Functional Characterization of Cotton C-Repeat Binding Factor Genes Reveal Their Potential Role in Cold Stress Tolerance

Low temperature is a common biological abiotic stress in major cotton-growing areas. Cold stress significantly affects the growth, yield, and yield quality of cotton. Therefore, it is important to develop more robust and cold stress-resilient cotton germplasms. In response to climate change and erratic weather conditions, plants have evolved various survival mechanisms, one of which involves the induction of various stress responsive transcript factors, of which the C-repeat-binding factors (CBFs) have a positive effect in enhancing plants response to cold stress. In this study, genomewide identification and functional characterization of the cotton CBFs were carried out. A total of 29, 28, 25, 21, 30, 26, and 15 proteins encoded by the CBF genes were identified in seven Gossypium species. A phylogenetic evaluation revealed seven clades, with Clades 1 and 6 being the largest. Moreover, the majority of the proteins encoded by the genes were predicted to be located within the nucleus, while some were distributed in other parts of the cell. Based on the transcriptome and RT-qPCR analysis, Gthu17439 (GthCBF4) was highly upregulated and was further validated through forward genetics. The Gthu17439 (GthCBF4) overexpressed plants exhibited significantly higher tolerance to cold stress, as evidenced by the higher germination rate, increased root growth, and high-induction levels of stress-responsive genes. Furthermore, the overexpressed plants under cold stress had significantly reduced oxidative damage due to a reduction in hydrogen peroxide (H2O2) production. Moreover, the overexpressed plants under cold stress had minimal cell damage compared to the wild types, as evidenced by the Trypan and 3,3′-Diaminobenzidine (DAB) staining effect. The results showed that the Gthu17439 (GthCBF4) could be playing a significant role in enhancing cold stress tolerance in cotton and can be further exploited in developing cotton germplasm with improved cold-stress tolerance.

Download Full-text

Genome wide identification and functional characterization of the cotton C-repeat binding factor (CBF) under cold stress condition

10.21203/rs.3.rs-439424/v1 ◽

2021 ◽

Author(s):

Jiangna Liu ◽

Richard Magwanga ◽

Yanchao Xu ◽

Tingting Wei ◽

Jie Zheng ◽

...

Keyword(s):

Cold Stress ◽

Stress Tolerance ◽

Germination Rate ◽

Functional Characterization ◽

Growth Yield ◽

Forward Genetics ◽

Gossypium Raimondii ◽

Gossypium Thurberi ◽

Clade 1 ◽

Gossypium Longicalyx

Abstract Background Low temperature is a common biological abiotic stress in major cotton growing areas. Cold stress significantly affects the growth, yield and yield quality of cotton. Therefore, it is important to develop a more robust and cold stress tolerant cotton germplasms. In response to climate change and erratic conditions, plants have evolved various survival mechanisms, one of which induction of various stress responsive transcription factors, such as the C-repeat binding factors (CBFs), which have been found to enhance cold tolerance in various plants. Results In this study detailed evaluation of the cotton CBF has been carried out. A total of 29, 28, 25, 21, 30, 26 and 15 proteins encoded by the CBF genes were identified in Gossypium herbaceum, Gossypium arboreum, Gossypium thurberi, Gossypium raimondii, Gossypium turneri, Gossypium longicalyx and Gossypium australe, respectively. Phylogenetic evaluation revealed that the proteins were grouped into seven clades, with clade 1 and 6 being the largest. Moreover, majority of the proteins encoded by the genes were predicted to be located within the nucleus, while some were distributed in other parts of the cell. Based on the transcriptome and RT-qPCR analysis, Gthu17439 (GthCBF4) was highly upregulated under cold stress, and was further validated through forward genetics. The Gthu17439 (GthCBF4) overexpressed plants showed a significantly tolerance to cold stress, with higher germination rate, higher root growth and high induction levels of stress responsive genes. The over-expressed plants exhibited low level of oxidative damage, due to significant reduction in the H2O2 production. Conclusion The results showed that the Gthu17439 (GthCBF4) could be playing a significant role in enhancing cold stress tolerance in cotton and can be further exploited in developing cotton germplasm with an improved cold-stress tolerance

Download Full-text

Functional characterization of class I SlHSP17.7 gene responsible for tomato cold-stress tolerance

Plant Science ◽

10.1016/j.plantsci.2020.110568 ◽

2020 ◽

Vol 298 ◽

pp. 110568

Author(s):

Ning Zhang ◽

Huaiyin Zhao ◽

Jiewei Shi ◽

Yuanyuan Wu ◽

Jing Jiang

Keyword(s):

Cold Stress ◽

Stress Tolerance ◽

Functional Characterization ◽

Class I

Download Full-text

Genome Wide Identification of Cotton C-Repeat Binding Factor (CBF) and Overexpression of Gthu17439 (GthCBF4) Gene Confer Cold Stress Tolerance in Arabidopsis Thaliana

10.20944/preprints202101.0216.v1 ◽

2021 ◽

Author(s):

Jiangna Liu ◽

Richard Magwanga ◽

Yanchao Xu ◽

Tingting Wei ◽

Jie Zheng ◽

...

Keyword(s):

Cold Stress ◽

Stress Tolerance ◽

Growth Yield ◽

Forward Genetics ◽

Genome Wide ◽

Binding Factor ◽

Growth Vigour ◽

Yield Quality ◽

Clade 1

Low temperature is a common biological abiotic stress in major cotton growing areas. Cold stress significantly affects the growth, yield and yield quality of cotton. Therefore, it is important to develop a more robust and cold stress tolerant cotton germplasms. Climate change and erratic climatic condition, plants have evolved various survival mechanisms, one of which induction of various stress responsive transcriptome factors, such as the C-repeat binding factor GthCBF4, which have been found to enhance cold tolerance in various plants. In this study detailed evaluation of the cotton C-repeat binding factor has been carried out. A total of29, 28, 25, 21, 30, 26 and 15 proteins encoded by the C-repeat binding factor were identified in G. herbaceum, G. arboreum, G. thurberi, G. raimondii, G. turneri, G. longicalyx and G. australe, respectively. Phylogeny evaluation revealed that the proteins were grouped into seven clades, with clade 1 and 6 being the largest. Moreover, majority of the proteins encoded by the genes were predicted to be located within. the nucleus, while some are distributed in other parts of the cell. Based on the transcriptome and RT-qPCR analysis, Gthu17439 (GthCBF4) was highly upregulated and was further validated through forward genetics. The Gthu17439 (GthCBF4) overexpressed plants showed a significantly tolerance to cold stress, with higher growth vigour compared to the wild types. The results showed that the Gthu17439 (GthCBF4) could be playing a significant role in enhancing cold stress tolerance in cotton and can be further exploited in developing a more cold stress tolerance cotton germplasm

Download Full-text

Identification and functional characterization of Siberian wild rye (Elymus sibiricus L.) small heat shock protein 16.9 gene (EsHsp16.9) conferring diverse stress tolerance in prokaryotic cells

Biotechnology Letters ◽

10.1007/s10529-014-1749-1 ◽

2014 ◽

Vol 37 (4) ◽

pp. 881-890 ◽

Cited By ~ 9

Author(s):

Sang-Hoon Lee ◽

Ki-Won Lee ◽

Dong-Gi Lee ◽

Daeyoung Son ◽

Su Jung Park ◽

...

Keyword(s):

Heat Shock ◽

Heat Shock Protein ◽

Stress Tolerance ◽

Functional Characterization ◽

Small Heat Shock Protein ◽

Elymus Sibiricus

Download Full-text

Functional Characterization of Transcription Factor Win1 Genes Associated with Oil Biosynthesis and Stress Tolerance in Soybean (Glycine Max)

SSRN Electronic Journal ◽

10.2139/ssrn.3985224 ◽

2021 ◽

Author(s):

Guiping Cai ◽

Baoling Liu ◽

Yali Zhou ◽

Huiling Gao ◽

Jinai Xue ◽

...

Keyword(s):

Transcription Factor ◽

Glycine Max ◽

Stress Tolerance ◽

Functional Characterization ◽

Oil Biosynthesis

Download Full-text

Functional characterization of Gh_A08G1120 (GH3.5) gene reveal their significant role in enhancing drought and salt stress tolerance in cotton

BMC Genetics ◽

10.1186/s12863-019-0756-6 ◽

2019 ◽

Vol 20 (1) ◽

Cited By ~ 2

Author(s):

Joy Nyangasi Kirungu ◽

Richard Odongo Magwanga ◽

Pu Lu ◽

Xiaoyan Cai ◽

Zhongli Zhou ◽

...

Keyword(s):

Salt Stress ◽

Stress Tolerance ◽

Significant Role ◽

Functional Characterization ◽

Salt Stress Tolerance ◽

Drought And Salt Stress

Download Full-text

From model to crop: functional characterization of SPL8 in M. truncatula led to genetic improvement of biomass yield and abiotic stress tolerance in alfalfa

Plant Biotechnology Journal ◽

10.1111/pbi.12841 ◽

2017 ◽

Vol 16 (4) ◽

pp. 951-962 ◽

Cited By ~ 20

Author(s):

Jiqing Gou ◽

Smriti Debnath ◽

Liang Sun ◽

Amy Flanagan ◽

Yuhong Tang ◽

...

Keyword(s):

Abiotic Stress ◽

Stress Tolerance ◽

Genetic Improvement ◽

Biomass Yield ◽

Abiotic Stress Tolerance ◽

Functional Characterization

Download Full-text

The rice annexin gene OsAnn5 is a positive regulator of cold stress tolerance at the seedling stage

10.21203/rs.3.rs-21726/v1 ◽

2020 ◽

Author(s):

chunxiu shen ◽

Zhiqun Que ◽

Qineng Lu ◽

Tao Liu ◽

Shengqiang Li ◽

...

Keyword(s):

Cold Stress ◽

Stress Tolerance ◽

Stress Responses ◽

Fluorescent Protein ◽

Functional Characterization ◽

Survival Rates ◽

Seedling Stage ◽

Positive Regulator ◽

Annexin Gene ◽

Green Fluorescent

Abstract Annexins exist widely in plants as multigene families and play critical roles in stress responses and a range of cellular processes. In this study, we report on the cloning and functional characterization of the rice annexin gene OsAnn5. We found that the expression of OsAnn5 was induced by cold stress treatment at the seedling stage of rice. GUS staining assay indicated that the expression of OsAnn5 was non tissue-specific and was detected in almost all rice tissues. Subcellular localization indicated that OsAnn5-GFP (green fluorescent protein) signals were found in the endoplasmic reticulum apparatus. Compared with wild type rice, overexpression of OsAnn5 significantly increased survival rates at the seedling stage under cold stress, while knocking out OsAnn5 using the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated proteins) mediated genome editing resulted in sensitivity to cold treatments. These results indicate that OsAnn5 is a positive regulator of cold stress tolerance at the seedling stage.

Download Full-text