scholarly journals Circulating Isolates of Neisseria mucosa do not Inhibit the Growth of Neisseria gonorrhoeae

2021 ◽  
Author(s):  
Saïd Abdellati ◽  
Jolein Laumen ◽  
Natalia Gonzalez ◽  
Sheeba Basil ◽  
Christophe Van Dijck ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Streptococcus Pneumoniae ◽  
Neisseria Gonorrhoeae ◽  
Inhibitory Effect ◽  
Neisseria Mucosa ◽  
Strong Inhibitory Effect ◽  
Positive Controls

We used agar overlay assays to assess if 24 circulating and historical isolates of Neisseria mucosa could inhibit the growth of 28 circulating and historical isolates of N. gonorrhoeae. We found no evidence of inhibition by N. mucosa (n=24). Positive controls Streptococcus pneumoniae and Escherichia coli demonstrated a strong inhibitory effect against the growth of N. gonorrhoeae.

scholarly journals Neisseria Mucosa Does Not Inhibit the Growth of Neisseria gonorrhoeae

2021 ◽  
Author(s):  
Said Abdellati ◽  
Jolein Laumen ◽  
Natalia Gonzalez ◽  
Sheeba Manoharan-Basil ◽  
Christophe Van Dijck ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Streptococcus Pneumoniae ◽  
Neisseria Gonorrhoeae ◽  
Inhibitory Effect ◽  
Optical Illusion ◽  
Neisseria Species ◽  
Neisseria Mucosa ◽  
Strong Inhibitory Effect ◽  
Commensal Neisseria ◽  
Positive Controls

Antibiotic-sparing treatments are required to prevent the further emergence of anti-microbial resistance in Neisseria gonorrhoeae. Commensal Neisseria species have previously been found to inhibit the growth of pathogenic Neisseria species. For example, a previous study found that 3 out of 5 historical isolates of Neisseria mucosa could inhibit the growth of N. gonorrhoeae. In this study, we used agar overlay assays to assess if 24 circulating and historical isolates of Neisseria mucosa could inhibit the growth of 28 circulating and historical isolates of N. gonorrhoeae. Although pitting around each colony of N. mucosa created an optical illusion of decreased growth of N. gonorrhoeae, we found no evidence of inhibition (n=24). In contrast, positive controls of Streptococcus pneumoniae and Escherichia coli demonstrated a strong inhibitory effect against the growth of N. gonorrhoeae.

scholarly journals Study on the extraction and antibacterial activity of Monascin

2021 ◽  
Vol 251 ◽  
pp. 02061
Author(s):  
Xiaojuan Gao ◽  
Xiaoshi Lu ◽  
Zifeng Wang ◽  
Guangpeng Liu ◽  
Xinjun Li
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Aspergillus Niger ◽  
Inhibitory Effect ◽  
Ethanol Extraction ◽  
Inhibitory Ability ◽  
Strong Inhibitory Effect

Taking monascin as the research object, monascin was extracted from red kojic rice by ethanol extraction and extracted with 60%, 70% and 80% ethanol respectively. Finally, it was concluded that when the concentration of ethanol was 70%, the extraction rate of monascin was the highest, reached 75.68%. The bacteriostatic experiments of monascin extract and monascin fermentation showed that it had strong inhibitory effect on Staphylococcus aureus and Bacillus subtilis, weak inhibitory ability on Escherichia coli and Aspergillus niger, and no obvious inhibitory effect on the growth of Saccharomyces cerevisiae.

scholarly journals Identification of the Conjugative mefGene in Clinical Acinetobacter junii and Neisseria gonorrhoeae Isolates

2000 ◽  
Vol 44 (9) ◽  
pp. 2503-2506 ◽  
Author(s):  
Vicki A. Luna ◽  
Sydney Cousin ◽  
William L. H. Whittington ◽  
Marilyn C. Roberts
Keyword(s):  
Streptococcus Pneumoniae ◽  
Neisseria Gonorrhoeae ◽  
Moraxella Catarrhalis ◽  
Efflux Pump ◽  
Eikenella Corrodens ◽  
Gram Positive ◽  
Gram Negative ◽  
Acinetobacter Junii ◽  
Neisseria Mucosa ◽  
Neisseria Perflava

ABSTRACT The mef gene, originally described for gram-positive organisms and coding for an efflux pump, has been identified in clinical isolates of Acinetobacter junii andNeisseria gonorrhoeae. These strains could transfer themef gene at frequencies ranging from 10−6 to 10−9 into one or more of the following recipients: gram-negative Moraxella catarrhalis, Neisseria perflava/sicca and Neisseria mucosa and gram-positiveEnterococcus faecalis. Three Streptococcus pneumoniae strains could transfer the mef gene intoEikenella corrodens, Haemophilus influenzae,Kingella denitrificans, M. catarrhalis,Neisseria meningitidis, N. perflava/sicca, andN. mucosa at similar frequencies. The mef gene can thus be transferred to and expressed in a variety of gram-negative recipients.

scholarly journals The Effect of Environmental Factors on Microbial Growth

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Rhezqy Furwati Jufri
Keyword(s):  
Escherichia Coli ◽  
Environmental Factors ◽  
Microbial Growth ◽  
Inhibitory Effect ◽  
Tetracycline Antibiotics ◽  
Experimental Procedure ◽  
E Coli ◽  
Chemical Factors ◽  
Strong Inhibitory Effect ◽  
Escherichia Coli Bacteria

Chemical factors that have inhibition based on the results of this practicum are Tetracycline and formalin. In this experiment, no type of liquid bath soap was found that inhibited the growth of E. coli. This can occur because of an error in the experimental procedure. Escherichia coli bacteria can grow optimally in the temperature range of 30 ° C. Formalin has a strong inhibitory effect on the growth of Escherichia coli bacteria, as well as Tetracycline antibiotics which can inhibit the growth of E. coli bacteria.

scholarly journals Developing Lactic Acid Bacteria as an Oral Healthy Food

Life ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 268
Author(s):  
Wei-Kuang Lai ◽  
Ying-Chen Lu ◽  
Chun-Ren Hsieh ◽  
Chien-Kei Wei ◽  
Yi-Hong Tsai ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Inhibitory Effect ◽  
Periodontal Pathogen ◽  
Periodontal Pathogens ◽  
Health Food ◽  
Pocket Depth ◽  
Oral Tablet ◽  
Strong Inhibitory Effect ◽  
Oral Pathogens

Lactic acid bacteria have functions in immunoregulation, antagonism, and pathogen inhibition. The purpose of this study was to evaluate the effectiveness of lactic acid bacteria (LAB) in countering oral pathogens and develop related products. After a series of assays to 450 LAB strains, 8 heat-inactivated strains showed a strong inhibitory effect on a caries pathogen, Streptococcus mutans, and 308 heat-inactivated LAB strains showed a strong inhibitory effect on a periodontal pathogen, Porphyromonas gingivalis. The key reasons for inhibiting oral pathogens were bacteriocins produced by LAB and the coaggregation effect of the inactivated cells. We selected Lacticaseibacillus (Lb) paracasei 111 and Lb.paracasei 141, which had the strongest inhibitory effects on the above pathogens, was the main oral health food source. The optimal cultural conditions of Lb. paracasei 111 and Lb. paracasei 141 were studied. An oral tablet with a shelf life of 446 days made of the above strains was developed. A 40 volunteers’ clinical study (CSMUH IRB number: CS05065) was conducted with this tablet in the Periodontological Department of the Stomatology Research Center, Affiliated Hospital of Chung Shan Medical University (Taiwan). After 8 weeks of testing, 95% and 78.9% of patients showed an effect on reducing periodontal pathogens and improving probing pocket depth, respectively, in the oral tablet group.

scholarly journals Mosaic-Like Structure of Penicillin-Binding Protein 2 Gene (penA) in Clinical Isolates of Neisseria gonorrhoeae with Reduced Susceptibility to Cefixime

2002 ◽  
Vol 46 (12) ◽  
pp. 3744-3749 ◽  
Author(s):  
Satoshi Ameyama ◽  
Shoichi Onodera ◽  
Masahiro Takahata ◽  
Shinzaburo Minami ◽  
Nobuko Maki ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Neisseria Gonorrhoeae ◽  
Neisseria Meningitidis ◽  
Binding Protein ◽  
Clinical Isolates ◽  
Penicillin Binding Protein ◽  
Gene Sequences ◽  
Neisseria Flavescens ◽  
Neisseria Perflava

ABSTRACT Neisseria gonorrhoeae strains with reduced susceptibility to cefixime (MICs, 0.25 to 0.5 μg/ml) were isolated from male urethritis patients in Tokyo, Japan, in 2000 and 2001. The resistance to cephems including cefixime and penicillin was transferred to a susceptible recipient, N. gonorrhoeae ATCC 19424, by transformation of the penicillin-binding protein 2 gene (penA) that had been amplified by PCR from a strain with reduced susceptibility to cefixime (MIC, 0.5 μg/ml). The sequences of penA in the strains with reduced susceptibilities to cefixime were different from those of other susceptible isolates and did not correspond to the reported N. gonorrhoeae penA gene sequences. Some regions in the transpeptidase-encoding domain in this penA gene were similar to those in the penA genes of Neisseria perflava (N. sicca), Neisseria cinerea, Neisseria flavescens, and Neisseria meningitidis. These results showed that a mosaic-like structure in the penA gene conferred reductions in the levels of susceptibility of N. gonorrhoeae to cephems and penicillin in a manner similar to that found for N. meningitidis and Streptococcus pneumoniae.

Evaluation of FCE 22101 in experimental meningitis caused by Escherichia coli and Streptococcus pneumoniae

1989 ◽  
Vol 23 (suppl C) ◽  
pp. 143-148
Author(s):  
R. J. Dworkin ◽  
J. H. Tureen ◽  
S. L. Kennedy ◽  
M. Sachdeva ◽  
M. A. Sande
Keyword(s):  
Escherichia Coli ◽  
Streptococcus Pneumoniae

scholarly journals Resistance of Streptococcus pneumoniaeto Deformylase Inhibitors Is Due to Mutations indefB

2001 ◽  
Vol 45 (9) ◽  
pp. 2432-2435 ◽  
Author(s):  
Peter Margolis ◽  
Corinne Hackbarth ◽  
Sara Lopez ◽  
Mita Maniar ◽  
Wen Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Streptococcus Pneumoniae ◽  
Peptide Deformylase ◽  
Wild Type ◽  
Resistance Phenotype ◽  
Resistant Mutants

ABSTRACT Resistance to peptide deformylase inhibitors in Escherichia coli or Staphylococcus aureus is due to inactivation of transformylase activity. Knockout experiments in Streptococcus pneumoniae R6x indicate that the transformylase (fmt) and deformylase (defB) genes are essential and that adef paralog (defA) is not. Actinonin-resistant mutants of S. pneumoniae ATCC 49619 harbor mutations indefB but not in fmt. Reintroduction of the mutated defB gene into wild-type S. pneumoniaeR6x recreates the resistance phenotype. The altered enzyme displays decreased sensitivity to actinonin.

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