Airborne Alt a 1 Dynamic and Its Relationship With the Airborne Dynamics of Alternaria Conidia and Pleosporales Spores

2021 ◽  
Author(s):  
Concepción De Linares ◽  
David Navarro ◽  
Rut Puigdemunt ◽  
Jordina Belmonte
Keyword(s):  
Public Health Service ◽  
Fungal Spores ◽  
High Volume ◽  
Major Allergen ◽  
Allergic Reactions ◽  
Aerobiological Monitoring ◽  
Treatment And Prevention ◽  
First Time ◽  
Prevention Of Allergy ◽  
Detection And Quantification

Fungal spores are universal atmospheric components associated to allergic reactions. Alternaria (Ascomycota) is considered the most allergenic spore taxa. Alt a 1 is the major allergen of Alternaria and is present also in other Pleosporales. In this study, standard Hirst-based sampling and analyzing methods for measuring spore daily concentrations of Alternaria, Curvularia, Drechslera-Helminthosporium, Epicoccum, Leptosphaeria, Pithomyces, Pleospora and Stemphyllium (all included in the taxon Pleosporales) have been used besides two high-volume samplers, Burkard Cyclone (2017) and MCV CAV-A/mb (2019-2020), and ELISA Kits for measuring the allergen. The detection and quantification of Alt a 1 was only possible in the samples from the MCV sampler. Although Alt a 1 was better correlated with Alternaria spores than with Pleosporales spores, the three of them showed high correlations. It is shown, for the first time, a high and significant correlation of Alt a 1 with temperature, a negative one with relative humidity and no correlation with precipitation. The aerobiological monitoring of these three elements ensures the best information for understanding the affectation to allergy sufferers but, if not possible, as a minimum public health service aiming at the detection, treatment and prevention of allergy, the study of the airborne Alternaria spores should be ensured.

Detection and Quantification of 7-Hydroxydehydroepiandrosterone Epimers in Three Body Fluids

2002 ◽  
Vol 67 (1) ◽  
pp. 10-18 ◽  
Author(s):  
Richard Hampl ◽  
Martin Hill ◽  
Luboslav Stárka
Keyword(s):  
Healthy Subjects ◽  
Serum Levels ◽  
Body Fluids ◽  
Gas Chromatography Mass Spectrometry ◽  
Specific Radioimmunoassay ◽  
Order Of Magnitude ◽  
Three Body ◽  
First Time ◽  
Nanomolar Range ◽  
Detection And Quantification

3β,7α-Dihydroxyandrost-5-en-17-one (1) (7α-OH-DHEA) and its 7β-hydroxy epimer 2 (7β-OH-DHEA) - 7α- and 7β-hydroxydehydroepiandrosterone - were detected and quantified in three human body fluids: in blood serum, saliva and ejaculate. Specific radioimmunoassay and gas chromatography-mass spectrometry have been used. For the first time the data on changes of these dehydroepiandrosterone metabolites are reported for a representative group of healthy subjects of both sexes (172 females and 217 males) during the life span. The serum levels of both 7-hydroxydehydroepiandrosterone epimers in serum and also in semen were in the low nanomolar range, while concentrations by one order of magnitude lower were found in saliva, but still within the detection limit. The results will serve as a basis for comparative studies of 7-hydroxydehydroepiandrosterone levels under various pathophysiological conditions, with a particular respect to autoimmune disorders.

Aerobiological monitoring for fungal spores in a rehabilitation hospital in Northern Italy

Aerobiologia ◽  
1996 ◽  
Vol 12 (1) ◽  
pp. 233-237
Author(s):  
Antonio Meriggi ◽  
Stefano Ricci ◽  
Mariella Bruni ◽  
Renato Corsico
Keyword(s):  
Fungal Spores ◽  
Northern Italy ◽  
Aerobiological Monitoring ◽  
Rehabilitation Hospital

scholarly journals Evaluating the Safety and Efficacy of a Highly Viscous 33-mg/mL Hyaluronic Acid Volumizing Filler in the Treatment of Facial Wrinkles: An Open-Labeled, Clinical Trials

2019 ◽  
Vol 8 ◽  
pp. e1148
Author(s):  
Elnaz Razavian ◽  
Setareh Tehrani
Keyword(s):  
Hyaluronic Acid ◽  
High Volume ◽  
Soft Tissue Augmentation ◽  
Nodule Formation ◽  
Safety And Efficacy ◽  
Significant Correction ◽  
Clinically Significant ◽  
One Year ◽  
First Time

Background: The 33-mg/mL hyaluronic acid (HA) formulation is a highly concentrated, cross-linked, cohesive, smooth, and completely reversible volumizing filler approved by Conformité Européene. For the first time, we aimed to evaluate the long-term efficacy and safety of the 33-mg/mL HA filler for soft tissue augmentation in the treatment of facial wrinkles. Materials and Methods: After optimal wrinkle correction was achieved in the patients undergoing treatment by injecting the 33-mg/mL HA filler at the injection site plus one touch-up at a 2-week interval, the safety and efficacy of the filler were assessed on the 5-point Facial Volume Loss Scale through the 1-year study period. Patients were evaluated daily for 14 days and after 6 and 12 months post-treatment. Results: A total of 86 subjects were treated. The mean wrinkle scores of the patients were 3.95+0.79 (range of 3-5) before treatment, 2.3+0.94 (range 1-5) six months after treatment, and 2.93+1.29 (range of 1-5) one year after treatment. Clinically significant mean wrinkle correction (P=0.001) was still evident at>12 months of treatment through 33-mg/mL HA formulation. A clinically significant correction at>12 months after treatment was maintained by 79% of patients. Nodule formation and swelling were more frequent when the 33-mg/mL HA filler was used compared with the use of less concentrated HA fillers. One patient developed angioedema-like swelling and induration last few months. Conclusion: The 33-mg/mL HA filler can provide long-term correction lasting for one year or more. Adverse effects, especially swelling and nodule formation were more common in this filler compared with less concentrated HA fillers. The side effects were correlated with the volume of the injected filler. We recommend using this concentration with low volume or combining high volume with lower concentration. [GMJ.2019;8:e1148]

scholarly journals Development of a Sandwich Enzyme-Linked Immunosorbent Assay for Detection and Quantification of Clam Residues in Food Products

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Stef J. Koppelman ◽  
Ashley L. Lardizabal ◽  
Lynn Niemann ◽  
Joe L. Baumert ◽  
Steve L. Taylor
Keyword(s):  
Sandwich Elisa ◽  
Food Product ◽  
Food Products ◽  
Elisa Test ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Allergic Reactions ◽  
Arctica Islandica ◽  
Limit Of Quantification ◽  
Detection And Quantification

Seafood is a frequent cause of allergic reactions to food globally. The presence of undeclared trace amounts of clam can cause allergic reactions in sensitive individuals. Limited tools are available to test food products for the presence of traces of clam. We report on the development of a sandwich ELISA that can detect and quantify clam protein in food. Antisera against a mix of two commercially important clam species, Atlantic Surf (Spisula solidissima) and ocean quahog (Arctica islandica), were raised in rabbit and sheep. A sandwich ELISA was constructed with this antisera, and sensitivity and specificity were evaluated. Also, model food products spiked with clam protein were analyzed to assess the performance of the ELISA. Comparison was made with a commercially available ELISA for crustacea. The lower limit of quantification of the sandwich ELISA is 2.5 ppm clam protein in food samples, allowing the detection of low amounts of clam that may trigger a reaction in clam allergic patients. The sandwich ELISA was highly specific with cross-reactivity only noted for other molluscan shellfish (mussel and scallop). Clam protein in tomato juice and potato cream soup was detected well with recoveries ranging from 65 to 74% and from 74 to 113%, respectively. However when potato cream soup was retorted, the recover fell to 20%, imposing the risk of underestimating the clam content of a food product. A commercially available crustacean ELISA test was not suitable to detect clam protein. The sandwich ELISA described here is suitable for detection and quantification of clam protein in food products. Care should be taken with food products that have been retorted as the results may be underestimated.

scholarly journals Allergenic fungal spores in the air of urban parks

Aerobiologia ◽  
2020 ◽  
Author(s):  
Idalia Kasprzyk ◽  
Agnieszka Grinn-Gofroń ◽  
Agata Ćwik ◽  
Katarzyna Kluska ◽  
Paloma Cariñanos ◽  
...  
Keyword(s):  
Fungal Spores ◽  
Urban Parks ◽  
Volumetric Method ◽  
Green Spaces ◽  
Seasonal Occurrence ◽  
Aerobiological Monitoring ◽  
Urban Green ◽  
Urban Green Spaces ◽  
Airborne Fungal Spores ◽  
Burkard Sampler

Abstract Urban green spaces, especially urban parks, are essential for the proper functioning of cities, but they can be a serious source of airborne fungal spores. Aerobiological monitoring was carried out in urban parks of different typology to estimate the risk associated with fungal spores for citizens. Volumetric method was applied with the use of portable Burkard Sampler. In the air of the studied parks, the most dominant spores are strong allergenic or considered as potentially allergenic. Cladosporium spores were found in enormous concentrations in all studied parks, and it affected the low biodiversity of fungal spores in the parks. Compared to Cladosporium, concentrations of Alternaria spores in the air were several dozen times lower, but still a risk for people who are allergic. The fungal spores spectra and their seasonal occurrence in each park were similar. The highest similarities in the patterns of the season were found in the case of Cladosporium, Alternaria, Epicoccum, and the lowest in the case of Torula and Drechslera type. Due to the fact that allergy sufferers are most often polysensitized, the period when they should limit long visits in the urban parks is July–August, when the concentration of allergenic fungal spores of many taxa is the highest.

Oestrogenic action of neonatal tamoxifen on the hypothalamus and reproductive system in female mice

2017 ◽  
Vol 29 (5) ◽  
pp. 1012 ◽  
Author(s):  
Rahmatollah Parandin ◽  
Morteza Behnam-Rassouli ◽  
Nasser Mahdavi-Shahri
Keyword(s):  
Reproductive System ◽  
Reproductive Function ◽  
Female Mice ◽  
Treatment And Prevention ◽  
Receptor Modulator ◽  
Neuron Populations ◽  
Reproductive Axis ◽  
First Time ◽  
The Brain ◽  
Selective Oestrogen Receptor Modulator

Tamoxifen, a selective oestrogen receptor modulator, is widely used for both the treatment and prevention of breast cancer in women; however, it is known to have adverse effects in the female reproductive system. Growing evidence suggests that oestrogen-sensitive neuron populations of the anteroventral periventricular (AVPV) nucleus and arcuate (ARC) nucleus, especially kisspeptin neurons, play a pivotal role in the timing of puberty onset and reproductive function. The aim of the present study was to evaluate whether neonatal exposure to tamoxifen affects oestrogenic actions in the brain and reproductive function in mice. On 1 to 5 postnatal days, female pups were injected subcutaneously with sesame oil (sham), oestradiol benzoate (EB; 20 µg kg–1), tamoxifen (0.4 mg kg–1) or EB+tamoxifen. Control mice received no treatment. Mice in the EB, tamoxifen and tamoxifen+EB groups exhibited advanced vaginal opening, disrupted oestrous cycles and a decreased follicular pool. Conversely, in these groups, there was a reduction in kisspeptin (Kiss1) mRNA expression, the neuronal density of AVPV and ARC nuclei and LH and oestradiol concentrations in the serum. The results of the present study confirm oestrogenic actions of tamoxifen in the brain and reproductive system. In addition, we show, for the first time, that tamoxifen has oestrogenic effects on the oestrogen-sensitive hypothalamic AVPV and ARC nuclei controlling the reproductive axis in female mice.

Deposition of nanoplastics in high-altitude Alpine snow

2020 ◽  
Author(s):  
Dusan Materic ◽  
Elke Ludewig ◽  
Simona M. Cristescu ◽  
Thomas Röckmann ◽  
Rupert Holzinger
Keyword(s):  
High Altitude ◽  
Environmental Samples ◽  
Common Type ◽  
New Method ◽  
Methodological Issues ◽  
Remote Areas ◽  
Surface Snow ◽  
Daily Record ◽  
First Time ◽  
Detection And Quantification

<p>Recent research discovered that airborne microplastics pollution can reach very remote areas. However, analysis of nanoplastics in environmental samples remains challenging, mostly due to technical and methodological issues. A new method for chemical characterisation of nanoplastics based on TD-PTR-MS has been recently introduced. The detection limit of <1ng allowed for the first time the analysis of nanoplastics deposited on the snow in the pristine Alps. In this work, we analysed daily samples of surface snow close to the Sonnblick Observatory, Austria (3106 m altitude) in the period from 2017-02-07 to 2017-03-19, using our new method. The results showed a positive detection for various types of nanoplastics, and the most common type found was Polyethylene terephthalate (PET). We will present our results on the to-date longest daily record of nanoplastics deposition in high altitude regions and further discuss optimisations of TD-PTR-MS method for nanoplastics detection and quantification.</p>

scholarly journals Characterization of the Group I Allergen of Bahia Grass Pollen

2009 ◽  
Vol 2 (1) ◽  
pp. 27-29 ◽  
Author(s):  
J. M. White ◽  
A. Majidi ◽  
S. A. Naser ◽  
M. J. Sweeney ◽  
R. S. White
Keyword(s):  
Grass Pollen ◽  
Allergic Patient ◽  
Major Allergen ◽  
Terminal Amino Acid ◽  
Molecular Weights ◽  
Group I ◽  
Allergenic Proteins ◽  
Terminal Amino ◽  
First Time

Four allergenic proteins of Bahia (BA) grass pollen with estimated molecular weights of 45, 33, 31 and 28 kD were previously detected. Although all four proteins were reactive with BA grass allergic patient sera, the 33kD component was previously verified as a major allergen. The investigators report here for the first time, the similarities between the group I 33 kD allergen of BA grass, Pas n 1, and Group I allergen of Timothy grass, Phl p 1, using N-terminal amino acid sequencing and monoclonal antibodies, IG12 and BOT14, made to Timothy Phl p 1.

scholarly journals Treatment and prevention of Salomellosis in puppies using Lactobacillus acidophilus

2008 ◽  
Vol 32 (1) ◽  
pp. 214-222
Author(s):  
S. A.G. Al-Samarrae
Keyword(s):  
Lactobacillus Acidophilus ◽  
Biochemical Parameters ◽  
Bile Salts ◽  
Intestinal Epithelial Cells ◽  
Low Ph ◽  
Intestinal Epithelial ◽  
Standard Strain ◽  
Treatment And Prevention ◽  
First Time

This study was carried out to evaluate the ability of Lactobacillusacidophilus to prevent and treat Salmonella typhimurium in puppies. In vitroantibiotic sensitivity test of S. typhimurium was made and the result revealedthat Ciprofloxacin is the most effective.Isolation of L. acidophilus from theintestinal tract of the normal dogs and also revealed that all Lactobacillus strainswere biochemically identical to standard strain. A bacterial strain that had highability to inhibit the growth of S. typhimurium in vitro with high ability toadhere to intestinal epithelial cells and tolerate the low pH and bile salts waschosen for the experimental study.Twenty puppies were divided into four groups and treated in different ways.The clinical, haematological and biochemical parameters were obtained from allanimals at the period of two days before inoculation until the death of animals ofthe first group at the sixth day post infection. The results revealed that , puppiesexperimentally infected with S.typhimurium showed both septicemic andgastrointestinal forms of the disease accompanied with isolation ofS.typhimurium from the blood and stool throughout the experiment. Thestatistical analysis of the results of all parameters among all groups revealed ,forthe first time, that L.acidophilus plays an important role in the prevention ofS.typhimurium infection in puppies . It also has high therapeutic effect againstS.typhimurium, which was almost similar to that of Ciprofloxacin.

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