scholarly journals Evaluation of the Diagnostic Values and Utility of Helicobacter Pylori Stool Antigen Lateral Immunochromatography Assay

2021 ◽  
Author(s):  
Shaymaa Abdelmalek ◽  
Wafy Hamed ◽  
Neven Nagy ◽  
Karim Shokry ◽  
Hisham Abdelrahman
Keyword(s):  
Helicobacter Pylori ◽  
Age Groups ◽  
Cost Effective ◽  
Chi Square ◽  
Non Invasive ◽  
Gender And Age ◽  
Confirmatory Tests ◽  
Diagnostic Values ◽  
Stool Antigen ◽  
H Pylori

Helicobacter pylori is the most common human gastric infection. H. pylori stool antigen lateral flow immunochromatography assay (HpSA-LFIA) is considered one of the most cost-effective and rapid non-invasive assays (active tests). The evaluation of this test is crucial for accuracy and utility assurance. This study aimed to evaluate the polyclonal antibody-based HpSA-LFIA in comparison to a monoclonal antibody-based ELISA kit. Methodology: Stool samples were collected from 200 gastric patients for HpSA-LFIA and semi-quantitative HpSA-ELISA. Statistical analysis of the diagnostic values was performed using MedCalc software. Chi-square tests were used to determine the effects of gender and age. Results: The obtained results found that HpSA-LFIA achieved promising sensitivity (93.75%) and NPV (98.00%). However, it had poor specificity, PPV, and accuracy, respectively, 59.76%, 31.25%, and 65.31%. LR+ & LR- were 2.33% & 0.1%, respectively. Gender had no significance on the di-agnostic parameters of HpSA-LFIA. Age groups had irrelevant sensitivity; however, specificity was significantly higher in patients over 45 years. Conclusion: It was concluded that HpSA-LFIA was not accurate enough to be the sole test for di-agnosis and needs other confirmatory tests in case of positive conditions

scholarly journals Evaluation of a stool antigen test using a mAb for native catalase for diagnosis of Helicobacter pylori infection in children and adults

2014 ◽  
Vol 63 (12) ◽  
pp. 1621-1625 ◽  
Author(s):  
Masumi Okuda ◽  
Takako Osaki ◽  
Shogo Kikuchi ◽  
Junko Ueda ◽  
Yingsong Lin ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Real Time ◽  
Real Time Pcr ◽  
High Specificity ◽  
Antigen Test ◽  
Stool Antigen Test ◽  
Non Invasive ◽  
Significant Difference ◽  
Stool Antigen ◽  
H Pylori

Non-invasive diagnosis of Helicobacter pylori infection is important not only for screening of infection but also for epidemiological studies. Stool antigen tests are non-invasive and are convenient to identify H. pylori infection, particularly in children. We evaluated the stool antigen test, which uses a mAb for native catalase of H. pylori developed in Japan. A total of 151 stool samples were collected from participants (52 children and 99 adults) of the Sasayama Cohort Study and stored between −30 and −80 °C. The stool antigen test used was Testmate pylori antigen (TPAg), and was performed according to the manufacturer’s instructions. Furthermore, we conducted a quantitative real-time PCR test and compared the PCR results with those of the TPAg test. When compared with the results in real-time PCR, the sensitivity of TPAg was 89.5 % overall, 82.7 % for children and 92.4 % for adults, and the specificity was 100 %. The accuracy was 93.4 % overall, 90.4 % for children and 94.9 % for adults, and there was no significant difference in the accuracy of TPAg between children and adults. Five of 28 children (18 %) and five of 38 adults (13 %) were PCR positive with negative TPAg results. Four of five children with positive PCR and negative TPAg results were given a 13C-urea breath test and all four children tested negative. No significant correlation was observed between the TPAg results and DNA numbers of H. pylori in faeces among children or adults. A stool antigen test (TPAg) using a mAb for native catalase is useful for diagnosis of H. pylori in children and adults. Additionally, this test has particularly high specificity.

scholarly journals Retrospective Evaluation of Helicobacter pylori Antigen Positivity in the Stool Samples of Patients with Gastroduodenal Complaints

2021 ◽  
Author(s):  
Tevhide Ziver Sarp ◽  
Harika Öykü Dinç ◽  
Doğukan Özbey ◽  
Seher Akkuş ◽  
Beyza Aslan ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Age Groups ◽  
Test Results ◽  
Stool Antigen Test ◽  
Cerrahpasa Medical Faculty ◽  
Study Results ◽  
Stool Antigen ◽  
Stool Samples ◽  
H Pylori ◽  
Age Range

Objective: Helicobacter pylori is an important pathogen that causes the development of important gastroduodenal pathologies such as acute and chronic gastritis, ulcer and gastric cancer. This pathogen is estimated to infect about half of the world’s population. In this study, it was aimed to evaluate the five-year data retrospectively by investigating the presence of H. pylori antigen in stool samples taken from patients who applied to Istanbul University-Cerrahpaşa Medical Faculty Medical Microbiology Department Serology/ELISA laboratory with gastroduodenal complaints between January 2015 and December 2019. Method: Stool specimens of 4696 patients admitted to the hospital with gastroduodenal complaints between January 2015 and December 2019 were analyzed using an immunochromotographic H. pylori stool antigen test containing monoclonal antibodies. Results: Among 4696 patients who were examined retrospectively in a five-year period, 1176 (25%) had positive, and 3520 (75%) of them negative H. pylori test results. H. pylori-positivity was found in 210 (21.30%) of 986 children and 966 (26.04%) of 3710 adults. H. pylori detection rate in children was found to be significantly lower than adults (p: 0.002). H. pylori positivity was found higher in the 30-39 age range compared to other age groups. Conclusion: Although current study results showed a lower prevalence compared to recent studies, it has been concluded that H. pylori is an important pathogen in all age groups with gastroduodenal complaints and should be monitored in the community.

scholarly journals Efficacy of Different Laboratory Tests to Diagnose Helicobacter pylori Infection

2013 ◽  
Vol 8 (1) ◽  
pp. 11-14 ◽  
Author(s):  
MM Shahin Ul Islam ◽  
Shamsun Nahar ◽  
Mst Naznin Sarker ◽  
ASM Salimullah ◽  
Mohammad Asadur Rahman ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Positive Predictive Value ◽  
Negative Predictive Value ◽  
Predictive Value ◽  
Laboratory Tests ◽  
Antigen Test ◽  
Stool Antigen Test ◽  
Non Invasive ◽  
Stool Antigen ◽  
H Pylori

Helicobacter pylori is a Gram negative bacteria which causes chronic gastritis, peptic ulcer disease, primary B-cell gastric lymphoma, and adenocarcinoma of the stomach. There are a set of laboratory tests to diagnose H. pylori infection with a variable accuracy, they are divided into non-invasive tests and invasive tests. Non-invasive tests include serology, urea breath test (UBT) and stool antigen test (SAT). Invasive tests include rapid urease test (RUT), histology and culture. This cross sectional study was carried out in the Department of Gastroenterology, Bangabandhu Sheikh Mujib Medical University (BSMMU) and H. pylori laboratory of International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B) from July 2008 to September 2009 to evaluate the efficacy of RUT, SAT and Culture as a diagnostic tool for H. pylori. Dyspeptic patients were collected from outpatient department of BSMMU. Out of 224 dyspeptic patients 149 patients had ulcers or erosions in the stomach or duodenum. Stool sample could be collected from 139 patients. RUT has sensitivity of 100%, specificity 80.28%, positive predictive value 85% and negative predictive value 100%. Regarding culture, sensitivity is 100%, specificity 94.37%, positive predictive value 95% and negative predictive value 100%. Stool antigen test has sensitivity 95.94%, specificity 92.31%,positive predictive value 93% and negative predictive value 95%. DOI: http://dx.doi.org/10.3329/fmcj.v8i1.16890 Faridpur Med. Coll. J. 2013;8(1): 11-14

scholarly journals Evaluation of Rapid stool antigen test for the diagnosis of Helicobacter pylori infection in patients with dyspepsia

2017 ◽  
Vol 10 (2) ◽  
pp. 39-44
Author(s):  
Salma Khatun ◽  
Fahmida Rahman ◽  
Khandaker Shadia ◽  
Indrajit Kumar Dutta ◽  
Mohammad Nazmul Hoq ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Peptic Ulcer ◽  
Helicobacter Pylori Infection ◽  
Adult Patients ◽  
Antigen Test ◽  
Stool Antigen Test ◽  
Non Invasive ◽  
Stool Antigen ◽  
Stool Samples ◽  
H Pylori

Background and objectives: Several diagnostic assays are used for the detection of Helicobacter pylori infection in suspected peptic ulcer cases. H. pylori stool antigen test is a non-invasive method for the detection of active infection. The present study has evaluated the efficacy of rapid stool antigen test to diagnose H. pylori infection in patients with dyspepsia.Materials and methods: Adult patients with complains of dyspepsia attending the Department of Gastroenterology, Hepatobiliary and Pancreatic Diseases (GHPD) of BIRDEM hospital for endoscopy were included. Gastric biopsy, blood and stool samples were obtained from each participant after informed written consent. Rapid urease test (RUT), serum H. pylori immunoglobulin A (IgA) and IgG and rapid H. pylori stool antigen (HpSAg) tests were performed. Only stool samples were obtained from 31 neonates aged 1 to 30 days as negative control for HpSAg test.Results: A total of 91 adult patients with complain of dyspepsia were included in the study. Out of 91 cases, 17 (18.7%) and 74 (81.3%) had peptic ulcer and erosion respectively. HpSAg was positive in 63.7% cases compared to 42.9% and 62.6% respectively by RUT and IgA. The rate of HpSAg positivity was significantly higher (p<0.05) in ulcer compared to erosion cases. HpSAg test was positive in all (100%) RUT positive cases. Combination of HpSAg test and IgA yielded highest positive result in both ulcer (82.4%) and erosion (84%) cases. H. pylori IgG was positive in all cases.Conclusion: The study has demonstrated that HpSAg test is an effective and non-invasive diagnostic tool to detect active H. pylori infection in suspected dyspeptic patients.IMC J Med Sci 2016; 10(2): 39-44

scholarly journals Evaluation the Efficacy of ELISA IgG, IgM and IgA Tests for Diagnosis of Helicobacter pylori

2018 ◽  
pp. 172-176
Author(s):  
Hogir Mohammed Shukri Saadi ◽  
Ali Yahya Saeed
Keyword(s):  
Helicobacter Pylori ◽  
Predictive Value ◽  
Serological Test ◽  
Smoking Status ◽  
Age Groups ◽  
Laboratory Diagnosis ◽  
Serological Tests ◽  
Gold Standard Method ◽  
Non Invasive ◽  
H Pylori

 It has been well recognized throughout the world that Helicobacter pylori is the main cause of gastric ulcer and stomach carcinoma. Laboratory diagnosis of H. pylori infection is made by invasive and non-invasive methods. Invasive methods require endoscopy which is uncomfortable an unacceptable by the most patients. Therefore, non-invasive methods particularly serological tests are easier and comfortable for patients.  A total of 86 patients with ages ranging from 18-77 years old (43 males and 43 females) who were referred to the Duhok Hepatology & Gastroenterology center/Azadi Teaching Hospital for endoscopic examination from June to October, 2013 were enrolled in the study. From each patient 5 ml of blood was collected under a septic condition and sera were separated for serology. Data from each participant were recorded in a special questionnaire form after consent agreed upon on ethical and scientific committee of the Azadi hospital. The efficacy of three different ELISA tests (IgG, IgM, IgA) was assessed taking culture as a gold standard method. pylori was found in 70.93%, 30.23% and 5.81% by ELISA IgG, IgA and IgM tests respectively. The highest percentage (70.93%) of H. pylori positive cases were found by ELISA IgG and the lowest percentage (5.81%) was recorded in ELISA IgM. The study found a significant relationship between age groups and H. pylori positive cases by ELISA IgG in which positive cases increased with the increase of ages. No statistical correlation was found between the sex, smoking status and residency of studied patients with H. pylori infection. A significant correlation was found between patients without endoscopic lesions and positive cases for H. pylori by ELISA IgG in which 75.67% of patients without endoscopic lesions were positive for anti-H. pylori antibodies compared to 41.66% in patients with endoscopic lesions. The efficacy of IgG, IgM and IgA tests was calculated compared with golden standard tests and ELISA IgG characterized by the highest values of sensitivity (75.75%), negative predictive value (36.00%) and accuracy (86.00%) but with lowest specificity (45.00%) and similar positive predictive value (81.96%) with IgM and IgA. The lowest sensitivity was 5.97% and accuracy 25.56% with ELISA Ig M. From the results of the present study we can conclude that among ELISA tests, anti-H. pylori IgG is a reliable serological test for the diagnosis of H. pylori infection.

scholarly journals Non-invasive stool antigen test for screening of Helicobacter pylori infection and assessing efficacy of treatment in patients with peptic ulcer

2013 ◽  
Vol 2 (1) ◽  
pp. 28-33
Author(s):  
Rezina Karim ◽  
SM Moslehuddin Ahmed ◽  
Fahmida Begum
Keyword(s):  
Helicobacter Pylori ◽  
Peptic Ulcer ◽  
Helicobacter Pylori Infection ◽  
World Population ◽  
Antigen Test ◽  
Stool Antigen Test ◽  
Non Invasive ◽  
Stool Antigen ◽  
H Pylori ◽  
Efficacy Of Treatment

Helicobacter pylori infection is one of the most common infections in humans, with an estimated 50% of the world population being infected.  The infection is strongly associated with chronic gastritis, peptic ulcer, adenocarcinoma and non-Hodgkin’s lymphoma of stomach. The prevalence of infection is high in developing countries, demanding a reliable diagnostic and treatment method. The present study was designed to investigate the monoclonal antibody-based H. Pylori stool antigen test to screen H. pylori infection and assess efficacy of treatment in patients with peptic ulcer. A total of 89 patients who underwent upper gastrointestinal endoscopy from July 2007 to June 2008 at Bangabandhu Sheikh Mujib Medical University Hospital, Dhaka, Bangladesh were included in the study. Endoscopic findings showed that out of 89 patients, 54 (60.7%) had duodenal ulcers, 24 (27%) had antral erosion and 5 (5.6%) had gastric ulcers. With RUT (rapid urease test) and histopathology of biopsy samples of 89 patients, 78 (87.6%) patients were found to be H. pylori positive. Stool antigen test was positive in 72 (92.3%) out of 78 H. pylori positive patients. The monoclonal stool antigen test (SAT) revealed 92.3% sensitivity and specificity of 100% before treatment. Among 52 follow-up patients (after treatment), 5 (9.6%) patients were detected positive by histology, RUT and stool antigen test, and 35 (67.3%) patients were negative by 3 tests. So the monoclonal SAT revealed 100% sensitivity and 100% specificity after treatment. The monoclonal stool antigen test is highly sensitive and a specific tool for diagnosis of H. pylori infection before therapy and can assess the success of eradication after therapy. It also offers the advantage of specificity and reliability over the invasive test.  It is easy and quick to use, non-invasive and does not require any special technology.South East Asia J Public Health | Jan-June 2012 | Vol 2 Issue 1 | 28-33 DOI: http://dx.doi.org/10.3329/seajph.v2i1.15262

Perfil Epidemiológico de Pacientes Submetidos à Biópsia Gástrica em um Hospital Escola do Sul de Minas Gerais/ Epidemiological Profile of Patients Undergo Gastric Biopsy in a School Hospital of Minas Gerais

1970 ◽  
Vol 4 (3) ◽  
pp. 48-57
Author(s):  
Isabela Maria A. Ribeiro Simões ◽  
Ana Carolina Mauad Coli ◽  
Roseane de Souza Candido Irulegui
Keyword(s):  
Helicobacter Pylori ◽  
Age Groups ◽  
Epidemiological Data ◽  
Minas Gerais ◽  
Gastric Biopsy ◽  
Gastric Ulcers ◽  
Histopathological Diagnosis ◽  
Gastric Diseases ◽  
H Pylori ◽  
Epidemiological Profile

Objetivo: Determinar a prevalência de lesões benignas e neoplasia gástrica através do estudo de biópsias realizadas em um Hospital Escola do Sul de Minas Gerais, no período entre 2007 e 2011. Materiais e Métodos: A pesquisa documental foi quantitativa e retrospectiva, baseada na análise dos registros de biópsias e prontuários. Realizou-se o levantamento de dados referentes à idade, gênero, cor, profissão, diagnóstico histopatológico e presença de Helicobacter pylori nas amostras. Resultados: O número total de biópsias gástricas analisadas foi de 1225, cujo perfil populacional encontrado foi: idade média de 56,75 anos, sexo masculino (52%), cor branca (81,9 %), aposentado (30%). Os diagnósticos mais frequentes foram: gastrites (71,9%), pólipos (14,2%), adenocarcinomas (5,9%), úlceras gástricas (6%), linfomas (0,4%), sem alterações (0,4%) e outros (1,2%). Em outros, encontram-se achados de malignidade, metaplasia e xantelasma gástrico. Em relação à presença de Helicobacter pylori nas amostras, o resultado encontrado foi de24% positivas, 46% negativas e 30% não pesquisadas. Conclusão: Os resultados confirmam a alta frequência das doenças gástricas e sua incidência nas diversas faixas etárias, além do envolvimento do H. pylori em tais afecções. É de grande importância a caracterização dos dados epidemiológicos, o que permite prováveis direcionamentos para programas de prevenção e informação para a população. Palavras-chave: biópsia gástrica, gastropatia, perfil epidemiológico.  ABSTRACTObjective: To determine the prevalence of benign lesions and gastric cancer through study of biopsies performed at a school hospital in southern Minas Gerais, in the period between 2007 and 2011.Materials and Methods: The research was quantitative and retrospective, based on analysis of biopsies records and medical records. We conducted the survey data regarding age, sex, color, profession, histopathological diagnosis and the presence of Helicobacter pylori in the samples. Results: The total number of gastric biopsies analyzed was 1225. Population listing was found: mean age of 56.75 years, male (52%), white (81.9%), retired (30%). The most frequent diagnoses were gastritis (71.9%), polyps (14.2%), adenocarcinomas (5.9%), gastric ulcers (6%), lymphoma (0.4%), unchanged (0, 4%) and others (1.2%). In others, there are: findings of malignancy, metaplasia, gastric xanthelasma. Regarding the presence of Helicobacter pylori in the sample, the result was: 24% positive, 46% negative, 30% non searched. Conclusion: The results confirm the high frequency of gastric diseases and their incidence in the various age groups additionally to the involvement of H. pylori in such conditions. It is of great importance to characterize the epidemiological data, allowing probable directions for prevention and information programs for population. Keywords: gastric biopsy, gastropathy, epidemiological profile

Endoscopic Biopsy Pathology of Helicobacter pylori Gastritis

1999 ◽  
Vol 123 (9) ◽  
pp. 778-781 ◽  
Author(s):  
Maher Toulaymat ◽  
Sharon Marconi ◽  
Jane Garb ◽  
Christopher Otis ◽  
Shirin Nash
Keyword(s):  
Helicobacter Pylori ◽  
Specific Antibody ◽  
Cost Effective ◽  
Endoscopic Biopsy ◽  
Immunohistochemical Method ◽  
Labor Costs ◽  
Chronic Active Gastritis ◽  
Cost Effective Method ◽  
H Pylori ◽  
Helicobacter Pylori Gastritis

Abstract Objectives.—To describe the endoscopic biopsy pathology of Helicobacter pylori gastritis, compare bacterial detection by immunohistochemistry using a specific antibody with the Genta stain, and to compare the relative costs of the 2 techniques. Design.—One hundred cases of gastritis identified as positive for H pylori by Genta stain and 100 cases considered negative by the same technique were stained using an anti-H pylori–specific polyclonal antibody. Laboratory reagent and labor costs for the 2 methods were compared. Results.—Chronic active gastritis with lymphoid follicles was significantly associated with H pylori infection (P &lt; .0001). The immunohistochemical method had a sensitivity of 97% and a specificity of 98% compared with the Genta stain, with strong agreement for grading density of organisms (κ = 0.85; P &lt; .001). Reagent costs were similar for both methods, but immunohistochemistry using an autoimmunostainer required less dedicated technical time and hence was less expensive than the Genta stain. Conclusions.—Immunohistochemistry using a specific antibody is an accurate and cost-effective method for H pylori detection in gastric biopsies.

scholarly journals Evaluation of Helicobacter pylory colonization by serologic test (IgG) and dyspepsia in volunteers from the countryside of Monte Negro, in the Brazilian western Amazon region

2010 ◽  
Vol 52 (4) ◽  
pp. 203-206 ◽  
Author(s):  
Rafael Bernardon Ribeiro ◽  
Herlon Saraiva Martins ◽  
Vera Aparecida dos Santos ◽  
Marcelo el Khouri ◽  
Leandro Savoy Duarte ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Rural Areas ◽  
Age Groups ◽  
Developed Countries ◽  
Progressive Increase ◽  
Amazon Region ◽  
Statistical Association ◽  
Serologic Test ◽  
Positive Serology ◽  
H Pylori

The present study intended to analyze the seroprevalence of Helicobacter pylori, IgG, and its relation to dyspepsia in a population from the western Amazon region. During the "Projeto Bandeira Científica", a University of São Paulo Medical School program, in Monte Negro's rural areas, state of Rondônia, 266 blood samples were collected from volunteers. The material was tested for IgG antibodies anti-Helicobacter pylori by ELISA method and the participants were also interviewed on dyspepsia, hygiene and social aspects. Participants aged between five and 81 years old (34 years on average), 149 (56%) were female and 117 (44%) male. We found 210 (78.9%) positive, 50 (18.8%) negative and six (2.3%) undetermined samples. Dyspeptic complaints were found in 226 cases (85.2%). There was no statistical association between dyspepsia and positive serology for H. pylori. We concluded that the seroprevalence in all age categories is similar to results found in other studies conducted in developing countries, including those from Brazil. On the other hand, the seroprevalence found in Monte Negro was higher than that reported in developed countries. As expected, there was a progressive increase in the positivity for H. pylori in older age groups.

scholarly journals Antecedents of E-Banking Services by Customers for the Selected Commercial Banks in Sylhet, Bangladesh

2016 ◽  
Vol 9 (1) ◽  
pp. 47
Author(s):  
Mohammad Zahed Hossain
Keyword(s):  
Mobile Apps ◽  
Age Groups ◽  
Cost Effective ◽  
Internet Banking ◽  
Demographic Variables ◽  
Online Banking ◽  
Primary Data ◽  
Mobile Banking ◽  
Chi Square ◽  
Banking Services

<p>This study is conducted to identify customers view regarding cost effectiveness, time savings and security of different types of e-banking products like online banking, ATM banking, internet banking, mobile banking and telephone banking. E-banking is the alternative delivery channels that banks adopted for providing efficient banking services through the help of internet, computers, mobile phone etc. Banks’ customers were considered as population and primary data were collected through questionnaire. Descriptive statistics and Chi-square test were used for analyzing the data. The results indicated that customers prefer ATM banking services most, next to follow mobile banking and online banking. The customers believed that all types of e-banking products save time and except telephone banking others types of e-banking products were secured. Online banking and ATM banking services were not considered as cost effective. Analysis indicated no relationship between online banking and different demographic variables. ATM banking services was highly influenced by most of the demographic variables whereas internet banking, mobile banking and telephone banking influenced by few demographic variables i.e. age groups, education level, and monthly income. The results help banks to develop varieties of e-banking products and formulate strategies by considering the demographic characteristics of the customers. Customers expect more users friendly e-banking products along with diversify features and suggested to develop latest e-banking products like mobile apps based banking for ensuring long term customers relationship, attracting potential customers and keeping existing customers that may ensure consistent growth and profit as well.</p>

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