scholarly journals Genome Wide Identification of Cotton C-Repeat Binding Factor (CBF) and Overexpression of Gthu17439 (GthCBF4) Gene Confer Cold Stress Tolerance in Arabidopsis Thaliana

2021 ◽  
Author(s):  
Jiangna Liu ◽  
Richard Magwanga ◽  
Yanchao Xu ◽  
Tingting Wei ◽  
Jie Zheng ◽  
...  
Keyword(s):  
Cold Stress ◽  
Stress Tolerance ◽  
Growth Yield ◽  
Forward Genetics ◽  
Genome Wide ◽  
Binding Factor ◽  
Growth Vigour ◽  
Yield Quality ◽  
Clade 1

Low temperature is a common biological abiotic stress in major cotton growing areas. Cold stress significantly affects the growth, yield and yield quality of cotton. Therefore, it is important to develop a more robust and cold stress tolerant cotton germplasms. Climate change and erratic climatic condition, plants have evolved various survival mechanisms, one of which induction of various stress responsive transcriptome factors, such as the C-repeat binding factor GthCBF4, which have been found to enhance cold tolerance in various plants. In this study detailed evaluation of the cotton C-repeat binding factor has been carried out. A total of29, 28, 25, 21, 30, 26 and 15 proteins encoded by the C-repeat binding factor were identified in G. herbaceum, G. arboreum, G. thurberi, G. raimondii, G. turneri, G. longicalyx and G. australe, respectively. Phylogeny evaluation revealed that the proteins were grouped into seven clades, with clade 1 and 6 being the largest. Moreover, majority of the proteins encoded by the genes were predicted to be located within. the nucleus, while some are distributed in other parts of the cell. Based on the transcriptome and RT-qPCR analysis, Gthu17439 (GthCBF4) was highly upregulated and was further validated through forward genetics. The Gthu17439 (GthCBF4) overexpressed plants showed a significantly tolerance to cold stress, with higher growth vigour compared to the wild types. The results showed that the Gthu17439 (GthCBF4) could be playing a significant role in enhancing cold stress tolerance in cotton and can be further exploited in developing a more cold stress tolerance cotton germplasm

scholarly journals Genome wide identification and functional characterization of the cotton C-repeat binding factor (CBF) under cold stress condition

2021 ◽  
Author(s):  
Jiangna Liu ◽  
Richard Magwanga ◽  
Yanchao Xu ◽  
Tingting Wei ◽  
Jie Zheng ◽  
...  
Keyword(s):  
Cold Stress ◽  
Stress Tolerance ◽  
Germination Rate ◽  
Functional Characterization ◽  
Growth Yield ◽  
Forward Genetics ◽  
Gossypium Raimondii ◽  
Gossypium Thurberi ◽  
Clade 1 ◽  
Gossypium Longicalyx

Abstract Background Low temperature is a common biological abiotic stress in major cotton growing areas. Cold stress significantly affects the growth, yield and yield quality of cotton. Therefore, it is important to develop a more robust and cold stress tolerant cotton germplasms. In response to climate change and erratic conditions, plants have evolved various survival mechanisms, one of which induction of various stress responsive transcription factors, such as the C-repeat binding factors (CBFs), which have been found to enhance cold tolerance in various plants. Results In this study detailed evaluation of the cotton CBF has been carried out. A total of 29, 28, 25, 21, 30, 26 and 15 proteins encoded by the CBF genes were identified in Gossypium herbaceum, Gossypium arboreum, Gossypium thurberi, Gossypium raimondii, Gossypium turneri, Gossypium longicalyx and Gossypium australe, respectively. Phylogenetic evaluation revealed that the proteins were grouped into seven clades, with clade 1 and 6 being the largest. Moreover, majority of the proteins encoded by the genes were predicted to be located within the nucleus, while some were distributed in other parts of the cell. Based on the transcriptome and RT-qPCR analysis, Gthu17439 (GthCBF4) was highly upregulated under cold stress, and was further validated through forward genetics. The Gthu17439 (GthCBF4) overexpressed plants showed a significantly tolerance to cold stress, with higher germination rate, higher root growth and high induction levels of stress responsive genes. The over-expressed plants exhibited low level of oxidative damage, due to significant reduction in the H2O2 production. Conclusion The results showed that the Gthu17439 (GthCBF4) could be playing a significant role in enhancing cold stress tolerance in cotton and can be further exploited in developing cotton germplasm with an improved cold-stress tolerance

scholarly journals Functional Characterization of Cotton C-Repeat Binding Factor Genes Reveal Their Potential Role in Cold Stress Tolerance

2021 ◽  
Vol 12 ◽  
Author(s):  
Jiangna Liu ◽  
Richard Odongo Magwanga ◽  
Yanchao Xu ◽  
Tingting Wei ◽  
Joy Nyangasi Kirungu ◽  
...  
Keyword(s):  
Cold Stress ◽  
Stress Tolerance ◽  
Cell Damage ◽  
Germination Rate ◽  
Functional Characterization ◽  
Growth Yield ◽  
Weather Conditions ◽  
Forward Genetics ◽  
Minimal Cell

Low temperature is a common biological abiotic stress in major cotton-growing areas. Cold stress significantly affects the growth, yield, and yield quality of cotton. Therefore, it is important to develop more robust and cold stress-resilient cotton germplasms. In response to climate change and erratic weather conditions, plants have evolved various survival mechanisms, one of which involves the induction of various stress responsive transcript factors, of which the C-repeat-binding factors (CBFs) have a positive effect in enhancing plants response to cold stress. In this study, genomewide identification and functional characterization of the cotton CBFs were carried out. A total of 29, 28, 25, 21, 30, 26, and 15 proteins encoded by the CBF genes were identified in seven Gossypium species. A phylogenetic evaluation revealed seven clades, with Clades 1 and 6 being the largest. Moreover, the majority of the proteins encoded by the genes were predicted to be located within the nucleus, while some were distributed in other parts of the cell. Based on the transcriptome and RT-qPCR analysis, Gthu17439 (GthCBF4) was highly upregulated and was further validated through forward genetics. The Gthu17439 (GthCBF4) overexpressed plants exhibited significantly higher tolerance to cold stress, as evidenced by the higher germination rate, increased root growth, and high-induction levels of stress-responsive genes. Furthermore, the overexpressed plants under cold stress had significantly reduced oxidative damage due to a reduction in hydrogen peroxide (H2O2) production. Moreover, the overexpressed plants under cold stress had minimal cell damage compared to the wild types, as evidenced by the Trypan and 3,3′-Diaminobenzidine (DAB) staining effect. The results showed that the Gthu17439 (GthCBF4) could be playing a significant role in enhancing cold stress tolerance in cotton and can be further exploited in developing cotton germplasm with improved cold-stress tolerance.

scholarly journals Selecting Aragonez Genotypes Able to Outplay Climate Change–Driven Abiotic Stress

2020 ◽  
Vol 11 ◽  
Author(s):  
Luísa Carvalho ◽  
Elsa Gonçalves ◽  
Sara Amâncio ◽  
Antero Martins
Keyword(s):  
Climate Change ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Cultural Practices ◽  
Abiotic Stress Tolerance ◽  
Quantitative Polymerase Chain Reaction ◽  
Experimental Population ◽  
Yield And Quality ◽  
Yield Quality

High temperatures and extreme drought are increasingly more frequent in Portugal, which represents a strong threat to viticulture in certain regions of the country. These multifactorial abiotic stresses are threatening viticultural areas worldwide, and the problem can hardly be overcome only by changing cultural practices. This scenario has raised a major challenge for plant scientists to find ways to adapt existing varieties to the new conditions without loss of their characteristic flavors, yield, and associated varietal character of wines. Aragonez (syn. Tempranillo) is one such variety, widely cultivated in Portugal and Spain, with specific characteristics associated with terroir. In this context, insight into intravarietal variability to enable its exploitation for selection becomes an important tool to mitigate the effect of multifactorial stresses driven by climate changes. The present work describes an innovative selection approach: selection for abiotic stress tolerance, measured by the leaf temperature of clones under environmental conditions of drought and extreme heat. This evaluation was complemented with values of yield and quality characteristics of the must (pH, acidity, °Brix, and anthocyanins). The application of this methodology was done in an experimental population of 255 clones of Tempranillo for 3 years. The genotypes were then ranked according to their level of tolerance to abiotic stress without loss of yield/quality. To understand the differences at the transcription level that could account for such variability, several of the most tolerant and most sensitive genotypes were analyzed for key genes using reverse transcriptase–quantitative polymerase chain reaction. The results enabled the selection of a group of genotypes with increased tolerance to stress, in relation to the average of the variety, which maintained the typical must quality of Aragonez. In parallel, several transcripts previously acknowledged as markers for abiotic stress tolerance were identified in several clones and are possible targets for plant breeding and genetic modification and/or to develop screening procedures to select genotypes better adapted to the abiotic stress driven by climate change.

Effect of irrigation based on IW/CPE ratio and sulphur levels on yield and quality of gram (Cicer arietinum L.)

2016 ◽  
Author(s):  
D. V. Srinivasulu ◽  
R. M. Solanki ◽  
M. Bhanuprakash ◽  
A. Vemaraju ◽  
P. J. Prajapati
Keyword(s):  
Water Use Efficiency ◽  
Water Use ◽  
Irrigation Treatment ◽  
Growth Yield ◽  
Yield Attributes ◽  
Net Return ◽  
Yield And Quality ◽  
Use Efficiency ◽  
Yield Quality

A field experiment was carried out during rabi 2010-11 to study the growth, yield and quality of gram as influenced by irrigation and sulphur levels. Irrigation and sulphur have shown significant influence on growth, yield, quality, moisture extraction pattern and water use efficiency. Among four irrigation schedules, irrigation scheduled at 0.9 IW/CPE ratio recorded higher values for all the growth parameters at various stages, yield attributes, grain and stover yield, quality parameters as well as net return and BCR while, 0.7 IW/CPE ratio remained on par. Amount of moisture extracted from surface layers was more irrespective of irrigation treatment. Depletion of soil moisture increased and water use efficiency decreased with increasing frequency of irrigation. Application of 40 kg S ha-1 recorded higher grain yield, protein content and protein yield, net return and BCR and remained on par with 20 kg S ha-1. However interaction between sulphur and irrigation levels, 20 kg S ha-1 and 0.7 IW/CPE has reported higher seed yield, net returns and BCR.

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