scholarly journals Concentrated nanofat: a modified fat extraction promotes hair growth in mice via the stem cells and extracellular matrix components interaction

2020 ◽  
Vol 8 (18) ◽  
pp. 1184-1184
Author(s):  
Zehua Li ◽  
Jinrong Zhang ◽  
Meng Li ◽  
Lingzhi Tang ◽  
Hongwei Liu
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Hair Growth ◽  
Fat Extraction ◽  
Extracellular Matrix Components ◽  
Matrix Components

scholarly journals A Strategy to Enhance Secretion of Extracellular Matrix Components by Stem Cells: Relevance to Tissue Engineering

2018 ◽  
Vol 24 (1-2) ◽  
pp. 145-156 ◽  
Author(s):  
Navaneethakrishnan Krishnamoorthy ◽  
Yuan‐Tsan Tseng ◽  
Poornima Gajendrarao ◽  
Padmini Sarathchandra ◽  
Ann McCormack ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Extracellular Matrix ◽  
Extracellular Matrix Components ◽  
Matrix Components

scholarly journals Presence of ROS in Inflammatory Environment of Peri-Implantitis Tissue: In Vitro and In Vivo Human Evidence

2019 ◽  
Vol 9 (1) ◽  
pp. 38
Author(s):  
Eitan Mijiritsky ◽  
Letizia Ferroni ◽  
Chiara Gardin ◽  
Oren Peleg ◽  
Alper Gultekin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Human Model ◽  
Inflammatory Cell Infiltrate ◽  
Mitochondrial Activity ◽  
Type I ◽  
Biological Organization ◽  
Extracellular Matrix Components ◽  
Matrix Components

Analyses of composition, distribution of cellular and extracellular matrix components, and molecular analysis of mitochondria related genes of bone loss in the presence of inflammatory environment in humans was the aim of the present project. As a human model we chose peri-implantitis. Morphological analyses were performed by means classical histological, immunohistochemical, and SEM (scanning electron miscroscopy) test. Gene expression analysis was performed to evaluate epithelium maturation, collagen fiber production, and genes related to mitochondrial activity. It was found that a well-defined keratinocyte epithelium was present on the top of all specimens; a distinct basal lamina was present, as well as desmosomes and autophagic processes related to the maturation of keratinocytes. Under this epithelium, a full inflammatory cell infiltrate was present for about 60% of the represented by plasma cells. Collagen type I fibers were present mainly in the form of fragmented cord tissue without cells. A different distribution of blood vessels was also present from the apical to the most coronal portion of the specimens. High levels of genes related to oxidative stress were present, as well as the activation of genes related to the loss of ability of osteogenic commitment of Mesenchymal stem cells into osteoblasts. Our study suggests that peri-implantitis lesions exhibit a well defined biological organization not only in terms of inflammatory cells but also on vessel and extracellular matrix components even if no difference in the epithelium is evident, and that the presence of reactive oxygen species (ROS) related to the inflammatory environment influences the correct commitment of Mesenchymal stem cells.

scholarly journals Can We Build Artificial Stem Cell Compartments?

2003 ◽  
Vol 2003 (3) ◽  
pp. 164-169 ◽  
Author(s):  
Carlos E. Semino
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Three Dimensional ◽  
Soluble Factors ◽  
Cell Compartments ◽  
Synthetic Materials ◽  
Extracellular Matrix Components ◽  
Main Factors ◽  
Matrix Components

Animals carry stem cells throughout their entire life, from embryogenesis to senescence. Their function during development and adulthood consists basically of forming and sustaining functional tissues while maintaining a small self-renewing population. They reside in a complex three-dimensional environment consisting of other nearby cells extracellular matrix components, endogenous or exogenous soluble factors, and physical, structural, or mechanical properties of the tissues they inhabit. Can we artificially recreate tissue development such that stem cells can both self-renew and be instructed to mature properly? The main factors required to regulate the maintenance and differentiation of some types of stem cells are known. In addition, new bioengineered synthetic materials that mimic extracellular matrix components can be used as initial scaffolding for building stem cell microenvironments.

scholarly journals Age related extracellular matrix and interstitial cell phenotype in pulmonary valves

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shaohua Wu ◽  
Vikas Kumar ◽  
Peng Xiao ◽  
Mitchell Kuss ◽  
Jung Yul Lim ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Extracellular Matrix ◽  
Heart Valve ◽  
Cell Phenotype ◽  
Primary Concern ◽  
Ross Operation ◽  
Age Related ◽  
Cardiovascular Morbidity And Mortality ◽  
Extracellular Matrix Components ◽  
Matrix Components

AbstractHeart valve disease is a common manifestation of cardiovascular disease and is a significant cause of cardiovascular morbidity and mortality worldwide. The pulmonary valve (PV) is of primary concern because of its involvement in common congenital heart defects, and the PV is usually the site for prosthetic replacement following a Ross operation. Although effects of age on valve matrix components and mechanical properties for aortic and mitral valves have been studied, very little is known about the age-related alterations that occur in the PV. In this study, we isolated PV leaflets from porcine hearts in different age groups (~ 4–6 months, denoted as young versus ~ 2 years, denoted as adult) and studied the effects of age on PV leaflet thickness, extracellular matrix components, and mechanical properties. We also conducted proteomics and RNA sequencing to investigate the global changes of PV leaflets and passage zero PV interstitial cells in their protein and gene levels. We found that the size, thickness, elastic modulus, and ultimate stress in both the radial and circumferential directions and the collagen of PV leaflets increased from young to adult age, while the ultimate strain and amount of glycosaminoglycans decreased when age increased. Young and adult PV had both similar and distinct protein and gene expression patterns that are related to their inherent physiological properties. These findings are important for us to better understand the physiological microenvironments of PV leaflet and valve cells for correctively engineering age-specific heart valve tissues.

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