scholarly journals GENETIC VARIABILITIES OF AGRONOMIC TRAITS AND BACTERIAL LEAF BLIGHT RESISTANCE OF HIGH YIELDING RICE VARIETIES

2020 ◽  
Vol 20 (2) ◽  
pp. 43
Author(s):  
Nafisah Nafisah ◽  
Celvia Roza ◽  
Nani Yunani ◽  
Aris Hairmansis ◽  
Tita Rostiati ◽  
...  
Keyword(s):  
Agronomic Traits ◽  
Genetic Resistance ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Yield Potential ◽  
Blight Resistance ◽  
Wet Season ◽  
Rice Varieties ◽  
Superior Genotypes ◽  
Productive Tiller

<p class="abstrakinggris">Hundred of high yielding and bacterial leaf blight (<em>Xanthomonas oryzae</em> pv. <em>oryzae, Xoo</em>) resistant rice varieties released since the 1960s are important sources of genetic materials for exploring superior genotypes. The study aimed to evaluate the genetic resistance of 177 rice varieties to <em>Xoo</em> and their agronomic traits. The evaluations were conducted at the Indonesian Center for Rice Research Experimental Station during the wet season (December 2015-March 2016). The bacterial leaf blight resistance was evaluated for <em>Xoo</em> pathotypes III, IV, and VIII using the clipping method. The genetic variation among genotypes was categorized as low (0–10%), medium (10–20%), and high (&gt;20%), whereas the heritability was categorized as low (0-30%), medium (30-60%), and high (&gt;60%). The variability of resistance to <em>Xoo</em> pathotypes, grain yield, and spikelet fertility was low, while the variability of plant height, productive tiller number, filled grain, and total spikelet was medium, and the variability of unfilled grain number was high. The 29 varieties  were categorized as superior based on their agronomic traits or resistance to <em>Xoo</em> pathotypes. In conclusion, Batutegi and Fatmawati were superior in the total spikelet number, while Rojolele and Inpari 2 were supreme in the thousand-grain weight. Dodokan had a very short maturity, and Inpari 24, Conde, Kalimas, Angke, Inpari 17, and Inpara 8 had the highest resistance to <em>Xoo</em> pathotypes. The study implies that the identified rice superior genotypes could be used as genetic materials to design cross combinations for higher yield potential and BLB resistance varietal improvement.</p>

scholarly journals PEMANFAATAN PIRAMIDA GEN KETAHANAN TERHADAP PENYAKIT HAWAR DAUN BAKTERI DALAM MENDUKUNG PERAKITAN VARIETAS UNGGUL PADI Application of Gene Pyramiding For Resistance to Bacterial Leaf Blight to Develop New Rice Variety

2020 ◽  
Vol 39 (1) ◽  
pp. 11
Author(s):  
Fatimah Fatimah ◽  
Joko Prasetiyono
Keyword(s):  
Molecular Marker ◽  
Rice Variety ◽  
Gene Pyramiding ◽  
Leaf Blight ◽  
Rice Production ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Yield Potential ◽  
Rice Varieties ◽  
Marker Assisted Backcrossing

<p>Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv oryzae (Xoo) is an important bacterial disease and very destructive to rice plant. BLB decreased rice production from 20%-30% up to 80%. Host-plant resistance is a cost-effective and environmentally safe approach to reduce yield loss. However the development of new rice variety by conventional selection would take several years. The genetic improvement in rice production considered as a vital program in order to ensure national food security. The availability of corresponding molecular marker makes it more precision and efficient by reducing the time required for selection. This present article highlights the molecular approach in breeding for BLB disease resistant rice varieties. In detail, it will be discussed the application of molecular marker assisted backcrossing and pyramiding gene resistance offered breeders to accelerate the rice breeding program for resistance to BLB. The pyramiding of three resistance BLB genes (xa5, Xa7, and Xa21and one gene (Xa4) as a background into two elite indica rice varieties, Ciherang and Inpari 13, was introduced successfully. The combining of conventional breeding, marker assisted backcrossing, disease evaluation, agronomic performance and yield has led the significant resistance of pyramid lines to Xoo Race III, IV and VIII in vegetative and generative phase while their yield potential was maintained (6-7 ton/ha). The current status of Ciherang-HDB and Inpari 13-HDB pyramid lines is the production of nucleoseeds and breeder seeds. This broad spectrum and durable resistance characteristic may help in controlling BLB disease in different region of Indonesia and it will facilitate the rice self-sustainability program.</p><p>Keywords: Rice, gene pyramiding, plant breeding, molecular marker.</p><p> </p><p><strong>Abstrak</strong></p><p>Penyakit hawar daun bakteri (HDB) yang disebabkan oleh Xanthomonas oryzae pv oryzae (Xoo) merupakan penyakit penting pada tanaman padi karena dapat menurunkan produksi padi rata-rata 20-30% bahkan dapat mencapai 80%. Penggunaan varietas tahan merupakan cara pengendalian yang paling efektif, ramah lingkungan, dan mudah dilakukan. Namun pengembangan varietas unggul baru melalui seleksi konvensional memerlukan waktu lebih lama. Perbaikan varietas padi perlu terus dikembangkan dalam mendukung ketahanan pangan dan kemandirian pangan nasional. Tersedianya marka molekuler membantu proses pemuliaan tanaman menjadi lebih presisi dan lebih efisien sehingga mengurangi waktu seleksi pada tanaman progeni. Tulisan ini memfokuskan pendekatan molekuler dalam pemuliaan varietas tahan penyakit HDB melalui piramida gen ketahanan untuk mempercepat progam pemuliaan padi tahan penyakit HDB. Kegiatan menggabungkan tiga gen ketahanan (xa5, Xa7, dan Xa21) dan satu gen (Xa4) sebagai background ke dalam padi varietas Ciherang dan Inpari-13 telah berhasil dilakukan. Melalui penggabungan beberapa pendekatan yaitu pemuliaan konvensional dan silang balik berbantu marka, evaluasi penyakit dan keragaan agronomi serta komponen hasil telah menunjukkan peningkatan ketahanan yang nyata pada galur-galur piramida Ciherang HDB dan Inpari-13 HDB pada tiga ras Xoo (Ras III, IV, dan VIII), baik pada fase vegetatif maupun generatif dengan potensi hasil tidak berbeda nyata dengan tetuanya (6-7 t/ha). Saat ini sudah diproduksi benih inti (NS) dan benih penjenis (BS) galur-galur piramida Ciherang HDB dan Inpari-13 HDB. Dengan demikian, galur-galur piramida memiliki spektrum yang luas dan mampu bertahan dalam jangka waktu lama sehingga dapat mengontrol penyakit HDB di berbagai wilayah Indonesia dan mendukung target pemerintah untuk mempertahankan swasembada beras secara berkelanjutan.</p><p>Kata kunci: Padi, piramida gen, pemuliaan tanaman, marka molekuler.<br /><br /></p><p> </p>

scholarly journals Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0255470
Author(s):  
Phuong Nguyen Duy ◽  
Dai Tran Lan ◽  
Hang Pham Thu ◽  
Huong Phung Thi Thu ◽  
Ha Nguyen Thanh ◽  
...  
Keyword(s):  
Agronomic Traits ◽  
Rice Variety ◽  
Rice Cultivar ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Transcription Activator ◽  
Rice Varieties ◽  
Sugar Transporters ◽  
Blight Disease ◽  
Mutant Lines

TBR225 is one of the most popular commercial rice varieties in Northern Vietnam. However, this variety is highly susceptible to bacterial leaf blight (BLB), a disease caused by Xanthomonas oryzae pv. oryzae (Xoo) which can lead to important yield losses. OsSWEET14 belongs to the SWEET gene family that encodes sugar transporters. Together with other Clade III members, it behaves as a susceptibility (S) gene whose induction by Asian Xoo Transcription-Activator-Like Effectors (TALEs) is absolutely necessary for disease. In this study, we sought to introduce BLB resistance in the TBR225 elite variety. First, two Vietnamese Xoo strains were shown to up-regulate OsSWEET14 upon TBR225 infection. To investigate if this induction is connected with disease susceptibility, nine TBR225 mutant lines with mutations in the AvrXa7, PthXo3 or TalF TALEs DNA target sequences of the OsSWEET14 promoter were obtained using the CRISPR/Cas9 editing system. Genotyping analysis of T0 and T1 individuals showed that mutations were stably inherited. None of the examined agronomic traits of three transgene-free T2 edited lines were significantly different from those of wild-type TBR225. Importantly, one of these T2 lines, harboring the largest homozygous 6-bp deletion, displayed decreased OsSWEET14 expression as well as a significantly reduced susceptibility to a Vietnamese Xoo strains and complete resistance to another one. Our findings indicate that CRISPR/Cas9 editing conferred an improved BLB resistance to a Vietnamese commercial elite rice variety.

scholarly journals Phenotype and genotype variability of interspecific rice lines related to bacterial leaf blight resistance (Xanthomonas oryzae pv. oryzae) character

2021 ◽  
Vol 22 (10) ◽  
Author(s):  
SITI YURIYAH ◽  
DEDY DARNAEDI ◽  
TATANG MITRA SETIA ◽  
GUT WINDARSIH ◽  
Dwinita Wikan Utami
Keyword(s):  
Resistance Gene ◽  
Wild Rice ◽  
Leaf Blight ◽  
Oryza Rufipogon ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Yield Potential ◽  
Interspecific Crosses ◽  
Blight Resistance ◽  
Wild Rice Species

Abstract. Yuriyah S, Darnaedi D, Setia TM, Windarsih G, Utami DW. 2021. Phenotype and genotype variability of interspecific rice lines related to bacterial leaf blight resistance (Xanthomonas oryzae pv. oryzae) character. Biodiversitas 22: 4123-4130. Wild rice species are the source of the gene pool for rice genetic diversity. The cross-species crosses (interspecific crosses) play an important role in breeding, namely in terms of expanding the diversity of desirable characters, such as disease resistance and improvement in yield potential. Currently, the genes from wild rice species have been successfully introgressed into cultivated rice so that they can overcome the rice production constraints, including the introgression of genes for the bacterial leaf blight (BLB) resistance. The purpose of this study was to analyze the variability of phenotype and genotype performance of lines derived from wild rice species for the character of resistance to BLB disease. A total of 33 selected backcross lines derived from wild rice species of O. rufipogon and O. glaberrima as the donor parents and 2 control varieties (resistant and susceptible to BLB disease), were used in this study. The evaluation of phenotype and genotype of resistance characters to BLB disease was carried out during a vegetative phase of the plant. The resistance evaluation was carried out in a greenhouse, while the genotype performance was analyzed using 4 STS markers linked to Xa4, Xa7, and Xa13 genes. The result of the phenotype evaluation showed that there were variations among the resistance of the tested lines. Three lines derived from a Situ Bagendit/Oryza rufipogon cross were resistant to all BLB races used in the testing. Those three lines indicated to have the allele of the Xa7 resistance gene based on the genotype performance which grouped with the Code variety that had the Xa7 resistance gene.

scholarly journals Research Article Identification of bacterial leaf blight resistance genes in Malaysian local rice varieties

2020 ◽  
Vol 19 (3) ◽  
Author(s):  
N.A. Hasan ◽  
Mohd Y. Rafii ◽  
Harun A. Rahım ◽  
F. Ahmad ◽  
Nik Ismail
Keyword(s):  
Resistance Genes ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Blight Resistance ◽  
Rice Varieties ◽  
Research Article

scholarly journals Karakter Agronomi dan Ketahanan Beberapa Galur Pelestari Dihaploid terhadap Hawar Daun Bakteri

2016 ◽  
Vol 17 (2) ◽  
pp. 88
Author(s):  
Iswari S. Dewi ◽  
Indrastuti A. Rumanti ◽  
Bambang S. Purwoko ◽  
Triny S. Kadir
Keyword(s):  
Plant Height ◽  
Seed Weight ◽  
Bacterial Blight ◽  
Leaf Blight ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Agronomic Characters ◽  
Male Sterile ◽  
Wet Season ◽  
Productive Tiller

<p>Agronomic Characters and Resistance of Several Dihaploid Maintainer Lines to Bacterial Leaf Blight. Bacterial blight (Xanthomonas oryzae pv. oryzae, Xoo) is one of the most serious diseases of rice in Indonesia. From previous research thirteen lines of dihaploid (DH) maintainer lines-derived anther culture were selected for developing cytoplasmic male sterile lines. In this research those DH maintainers were evaluated for their agronomic characters such as plant height, number of productive tiller, and seed weight per hill as well as their resistance to Bacterial Leaf Blight (BLB) pathotypes III, IV and VIII at Indonesian Center Rice Research (ICRR), Sukamandi during wet season 2008/2009. The results showed that 10 DH maintainer lines i.e. BioMAc18-H36-3-Ma, BioMAc19-H36-3-Mb, BioMAc20- H36-3-Mc, BioMAc21-H36-4-M, BioMAc26-B1-1-Mb, BioMAc29-B2-1-Db, BioMAc31-B2-1-M, BioMAc33-B2-4- Pb, BioMAc34-B4-1-Da and BioMAc35-B4-1-Dc having plant height ranged from 88.79-104.08 cm, productive tiller ranged from 9 to 13 tillers/hill. Among those DH maintainer lines three lines were resistant to BLB pathotype III, i.e. BioMAc26-B1-1-Mb, BioMAc29-B2-1-Db and BioMAc31- B2-1-M lines, and two lines, i.e. BioMAc21-H36-4-M and BioMAc35-B4-1-Dc were highly resistant to BLB pathotype VIII. Only BioMAc35-B4-1-Dc lines highly resistant to BLB pathotype IV.</p><p> </p><p><strong>Abstrak</strong></p><p>Hawar daun bakteri yang disebabkan oleh bakteri Xanthomonas oryzae pv. oryzae, (Xoo) adalah salah satu penyakit utama padi di Indonesia. Dari penelitian sebelumnya 13 galur pelestari dihaploid (DH pelestari) yang berasal dari kultur antera telah diseleksi untuk perakitan galur mandul jantan baru. Tujuan penelitian ini adalah untuk mengevaluasi karakter agronomi dan ketahanan galur-galur DH pelestari terhadap patogen HDB. Karakter agronomi yang diamati meliputi tinggi tanaman, jumlah anakan produktif, dan bobot hasil per rumpun, sedangkan ketahanan terhadap HDB diamati berdasarkan skor ketahanan terhadap Xoo patotipe III, IV dan VIII di Balai Penelitian Padi, Sukamandi pada musim hujan 2008/2009. Hasil penelitian menunjukkan 10 galur DH pelestari, yaitu galur BioMAc18-H36-3-Ma, BioMAc19-H36-3-Mb, BioMAc20- H36-3-Mc, BioMAc21-H36-4-M, BioMAc26-B1-1-Mb, BioMAc29-B2-1-Db, BioMAc31-B2-1-M, BioMAc33-B2-4- Pb, BioMAc34-B4-1-Da, dan BioMAc35-B4-1-Dc mempunyai tinggi tanaman berkisar antara 88,79-104,08 cm, anakan produktif berkisar antara 9-13 batang/rumpun. Di antara galur DH pelestari yang tahan terhadap HDB patotipe III, yaitu galur BioMAc26-B1-1-Mb, BioMAc29-B2-1-Db dan BioMAc31- B2-1-M, dua galur yaitu galur BioMAc21-H36-4-M dan BioMAc35-B4-1-Dc sangat tahan terhadap HDB patotipe VIII, sedangkan galur BioMAc35-B4-1-Dc yang sangat tahan terhadap HDB patotipe IV.</p>

scholarly journals Screening of Rice Varieties for Bacterial Leaf Blight Resistance by Using SSR Markers

2015 ◽  
Vol 3 (1) ◽  
pp. 45-58
Author(s):  
T. H. Khan ◽  
F. Z. Evamoni ◽  
M. H. Rubel ◽  
K. M. Nasiruddin ◽  
M. Rahman
Keyword(s):  
Ssr Markers ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Blight Resistance ◽  
Rice Varieties

scholarly journals HERITABILITAS, SUMBER GEN, DAN DURABILITAS KETAHANAN VARIETAS PADI TERHADAP PENYAKIT HAWAR DAUN BAKTERI/ Heritability, Gene Resource, and Durability of Rice Varieties Resistance To Bacterial Leaf Blight Disease

2017 ◽  
Vol 36 (2) ◽  
pp. 99
Author(s):  
Dini Yuliani ◽  
Wage Ratna Rohaeni
Keyword(s):  
Genetic Resistance ◽  
Female Parent ◽  
Leaf Blight ◽  
Rice Production ◽  
Bacterial Leaf Blight ◽  
Sources Of Resistance ◽  
Rice Varieties ◽  
Resistant Varieties ◽  
Blight Disease ◽  
Resistant Progeny

<p>Bacterial leaf blight (BLB) disease is one of the obstacles in increasing of rice production. The use of resistant varieties is an effective and easy to implement for farmers. This paper discusses the heritability and source of resistance genes of rice varieties against the BLB disease and strategies to maintain the durability of resistant varieties as one of the control efforts through plant breeding to supports the increasing of rice production. Assembling and development of resistant varieties play an important role in controlling BLB disease because it has a genetic resistance<br />mechanism that can be inherited to progeny level. Varieties with vertical resistance are easily broken by pathogens, so it is necessary to assembling of varieties with horizontal resistance. To obtain the resistant progeny to BLB disease in the assembly of varieties, the position of the resistant varieties should be played as a female parent that has a high specific joining power. The nature of resistance to BLB is from a population whose parent genes are derived from multiple cross results has higher heritability. The populations derived from a double-cross have multigenic resistance and have the potential to produce recombinant individuals resistant for prolonged periods (durable). The availability of durable resistant varieties become a key requirement in sustainable BLB disease control. This matter can be done by improving the resistance of varieties through the assembling of varieties with various sources of resistance such as wild rice, local rice, and introduced rice.</p><p>Keywords: Rice, varieties, resistance, bacterial leaf blight, durability, heritability</p><p> </p><p><strong>ABSTRAK</strong></p><p>Penyakit hawar daun bakteri (HDB) merupakan salah satu kendala dalam peningkatan produksi padi. Penggunaan varietas tahan merupakan cara pengendalian yang efektif dan mudah diterapkan petani. Tulisan ini membahas heritabilitas dan sumber gen ketahanan varietas padi terhadap penyakit HDB dan strategi mempertahankan durabilitas varietas tahan sebagai salah satu upaya pengendalian melalui pemuliaan tanaman mendukung upaya peningkatan produksi padi. Perakitan dan pengembangan varietas tahan berperan penting mengendalikan penyakit HDB, karena memiliki mekanisme ketahanan genetik yang dapat diwariskan kepada keturunannya. Varietas dengan ketahanan vertikal mudah dipatahkan oleh patogen, sehingga perlu upaya perakitan varietas dengan ketahanan horizontal. Untuk memperoleh keturunan tanaman padi yang tahan terhadap penyakit HDB dalam perakitan varietas, posisi tetua tahan sebaiknya diperankan sebagai tetua betina yang memiliki daya gabung khusus yang tinggi. Sifat ketahanan HDB dari populasi tetua yang mengandung gen dari hasil silang ganda memilliki heritabilitas lebih tinggi. Populasi turunan dari silang ganda memiliki ketahanan multigenik dan berpeluang menghasilkan individu rekombinan tahan untuk periode yang lama (durable). Ketersediaan varietas tahan yang durable menjadi syarat utama dalam pengendalian penyakit HDB secara berkelanjutan. Hal ini dapat dilakukan dengan perbaikan ketahanan varietas melalui perakitan varietas dengan berbagai sumber ketahanan, di antaranya padi liar, padi lokal, dan padi introduksi.</p><p>Kata kunci: Padi, varietas, ketahanan, hawar daun bakteri, durabilitas, heritabilitas</p>

scholarly journals Development of New Rice (Oryza. sativa L.) Breeding Lines through Marker-Assisted Introgression and Pyramiding of Brown Planthopper, Blast, Bacterial Leaf Blight Resistance, and Aroma Genes

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2525
Author(s):  
Xuan Wang ◽  
Xinying Guo ◽  
Xixi Ma ◽  
Liang Luo ◽  
Yaoyu Fang ◽  
...  
Keyword(s):  
High Resistance ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Brown Planthopper ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Breeding Method ◽  
Breeding Lines ◽  
Biotic Stresses ◽  
Multiple Genes

Brown planthopper, blast, and bacterial blight are the main biotic stressors of rice and can cause a massive loss in rice production. Aroma is an important character of rice quality. It is of far-reaching significance to breed resistant and high-quality varieties using germplasms with objective genes. In this study, the introgression and pyramiding of brown planthopper (BPH), blast, and bacterial leaf blight (BLB) resistance genes and aroma genes into elite rice maintainers and restorers were conducted through conventional cross-breeding coupled with the marker-assisted selection (MAS) breeding method. Single-plant selection was performed from F2 onwards to select desirable recombinants possessing alleles of interest with suitable phenotypes. Respective linked markers were used in each generation from intercrossing to the F7 generation for tracking the presence of targeted genes. A total of 74 improved lines (ILs) have been developed which possess a combination of 1 to 4 genes for BPH, blast, and BLB resistance and aroma. These ILs showed moderate to high resistance to multiple biotic stresses (BPH, blast and BLB) or aromatic fragrance without obvious negative effects on agronomic traits. As multiple resistance and aromatic traits have become significant objectives in rice breeding, these resistance and/or aroma gene introgressed or pyramided lines have important application prospects. Core ideas: (1) marker-assisted breeding was used to pyramid multiple genes for an elite breeding line; (2) improved lines with the introgression of 1–4 genes were developed to achieve high resistance against various biotic stresses and aroma; (3) new lines were used as donor parents to introgress multiple genes in other genetic backgrounds.

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