However, insulin or IGF increased VEGF-A levels within 1 d and appeared to synergize with LH at 3 d. VEGF-A production by non-PCOS LGCs was more sensitive to IGF exposure, whereas PCOS cells were more sensitive to insulin. Although an increase in DNA content (P < 0.05) was noted in cultures of PCOS cells, progesterone levels were lower compared with non-PCOS LGCs. Conclusion: Insulin and IGFs promote VEGF-A production in LGCs, but the response patterns are different when cells from PCOS and non-PCOS women are compared.

Trypanosoma musculi: In vitro cultivation of blood forms in cell culture media

1977 ◽  
Vol 7 (2) ◽  
pp. 109-111 ◽  
Author(s):  
P. Viens ◽  
M.C. Lajeunesse ◽  
R. Richards ◽  
G.A.T. Targett
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
In Vitro Cultivation ◽  
Cell Culture Media

scholarly journals EFFECT OF IN VITRO CULTIVATION ON THE PATHOGENICITY OF WEST NILE VIRUS

1946 ◽  
Vol 84 (2) ◽  
pp. 181-190 ◽  
Author(s):  
Hilary Koprowski ◽  
Edwin H. Lennette
Keyword(s):  
West Nile Virus ◽  
Culture Media ◽  
Lethal Effect ◽  
In Vitro Cultivation ◽  
Suspended Cell ◽  
West Nile ◽  
Cell Culture Media ◽  
Full Capacity ◽  
Old Mice

The West Nile virus was cultivated in suspended cell culture media employing several different tissue components, and it has been observed to survive in culture for at least 32 days. Continued propagation of the virus in vitro resulted in a change in its pathogenicity. The change lay in a marked reduction or a complete loss of the ability of the virus to produce fatal infections in mice and in hamsters on peripheral inoculation, although there was no obvious simultaneous alteration in the lethal effect of the virus by the cerebral route. In mice, the extent to which invasiveness was lost depended upon the passage level of the virus and the age of the test animals. The younger (and more susceptible) the mice, the greater the number of passages which was required to diminish the virulence of the virus by peripheral routes; after 68 passages, the virus still retained its full capacity to kill 3-day-old mice, while its ability to kill 8-day-old mice was reduced and its ability to kill mice 14 or more days of age was essentially abolished. How soon the loss of pathogenicity occurs in hamsters has not been determined. Prolonged cultivation rendered the virus avirulent for hamsters by the intraperitoneal route.

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