scholarly journals Phenotypic and genotypic detection of metallo-β-lactamases in A. baumanii strains isolated in the teaching hospitals Shahrekord, southwest Iran

2019 ◽  
Author(s):  
Mansoor Khaledi ◽  
Milad Shahini Shams Abadi ◽  
Majid Validi ◽  
Behnam Zamanzad ◽  
Rezvan Vafapour ◽  
...  
Keyword(s):  
Continuous Monitoring ◽  
Antibiotic Sensitivity ◽  
Teaching Hospitals ◽  
Clinical Samples ◽  
Gene Detection ◽  
Biochemical Tests ◽  
Acinetobacter Baumanii ◽  
Antibiotic Sensitivity Test ◽  
Phenotypic Tests ◽  
Southwest Iran

Abstract Objective Acinetobacter baumanii is a pathogenic bacterium that is the cause of many nosocomial infections. This study aimed to determine metallo-β-lactamases produced by the A. baumanii strains isolated in the teaching hospitals in Shahrekord, southwest Iran. Results A total of 100 A. baumanii isolates were isolated from 250 clinical samples between June 2013 and June 2014. Then, the strains were identified by biochemical tests and MBL screening conducted by the phenotypic tests modified Hodge, EDTA-disk synergy (EDS), combined disk (CD) and AmpC disc after antibiotic sensitivity test, and the bla (IMP) and bla (VIM) genes detected by PCR. Eighty five (85%) isolates were resistant to meropenem and imipenem. Phenotypic tests showed that out of the 100 isolates, 46, 59, 50, 65 and 65 isolates were positive: AmpC disk, CD, EDS, Modified Hodge and Etest MBL, suggesting MBL production, respectively. Gene detection by PCR showed that 23 isolates carried the VIM-1 gene and only three isolates carried the IMP-1 gene. The prevalence of metallo-β-lactamases-containing A. baumanii isolates is increasing, and the coexistence of various carbapenemases is a problem. Continuous monitoring of the infections due to these bacteria should be taken into account in planning for alternative and new therapeutic strategies.

scholarly journals Phenotypic and genotypic detection of metallo-β-lactamases in A. baumanii isolates obtained from clinical samples in Shahrekord, southwest Iran

2019 ◽  
Author(s):  
Mansoor Khaledi ◽  
Milad Shahini Shams Abadi ◽  
Majid Validi ◽  
Behnam Zamanzad ◽  
Rezvan Vafapour ◽  
...  
Keyword(s):  
Continuous Monitoring ◽  
Antibiotic Sensitivity ◽  
Therapeutic Strategies ◽  
Clinical Samples ◽  
Gene Detection ◽  
Biochemical Tests ◽  
Acinetobacter Baumanii ◽  
Antibiotic Sensitivity Test ◽  
Phenotypic Tests ◽  
Southwest Iran

Abstract Objective: Acinetobacter baumanii is a pathogenic bacterium that is the cause of many nosocomial infections. This study aimed to determine metallo-β-lactamases (MBL) produced by the A . baumanii isolated obtained from clinical samples in Shahrekord, southwest Iran . Results: A total of 100 A . baumanii isolates were isolated from 250 clinical samples between June 2013 and June 2014. Then, the isolates were identified by biochemical tests and MBL screening conducted by the phenotypic tests modified Hodge, EDTA-disk synergy (EDS), combined disk (CD) and AmpC disc after antibiotic sensitivity test, and the bla genes detected by PCR. Eighty-five (85%) isolates were resistant to meropenem and imipenem. Phenotypic tests showed that out of the 100 isolates, 46, 59, 50, 65 and 65 isolates were positive: AmpC disk, CD, EDS, Modified Hodge and Etest MBL, suggesting MBL production, respectively. Gene detection by PCR showed that 23 isolates carried the VIM-1 gene and only three isolates carried the IMP-1 gene. The prevalence of metallo-β-lactamases-containing A . baumanii isolates is increasing, and the coexistence of various carbapenemases is a problem. Continuous monitoring of the infections due to these bacteria should be taken into account in planning for alternative and new therapeutic strategies.

scholarly journals Phenotypic and genotypic detection of metallo-β-lactamases in A. baumanii isolates obtained from clinical samples in Shahrekord, southwest Iran

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Mansoor Khaledi ◽  
Milad Shahini Shams Abadi ◽  
Majid Validi ◽  
Behnam Zamanzad ◽  
Rezvan Vafapour ◽  
...  
Keyword(s):  
Continuous Monitoring ◽  
Antibiotic Sensitivity ◽  
Therapeutic Strategies ◽  
Clinical Samples ◽  
Gene Detection ◽  
Biochemical Tests ◽  
Acinetobacter Baumanii ◽  
Antibiotic Sensitivity Test ◽  
Phenotypic Tests ◽  
Southwest Iran

Abstract Objective Acinetobacter baumanii is a pathogenic bacterium that is the cause of many nosocomial infections. This study aimed to determine metallo-β-lactamases (MBL) produced by the A. baumanii isolates obtained from clinical samples in Shahrekord, southwest Iran. Results A total of 100 A. baumanii were isolated from 250 clinical samples between June 2013 and June 2014. Then, the isolates were identified by biochemical tests, and MBL screening was conducted by the phenotypic tests modified Hodge, EDTA-disk synergy (EDS), combined disk (CD) and AmpC disc after antibiotic sensitivity test. Using PCR technique the bla genes were detected. Eighty-five (85%) isolates were resistant to meropenem and imipenem. Phenotypic tests showed that out of the 100 isolates, 46, 59, 50, 65 and 65 isolates were positive: AmpC disk, CD, EDS, Modified Hodge and E-test MBL respectively. Gene detection by PCR showed that 23 isolates carried the VIM-1 gene and only three isolates carried the IMP-1 gene. The prevalence of metallo-β-lactamases isolates containing A. baumanii is increasing. Furthermore, the coexistence of various carbapenemases is dominantly act as a major problem. Continuous monitoring of the infections related to these bacteria should be considered to plan an alternative and new therapeutic strategies.

scholarly journals Phenotypic and genotypic detection of metallo-β-lactamases in A. baumanii isolates obtained from clinical samples in Shahrekord, southwest Iran

2019 ◽  
Author(s):  
Mansoor Khaledi ◽  
Milad Shahini Shams Abadi ◽  
Majid Validi ◽  
Behnam Zamanzad ◽  
Rezvan Vafapour ◽  
...  
Keyword(s):  
Continuous Monitoring ◽  
Antibiotic Sensitivity ◽  
Therapeutic Strategies ◽  
Clinical Samples ◽  
Gene Detection ◽  
Biochemical Tests ◽  
Acinetobacter Baumanii ◽  
Antibiotic Sensitivity Test ◽  
Phenotypic Tests ◽  
Southwest Iran

Abstract Objective: Acinetobacter baumanii is a pathogenic bacterium that is the cause of many nosocomial infections. This study aimed to determine metallo-β-lactamases (MBL) produced by the A . baumanii isolated obtained from clinical samples in Shahrekord, southwest Iran . Results: A total of 100 A . baumanii isolates were isolated from 250 clinical samples between June 2013 and June 2014. Then, the isolates were identified by biochemical tests and MBL screening conducted by the phenotypic tests modified Hodge, EDTA-disk synergy (EDS), combined disk (CD) and AmpC disc after antibiotic sensitivity test, and the bla genes detected by PCR. Eighty-five (85%) isolates were resistant to meropenem and imipenem. Phenotypic tests showed that out of the 100 isolates, 46, 59, 50, 65 and 65 isolates were positive: AmpC disk, CD, EDS, Modified Hodge and Etest MBL, suggesting MBL production, respectively. Gene detection by PCR showed that 23 isolates carried the VIM-1 gene and only three isolates carried the IMP-1 gene. The prevalence of metallo-β-lactamases-containing A . baumanii isolates is increasing, and the coexistence of various carbapenemases is a problem. Continuous monitoring of the infections due to these bacteria should be taken into account in planning for alternative and new therapeutic strategies.

scholarly journals Phenotypic and genotypic detection of metallo-β-lactamases in A. baumanii isolates obtained from clinical samples in Shahrekord, southwest Iran

2019 ◽  
Author(s):  
Mansoor Khaledi ◽  
Milad Shahini Shams Abadi ◽  
Majid Validi ◽  
Behnam Zamanzad ◽  
Rezvan Vafapour ◽  
...  
Keyword(s):  
Continuous Monitoring ◽  
Antibiotic Sensitivity ◽  
Therapeutic Strategies ◽  
Clinical Samples ◽  
Gene Detection ◽  
Biochemical Tests ◽  
Acinetobacter Baumanii ◽  
Antibiotic Sensitivity Test ◽  
Phenotypic Tests ◽  
Southwest Iran

Abstract Objective: Acinetobacter baumanii is a pathogenic bacterium that is the cause of many nosocomial infections. This study aimed to determine metallo-β-lactamases (MBL) produced by the A . baumanii isolated obtained from clinical samples in Shahrekord, southwest Iran . Results: A total of 100 A . baumanii isolates were isolated from 250 clinical samples between June 2013 and June 2014. Then, the isolates were identified by biochemical tests and MBL screening conducted by the phenotypic tests modified Hodge, EDTA-disk synergy (EDS), combined disk (CD) and AmpC disc after antibiotic sensitivity test, and the bla genes detected by PCR. Eighty-five (85%) isolates were resistant to meropenem and imipenem. Phenotypic tests showed that out of the 100 isolates, 46, 59, 50, 65 and 65 isolates were positive: AmpC disk, CD, EDS, Modified Hodge and Etest MBL, suggesting MBL production, respectively. Gene detection by PCR showed that 23 isolates carried the VIM-1 gene and only three isolates carried the IMP-1 gene. The prevalence of metallo-β-lactamases-containing A . baumanii isolates is increasing, and the coexistence of various carbapenemases is a problem. Continuous monitoring of the infections due to these bacteria should be taken into account in planning for alternative and new therapeutic strategies.

scholarly journals Bacteriological and molecular study of Pseudomonas aeruginosa strains isolated from different clinical cases in Erbil and Kurkuk

2018 ◽  
Vol 41 (2) ◽  
pp. 124-130
Author(s):  
Asif Hasan Abdul Razzaq
Keyword(s):  
Molecular Weight ◽  
Polymerase Chain Reaction ◽  
Virulence Genes ◽  
Antibiotic Sensitivity ◽  
Molecular Study ◽  
Clinical Samples ◽  
Chain Reaction ◽  
Biochemical Tests ◽  
Antibiotic Sensitivity Test ◽  
Polymerase Chain

     This study included isolates of bacteria from 125 clinical samples in Erbil and Kirkuk Hospital including (burns, wounds, urine and sputum); 38 isolates were identified as P. aeruginosa after conducting microscopic and biochemical tests. The results of antibiotic sensitivity test showed that all isolates of P. aeruginosa  were different in resistance to Pipracillin, Erythromycin with rate of (100%) and to the Nalidixic acid (94.73%) while the lowest resistant antibiotics were to Co-trimoxazole, Ceftazidime and Ciprofloxacin, which amounted to (26.31%, 23.68 and 21.05%) respectively. For molecular diagnosis of P. aeruginosa some virulence genes the alg D and exo A were amplified through Polymerase Chain Reaction technique. The results showed that in 38 isolates cases only 22 (57.9%) were positive for algD gene by amplification of 520 bp band. While in urinary tract infection; 6 samples (60%) had alg D gene, and 8 (57.14%) isolates had alg D gene in wounds samples; also 7(70%) isolates from burns had that gene, while the sputum samples showed only one with alg D gene which was the lowest ratio; but in amplification of exo A, the results showed the presence of only one isolate from burns with molecular weight 396 bp with no appearance in others. 

scholarly journals Successful management of Keratoconjunctivitis in goats in Chennai

2018 ◽  
Vol 10 (4) ◽  
pp. 1196-1198
Author(s):  
G.R. Athira ◽  
D. Chandrasekaran ◽  
C.S. Arunaman ◽  
N.R. Senthil ◽  
S. Vaiaramuthu
Keyword(s):  
Tamil Nadu ◽  
Supportive Therapy ◽  
Antibiotic Sensitivity ◽  
College Teaching ◽  
Corneal Opacity ◽  
Purulent Discharge ◽  
Uneventful Recovery ◽  
Eye Drops ◽  
Biochemical Tests ◽  
Antibiotic Sensitivity Test

Present study was undertaken to study the management of keratoconjunctivitis in five non- descriptive female goats presented to Madras Veterinary College Teaching Hospital, Tamil Nadu with the history of gradual clouding of eyes and partial loss of vision after 15 days from kidding. On clinical examination, the present study observed purulent discharge from eyes and congested conjunctival mucous membrane of goats with other clinical parameters which were normal. Eye examination showed creamy white spot and opacity of corneal opacity with absence of reflexes such as menace, papillary light reflex (PLR) and with partial blindness. Hemato biochemical parameters showed normal values except neutrophilia. Sterile ocular swabs were collected and the organism was identified as Morexella bovis based on their colony morphology and biochemical tests. Based on the Antibiotic sensitivity test (ABST), the affected goats were treated with sensitive antibiotics, eye drops and supportive therapy and the affected goats showed clinical improvement followed by uneventful recovery after one week and prevent further complications such as corneal ulceration and blindness, which in turn affects the economy of the farmer.

scholarly journals Identification of Pathogenic Bacteria from Diseased Thai Pangas Pangasius hypophthalmus with Their Sensitivity to Antibiotics

2020 ◽  
pp. 7-21
Author(s):  
Moslima Parven ◽  
M. M. Mahbub Alam ◽  
Sarker Md. Ibrahim Khalil ◽  
Aishi Hamom ◽  
Osman Goni ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Antibiotic Sensitivity ◽  
Pseudomonas Sp ◽  
Biochemical Tests ◽  
Important Species ◽  
High Productivity ◽  
Fresh Fish ◽  
Sugar Fermentation ◽  
Antibiotic Sensitivity Test ◽  
Diseased Fish

Thai Pangas (Pangasiaus hypophthalmus) has been emerging as an economically very important species due their faster growth, year round production and high productivity in South-East Asian aquaculture. It has good market value as a food fish due to its good taste and deliciousness with high protein, minerals and fat content. It is also popular as a game fish. Aims: The present study was conducted to identify bacterial pathogens in diseased P. hypophthalmus and evaluate their sensitivity to antibiotics. Methodology: To identify the causativeagent of diseased fish pure culture of bacteria using slant and streak plate techniques and different biochemical tests such as Gram’s Staining, Motility Test, Sugar Fermentation Test, MR-VP test, Indole Test etc. were performed. To assess the sensitivity of the isolated bacteria to antibiotics five antibiotics disks i.e. Ciprofloxacin (5μg), Azithromycin (15μg), Ampicillin/Sulbactam (20μg), Tetracycline (30μg) and Erythromycin (15μg) were used. Results: Three pathogenic bacteria i.e. Aeromonas hydrophila, Edwardsiella ictaluri and Pseudomonas sp. were identified in the studied diseased fish. Only Pseudomonas sp. were identified from Fresh fish. E. ictaluri was found only in diseased pangas which was the causative agent for thedisease, Bacillary Necrosis. The results of the antibiotic sensitivity test showed multi-resistances of the identified bacteria to the tested antibiotics. The identified bacteria were 100% sensitive to Ciprofloxacin (5μg), intermediate to Azithromycin (15μg) and Tetracycline (30μg), but resistant to Erythromycin (15μg) and Ampicilin/Sulbactam (20μg). Conclusion: Ciprofloxacin (5µg) could be used to control bacillary necrosis disease in Thai pangas. The results of this study will be helpful to the fish farmers for the management of bacterial diseases in fish.

The Characterization of mecA Gene and SCCmec Typing in Clinical Samples of MRSA

2019 ◽  
Vol 13 (2) ◽  
pp. 1
Author(s):  
Ali Jawad ◽  
Aziyah Abdul Aziz
Keyword(s):  
Methicillin Resistance ◽  
Sccmec Type ◽  
Meca Gene ◽  
Teaching Hospitals ◽  
Clinical Samples ◽  
Type I ◽  
Biochemical Tests ◽  
Type Iii ◽  
Sccmec Typing ◽  
Type Iv

MRSA is a major pathogen worldwide and its infections are associated with increased morbidity and mortality, in comparison with other S. aureus infections. The study was designed to characterize the mecA gene and staphylococcal cassette chromosome (SCCmec) in methicillin resistance S. aureus (MRSA). A total of 20 presumptive S. aureus strains were collected from one of the teaching hospitals in Selangor. Using standard biochemical tests, all the isolates were verified as S. aureus. When tested against cefoxitin, 80% of the isolates were confirmed as MRSA. All the MRSA isolates were further subjected to polymerase chain reaction (PCR) to detect the presence of mecA gene. Nine out of the 16 MRSA isolates (56%) were mecA positive, whereas the remaining four were mecA negative. The 16 MRSA with positive mecA gene were further subjected to SCCmec typing of type I, II, III, IV and V. The most frequent SCCmec types were type III (56%) followed by type II (33%), and type IV (11%). None of the isolates were of SCCmec type I or V. Our study indicates that SCCmec type III is predominant among the isolates which is in agreement with other studies conducted on clinical strains of MRSA.  

DETECTION OF BACTERIOPHAGES AGAINST ESKAPE GROUP OF NOSOCOMIAL PATHOGENS FROM GANGA RIVER WATER DURING COMMUNITY BATH AT VARIOUS RITUALS: SINCE 2013–2019

2020 ◽  
pp. 17-21
Author(s):  
Raghvendra Raman Mishra ◽  
Gopal Nath
Keyword(s):  
Water Samples ◽  
Antibiotic Sensitivity ◽  
Assay Method ◽  
Clinical Samples ◽  
Bacteriophage Therapy ◽  
Acinetobacter Baumanii ◽  
E Coli ◽  
Bacterial Lawn ◽  
Phage Sensitivity ◽  
Ganga Water

Introduction: Several species of bacterial contaminants are at the high level in river Ganga water but question arises that, why Ganga water is not spoiled even left for long time and answer is a presence of biological components including bacteriophage and bioactive component such as nanoparticles. Objective: In the present study our aim was to detect bacteriophages of resistant microbes such as ESKAPE group of nosocomial and S. Typhi. from different Ganga water samples collected on different rituals. Material & Methods: This study started since 2013 and completed in 2020. As per study design water sample from different places (Prayagraj, Mirzapur and Varanasi) and sites were collected. A total 210 strains (30 each) of Enterococcus faecium (E. faecium), Staphylococcus aureus (S. aureus), Klebsiella pneumoniae (K. pneumoniae), Acinetobacter baumanii (A. baumannii), Pseudomonas aeruginosa (P. aeruginosa), Escherichia coli (E. coli) ( Called as ESKAPE group) and additionally S. Typhi were identified from the in 500 clinical samples. These identified strains were processed for their biochemical test microscopy and antibiotic sensitivity for its conformation. Confirmed ESKAPE and S. Typhi strains were used for lawn culture. The bacteriophages were isolated from the collected Ganga water samples by using the double layer agar assay method. Results and Discussion: Bacteriophages were observed in the form of plaques on the bacterial lawn culture. Among 210 strains (30 each) of E. faecium, S. aureus, K. pneumoniae, A. baumannii, P. aeruginosa, E. coli and S. Typhi  total 52 phages were detected in the form of plaques on the bacterial lawn culture. Maximum no of phage sensitivity were identified with E. coli (13) then in S. aureus (11). Eight phages of ware specific to S. Typhi and seven were specific to P. aeruginosa and how ever in six phages are specific to K. pneumoniae and E. faecium. Minimum no of phage sensitivity were identified with A. baumanii (1). Conclusion:  Our study concludes that Ganga water is a huge source of above detected bacteriophages among all possible natural sources with full of diversity. This is development of a phage bank, which will be useful for bacteriophage therapy in near future.

scholarly journals Isolation, Identification of Bacterial Species Causing Chronic suppurative Otitis Media and Detection Some of Their Virulence Factors

2019 ◽  
Vol 24 (7) ◽  
pp. 45
Author(s):  
Yumna Shaker Mahmood1 ◽  
Suha Maher Abed1 ◽  
Amar Mohammed Alwan2
Keyword(s):  
Otitis Media ◽  
Virulence Factors ◽  
Bacterial Species ◽  
Age Groups ◽  
Antibiotic Sensitivity ◽  
Chronic Suppurative Otitis Media ◽  
Biochemical Tests ◽  
Suppurative Otitis Media ◽  
Antibiotic Sensitivity Test ◽  
Almost All

The study is conducted to diagnose the aerobic bacterial species causing chronic suppurative otitis media (CSOM), reveal the antibiotic susceptibility pattern and detect some of their virulence factors. Samples were collected during the period from June till December 2018.  From a total of eighty-two patients admitted to Samarra Hospital and outpatient clinics of both genders with different age groups, 82 bacterial culture are recovered using a cotton swab. Identification of bacterial isolates is performed depending on micro and macroscopic cultural characteristics and biochemical tests. Results of the current work show that the highest infection rates are at the age groups >1 to 5 and 11 to 20 years by (20%). Among eight bacterial species isolated in the current study (S. aureus, P. aeruginosa, K.pneumonia, S.epidermidis, E.coli, P.vulgaris, C. freundii, E. Cloacae), S. aureus had scored the highest rate (41%) of the total infections while the lowest rate was scored by E.Cloacae(1%). The antibiotic sensitivity test suggests that almost all isolates were sensitive to ciprofloxacin and meropenem (96% and 94% respectively) while they were resistant to Cefixime. The ability of bacteria is isolated from CSOM to produce biofilm and some virulence factors (gelatinase, hemolysin, DNase, urease) are investigated the virulence factor results revealed that. S. aureus, P.aeruginosa, K. pneumonia had the ability to produce biofilm and S. aureus, P. aeruginosa  have the ability the highest production for the majority of virulence factors.   http://dx.doi.org/10.25130/tjps.24.2019.128

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