Vinasse as a Sustainable Medium for the Production of Chlorella vulgaris UTEX 1803

This study investigates distillery wastewater, commonly known as vinasse, as a potential culture medium for the production of Chlorella vulgaris and its most relevant metabolites. The effect of vinasse concentration on the composition of the biomass (proteins, carbohydrates and lipids) was evaluated in treatments performed in 6-L tubular air-lift reactors. The reactors were operated at 25 °C for 18 days, in total darkness, under a continuous flow of air. Results showed a rapid growth of microalgae in the first ten days, when an average production of 0.87 g/L was reached. Then, the daily biomass productivity began to decrease, up to an average value of 11.8 g/L at the 16th day. For all treatments, there was a significant reduction in the concentration of most metabolites in the first eight days. This was likely due to the adaptation of the biomass to the new conditions, with a transition from autotrophic to heterotrophic metabolism. From the 10th day, the concentration of metabolites in the biomass began to increase, reaching a nearly constant value at the 16th day. The observed maximum concentrations (%w/w) were: 48.95% proteins, 2.88% xylose, 7.82% glucose, 4.54% arabinose, 8.28% fructose and 4.82% lipids. These values were only marginally affected by the type of treatment. Overall, the results obtained suggest that vinasse is a promising and sustainable medium for the growth of C. vulgaris and the production of valuable metabolites.

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Distillery Wastewater as a Sustainable Medium for Microalgae Production

10.20944/preprints201906.0184.v1 ◽

2019 ◽

Author(s):

Viviana Quintero-Dallos ◽

Janet Bibiana García-Martínez ◽

Jefferson Eduardo Contreras-Ropero ◽

Crisostomo Barajas-Ferrerira ◽

Andrés Fernando Barajas-Solano ◽

...

Keyword(s):

Chlorella Vulgaris ◽

Rapid Growth ◽

Continuous Flow ◽

Culture Medium ◽

Biomass Productivity ◽

Average Value ◽

Average Production ◽

Distillery Wastewater ◽

Heterotrophic Metabolism ◽

Air Lift

This study investigates distillery wastewater, commonly known as vinasse, as a potential culture medium for the production of Chlorella vulgaris and its most relevant metabolites. The effect of vinasse concentration on the composition of the biomass (proteins, carbohydrates and lipids) was evaluated in treatments performed in 6-L tubular air-lift reactors. The reactors were operated at 25 °C for 18 days, in total darkness, under a continuous flow of air. Results showed a rapid growth of microalgae in the first ten days, when an average production of 0.87 g/L was reached. Then, the daily biomass productivity began to decrease, up to an average value of 11.8 g/L at the 16th day. For all treatments, there was a significant reduction in the concentration of most metabolites in the first eight days. This was likely due to the adaptation of the biomass to the new conditions, with a the transition from autotrophic to heterotrophic metabolism. From the 10th day, the concentration of metabolites in the biomass began to increase, reaching a nearly constant value at the 16th day. The observed maximum concentrations (%w/w) were: 48.95% proteins, 2.88% xylose, 7.82% glucose, 4.54% arabinose, 8.28% fructose and 4.82% lipids. These values were only marginally affected by the type of treatment. Overall, the results obtained suggest that vinasse is a promising and sustainable medium for the growth of C. vulgaris and the production of valuable metabolites.

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Vinasse as a Sustainable Medium for the Production of Chlorella vulgaris UTEX 1803

Water ◽

10.3390/w11081526 ◽

2019 ◽

Vol 11 (8) ◽

pp. 1526 ◽

Cited By ~ 4

Author(s):

Viviana Quintero-Dallos ◽

Janet B. García-Martínez ◽

Jefferson E. Contreras-Ropero ◽

Andrés F. Barajas-Solano ◽

Crisostomo Barajas-Ferrerira ◽

...

Keyword(s):

Chlorella Vulgaris ◽

Rapid Growth ◽

Continuous Flow ◽

Culture Medium ◽

Biomass Productivity ◽

Average Value ◽

Average Production ◽

Distillery Wastewater ◽

Heterotrophic Metabolism ◽

Air Lift

This study investigates distillery wastewater, commonly known as vinasse, as a potential culture medium for the production of Chlorella vulgaris and its most relevant metabolites. The effect of vinasse concentration on the composition of the biomass (proteins, carbohydrates, and lipids) was evaluated in treatments performed in 6-L tubular air-lift reactors. The reactors were operated at 25 °C for 18 days, in total darkness, under a continuous flow of air. Results showed a rapid growth of microalgae in the first ten days, when an average production of 0.87 g/L was reached. Then, the daily biomass productivity began to decrease, up to an average value of 11.8 g/L at the 16th day. For all treatments, there was a significant reduction in the concentration of most metabolites in the first eight days. This was likely due to the adaptation of the biomass to the new conditions, with a transition from autotrophic to heterotrophic metabolism. From the 10th day, the concentration of metabolites in the biomass began to increase, reaching a nearly constant value at the 16th day. The observed maximum concentrations (%w/w) were: 48.95% proteins, 2.88% xylose, 7.82% glucose, 4.54% arabinose, 8.28% fructose, and 4.82% lipids. These values were only marginally affected by the type of treatment. Overall, the results obtained suggest that vinasse is a promising and sustainable medium for the growth of C. vulgaris and the production of valuable metabolites.

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Cultivation of Chlorella vulgaris in Membrane-Treated Industrial Distillery Wastewater: Growth and Wastewater Treatment

Frontiers in Environmental Science ◽

10.3389/fenvs.2021.770633 ◽

2021 ◽

Vol 9 ◽

Author(s):

Feng Li ◽

David Kwame Amenorfenyo ◽

Yulei Zhang ◽

Ning Zhang ◽

Changling Li ◽

...

Keyword(s):

Chlorella Vulgaris ◽

Environmentally Friendly ◽

Biomass Productivity ◽

Industrial Effluents ◽

Treatment Method ◽

Pollution Potential ◽

Microalgae Cultivation ◽

Microalgae Biomass ◽

Distillery Wastewater ◽

Removal Efficiencies

The alcohol industry discharges large quantities of wastewater, which is hazardous and has a considerable pollution potential. Cultivating microalgae in wastewater is an alternative way of overcoming the current high cost of microalgae cultivation and an environmentally friendly treatment method for industrial effluents. The study analyzed the growth and biochemical composition of Chlorella vulgaris cultivated in membrane-treated distillery wastewater (MTDW) and nutrients removal efficiency. The results showed biomass productivity of 0.04 g L−1 d−1 for MTDW with the contents of content of protein, carbohydrate, and lipid at 49.6 ± 1.4%, 26.1 ± 0.6%, and 10.4 ± 1.8%, respectively. The removal efficiencies of TN, TP, and COD were 80, 94, and 72.24% in MTDW, respectively. In addition, removal efficiencies of 100, 85.37, and 42.86% for Ca2+, Mg2+, and Mo2− were achieved, respectively. The study added to our growing knowledge on the cultivation of Chlorella with wastewater, suggesting that it was feasible to cultivate Chlorella with MTDW and represented an economical and environmentally friendly strategy for microalgae biomass production and reuse of wastewater resources.

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Cultivation of Chlorella vulgaris in anaerobically digested gelatin industry wastewater

Water Science & Technology Water Supply ◽

10.2166/ws.2020.263 ◽

2020 ◽

Author(s):

G. C. Blanco ◽

M. J. Stablein ◽

G. Tommaso

Keyword(s):

Chlorella Vulgaris ◽

Culture Medium ◽

High Efficiency ◽

Bubble Column ◽

Substrate Inhibition ◽

Basal Medium ◽

Biomass Productivity ◽

Negative Control ◽

Nitrogenous Compounds ◽

Industry Wastewater

Abstract This work aimed to study the effect of using anaerobically digested gelatin industry wastewater as a culture medium for the cultivation of Chlorella vulgaris microalgae in bubble column photobioreactors (PBRs). Batch experiments were carried out to determine the growth kinetics by inoculating microalgae in wastewater prepared with different dilutions and supplemented with Bold's Basal Medium (BBM). From the values of the saturation constants (KS = 50.25 mgN-NH4+·L−1) and substrate inhibition (KI = 28.12 mgN-NH4+·L−1) obtained in the adjustment to the Andrews kinetic model (R2 = 0.817), the PBRs achieved specific maximum growth rates (μmax) of 0.343 d−1, biomass productivity of 0.141 g·L−1·d−1, lipid content of 12.45%, lipid productivity of 17.63 mg·L−1·d−1 and instantaneous ammoniacal nitrogen consumption rates of 20.06 and 14.22 mg·L−1·d−1. The addition of wastewater to the culture medium provided an increase in biomass productivity of 57.45% in relation to the negative control. The results obtained demonstrate the high efficiency of C. vulgaris in the removal of nitrogenous compounds and the potential of using anaerobically digested gelatin industry wastewater in the production of microalgae biomass.

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Application of Reactor Engineering Concepts in Continuous Flow Chemistry: A Review

Reaction Chemistry & Engineering ◽

10.1039/d1re00004g ◽

2021 ◽

Author(s):

Nicole Candice Neyt ◽

Darren Lyall Riley

Keyword(s):

Rapid Growth ◽

Continuous Flow ◽

Flow Chemistry ◽

The Past ◽

Continuous Flow Chemistry

The adoption of flow technology for the manufacture of chemical entities, and in particular pharmaceuticals, has seen rapid growth over the past two decades with the technology now blurring the...

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A novel culture medium designed for the simultaneous enhancement of biomass and lipid production by Chlorella vulgaris UTEX 26

Bioresource Technology ◽

10.1016/j.biortech.2016.04.051 ◽

2016 ◽

Vol 212 ◽

pp. 207-216 ◽

Cited By ~ 11

Author(s):

Citlally Ramírez-López ◽

Isaac Chairez ◽

Luis Fernández-Linares

Keyword(s):

Chlorella Vulgaris ◽

Culture Medium ◽

Lipid Production

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Increase in lipid productivity and photosynthetic activities during distillery wastewater decolorization by Chlorella vulgaris cultures

Applied Microbiology and Biotechnology ◽

10.1007/s00253-021-11233-x ◽

2021 ◽

Author(s):

Seyed Mojtaba Soleymani Robati ◽

Mohsen Nosrati ◽

Faezeh Ghanati ◽

Abazar Hajnowrouzi ◽

Dominique Grizeau ◽

...

Keyword(s):

Chlorella Vulgaris ◽

Lipid Productivity ◽

Distillery Wastewater ◽

Photosynthetic Activities

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5462860 Conditioned culture medium for rapid growth and detection of microbes

Biotechnology Advances ◽

10.1016/s0734-9750(97)81911-8 ◽

1996 ◽

Vol 14 (4) ◽

pp. 555

Keyword(s):

Rapid Growth ◽

Culture Medium ◽

Conditioned Culture Medium

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Outdoor Cultivation of the Microalga Chlorella vulgaris in a New Photobioreactor Configuration: The Effect of Ultraviolet and Visible Radiation

Energies ◽

10.3390/en13081962 ◽

2020 ◽

Vol 13 (8) ◽

pp. 1962

Author(s):

Alcinda P. Lopes ◽

Francisca M. Santos ◽

Tânia F. C. V. Silva ◽

Vítor J. P. Vilar ◽

José C. M. Pires

Keyword(s):

Chlorella Vulgaris ◽

Phosphorus Uptake ◽

Biomass Productivity ◽

Nitrogen And Phosphorus ◽

Visible Radiation ◽

Microalgal Culture ◽

Wide Range ◽

Ultraviolet Irradiance ◽

Nitrogen And Phosphorus Uptake ◽

Specific Growth Rates

Microalgae can be a future source of biomass with a wide range of applications, including its use to solve current environmental issues. One of the main variables for microalgal cultivation is the light supply: (i) its intensity that often does not present a uniform spatial distribution inside the culture; (ii) photoperiod; and (iii) spectrum. Therefore, this study aims to evaluate the growth of the microalgae Chlorella vulgaris in a tubular photobioreactor with compound parabolic collectors (CPCs) under outdoor conditions. The effect of ultraviolet and visible radiation on biomass productivity and nutrients (nitrogen and phosphorus) uptake was assessed. The maximum biomass productivity was (5 ± 1) × 10−3 g·L−1·h−1, and the specific growth rates ranged from (1.1 ± 0.3) × 10−2 to (2.0 ± 0.6) × 10−2 h−1. Regarding nutrient uptake, initial removal rates of (0.9 ± 0.4) mg N·L−1·h−1 for nitrogen and (0.17 ± 0.04) mg P·L−1·h−1 for phosphorus were reached. These values increased with visible and ultraviolet irradiance until certain values (143 WVIS·m−2 and 9 WUV·m−2 for biomass productivity; 101 WVIS·m−2 and 6 WUV·m−2 for nutrient removal) and then decreased for higher ones due to the photoinhibition phenomenon. Therefore, the application of CPCs to photobioreactors (PBRs) may be beneficial for microalgal culture in countries with higher latitude (with lower solar irradiance levels).

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