Therapeutic Effect of Idivallathi mezhugu on Lipid Peroxidation and Antioxidant System in 7, 12-dimethylbenz[a]anthracene Induced Mammary Tumour in Female Sprague-Dawley Rats

Objective: To determine the effect of Tridham (TD) and 1,2,3,4,6-penta-O-galloyl-β-d-glucose(PGG) on lipid peroxidation levels and mitochondrial antioxidants status in experimental mammary carcinoma.Methods: Elaecoarpus ganitrus (fruits), Terminalia chebula (seed coats), Prosopis cineraria (leaves), adult female albino rats of Sprague-Dawley strain weighing 170–190 g and 7,12-dimethylbenzeneanthracene (DMBA) were used for this study. Group I control rats, Group II rats mammary carcinoma induced with DMBA (25 mg in 1 ml olive oil) by gastric intubation. Group III, IV and V DMBA induced rats were treated with TD (400 mg/kg. b. wt/day), PGG (30 mg/kg. b. wt/day) and standard drug, Cyclophosphamide (30 mg/kg. b. wt/day), respectively for 48 d by gastric intubation. Group VI and VII rats served as TD and PGG treated controls, respectively for 48 d by gastric intubation. At the end of the experimental period, the rats were anaesthetized and sacrificed. Mammary glands were isolated and used for biochemical assays and histopathological evaluation.Results: In rats with cancer, the lipid peroxide levels (LPO) were significantly increased and mitochondrial antioxidant levels were decreased. Treatment with TD and PGG decreased LPO levels and increased mitochondrial antioxidant status in mammary carcinoma bearing rats. Histopathological analysis also confirmed the therapeutic effect of TD and PGG. No significant adverse effect was observed in sole drug treated group of rats.Conclusion: TD and PGG have definite therapeutic effect in experimental mammary carcinoma and inhibit growth of cancer cells by restoring mitochondrial antioxidant status and energy metabolism to normal states.

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Effect of Introducing the Jordanian Common Rue (Ruta chalepensis L.) on Liver Enzymes and Lipid Peroxidation in Adult Male Sprague Dawley Rats Toxified With Paracetamol

Journal of Agricultural Science ◽

10.5539/jas.v12n4p298 ◽

2020 ◽

Vol 12 (4) ◽

pp. 298

Author(s):

Dana N. Abdelrahim ◽

Hamed R. Takruri ◽

Khalid M. Al-Ismail

Keyword(s):

Lipid Peroxidation ◽

Hepatic Injury ◽

Liver Enzymes ◽

Liver Weight ◽

Normal Diet ◽

High Cholesterol Diet ◽

Sprague Dawley ◽

High Cholesterol ◽

Ruta Chalepensis ◽

Sprague Dawley Rats

This study aimed to determine the effect of Ruta chalepensis L. plant on liver enzymes, liver weight and lipid peroxidation using rats toxified with paracetamol. An animal experiment was conducted using five groups of Sprague Dawley rats, 9 rats each. The groups were fed: Normal diet, high cholesterol diet, with or without the plant or the liver toxicant paracetamol (PCM). The experiment lasted six weeks; at the end of the sixth week; a single dose of 3 g paracetamol/kg body weight was given for rats of two groups, then blood and liver samples were collected. The hepatoprotective effect of the plant was evaluated using aspartate aminotransferase (AST), alanine aminotransferase (ALT) and total bilirubin (TBL) levels as indicators. This study finds that the groups to which plant and PCM were given had significantly lower MDA levels in comparison with other groups that didn’t receive plant before PCM toxification. Tested liver enzymes levels were significantly (P < 0.05) lowered by the introduction of plant to the diet. Introducing PCM without ingestion of plant in the diet significantly (P < 0.05) increased the rats absolute liver weight. It is concluded that the use of Ruta chalepensis L. plant significantly lowered hepatic toxicity as indicated by the liver enzymes levels. Also, the plant lowered the MDA level and liver weight. The ingestion of the plant can be significantly protective against hepatic injury.

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Effects of99mTc sestamibi on antioxidant defense system and lipid peroxidation in the heart of Sprague Dawley rats

Toxicology and Industrial Health ◽

10.1177/0748233712452599 ◽

2012 ◽

Vol 30 (2) ◽

pp. 154-159 ◽

Cited By ~ 2

Author(s):

Gokhan Cesur ◽

Duygu Kumbul Doguc ◽

Mustafa Yildiz ◽

Serdal Ogut ◽

Mumin Polat ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Defense ◽

Antioxidant Defense System ◽

Defense System ◽

Sprague Dawley ◽

Sprague Dawley Rats

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Protective effect of L-carnitine against cisplatin-induced liver and kidney oxidant injury in rats

Open Medicine ◽

10.2478/s11536-009-0021-x ◽

2009 ◽

Vol 4 (2) ◽

pp. 184-191 ◽

Cited By ~ 8

Author(s):

Kerim Cayir ◽

Ali Karadeniz ◽

Abdulkadir Yildirim ◽

Yildiray Kalkan ◽

Akar Karakoc ◽

...

Keyword(s):

Lipid Peroxidation ◽

Therapeutic Potential ◽

Protective Effects ◽

Sprague Dawley ◽

Glutathione S Transferase ◽

Kidney Dysfunction ◽

Degree Of Protection ◽

Sprague Dawley Rats ◽

Liver And Kidney ◽

Endogenous Antioxidants

AbstractThe present study was designed to investigate the protective effects of L-carnitine (LC) on changes in the levels of lipid peroxidation and endogenous antioxidants induced by cisplatin (cis-diamminedichloroplatinum II, CDDP) in the liver and kidney tissues of rats. Twenty-four Sprague Dawley rats were equally divided into four groups of six rats each: control, cisplatin, L-carnitine, and L-carnitine plus cisplatin. The degree of protection produced by L-carnitine was evaluated by determining the level of malondialdehyde (MDA). The activity of glutathione (GSH), glutathione peroxidase (GSH-Px), glutathione S-transferase (GST), and superoxide dismutase (SOD) were estimated from liver and kidney homogenates, and the liver and kidney were histologically examined as well. L-carnitine elicited significant liver and kidney protective activity by decreasing the level of lipid peroxidation (MDA) and elevating the activity of GSH, GSHPx, GST, and SOD. Furthermore, these biochemical observations were supported by histological findings. In conclusion, the present study indicates a significant role for reactive oxygen species (ROS) and their relation to liver and kidney dysfunction, and points to the therapeutic potential of LC in CDDP-induced liver and kidney toxicity.

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Lipid peroxidation and scavenger enzymes during exercise: adaptive response to training

Journal of Applied Physiology ◽

10.1152/jappl.1988.64.4.1333 ◽

1988 ◽

Vol 64 (4) ◽

pp. 1333-1336 ◽

Cited By ~ 209

Author(s):

H. M. Alessio ◽

A. H. Goldfarb

Keyword(s):

Lipid Peroxidation ◽

Endurance Training ◽

Acute Exercise ◽

Oxidative Capacity ◽

Treadmill Running ◽

Moderate Intensity ◽

Sprague Dawley ◽

Moderate Intensity Exercise ◽

Sod Activity ◽

Sprague Dawley Rats

This study was designed to determine whether endurance training would influence the production of lipid peroxidation (LI-POX) by-products as indicated by malondialdehyde (MDA) at rest and after an acute exercise run. Additionally, the scavenger enzymes catalase (CAT) and superoxide dismutase (SOD) were examined to determine whether changes in LIPOX are associated with alterations in enzyme activity both at rest and after exercise. Male Sprague-Dawley rats (n = 32) were randomly assigned to either trained or sedentary groups and were killed either at rest or after 20 min of treadmill running. The training program increased oxidative capacity 64% in leg muscle. After exercise, the sedentary group demonstrated increased LIPOX levels in liver and white skeletal muscle, whereas the endurance-trained group did not show increases in LIPOX after exercise. CAT activity was higher in both red and white muscle after exercise in the trained animals. Total SOD activity was unaffected by either acute or chronic exercise. These data suggest that endurance training can result in a reduction in LIPOX levels as indicated by MDA during moderate-intensity exercise. It is possible that activation of the enzyme catalase and the increase in respiratory capacity were contributory factors responsible for regulating LIPOX after training during exercise.

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