scholarly journals PENGENDALIAN PENYAKIT LAYU BAKTERI NILAM MENGGUNAKAN PSEUDOMONAD FLUORESEN

2020 ◽  
Vol 11 (1) ◽  
pp. 19
Author(s):  
NASRUN NASRUN ◽  
CHRISTANTI CHRISTANTI ◽  
TRIWIDODO ARWIYANTO ◽  
IKA MARISKA
Keyword(s):  
Plant Growth ◽  
Bacterial Wilt ◽  
Block Design ◽  
Antagonistic Activity ◽  
Wilt Disease ◽  
Plant Production ◽  
Fluorescent Pseudomonad ◽  
Bacterial Wilt Disease ◽  
Pogostemon Cablin

<p>ABSTRAK<br />Penelitian pengendalian penyakit layu bakteri nilam (Ralstonia<br />solanacearum) menggunakan pseudomonad fluoresen di kebun petani<br />nilam Desa Situak Pasaman Barat, Sumatera Barat telah dilakukan pada<br />bulan Oktober 2003 sampai dengan Juni 2004. Penelitian ini bertujuan<br />untuk mendapatkan pseudomonad fluoresen yang berpotensi untuk<br />mengendalikan penyakit layu bakteri, dan meningkatkan pertumbuhan<br />dan produksi nilam. Isolat pseudomonad fluoresen Pf 63, Pf 90, Pf 91, Pf<br />147, dan Pf 180 sebagai perlakuan diisolasi dari rizosfer nilam sehat, dan<br />diseleksi  berdasarkan  kemampuan  antagonistik  terhadap  R.<br />solanacearum secara in vitro di Laboratorium Bakteriologi Tumbuhan<br />Fakultas Pertanian UGM. Isolat pseudomonad fluoresen tersebut<br />diintroduksikan ke nilam dan diadaptasikan selama 1 minggu sebelum<br />ditanam. Tanaman yang telah diperlakukan dengan isolat pseudomonad<br />fluoresen ditanam pada kebun yang telah terinfeksi dengan patogen pada<br />bulan Oktober 2003. Percobaan disusun dalam rancangan acak kelompok<br />(RAK) dengan 6 ulangan. Parameter pengamatan adalah masa inkubasi,<br />intensitas penyakit, pertumbuhan tanaman dan produksi minyak nilam.<br />Hasil penelitian menunjukkan bahwa isolat pseudomonad fluoresen<br />dapat mengendalikan penyakit layu bakteri dengan perpanjangan masa<br />inkubasi 6-52 hari dan penekanan intensitas penyakit 31,11 – 50,56%.<br />Disamping itu isolat pseudomonad fluoresen dapat mempengaruhi<br />peningkatan pertumbuhan tanaman yaitu tinggi tanaman (6,7 – 26,3 cm),<br />jumlah daun (4,6 – 30,1 daun/tanaman) dan berat kering daun (24,5 –<br />154,3 g/tanaman), dan produksi minyak nilam terutama jumlah minyak<br />(4,8 – 22,3 ml/tanaman). Hasil percobaan ini menunjukkan bahwa isolat<br />Pf 91 mempunyai kemampuan antagonistik tertinggi dalam mengen-<br />dalikan penyakit layu bakteri di lapangan.<br />Kata kunci : Nilam, Pogostemon cablin Benth, penyakit layu, bakteri,<br />pengendalian hayati, pseudomonad fluoresen</p><p><br />ABSTRACT<br />Controlling bacterial wilt disease on patchouli plant with<br />fluorescent pseudomonad<br />The study of controlling bacterial wilt disease on patchouli plant<br />(Ralstonia solanacearum) with fluorescent pseudomonad was carried out<br />in a farmer’s field in Situak Village West Pasaman, West Sumatera from<br />October 2003 to June 2004. The aims of the study were to find out the<br />effectiveness of fluorescent pseudomonad for controlling bacterial wilt<br />disease, increasing plant growth and production. Isolates of fluorescent<br />pseudomonad Pf 63, Pf 90, Pf 91, Pf 147 and Pf 180 as treatments were<br />isolated from the rhizosphere of healthy patchouli plant, and selected<br />based on antagonistic activity on R. solanacearum in vitro at the<br />Laboratory of Plant Bacteriology, Faculty of Agriculture, UGM. The<br />isolates were inoculated on patchouli plant and adapted for one week<br />before planting. The plants treated with fluorescent pseudomonad<br />isolates were planted in the field infected with pathogen on October<br />2003. The experiment was arranged in a randomized block design<br />(RBD) with six replications. The assessment parameters were incubation<br />period, disease intensity, plant growth and production of patchouli plants.<br />The results showed that fluorescent pseudomonad isolates could control<br />the bacterial wilt disease and delay the incubation period 6-52 days and<br />decrease the disease intensity 31,11–50,56%. In addition fluorescent<br />pseudomonad isolates could affect the increase of plant growth, i.e. plant<br />height ( 6,7 – 26,3 cm ), leaf numbers (4,6 – 30,1 leaves/plant) and dry<br />weight of leaves (24,5 – 154,3 g/plant), and plant production, especially<br />oil content (4,8 – 22,3 ml/plant). The results of the experiment showed<br />that Pf 91 isolate had the highest antagonistic activity on controlling the<br />bacterial wilt disease on field.<br />Key words : Patchouli, Pogostemon cablin Benth, wilt disease,<br />bacterial, biological control, fluorescent pseudomonad</p>

scholarly journals PENGENDALIAN PENYAKIT LAYU BAKTERI NILAM MENGGUNAKAN Bacillus spp. DAN Pseudomonad fluoresen

2020 ◽  
Vol 15 (3) ◽  
pp. 116
Author(s):  
CHRISNAWATI CHRISNAWATI ◽  
NASRUN NASRUN ◽  
TRIWIDODO ARWIYANTO
Keyword(s):  
Plant Growth ◽  
Bacterial Wilt ◽  
Block Design ◽  
Wilt Disease ◽  
Fluorescent Pseudomonad ◽  
In Planta ◽  
Bacterial Wilt Disease ◽  
Pogostemon Cablin ◽  
Bacillus Spp

<p>ABSTRAK</p><p>Penelitian pengendalian penyakit layu bakteri nilam (Ralstoniasolanacearum) menggunakan Bacillus spp. dan Pseudomonad fluoresen dikebun petani nilam di Nagari Kajai, Pasaman Barat, Sumatera Barat telahdilakukan pada bulan Mei sampai November 2006. Penelitian ini bertujuanuntuk mendapatkan Bacillus spp. dan Pseudomonad fluoresen yangberpotensi untuk mengendalikan penyakit layu bakteri, dan meningkatkanpertumbuhan dan produksi nilam. Isolat Bacillus spp. Bc 26; Bc 80 dan Bc81 dan Pseudomonad fluoresen Pf 101; Pf146 dan Pf 170 dalam bentukkombinasi sebagai perlakuan yang diisolasi dari rizosfer nilam sehat, dandiseleksi berdasarkan kemampuan antagonistik terhadap R. solanacearumsecara in vitro di laboratorium dan in planta di rumah kaca KP BalittroLaing Solok. Isolat Bacillus spp. dan Pseudomonad fluoresen tersebutdiintroduksikan ke nilam dan dibiarkan selama 1 minggu sebelum ditanam.Tanaman yang telah diperlakukan dengan isolat Bacillus spp. danPseudomonad fluoresen ditanam pada kebun nilam yang telah terinfeksioleh bakteri patogen pada bulan Mei 2006. Perlakuan yang diuji disusundalam rancangan acak kelompok (RAK) dengan 3 ulangan. Parameterpengamatan adalah perkembangan penyakit layu bakteri meliputi masainkubasi dan intensitas penyakit, pertumbuhan dan produksi tanaman.Hasil penelitian menunjukkan bahwa kombinasi isolat Bacillus spp. Bc26dan Pseudomonad fluoresen Pf101 dapat mengendalikan penyakit layubakteri nilam lebih baik dibandingkan dengan isolat Bacillus spp. Bc 26dan Pseudomonad fluoresen Pf 101 secara terpisah dan isolat Bacillus spp.dan Pseudomonad fluoresen lainnya secara kombinasi dan terpisah.Kombinasi isolat Bacillus spp. Bc 26 dan Pseudomonad fluoresen Pf 101dapat menunda masa inkubasi gejala penyakit layu bakteri dari 21 harisetelah tanam (HST) menjadi 63 HST dan menekan intensitas penyakitlayu bakteri dari 63,90% menjadi 14,67%. Di samping itu kombinasikedua isolat tersebut dapat meningkatkan pertumbuhan tanaman sepertitinggi tanaman dari 35,53 cm menjadi 52,77 cm, jumlah daun total dari32,00 daun/tanaman menjadi 104,67 daun/tanaman, jumlah tunas dari10,33 tunas/tanaman menjadi 25,33 tunas/tanaman, berat basah daun dari16,20 g/petak menjadi 81,73 g/petak dan berat kering daun dari 5,44 g/petak menjadi 27,15 g/petak. Hasil percobaan ini menunjukkan bahwakombinasi isolat Bacillus spp. Bc 26 dan Pseudomonad fluoresen Pf 101mempunyai kemampuan tertinggi dalam mengendalikan penyakit layubakteri dan meningkatkan pertumbuhan tanaman nilam di lapang.</p><p>Kata kunci: Pogostemon  cablin  Benth,  penyakit  layu  bakteri,pengendalian, Bacillus spp., Pseudomonad fluorescent</p><p>ABSTRACT</p><p>Use of Bacillus sp. and Fluorecent Pseudomonad to Control Bacterial Wilt Disease on Patchouli Plant</p><p>The study of controlling bacterial wilt disease on patchouli plant(Ralstonia solacearum) with Bacillus spp. and Fluorescent pseudomonadwas carried out in farmer field in Kajai Village, West Pasaman, WestSumatra from May to November 2006. The aims of the study were to findout the effectiveness of Bacillus spp. and Fluorescent pseudomonad forcontrolling bacterial wilt disease, and increasing plant growth andproduction. Isolates of Bacillus spp. Bc 26, Bc 80, and Bc 81, andFluorescent pseudomonad Pf 101, Pf 146 and Pf 170 in combination orseperation as treatments were isolated from the rhizosphere of healthypatchouli plant, and selected based on antagonistic activity on R.solanacearum in vitro at the laboratory and in planta at green house of KP.Balittro Laing Solok. Isolates were inoculated on patchouli plant andremained for one week before planting. The plants, treated with Bacillusspp. and Fluorescent pseudomonad isolates, were planted in the fieldinfected with pathogen bacterial in May 2006. The treatment was arrangedin a randomized block design (RBD) with three replications. Theassessment parameters were incubation period, disease intensity, plantgrowth and production of patchouli plants. The results showed thatcombination of Bacillus spp. and Fluorescent pseudomonad could controlthe bacterial wilt disease better than Bacillus spp. Bc 26 and Fluorescentpseudomonad seperately, and the other Bacillus spp. and Fluorescentpseudomonad either in combination or separation. Combination ofBacillus spp. Bc26 and Fluorescent pseudomonad Pf 101 delayed theincubation period from 21 to 63 days and decreased the disease intensity ofbacterial wilt from 63.90 to 14.67%. In addition combination of bothisolates could affect the increase of plant growth, i.e plant height from35.53 to 52.77 cm, total numbers of leaves from 32.00 to 104 leaves/plant,budding numbers from 10.33 to 25.33 budding/plant, wet weight of leavesfrom 16.20 to 81.73 g/plot, and dry weight of leaves from 5.44 to 27.15g/plot. The results of the experiment showed that Bacillus spp. Bc 26 andFluorescent pseudomonad Pf 101 isolates have the highest activity oncontrolling the bacterial wilt disease and increase the growth of patchouliplant in the field.</p><p>Key words: Patchouli, Pogostemon cablin Benth, bacterial wilt disease,biological control, Bacillus spp., Fluorescent pseudomonad</p>

scholarly journals PENGARUH PENGGUNAAN SUMBER PUPUK KALIUM TERHADAP PRODUKSI DAN MUTU MINYAK TANAMAN NILAM

2020 ◽  
Vol 18 (2) ◽  
pp. 60
Author(s):  
M. SYAKIR ◽  
GUSMAINI GUSMAINI
Keyword(s):  
Plant Growth ◽  
Block Design ◽  
Wilt Disease ◽  
Aromatic Plant ◽  
Bacterial Wilt Disease ◽  
Potassium Sources ◽  
Randomized Block Design ◽  
Pogostemon Cablin ◽  
Cultivation Technology

<p>ABSTRAK<br />Tanaman nilam (Pogostemon cablin Benth) sudah lama dibudi-<br />dayakan, namun produktivitas dan mutu minyak yang dihasilkan masih<br />rendah. Rendahnya produktivitas dan mutu minyak tersebut antara lain<br />disebabkan teknologi budidaya yang masih sederhana, dan berkembangnya<br />penyakit, seperti penyakit layu bakteri dan budog, serta hama yang<br />disebabkan oleh nematoda. Penelitian ini bertujuan untuk memperoleh<br />sumber dan dosis kalium yang tepat dalam meningkatkan pertumbuhan<br />dan produksi tanaman nilam. Kegiatan ini merupakan penelitian lapang<br />yang dilakukan di Kuningan, Jawa Barat, dari bulan Januari sampai<br />Desember 2009. Percobaan disusun menggunakan rancangan acak<br />kelompok lengkap, 9 perlakuan dengan 3 ulangan. Perlakuan terdiri atas<br />1) kontrol, 2) dosis KCl 60 kg/ha, 3) dosis KCl 120 kg/ha, 4) dosis KCl<br />180 kg/ha, 5) dosis KCl 240 kg/ha, 6) dosis K 2 SO 4 60 kg/ha, 7) dosis<br />K 2 SO 4 120 kg/ha, 8) dosis K 2 SO 4 180 kg/ha, 9) dosis K 2 SO 4 240 kg/ha.<br />Hasil penelitian menunjukkan bahwa sumber dan dosis kalium secara<br />nyata meningkatkan pertumbuhan dan produksi dibandingkan dengan<br />kontrol. Pertumbuhan tanaman, produksi terna kering, kadar dan produksi<br />minyak nilam terbaik ditunjukkan oleh perlakuan KCl atau K 2 SO 4 dengan<br />dosis 60 kg/ha. Serapan hara N dan P yang tertinggi ditunjukkan oleh<br />perlakuan pemberian 60 kg K 2 SO 4 /ha dan serapan hara K tertinggi pada<br />perlakuan 120 kg KCl/ha.<br />Kata kunci : Pogostemon cablin Benth, kalium, sumber, dosis, produksi,<br />patchouli alkohol</p><p>ABSTRACT<br />Effect of potassium sources on application yield and<br />quality of patchouli<br />Patchouli (Pogostemon cablin Benth) is an aromatic plant that has<br />long been cultivated in Indonesia, however its productivity and quality are<br />still low due to simple cultivation technology, and the development of<br />diseases, such as bacterial wilt disease, budog, and pests caused by<br />nematodes. This study aimed at obtaining sources and dosage of potassium<br />fertilizers to increase plant growth and oil yield of patchouli. The research<br />was conducted in Kuningan, West Java, from May to December 2009 and<br />was arranged using randomized block design, with 9 treatments and 3<br />replicates. There were 9 treatments consisting of : 1) control, 2) 60 kg<br />KCl/ha, 3) 120 kg KCl/ha, 4) 180 kg KCl/ha, 5) 240 kg KCl/ha, 6) 60 kg<br />K 2 SO 4 /ha, 7) 120 kg K 2 SO 4 /ha, 8) 180 kg K 2 SO 4 /ha, and 9) 240 kg<br />K 2 SO 4 /ha. The research results showed that the sources and dosage of<br />potassium fertilizers significantly affected growth, fresh herbal yield and<br />patchouli oil. The best plant growth, dry herbage yield, content and yield<br />of patchouli oil were obtained from the treatment of 60 kg/ha of KCl or<br />K 2 SO 4 . The highest N and P uptakes were shown by 60 kg K 2 SO 4 /ha<br />treatment and the highest K nutrient uptake was shown by 120 kg KCl/ha.<br />Key words : Pogostemon cablin Benth, potassium, source, dosage, yield,<br />patchouli olcohol</p>

scholarly journals MEKANISME ANTIBIOSIS BACILLUS SUBTILIS B315 UNTUK PENGENDALIAN PENYAKIT LAYU BAKTERI KENTANG

2015 ◽  
Vol 15 (1) ◽  
pp. 64 ◽  
Author(s):  
Nur Prihatiningsih ◽  
Triwidodo Arwiyanto ◽  
Bambang Hadisutrisno ◽  
Jaka Widada
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Leaf Area ◽  
Incubation Period ◽  
Bacterial Wilt ◽  
Wilt Disease ◽  
Disease Index ◽  
Growth Promoter ◽  
Bacterial Wilt Disease

Antibiosis mechanism of Bacillus subtilis B315 for controlling potato bacterial wilt disease. Bacillus subtilis B315 isolated from rhizospheric potato has antibiosis mechanism against Ralstonia solanacearum in vitro and become potentially used as controlling method of bacterial wilt in the field. The objectives of this research were to study the mechanism of B.subtilis B315 in controlling bacterial wilt disease, to study of B. subtilis B315 potency as both biocontrol and plant growth promoter, and to evaluate the mechanism as biocontrol agent. This green house experiment used CRD (Completely Randomized Design) with 5 treatments and 6 replicates. The treatments were control (without B. subtilis B315), B. subtilis B315 wild type, antibiosis mutant M16, antibiosis mutant M4, and antibiosis mutant M14. Variables observed were incubation period, disease index, infection rate, effectiveness of control, and growth components (i.e number of bud, plant height, leaf area, plant fresh and dry weight). The result of this research showed that B. subtilis B315 could delay incubation period, suppressed the disease index up to 64,9% and could promote the plant growth (leaf area). B. subtilis B315 had the antibiosis and other mechanisms that induced sistemic resistance. The implication of this research was that B. subtilis B315 could be used for biocontrol the bacterial wilt and promoted the potato growth.

scholarly journals Streptomyces sp.: Characterization, Identification and Its Potential as a Ralstonia solanacearum Biological Control Agent in vitro

2019 ◽  
Vol 2 (3) ◽  
pp. 89-96 ◽  
Author(s):  
Rachmad Saputra ◽  
Triwidodo Arwiyanto ◽  
Arif Wibowo
Keyword(s):  
Biological Control ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Biological Control Agent ◽  
Wilt Disease ◽  
Molecular Characteristics ◽  
Streptomyces Sp ◽  
Bacterial Wilt Disease ◽  
Wide Range

Streptomyces sp. bacteria have the potential to produce antibiotic compounds, which are one of the mechanisms that are widely used in biological control. However, in general, biological control mechanisms also occur through competition, cell wall degradation and induced resistance. This study was aimed to determine the physiological, biochemical and molecular characteristics of two isolates of Streptomyces sp. (S-4 and S16 isolates) isolated from the tomatoes roots, and to find out their ability to control Ralstonia solanacearum, which causes bacterial wilt disease on a wide range of hosts. The results showed both Streptomyces sp. isolates had several different physiological and biochemical characteristics and had a different ability to inhibit R. solanacearum in vitro. Streptomyces sp. S-16 isolate had a high similarity with Streptomyces diastaticus subsp. ardesiacus strain NRRL B-1773T based on the molecular identification results. Further research needs to be done to see the potential inhibition of the two Streptomyces isolates in inhibiting the development of bacterial wilt disease in tomato plants caused by R. solanacearum.

scholarly journals Role of Rhizobacteria Associated with Diseased Tomato Plants towards their Response with Ralstonia sp The Bacterial Wilt Agent

2020 ◽  
Vol 5 (4) ◽  
pp. 283-291
Author(s):  
Shraddha P. Mishra ◽  
Anfal Arshi ◽  
Ankur Agarwal ◽  
Merwyn P. Raj ◽  
Madhu Bala
Keyword(s):  
Bacterial Wilt ◽  
Agar Plate ◽  
In Vitro Study ◽  
Antagonistic Activity ◽  
Bacterial Strains ◽  
Tomato Crop ◽  
Bacterial Wilt Disease ◽  
Ralstonia Pseudosolanacearum ◽  
Different Strains

In this study, we have aimed to determine the antagonistic effects of various rhizobacteria against representatives of bacterial wilt disease; Ralstonia pseudosolanacearum with a comparison from control sterile distilled water (SDW) aliquots. During in-vitro study, bacterial wilt agent was clearly inhibited in their growth by significant percentage by 32 different strains through perforated agar plate method. Results showed 10 potential strains among those 32 strains viz. DIB76/BCS-8, DIB76/BCS-9, DIB76/BCS-10, DIB76/BCS-12, DIB76/BCS-19, DIB76/BCS-20, DIB76/BCS-21, DIB76/BCS-24, DIB76/BCS-26 and DIB76/BCS-27 had the highest growth suppression for 9 different strains of R. pseudosolanacearum as compared to other bacterial strains. Trials with tomato crop at polyhouse had no incidence of bacterial wilt than those for control. Study, thus identifies 10 rhizobacterial strains from rhizosphere soil of diseased tomato plant possessing potential antagonistic activity against the wilt pathogen and has prospects as good biocontrol agents.

scholarly journals Activity test of Bacillus Spp against bacterial wilt (R. solanacearum) on tomatoes by in vitro

2021 ◽  
Vol 883 (1) ◽  
pp. 012027
Author(s):  
G N C Tuhumury ◽  
J V Hasinu ◽  
H Kesaulya
Keyword(s):  
Bacterial Wilt ◽  
Pathogenic Bacteria ◽  
Wilt Disease ◽  
Tomato Plants ◽  
Bacterial Wilt Disease ◽  
Activity Test ◽  
Resistant Varieties ◽  
Bacillus Spp ◽  
Chemical Pesticides

Abstract Ralstonia solanocearum is a pathogenic bacteria that attacks tomatoes and causes wilt disease. Many efforts have been made to control this disease through cultivation, use of chemical pesticides, and development of resistant varieties, but bacterial wilt disease remains a serious problem economically. Nowadays, many biological controls are being developed using microbes. The use of Bacillus spp as an unfriendly microbe is very potential to control because it has pathogenic inhibitory activity. This study aims to obtain bacterial isolates of Bacillus spp which can suppress the development of bacterial wilt disease in tomato plants. The research was conducted in vitro at the Laboratory of Plant Physiology in the Agriculture Faculty, Unpatti. The results showed that Bacillus niabensis strain PT-32-1 and Bacillus subtilis strain SW116b could inhibit Ralstonia solanacearum, wilt disease in tomato plants in vitro.

scholarly journals Indigenous Bacillus spp. ability to growth promoting activities and control bacterial wilt disease (Ralstonia solanacearum)

2017 ◽  
Vol 18 (4) ◽  
pp. 1562-1567
Author(s):  
YULMIRA YANTI ◽  
TRIMURTI HABAZAR ◽  
REFLINALDON REFLINALDON ◽  
CHAINUR RAHMAN NASUTION ◽  
SRIMANO FELIA
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Wilt Disease ◽  
Subtilis Strain ◽  
Bacillus Sp ◽  
Bacterial Wilt Disease ◽  
Bacillus Spp ◽  
Growth Promoting ◽  
And Control

Yanti Y, Habazar T, Reflinaldon, Nasution CR, Felia S. 2017. Indigenous Bacillus spp. ability to growth promoting activities and control bacterial wilt disease (Ralstonia solanacearum). Biodiversitas 18: 1562-1567. Among the species of Plant Growth Promoting Rhizobacteria, Bacillus spp. has been found to provide beneficial effects to different plant species. Based on our previous research from in planta screening’s method, we found six indigenous strains of Bacillus spp., which had the ability to control bacterial wilt and increased growth and yields of chili. Those were Bacillus subtilis BSn5,q Bacillus sp. RD-AZPVI-03, Bacillus sp. Y81-1, B.cereus strain C38/15, B.cereus strain APSB-03, and B. subtilis strain CIFT-MFB-4158A. This research aimed to characterize the mechanism of selected Bacillus spp. as biocontrol agents of R. solanacearum and as biofertilizer of chili in vitro. We have observed the biocontrol characters (production of HCN, siderophore, biosurfactant, protease, root colonization, and antibiotic), and biofertilizer’s characters (production of Indole Acetic Acid (IAA), ammonia and phosphate solubilization) of Bacillus spp. All strains had ability to produce variable concentrations of IAA, B.subtilis strain CIFT-MFB-4158A can produce siderophore, all isolates cannot produce HCN and biosurfactant, 4 isolates can produce protease. Based on the ability to colonize roots, B.subtilis strain CIFT-MFB-4158A had the best ability to colonize root surface (4.17 x 105 CFU/g root), while B. subtilis BSn5 had the best ability to colonize internal roots (2.08 x 105 CFU/g), and 2 Bacillus strains can suppress R. solanacearum in vitro.

scholarly journals Potensi Limbah Media Jamur Konsumsi untuk Menekan Penyakit Layu Bakteri (Ralstonia solanacearum) pada Tanaman Kentang

Agrikultura ◽  
2015 ◽  
Vol 26 (2) ◽  
Author(s):  
Noor Istifadah ◽  
P. Rizky D. Sianipar
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Wilt Disease ◽  
Oyster Mushroom ◽  
Lentinus Edodes ◽  
Spent Mushroom Substrate ◽  
Organic Matters ◽  
Bacterial Wilt Disease ◽  
Auricularia Auricula

ABSTRACTThe Potential of Spent Mushroom Substrate to Control Bacterial Wilt Disease (Ralstoniasolanacearum) in PotatoBacterial wilt caused by Ralstonia solanacearum is one of important diseases in potato. One ofecofriendly-control measures is the use of organic matters including spent mushroom substrate.This paper discussed the study that examined the potential of spent substrates of oyster mushroom(Pleurotus sp.), shiitake (Lentinus edodes), Auricularia auricula and composted spent mushroomsubstrate to inhibit the growth of Ralstonia solanacearum in vitro and to suppress the diseaseincidence of bacterial wilt disease in potato. The results showed that spent substrate of shiitakemushroom slightly inhibited the growth of R. solanacearum in vitro with inhibition zone 1.5 mm.The spent mushroom substrate applied in the growth medium and drenching its water extractevery week significantly reduced the disease incidence of bacterial wilt disease, however thesuppression level was only 26.1%. The spent substrates of oyster mushroom (Pleurotus sp)., A.auricula and composted spent mushroom substrate did not suppress the disease incidence.Keywords : Lentinus edodes, Pleurotus, Auricularia auricula, organic matters, spent mushroomwater extractABSTRAKLayu bakteri yang disebabkan Ralstonia solanacearum merupakan salah satu penyakit penting padapertanaman kentang. Salah satu cara pengendalian penyakit yang ramah lingkungan adalahdengan menggunakan bahan organik, termasuk limbah media jamur konsumsi. Artikel inimendiskusikan penelitian yang mengkaji potensi limbah media tanam jamur tiram (Pleurotus sp.),jamur shiitake (Lentinus edodes), jamur kuping (Auricularia auricula) dan kompos limbah mediajamur konsumsi untuk menghambat R. solanacearum in vitro serta menekan penyakit layu bakteripada tanaman kentang. Hasil percobaan menunjukkan air rendaman limbah media jamur shiitakerelatif menekan R. solanacearum secara in vitro dengan zona penghambatan 1,5 mm. Limbahmedia jamur shiitake yang diaplikasikan pada medium tanam dan air rendamannya disiramkan ketanaman kentang setiap minggu sekali dapat menurunkan persentase tanaman layu secara nyatadibandingkan dengan kontrol, namun tingkat penekanannya hanya sebesar 26,1%. Limbah mediajamur tiram (Pleurotus sp.), jamur kuping (A. auricula) serta kompos limbah media jamur tidakmenghambat R. solanacearum secara in vitro dan tidak dapat menekan penyakit layu bakteri padatanaman kentang.Kata kunci : Lentinus edodes , Pleurotus, Auricularia auricula, bahan organik, air rendamanlimbah jamur

scholarly journals EVALUASI KETAHANAN HIBRIDA SOMATIK NILAM TERHADAP PENYAKIT LAYU BAKTERI (Ralstonia solanacearum)

2020 ◽  
Vol 15 (3) ◽  
pp. 110
Author(s):  
NASRUN NASRUN ◽  
NURMANSYAH NURMANSYAH ◽  
HERWITA IDRIS
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Somatic Hybrid ◽  
Bacterial Pathogen ◽  
Wilt Disease ◽  
Leaf Production ◽  
Fresh Leaf ◽  
Bacterial Wilt Disease ◽  
Green House ◽  
Pogostemon Cablin

<p>ABSTRAK</p><p>Penelitian evaluasi ketahanan hibrida somatik nilam terhadappenyakit layu bakteri (Ralstonia solanacearum) telah dilaksanakan dilaboratorium dan rumah kaca KP Laing Solok dari bulan Januari sampaiDesember 2007. Penelitian bertujuan untuk mendapatkan nomor hibridasomatik nilam yang tahan terhadap penyakit layu bakteri. Kegiatanpenelitian meliputi pengambilan sampel tanaman nilam terinfeksi penyakitlayu bakteri di lapangan, dan isolasi dan perbanyakan isolat bakteripatogen di laboratorium. Uji patogenisitas isolat bakteri patogen pada bibitnilam dilaksanakan di rumah kaca. Selanjutnya perbanyakan bibit nilamhibrida somatik, inokulasi bibit dengan bakteri somatik, dan inkubasi bibittersebut dilaksanakan di rumah kaca. Penelitian ini menggunakan beberapanomor nilam hibrida somatik sebagai perlakuan yang disusun dalamrancangan acak lengkap (RAL) dengan tiga ulangan. Parameter yangdiamati adalah masa inkubasi menunjukkan gejala awal penyakit untuk ujipatogenisitas isolat bakteri patogen. Masa inkubasi menunjukkan gejalaawal penyakit dan kematian serta intensitas penyakit. Selanjutnya jugadiamati pertumbuhan tanaman dan produksi daun basah untuk pengujianketahanan nomor hibrida somatik nilam terhadap bakteri patogen. Hasilpenelitian menunjukkan bahwa hibrida somatik 2IV/4 dan 9II/21 sertavarietas Girilaya sampai akhir pengamatan tidak menampakkan gejalapenyakit. Sementara itu hibrida somatik 2IV/6; 9II/34; 2IV/9; 9IV/3; dan9IV/6 menunjukkan gejala awal penyakit (118,5 – 133,6 hari setelahinokulasi ”HSI”) dan kematian bibit (130,5 – 182,2 HSI) paling lamadengan intensitas penyakit (29,6 – 48,2%) lebih rendah dibandingkandengan hibrida somatik lainnya. Selanjutnya, penyumbatan pembuluhkayu sangat rendah yaitu 5,20 – 9,50% pada bagian akar, tetapi padabagian pangkal batang dan pucuk tidak ada penyumbatan. Pertumbuhandan produksi daun basah lebih tinggi dibandingkan dengan hibrida somatiklainnya, yakni tinggi tanaman 78,00 – 104,00 cm; jumlah daun 66 – 112daun, dan produksi daun basah 55,00 - 99,19 g. Sebaliknya hibridasomatik 9IV/4; 9II/23; 9II/33; 9IV/14; dan 2IV/1 memperlihatkan gejalaawal penyakit (32,3 -105,84 HSI) dan kematian bibit nilam (41,5 – 125,0HSI) lebih cepat, dan intensitas penyakit lebih tinggi (75 – 100%)dibandingkan hibrida somatik lainnya termasuk varietas Sidikalang.Penyumbatan pembuluh kayu oleh bakteri patogen sebesar 12,46 – 31,25%pada bagian akar, 6,10 – 22,50% pada bagian batang, dan 4,17 – 6,25%pada pucuk. Begitu juga dengan pertumbuhan dan produksi daun basahlebih rendah dibandingkan dengan hibrida somatik lainnya, dengan tinggitanaman 42,20 – 61,85 cm, jumlah daun 24 - 37 daun, dan produksidaun basah 18,00 - 41,20 g. Berdasarkan hasil penelitian ini terpilih duanomor hibrida somatik yaitu (2IV/4 dan 9 II/21) yang tahan terhadappenyakit layu bakteri, dan lima nomor hibrida somatik yaitu (9IV/6, 9IV/3,9II/34, 2IV/9, dan 2IV/6) yang toleran terhadap penyakit layu bakteri dirumah kaca.</p><p>Kata kunci : Pogostemon cablin Benth., nilam, penyakit layu bakteri,evaluasi ketahanan, hibrida somatik</p><p>ABSTRACT</p><p>Evaluation on the Resistance of Hybrid Somatic PatchouliPlant to Bacterial Wilt Disease (Ralstonia solanacearum)</p><p>The study on the resistance evaluation of somatic hybrid patchouliplant to bacterial wilt disease (Ralstonia solanacearum) had beenconducted in green house KP.Laing Solok from January to December2008. The objective of this study was to find the somatic hybrid numbersof patchouli plants resistant to bacterial wilt disease. The studied activitieswere collection of patchouli plant that was infected by bacterial wiltdisease in the field. Isolation and culture of the pathogen bacterial wereconducted in the laboratory and virulence test of pathogen bacterial onpatchouli plant was in the green house. In addition, sub culture of thesomatic hybrid seeds of patchouli plants, inoculation of these seeds bybacterial pathogen, and incubation of them were done at green house. Thestudy used some numbers of somatic hybrid as treatments that werearranged in completely randomized design (CRD) with three replicates. Asparameters were incubation period showing the first disease symptom forpathogenicity test of bacterial pathogen. Incubation period showed thefirst symptom died plant and diseases intensity. Plant growth and leafproduction were also observed for the study of number resistance ofsomatic hybrid patchouli plant to bacterial pathogen. The results showedthat somatic hybrid 2 IV/4 and 9 II/21 and Girilaya varieties did not showany symptoms. However, somatic hybrid 2IV/6, 9II/34, 2IV/9, 9IV/3, and9IV/6 showed the first symptom (118.5 – 133.6 days after inoculation”DAI”) and dead period (41.5 – 125.0 DAI) were the longest and thedisease intensity (29.6 – 48.2%) was lower than other somatic hybrid.Occluded xylem vessel by pathogen bacterial on root was lowest 5.20 –9.50%, but it was not found on base and young stems. They are alsohigher in the growth and leaf production than another somatic hybridpatchouli plant with 75.00 – 104.00 cm plant height, 66 -112 leaves/plant,and fresh leaf production of 55.00 – 99.19 g/pot. However, somatic hybrid9 IV/4, 9 II/23, 9 II/33, 9 IV/14, and 2 IV/1 showed earlier diseasesymptom, quicker seed death and higher disease intensity (75 -100%) thanother somatic hybrid patchouli plants including Sidikalang variety. Inaddition they had occluded xylem by bacterial pathogen 12.46 - 31.25% onthe root, 6.10 - 22.50% on the stem, and 4.17 – 6.25% on young stem.Plant growth and leaf production were lower than other somatic hybridpatchouli plants as of was 42.20-61.85 cm plant height; 24-37 leaves/plant,and fresh leaf production 18.00-41.20 g/pot. According to these results,experiment selected two numbers of somatic hybrid (2IV/4 and 9II/21)which were resistant to bacterial wilt disease, and five numbers of somatichybrid (9 IV/6, 9IV/3, 9II/34, 2IV/9, and 2IV/6) that were tolerant tobacterial wilt disease in the glass house.</p><p>Key words: Pogostemon cablin Benth., patchouli, bacterial wilt disease,resistant evaluation, somatic hybrid patchouli plant</p>

scholarly journals SKRINING KETAHANAN SOMAKLON NILAM TERHADAPP PENYAKIT LAYU BAKTERI (Ralstonia solanacearum)

2016 ◽  
Vol 21 (3) ◽  
pp. 131
Author(s):  
S. Y. HARTATI ◽  
E. HADIPOENTYANTI ◽  
AMALIA AMALIA ◽  
NURSALAM NURSALAM
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Screening Method ◽  
Wilt Disease ◽  
Resistance Screening ◽  
Bacterial Wilt Disease ◽  
House Condition ◽  
Moderately Resistant

<p>ABSTRAK</p><p>Layu   bakteri  yang   disebabkan   oleh <em>  Ralstonia   solanacearum <br /> </em>merupakan salah satu penyakit penting pada tanaman nilam. Perakitan <br /> varietas nilam tahan terhadap penyakit tersebut yang dilakukan melalui <br /> induksi keragaman somaklonal telah menghasilkan beberapa somaklon <br /> yang tahan terhadap <em>R. solanacearum</em> secara <em>in-vitro</em>. Tujuan penelitian <br /> adalah menguji tingkat ketahanan somaklon tersebut terhadap penyakit <br /> layu  pada  kondisi  rumah  kaca (<em>in-vivo</em>).  Penelitian  disusun  dalam <br /> Rancangan  Acak  Lengkap  dengan 27  perlakuan, 3  ulangan,  dan 10 <br /> tanaman/ulangan. Sebagian akar dari somaklon nilam dilukai (dipotong), <br /> selanjutnya diinokulasi (disiram) dengan suspensi<em> R. solanacearum</em> dengan <br /> berbagai konsentrasi 10<sup>5</sup>, 10<sup>7</sup>, dan 10<sup>9 </sup><em>cfu</em>/ml, sebanyak 50 ml/tanaman. <br /> Hasil penelitian menunjukkan, bahwa somaklon yang diinokulasi dengan <br /> konsentrasi 10<sup>5 </sup><em>cfu</em>/ml, 50  ml/tanaman  semuanya  tidak  menunjukkan <br /> gejala layu. Somaklon yang diinokulasi dengan konsentrasi 107 dan 10<sup>9</sup></p><p><em>cfu</em>/ml,  50 ml/tanaman, sebagian layu dan mati.  Dari somaklon yang</p><p>7</p><p>diinokulasi dengan konsentrasi 10     <em>cfu</em>/ml, 50 ml/tanaman, 8 di antaranya</p><p>menunjukkan respon sangat tahan, 4 tahan, dan 5 agak tahan. Ke 17 <br /> somaklon tersebut mempunyai intensitas penyakit &lt;50% dan semua lebih <br /> tahan dari pada varietas Sidikalang (agak toleran). Dari 17 somaklon yang <br /> diinokulasi dengan konsentrasi 10<sup>9 </sup><em>cfu</em>/ml, 50 ml/tanaman, 2 di antaranya <br /> sangat tahan dan 7 somaklon tahan. Teknik skrining ini dapat digunakan <br /> sebagai  metode  standar  untuk pengujian  ketahanan  nilam  terhadap <br /> penyakit layu.</p><p>Kata kunci:  Skrining  ketahanan,  somaklon,  nilam,  penyakit  layu,<em>  R. solanacearum.</em></p><p><em> </em></p><p><em></em>ABSTRACT</p><p>Resistance-Screening of Patchouli Somaclones on Bacterial Wilt Disease (Ralstonia solanacearum) </p><p>Bacterial wilt caused by <em>Ralstonia solanacearum </em>is one of the most <br /> important  diseases  on patchouli.  The  developing patchouli resistance <br /> varieties against  wilt  disease  conducted  through the  induction  of <br /> somaclonal variation produced resistant patchouli somaclones against <em>R. <br /> </em><em>solanacearum </em>(in-vitro). The aim of this  research was to screen the <br /> resistance of those patchouli somaclones against wilt disease under a glass <br /> house condition (in-vivo). The research was conducted in a Randomized <br /> Completely Design  with 27 treatments, 3  replicates,  and 10  plants/ <br /> replicate. Some roots of the patchouli somaclones were wounded (cut), <br /> then inoculated (drenched)   with <em>  R.   solanacearum</em>  suspension   in <br /> concentration of 10<sup>5</sup>, 10<sup>7</sup>, and 10<sup>9 </sup><em>cfu</em>/ml; 50 ml/plant. The result showed, <br /> that all the patchouli somaclones inoculated with <em>R. solanacearum</em> 10<sup>5 <br /> </sup>cfu/ml, 50 ml/plant were not show any wilt sympthom. Whereas, some <br /> somaclones inoculated with the higher concentration 10<sup>7 </sup>and 109 cfu/ml, 50 ml/plant were wilted and died. Among the somaclones inoculated with  the concentration of 10<sup>7</sup>  cfu/ml, 50 ml/plant, 8 of them were highly  resistant, 4 were resistant, and 5 were moderately resistant. The disease  intencity of those 17 somaclones were &lt;50% and they were more resistant than  the  Sidikalang  variety  (moderately  tolerant).  Among  those  17 <br /> somaclones inoculated with the concentration of 10<sup>9 </sup>cfu/ml, 50 ml/plant, 2 <br /> of them were highly resistant and 7 were resistant. This screening method <br /> could be used as a standard protocol for patchouli resistance screening <br /> against wilt disease.</p><p>Kata kunci: Screening resistance, somaclone, patchouli, wilt disease, <em>R. solanacearum.</em></p>

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