AB041. Establishment of age specific reference intervals of neonatal thyroid stimulating hormone in Pakistani population: experience at a tertiary care center

ABSTRACT Aim Maternal thyroid hormone levels during pregnancy are vital for the health of the mother as well as the developing child. Fetal growth is affected by maternal thyroid levels. Various physiological changes like alterations of thyroxine-binding globulins, human chorionic gonadotropin level, and changes in iodide metabolism affect maternal thyroid hormone levels. Therefore, reference intervals (RIs) for thyroid hormones in pregnant population require to be established separately from the general population. Materials and methods The RIs of serum triiodothyronine (T3), thyroxine (T4), and thyroid-stimulating hormone (TSH) were determined in healthy pregnant women by enzyme-linked immunosorbent assay (ELISA) technique after segregating them into three trimesters. This study was conducted in a 492-bedded zonal-level hospital. The reference population was chosen from a study population of pregnant women by strict inclusion and exclusion criteria. The assays were done by the most-commonly used, economical ELISA method employing standard kits. Tests were done using accurate and precise methods with proper quality control measures. Results The RIs were calculated from the central 95% of distribution of total T3, total T4, and TSH values located between 2.5 and 97.5 percentile values. The 0.90 confidence intervals for the upper and lower reference limits were calculated. The values thus obtained were different from those provided by the manufacturer kit literature. Conclusion It is recommended to determine one's own laboratory-specific, method-specific, trimester-wise RIs for maternal thyroid hormone status and use them for screening of pregnant women. How to cite this article Chakrabarty BK, Mitra B, Pal R, Hazra N. Specific Reference Intervals of Serum Triiodothyronine, Thyroxine, and Thyroid-stimulating Hormone in Normal Pregnant Indian Women as per Trimester. Indian J Med Biochem 2017;21(1):17-21.

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Study of cytodiagnosis of discharge per vaginum with specific reference to human papilloma virus and herpes simplex virus at tertiary care center, Indore

International Journal of Medical Science and Public Health ◽

10.5455/ijmsph.2017.0306524032017 ◽

2017 ◽

pp. 1

Author(s):

Khushboo Likhar ◽

Meena Mittal ◽

C Kulkarni ◽

Monica Verma

Keyword(s):

Herpes Simplex Virus ◽

Herpes Simplex ◽

Human Papilloma Virus ◽

Care Center ◽

Tertiary Care ◽

Specific Reference ◽

Tertiary Care Center ◽

Papilloma Virus ◽

Simplex Virus

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Establishment of trimester-specific thyroid stimulating hormone and free thyroxine reference interval in pregnant Chinese women using the Beckman Coulter UniCel™ DxI 600

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2014-0615 ◽

2015 ◽

Vol 53 (9) ◽

Cited By ~ 8

Author(s):

Jing Zhang ◽

Wei Li ◽

Qiao-Bin Chen ◽

Li-Yi Liu ◽

Wei Zhang ◽

...

Keyword(s):

Chinese Women ◽

Reference Interval ◽

Reference Intervals ◽

Thyroid Stimulating Hormone ◽

Free Thyroxine ◽

Specific Reference ◽

Thyroid Peroxidase ◽

Cross Sectional ◽

Reported Data ◽

Stimulating Hormone

AbstractThyroid-stimulating hormone (TSH) and free thyroxine (FT4) reference intervals are essential for screening and diagnosing thyroid dysfunction during pregnancy. The aim of this study was to establish method- and trimester-specific TSH and FT4 reference intervals in pregnant Chinese women using the Beckman Coulter UniCel™ DxI 600.A cross-sectional dataset analysis was performed. A total of 3507 participants were recruited, and 2743 were eligible for analysis to set reference intervals. TSH, FT4, and thyroid peroxidase antibody (TPOAb) levels were analyzed with the Beckman Coulter UniCel™ DxI 600 AccessThe calculated reference intervals for the first, second, and third trimesters were TSH: 0.06–3.13, 0.07–4.13 and 0.15–5.02 mIU/L, respectively, and FT4: 8.72–15.22, 7.10–13.55 and 6.16–12.03 pmol/L, respectively.Our reference intervals for TSH and FT4 are distinct from the ranges reported in the DxI 600 instruction manual and previously reported data, confirming the importance of method-specific reference intervals.

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Specific Reference Intervals of Serum Triiodothyronine, Thyroxine, and Thyroid-stimulating Hormone in Normal Pregnant Indian Women as per Trimester

Indian Journal of Medical Biochemistry ◽

10.5005/jp-journals-10054-0029 ◽

2017 ◽

Vol 21 (2) ◽

pp. 96-100

Author(s):

Nandita Hazra ◽

Binay Mitra ◽

Reetika Pal

Keyword(s):

Thyroid Hormone ◽

Pregnant Women ◽

Reference Intervals ◽

Thyroid Stimulating Hormone ◽

Enzyme Linked Immunosorbent Assay ◽

Specific Reference ◽

Specific Method ◽

Indian Women ◽

Maternal Thyroid ◽

Stimulating Hormone

ABSTRACT Aim Maternal thyroid hormone level during pregnancy is a vital parameter for the health of mother as well as developing child. Fetal growth is affected by maternal thyroid levels. Various physiological changes like alterations of thyroxine binding globulins (TBGs), beta-human chorionic gonadotropin (β-hCG) level, and change of iodide metabolism affect maternal thyroid hormone levels. Therefore, reference intervals (RI) for thyroid hormones in pregnant population require to be established separately from general population. Materials and methods The RIs of serum triiodothyronine (T3), thyroxine (T4), and thyroid-stimulating hormone (TSH) were determined in healthy pregnant women by enzyme-linked immunosorbent assay (ELISA) technique after segregating them into three trimesters. This study was conducted in a 492-bedded zonal level hospital. The reference population was chosen from a study population of pregnant women by strict inclusion and exclusion criteria. The assays were done by the most commonly used economical ELISA method using standard kits. Tests were done using accurate and precise methods with proper quality control measures. Results The RIs were calculated from the central 95% of distribution of total T3, total T4, and TSH values located between 2.5 and 97.5 percentile values. The 0.90 confidence intervals (CIs) for the upper and lower reference limits were calculated. The values thus obtained were different from those provided by manufacturer kit literature. Conclusion It is recommended to determine own laboratory-specific, method-specific, trimester-wise RI for maternal thyroid hormone status and use them for screening of pregnant mothers. How to cite this article Chakrabarty BK, Mitra B, Pal R, Hazra N. Specific Reference Intervals of Serum Triiodothyronine, Thyroxine, and Thyroid-stimulating Hormone in Normal Pregnant Indian Women as per Trimester. Indian J Med Biochem 2017;21(2):96-100.

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Preoperative thyroid-stimulating hormone associated risk of differentiated thyroid cancer in patients with thyroid nodules

Cellular and Molecular Biology ◽

10.14715/cmb/2021.67.3.11 ◽

2021 ◽

Vol 67 (3) ◽

pp. 85-91

Author(s):

Al Essa M

Keyword(s):

Thyroid Cancer ◽

Differentiated Thyroid Cancer ◽

Thyroid Nodules ◽

Conditional Logistic Regression ◽

Care Center ◽

Tertiary Care ◽

Thyroid Stimulating Hormone ◽

Normal Range ◽

Tsh Levels ◽

Stimulating Hormone

In an assessment of risk for differentiated thyroid cancer (DTC) in individuals with human papillary thyroid cancer (PTC) and thyroid nodules a cohort prospective study was undertaken to establish the significance of preoperative thyroid-stimulating hormone (TSH) levels. Confirmed histologically PTC cases in one tertiary care center, and matched healthy individuals were tested for TSH, T3, T4 and T4 free total. The ORs and 95% confidence intervals have been calculated using conditional logistic regression models (CI). The blood TSH levels were related to the higher risk of PTC for men (OR,0,09; 95% Ci, 04–0,21, 95% CI and women) compared with the middle tertile of the TSH levels in the normal range (OR,0,07; 95 percent CI, 0,04–0,1). Over the normal range of TSH levels, an elevated PTC risks were connected amongst women (OR 0,09; 95% CI, 0,04–0,21) but not amongst men (OR,0,07; 95% CI, 0,04–0,1). With an increase in TSH level in the normal range between men and women, the risk for PTC reduced (Ptrend=0.041 and 0.0001). The risk of PTC related to TSH levels has been dramatically elevated above the normal range for men and TSH values below the normal range for women.

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Indirect determination of hematology reference intervals in adult patients on Beckman Coulter UniCell DxH 800 and Abbott CELL-DYN Sapphire devices

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2018-0771 ◽

2019 ◽

Vol 57 (5) ◽

pp. 730-739 ◽

Cited By ~ 5

Author(s):

Jakob Zierk ◽

Farhad Arzideh ◽

Rainer Haeckel ◽

Manfred Rauh ◽

Markus Metzler ◽

...

Keyword(s):

Clinical Laboratory ◽

Care Center ◽

Tertiary Care ◽

Sample Selection ◽

Reference Intervals ◽

Red Cell ◽

Cell Distribution ◽

Tertiary Care Center ◽

Test Results ◽

Distribution Width

Abstract Background Conventional establishment of reference intervals for hematological analytes is challenging due to the need to recruit healthy persons. Indirect methods address this by deriving reference intervals from clinical laboratory databases which contain large datasets of both physiological and pathological test results. Methods We used the “Reference Limit Estimator” (RLE) to establish reference intervals for common hematology analytes in adults aged 18–60 years. One hundred and ninety-five samples from 44,519 patients, measured on two different devices in a tertiary care center were analyzed. We examined the influence of patient cohorts with an increasing proportion of abnormal test results, compared sample selection strategies, explored inter-device differences, and analyzed the stability of reference intervals in simulated datasets with varying overlap of pathological and physiological test results. Results Reference intervals for hemoglobin, hematocrit, red cell count and platelet count remained stable, even if large numbers of pathological samples were included. Reference intervals for red cell indices, red cell distribution width and leukocyte count were sufficiently stable, if patient cohorts with the highest fraction of pathological samples were excluded. In simulated datasets, estimated reference limits shifted, if the pathological dataset contributed more than 15%–20% of total samples and approximated the physiological distribution. Advanced sample selection techniques did not improve the algorithm’s performance. Inter-device differences were small except for red cell distribution width. Conclusions The RLE is well-suited to create reference intervals from clinical laboratory databases even in the challenging setting of a adult tertiary care center. The procedure can be used as a complement for reference interval determination where conventional approaches are limited.

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Pediatric reference intervals for endocrine markers and fertility hormones in healthy children and adolescents on the Siemens Healthineers Atellica immunoassay system

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2021-0050 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Mary Kathryn Bohn ◽

Paul Horn ◽

Donna League ◽

Paul Steele ◽

Alexandra Hall ◽

...

Keyword(s):

Clinical Decision Making ◽

Rapid Development ◽

Reference Intervals ◽

Thyroid Stimulating Hormone ◽

Specific Reference ◽

Childhood And Adolescence ◽

Healthy Children ◽

Serum Samples ◽

Age And Sex ◽

Stimulating Hormone

Abstract Objectives Rapid development in childhood and adolescence combined with lack of immunoassay standardization necessitates the establishment of age-, sex-, and assay-specific reference intervals for immunochemical markers. This study established reference intervals for 11 immunoassays on the new Siemens Healthineers Atellica® IM Analyzer in the healthy CALIPER cohort. Methods A total of 600 healthy participants (birth to 18 years) were recruited from the community, and serum samples were collected with informed consent. After sample analysis, age- and sex-specific differences were assessed, and outliers were removed. Reference intervals were established using the robust method (40–<120 participants) or nonparametric method (≥120 participants). Results Of the 11 immunoassays studied, nine required age partitioning (i.e., dehydroepiandrosterone-sulfate, estradiol, ferritin, folate, follicle-stimulating hormone, luteinizing hormone, progesterone, testosterone, vitamin B12), and seven required sex partitioning. Free thyroxine and thyroid-stimulating hormone demonstrated no significant age- and/or sex-specific differences. Conclusions Overall, the age- and sex-specific trends observed closely mirrored those previously reported by CALIPER on other platforms as well as other internationally recognized studies. However, established lower and upper limits demonstrated some discrepancies between published values from healthy cohorts on alternate analytical systems, highlighting differences between manufacturers and the need for platform-specific reference intervals for informed pediatric clinical decision-making.

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