scholarly journals Application of Multi-Hurdles Technology in Development and Quality Evaluation of Shelf-Stable Barbecued Chicken Stored at Ambient Temperature

Author(s):  
P. Gunasekaran
2013 ◽  
Vol 93 (4) ◽  
pp. 535-542 ◽  
Author(s):  
K. Stanford ◽  
D. Gibb ◽  
T. A. McAllister

Stanford, K, Gibb, D. and McAllister, T. A. 2013. Evaluation of a shelf-stable direct-fed microbial for control of Escherichia coli O157 in commercial feedlot cattle. Can. J. Anim. Sci. 93: 535–542. A direct-fed microbial (DFM) registered for use in cattle in Canada containing Lactobacillus acidophilus strain BT-1386 and a Saccharomyces cerevisiae yeast autolysate was evaluated for control of E. coli O157. Weaned calves entered the feedlot in October and November and in January were sorted into Control (12 pens with a total of 2170 calves) and DFM treatment groups (10 pens with a total of 2040 calves). Although targeted dosage of L. acidophilus was 9 log10 colony forming units (CFU) head−1 d−1, analyses after storage at ambient temperature showed an average dose of 8.6 Log10 CFU head−1 d−1 and demonstrated stability of DFM over the range of temperatures encountered (−32.6 to 32.9°C) during storage. Calves entering the feedlot had low prevalence (0.8%) of E. coli O157 in feces, which increased to 11.2% in January. A 47°C range in ambient temperature for that month may have stressed cattle and led to increased shedding of E. coli O157 compared with seasonal norms. Comparing hide swabs collected at initiation of DFM feeding with those at shipping for slaughter, prevalence of E. coli O157 declined (P<0.05) in cattle fed DFM, although prevalence of E. coli O157 in hide swabs from Control and DFM-treated cattle did not differ at any time. As well, numbers of E. coli O157 and prevalence of the organism in fecal pats did not differ among treatments. Colonization of calves with E. coli O157 prior to DFM feeding likely reduced efficacy of DFM in the present study. Additional information regarding timing of feeding DFM relative to interactions among organisms within the gastrointestinal tract of cattle are required to ensure consistent efficacy of DFM for pre-harvest control of E. coli O157.


2016 ◽  
Vol 9 ◽  
pp. 90-93
Author(s):  
Sunil Rajbanshi ◽  
Bhaskar Mani Adhikari ◽  
Dilip Subba

A study was undertaken to develop shelf stable intermediate moisture type chicken meat pickle and evaluate its quality and storage stability. Pre-cooked and fried lean minced chicken meat was mixed with salt, spices and vinegar and packed air tight in glass jar. The product had appreciable sensory quality and low total microbial, yeast and mould counts. Salmonella, Staphylococci and E. coli were found absent in the product. Microbial count and peroxide value lay within the acceptable levels for sixty days.  


Author(s):  
Riya K. Zacharia ◽  
E. R. Aneena ◽  
Seeja Thomachan Panjikkaran ◽  
C. L. Sharon ◽  
P. S. Lakshmi

Aims: To standardise ready to eat millet based nutri flakes and to evaluate nutritional and shelf life qualities. Study Design: Completely randomized design Methodology: In the present study 5 treatments along with one control with 3 replications were standardized. Finger millet flour was used as the major ingredient and used in varying proportions starting from 50 to 80% along with 10 – 50 % of tapioca flour and 10 per cent other ingredients The best treatment were selected through sensory evaluation The selected treatment were evaluated for nutritional and shelf life qualities. The observations were tabulated and analysed statistically as completely randomized design (CRD). Results: Among various treatments, highest scores of organoleptic qualities was observed for  finger millet based nutri flakes added with tapioca flour (T3-60% FM + 30% TF +10% other ingredients) and it was selected as the best treatment with mean score of 7.92 for overall acceptability. The prepared product was nutritionally superior and highly acceptable throughout the storage. Conclusion: Nutri flakes based on 60% finger millet flour (FM) along with 30% TF was found to be the best combination for the preparation of nutri flakes and secured the highest mean score of 7.92 for overall acceptability. These nutri flakes were nutritionally superior and were shelf stable for 3 months.


Author(s):  
S.W. French ◽  
N.C. Benson ◽  
C. Davis-Scibienski

Previous SEM studies of liver cytoskeletal elements have encountered technical difficulties such as variable metal coating and heat damage which occurs during metal deposition. The majority of studies involving evaluation of the cell cytoskeleton have been limited to cells which could be isolated, maintained in culture as a monolayer and thus easily extracted. Detergent extraction of excised tissue by immersion has often been unsatisfactory beyond the depth of several cells. These disadvantages have been avoided in the present study. Whole C3H mouse livers were perfused in situ with 0.5% Triton X-100 in a modified Jahn's buffer including protease inhibitors. Perfusion was continued for 1 to 2 hours at ambient temperature. The liver was then perfused with a 2% buffered gluteraldehyde solution. Liver samples including spontaneous tumors were then maintained in buffered gluteraldehyde for 2 hours. Samples were processed for SEM and TEM using the modified thicarbohydrazide procedure of Malich and Wilson, cryofractured, and critical point dried (CPD). Some samples were mechanically fractured after CPD.


Author(s):  
S. Mahajan

The evolution of dislocation channels in irradiated metals during deformation can be envisaged to occur in three stages: (i) formation of embryonic cluster free regions, (ii) growth of these regions into microscopically observable channels and (iii) termination of their growth due to the accumulation of dislocation damage. The first two stages are particularly intriguing, and we have attempted to follow the early stages of channel formation in polycrystalline molybdenum, irradiated to 5×1019 n. cm−2 (E > 1 Mev) at the reactor ambient temperature (∼ 60°C), using transmission electron microscopy. The irradiated samples were strained, at room temperature, up to the macroscopic yield point.Figure 1 illustrates the early stages of channel formation. The observations suggest that the cluster free regions, such as A, B and C, form in isolated packets, which could subsequently link-up to evolve a channel.


Author(s):  
Robert C. Rau

Previous work has shown that post-irradiation annealing, at temperatures near 1100°C, produces resolvable dislocation loops in tungsten irradiated to fast (E > 1 MeV) neutron fluences of about 4 x 1019 n/cm2 or greater. To crystallographically characterize these loops, tilting experiments were carried out in the electron microscope on a polycrystalline specimen which had been irradiated to 1.5 × 1021 n/cm2 at reactor ambient temperature (∼ 70°C), and subseouently annealed for 315 hours at 1100°C. This treatment produced large loops averaging 1000 Å in diameter, as shown in the micrographs of Fig. 1. The orientation of this grain was near (001), and tilting was carried out about axes near [100], [10] and [110].


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