scholarly journals Study of ELISA and antibiotic sensitivity test for Salmonella enteritidis as experimental infection in mice

2009 ◽  
Vol 6 (1) ◽  
pp. 114-122
Author(s):  
Baghdad Science Journal

Salmonella enteritidis one of more important as epidemiological bacteria between other salmonella types. It is very important pathologically that cause food poising and gastrointestinal tract infections. This study includes some of immunological changes that appear by ELISA test and antibiotic sensitivity test against these bacteria in mice. ELISA test results appears high immunological response happen after 3 days of inoculation, mean titration readings beginning 0.198 and the maximum mean titration after 15 days of inoculation 1.538 and begin to decrease after this time slowly to remain about 0.297 after 40 days of inoculation. An antibiotics sensitivity test result appears, this bacteria sensitive to Chloramphenicol, Ceftriaxone, Ciprofloxacin and Cotrimaxazol. Resistance to Neomycin, Streptomycin and Rifampicin, while intermediate against Ampicilin and Amoxicillin. Another test we use Vitek system to know bacterial sensitivity against to more another types of antibiotics and to confirm between some of them.

2014 ◽  
Vol 11 (2) ◽  
pp. 159-166 ◽  
Author(s):  
MM Rashid ◽  
MJ Ferdoush ◽  
M Dipti ◽  
P Roy ◽  
MM Rahman ◽  
...  

The foremost important goal of the present study was to investigate the bacteriological and pathological conditions in lungs of goats slaughtered in four different slaughter houses/places of Mymensingh Sadar, Mymensingh and in addition to it, antibiotic sensitivity test of commonly used antibiotics in Veterinary practices were performed on isolated bacteria. A total of 75 lungs of slaughtered goats were examined individually and out of which 20 affected lungs were collected for histopathology and bacterial isolation respectively from Mymensingh Sadar, Mymensingh in Bangladesh, during the period from January to May 2013.The lung lesions was grossly recorded 40% in goats (30 out of 75 lungs examined). Grossly, the lung lesions were categorized into (a) hemorrhages 35% (b) congestion 25% (c) hemorrhage and congestion 15% (d) emphysematous lung 15% and (e) hepatization in lung10%. In histopathology, lung lesions were categorized into (a) bronchopneumonia 30%, (b) pneumonia 25% (c) hemorrhagic pneumonia 20% (d) emphysema 15%, (e) purulent pneumonia 10%. Pasteurella sp. (15%) was isolated from the lung lesions of hemorrhagic pneumonia, E. coli. (25%) from bronchopneumonia and Staphylococcus sp. (40%) from purulent pneumonia, focal pneumonia and emphysema, and mixed infection (Staphylococcus sp. and E. coli) 20%. Finally antibiotics sensitivity test was performed on isolated bacteria to which ciprofloxacin was more powerful than others (penicillin, amoxicillin, streptomycin, nalidixic acid and kanamycin) tested and the second one was oxytetracyclin.DOI: http://dx.doi.org/10.3329/bjvm.v11i2.19142Bangl. J. Vet. Med. (2013).11(2): 159-166


Author(s):  
Anna Joy ◽  
Aparna Anand ◽  
Arathy R Nath ◽  
Meera S Nair ◽  
Dr. K. G. Prasanth

Antibiotics are one of the most commonly prescribed drugs today. Rational use of antibiotics is therefore extremely important as their injudicious use can adversely affect the patient. Drug Utilization Evaluation (DUE) is a system of ongoing systematic criteria based evaluation of drug that will help to ensure that medicines are used appropriately. It is drug/disease specific and can be structured so that it will assess the actual process of prescribing, dispensing, or administration of drug. The retrospective study was conducted At Pk Das Institute of Medical Sciences, Palakkad, Kerala for a duration of 6 months (February 2017 - January 2018). A source of data includes Patient case sheets &medication charts, nursing charts, culture & sensitivity reports. The inclusion criterion includes Patients aged between 18- 80 year, prescribed with oral and parenteral antibiotics. . On analyzing the gender, male gender (n= 111, 55.5%) were higher in numbers as compared to female counterparts (n=89, 44.5%). In our study the majority of the patients prescribed with antibiotics were with the clinical assessment of COPD (n=39, 19.5%), UTI (n=37, 18.5%) and LRTI (n=28, 14%), Bronchial asthma (n=19, 9.5%) respectively. On analyzing the data based on antibiotic sensitivity test, antibiotic test were performed and followed in (n=64,32%)prescriptions and in (n=47,23.5%)prescriptions were test is not followed respectively. In (n=89,44.5%) prescriptions, antibiotic sensitivity test is not performed. On analysis of antibiotics prescribed, the most commonly prescribed antibiotics were cephalosporins, of these ceftriaxone was highly prescribed of all (n=95). The high percentage of antibiotic prescriptions may indicate a high probability of irrational use. This study also point out irrational use of antibiotics are more leading to resistance, misuse and serious problems. So certain strategies should be put forward to strengthen rational use of antibiotics. Keywords: Antibiotics, Antibiotic Susceptibility Test, Irrational use, Resistance


Author(s):  
Subha Ganguly

The present article reports on the bacteriological examination and antibiotic sensitivity test of pus sample collected from an abscess at the neck region of a cow.


2020 ◽  
Vol 16 (2) ◽  
pp. 73
Author(s):  
Nurhafizah Rafiani ◽  
Renny Aditya ◽  
Noor Muthmainah

Abstract: A cesarean delivery increases the risk of wound infection it should be prevented by using antibiotics. This study was to determine the pattern of bacterial sensitivity in surgical wounds of cesarean section patients for selected antibiotics, i.e ceftriaxone, cefixime, sulbactam ampicillin, ciprofloxacin, clindamycin and gentamicin.This observational study was conducted at the Ulin Public Hospital in Banjarmasin from July to September 2019. Using a cross sectional approach Samples of bacteria were identidied from 36 that were pusposively sampled, i.e., Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli. Antibiotic sensitivity test showed that Staphylococcus aureus was sensitive against gentamicin (100%),whereas Staphylococcus epidermidis and Escherichia coli. Were intermediately sensitive towards gentamicin (62.5%) and ceftriaxone (80%) Keywords: Antibiotic susceptibility, caesarean section, surgical wound


1981 ◽  
Vol 5 (5) ◽  
pp. 243-245 ◽  
Author(s):  
T. McDonald ◽  
N. Pearson ◽  
F. Johnson ◽  
F. W. O'grady

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