Genome wide identification and functional characterization of the cotton C-repeat binding factor (CBF) under cold stress condition

Abstract Background Low temperature is a common biological abiotic stress in major cotton growing areas. Cold stress significantly affects the growth, yield and yield quality of cotton. Therefore, it is important to develop a more robust and cold stress tolerant cotton germplasms. In response to climate change and erratic conditions, plants have evolved various survival mechanisms, one of which induction of various stress responsive transcription factors, such as the C-repeat binding factors (CBFs), which have been found to enhance cold tolerance in various plants. Results In this study detailed evaluation of the cotton CBF has been carried out. A total of 29, 28, 25, 21, 30, 26 and 15 proteins encoded by the CBF genes were identified in Gossypium herbaceum, Gossypium arboreum, Gossypium thurberi, Gossypium raimondii, Gossypium turneri, Gossypium longicalyx and Gossypium australe, respectively. Phylogenetic evaluation revealed that the proteins were grouped into seven clades, with clade 1 and 6 being the largest. Moreover, majority of the proteins encoded by the genes were predicted to be located within the nucleus, while some were distributed in other parts of the cell. Based on the transcriptome and RT-qPCR analysis, Gthu17439 (GthCBF4) was highly upregulated under cold stress, and was further validated through forward genetics. The Gthu17439 (GthCBF4) overexpressed plants showed a significantly tolerance to cold stress, with higher germination rate, higher root growth and high induction levels of stress responsive genes. The over-expressed plants exhibited low level of oxidative damage, due to significant reduction in the H2O2 production. Conclusion The results showed that the Gthu17439 (GthCBF4) could be playing a significant role in enhancing cold stress tolerance in cotton and can be further exploited in developing cotton germplasm with an improved cold-stress tolerance

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Functional Characterization of Cotton C-Repeat Binding Factor Genes Reveal Their Potential Role in Cold Stress Tolerance

Frontiers in Plant Science ◽

10.3389/fpls.2021.766130 ◽

2021 ◽

Vol 12 ◽

Author(s):

Jiangna Liu ◽

Richard Odongo Magwanga ◽

Yanchao Xu ◽

Tingting Wei ◽

Joy Nyangasi Kirungu ◽

...

Keyword(s):

Cold Stress ◽

Stress Tolerance ◽

Cell Damage ◽

Germination Rate ◽

Functional Characterization ◽

Growth Yield ◽

Weather Conditions ◽

Forward Genetics ◽

Minimal Cell

Low temperature is a common biological abiotic stress in major cotton-growing areas. Cold stress significantly affects the growth, yield, and yield quality of cotton. Therefore, it is important to develop more robust and cold stress-resilient cotton germplasms. In response to climate change and erratic weather conditions, plants have evolved various survival mechanisms, one of which involves the induction of various stress responsive transcript factors, of which the C-repeat-binding factors (CBFs) have a positive effect in enhancing plants response to cold stress. In this study, genomewide identification and functional characterization of the cotton CBFs were carried out. A total of 29, 28, 25, 21, 30, 26, and 15 proteins encoded by the CBF genes were identified in seven Gossypium species. A phylogenetic evaluation revealed seven clades, with Clades 1 and 6 being the largest. Moreover, the majority of the proteins encoded by the genes were predicted to be located within the nucleus, while some were distributed in other parts of the cell. Based on the transcriptome and RT-qPCR analysis, Gthu17439 (GthCBF4) was highly upregulated and was further validated through forward genetics. The Gthu17439 (GthCBF4) overexpressed plants exhibited significantly higher tolerance to cold stress, as evidenced by the higher germination rate, increased root growth, and high-induction levels of stress-responsive genes. Furthermore, the overexpressed plants under cold stress had significantly reduced oxidative damage due to a reduction in hydrogen peroxide (H2O2) production. Moreover, the overexpressed plants under cold stress had minimal cell damage compared to the wild types, as evidenced by the Trypan and 3,3′-Diaminobenzidine (DAB) staining effect. The results showed that the Gthu17439 (GthCBF4) could be playing a significant role in enhancing cold stress tolerance in cotton and can be further exploited in developing cotton germplasm with improved cold-stress tolerance.

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Genome Wide Identification of Cotton C-Repeat Binding Factor (CBF) and Overexpression of Gthu17439 (GthCBF4) Gene Confer Cold Stress Tolerance in Arabidopsis Thaliana

10.20944/preprints202101.0216.v1 ◽

2021 ◽

Author(s):

Jiangna Liu ◽

Richard Magwanga ◽

Yanchao Xu ◽

Tingting Wei ◽

Jie Zheng ◽

...

Keyword(s):

Cold Stress ◽

Stress Tolerance ◽

Growth Yield ◽

Forward Genetics ◽

Genome Wide ◽

Binding Factor ◽

Growth Vigour ◽

Yield Quality ◽

Clade 1

Low temperature is a common biological abiotic stress in major cotton growing areas. Cold stress significantly affects the growth, yield and yield quality of cotton. Therefore, it is important to develop a more robust and cold stress tolerant cotton germplasms. Climate change and erratic climatic condition, plants have evolved various survival mechanisms, one of which induction of various stress responsive transcriptome factors, such as the C-repeat binding factor GthCBF4, which have been found to enhance cold tolerance in various plants. In this study detailed evaluation of the cotton C-repeat binding factor has been carried out. A total of29, 28, 25, 21, 30, 26 and 15 proteins encoded by the C-repeat binding factor were identified in G. herbaceum, G. arboreum, G. thurberi, G. raimondii, G. turneri, G. longicalyx and G. australe, respectively. Phylogeny evaluation revealed that the proteins were grouped into seven clades, with clade 1 and 6 being the largest. Moreover, majority of the proteins encoded by the genes were predicted to be located within. the nucleus, while some are distributed in other parts of the cell. Based on the transcriptome and RT-qPCR analysis, Gthu17439 (GthCBF4) was highly upregulated and was further validated through forward genetics. The Gthu17439 (GthCBF4) overexpressed plants showed a significantly tolerance to cold stress, with higher growth vigour compared to the wild types. The results showed that the Gthu17439 (GthCBF4) could be playing a significant role in enhancing cold stress tolerance in cotton and can be further exploited in developing a more cold stress tolerance cotton germplasm

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The rice annexin gene OsAnn5 is a positive regulator of cold stress tolerance at the seedling stage

10.21203/rs.3.rs-21726/v1 ◽

2020 ◽

Author(s):

chunxiu shen ◽

Zhiqun Que ◽

Qineng Lu ◽

Tao Liu ◽

Shengqiang Li ◽

...

Keyword(s):

Cold Stress ◽

Stress Tolerance ◽

Stress Responses ◽

Fluorescent Protein ◽

Functional Characterization ◽

Survival Rates ◽

Seedling Stage ◽

Positive Regulator ◽

Annexin Gene ◽

Green Fluorescent

Abstract Annexins exist widely in plants as multigene families and play critical roles in stress responses and a range of cellular processes. In this study, we report on the cloning and functional characterization of the rice annexin gene OsAnn5. We found that the expression of OsAnn5 was induced by cold stress treatment at the seedling stage of rice. GUS staining assay indicated that the expression of OsAnn5 was non tissue-specific and was detected in almost all rice tissues. Subcellular localization indicated that OsAnn5-GFP (green fluorescent protein) signals were found in the endoplasmic reticulum apparatus. Compared with wild type rice, overexpression of OsAnn5 significantly increased survival rates at the seedling stage under cold stress, while knocking out OsAnn5 using the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated proteins) mediated genome editing resulted in sensitivity to cold treatments. These results indicate that OsAnn5 is a positive regulator of cold stress tolerance at the seedling stage.

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Functional characterization of class I SlHSP17.7 gene responsible for tomato cold-stress tolerance

Plant Science ◽

10.1016/j.plantsci.2020.110568 ◽

2020 ◽

Vol 298 ◽

pp. 110568

Author(s):

Ning Zhang ◽

Huaiyin Zhao ◽

Jiewei Shi ◽

Yuanyuan Wu ◽

Jing Jiang

Keyword(s):

Cold Stress ◽

Stress Tolerance ◽

Functional Characterization ◽

Class I

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Comparative transcriptome, physiological and biochemical analyses reveal response mechanism mediated by CBF4 and ICE2 in enhancing cold stress tolerance in Gossypium thurberi

AoB Plants ◽

10.1093/aobpla/plz045 ◽

2019 ◽

Vol 11 (6) ◽

Cited By ~ 1

Author(s):

Xiaoyan Cai ◽

Richard Odongo Magwanga ◽

Yanchao Xu ◽

Zhongli Zhou ◽

Xingxing Wang ◽

...

Keyword(s):

Cold Stress ◽

Stress Tolerance ◽

Cold Tolerance ◽

Molecular Mechanisms ◽

Chilling Stress ◽

Cold Treatment ◽

Cold Sensitivity ◽

Cotton Species ◽

Wide Range ◽

Gossypium Thurberi

Abstract Low temperature is one of the key environmental stresses that impair plant growth and significantly restricts the productivity and spatial distribution of crop plants. Gossypium thurberi, a wild diploid cotton species, has adapted to a wide range of temperatures and exhibits a better tolerance to chilling stress. Here, we compared phenotypes and physiochemical changes in G. thurberi under cold stress and found this species indeed showed better cold tolerance. Therefore, to understand the molecular mechanisms of the cold tolerance in G. thurberi, we compared transcription changes in leaves of G. thurberi under cold stress by high-throughput transcriptome sequencing. In total, 35 617 unigenes were identified in the whole-genome transcription profile, and 4226 differentially expressed genes (DEGs) were discovered in the leaves upon cold treatment. Gene Ontology (GO) classification analyses showed that the majority of DEGs belonged to categories of signal transduction, transcription factors (TFs) and carbohydrate transport and metabolism. The expression of several cold-responsive genes such as ICE1, CBF4, RAP2-7 and abscisic acid (ABA) biosynthesis genes involved in different signalling pathways were induced after G. thurberi seedlings were exposed to cold stress. Furthermore, cold sensitivity was increased in CBF4 and ICE2 virus-induced gene silencing (VIGS) plants, and high level of malondialdehyde (MDA) showed that the CBF4 and ICE2 silenced plants were under oxidative stress compared to their wild types, which relatively had higher levels of antioxidant enzyme activity, as evident by high levels of proline and superoxide dismutase (SOD) content. In conclusion, our findings reveal a new regulatory network of cold stress response in G. thurberi and broaden our understanding of the cold tolerance mechanism in cotton, which might accelerate functional genomics studies and genetic improvement for cold stress tolerance in cultivated cotton.

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Down-Regulation of SlGRAS10 in Tomato Confers Abiotic Stress Tolerance

Genes ◽

10.3390/genes12050623 ◽

2021 ◽

Vol 12 (5) ◽

pp. 623

Author(s):

Sidra Habib ◽

Yee Yee Lwin ◽

Ning Li

Keyword(s):

Salt Stress ◽

Abiotic Stress ◽

Plant Growth ◽

Stress Tolerance ◽

Abiotic Stresses ◽

Abiotic Stress Tolerance ◽

Functional Characterization ◽

Flavonoid Biosynthesis ◽

Ros Scavenging ◽

Down Regulation

Adverse environmental factors like salt stress, drought, and extreme temperatures, cause damage to plant growth, development, and crop yield. GRAS transcription factors (TFs) have numerous functions in biological processes. Some studies have reported that the GRAS protein family plays significant functions in plant growth and development under abiotic stresses. In this study, we demonstrated the functional characterization of a tomato SlGRAS10 gene under abiotic stresses such as salt stress and drought. Down-regulation of SlGRAS10 by RNA interference (RNAi) produced dwarf plants with smaller leaves, internode lengths, and enhanced flavonoid accumulation. We studied the effects of abiotic stresses on RNAi and wild-type (WT) plants. Moreover, SlGRAS10-RNAi plants were more tolerant to abiotic stresses (salt, drought, and Abscisic acid) than the WT plants. Down-regulation of SlGRAS10 significantly enhanced the expressions of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) to reduce the effects of reactive oxygen species (ROS) such as O2− and H2O2. Malondialdehyde (MDA) and proline contents were remarkably high in SlGRAS10-RNAi plants. Furthermore, the expression levels of chlorophyll biosynthesis, flavonoid biosynthesis, and stress-related genes were also enhanced under abiotic stress conditions. Collectively, our conclusions emphasized the significant function of SlGRAS10 as a stress tolerate transcription factor in a certain variety of abiotic stress tolerance by enhancing osmotic potential, flavonoid biosynthesis, and ROS scavenging system in the tomato plant.

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